• Mycobiology
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• 제30권3호
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• pp.170-174
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• 2002

A total of twenty-nine species and one species variety belonging to 12 genera was isolated from 30 samples of molasses on 1% glucose(10 genera, 22 species and 1 variety) and 50% sucrose(7, 21 and 1) Czapek's agar at $25^{\circ}C$ media. Aspergillus, Mucor, Mycosphaerella and Penicillium were the most common genera on the two types of media. From the above genera, the most prevalent species were: Aspergillus flavus, A. fumigatus, A. niger, Mycosphaerella tassiana, Penicillium chrysogenum, P. oxalicum and P. purpurogenum. Also, some species were only isolated on 50% sucrose such as Eurotium amstelodami, E. chevalieri, E. repens, Humicola fuscoatra, Penicillium aurantiogriseum and P. puberulum. About 65 fungal isolates isolated from 50% sucrose agar were tested for their ability to produce invertase enzyme in liquid medium and 93.8% of the isolates could produce this enzyme. From the positive isolates, 32 showed high invertase activity, 21 had moderate activity and the remaining 8 isolates were of weak activity. Sixty isolates of Aspergillus, Emericella, Eurotium, Mycosphaerella and Penicillium from the preceding study were screened for the presence of their respective mycotoxins. Larva of brine shrimp(Artema sauna L.) were used for toxicity test of the fungal crude extracts. Three isolates out of 60 tested were toxic. Using thin-layer chromatographic technique, 5 different known mycotoxin were detected aflatoxins : B1, B2, G1, G2 and citrinin.

• 대한한의학회지
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• 제22권3호
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• pp.129-140
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• 2001

Objectives : The aim of this study was to evaluate the effects of Punsimgi-eum(fenxingi-yin) in rat model of depression induced by chronic mild stress (CMS). Methods : The change of the consumption of sucrose solution was measured, and the open field test and passive avoidance conditioning test were performed to investigate the anti-depressive effect of Punsimgi-eum(fenxingi-yin). Results : The results were as follows; I. The consumption of sucrose solution was significantly reversed in the test group at 5, 7 and 8 weeks, but there was no significant change in the control group. 2. There was no significant change of body weight after 8 weeks in each group 3. In the open field test, there was significant increase of exploratory activity in the test group. 4. In the passive avoidance test, the test group showed differences in the maintenance of retention from the control group at the 2-week training-testing interval. Conclusions : The present results suggest that Punsimgi-eum(fenxingi-yin) has an anti-depressive effect.

• Journal of Biosystems Engineering
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• 제32권2호
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• pp.102-108
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• 2007

Heat pump systems are recognized to be heating and cooing systems. In this study, to check the practical application possibility of heat pump systems as low temperature storage systems and get basic data, apples of a long term storage items were stored and performance of low temperature storage and quality changes of apples were evaluated. Cooling coefficient of performance of the system was from 1.1 to 1.3. Although ambient air temperature varied widely from $-13^{\circ}C$ to $29.6^{\circ}C$ during low temperature storage period from January to June, the average temperature of low temperature storage chamber was $1.1^{\circ}C$ at setting temperature of $1.5^{\circ}C$. Sucrose of apples stored by the heat pump decreased from initial sucrose of 15.4% (Brix number) to final sucrose of 14.3%. Weight loss ratio of apples was 9.7% and internal and external view of apples after low temperature storage were very satisfactory with the naked eye.

• 대한영양사협회학술지
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• 제12권1호
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• pp.32-43
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• 2006

The purpose of this study is to find out the optimal mixing ratios of three different amounts of pectin, sugar and citric acid for preparation of garlic jam through response surface methodology(RSM) based on the sweetness, pH, color, sensory and texture test. As the sucrose content increased, the sweetness, cohesiveness, color, flavor, gloss and overall palatability of garlic jam tended to be high and the lightness tended to be low. As the pectin content increased, the adhesiveness of garlic jam tended to increase. As the citric acid content increased, the overall palatability of garlic jam tended to be high and the pH tended to be low. The Pectin levels were included 1.8-2.1g, sucrose levels were included 325-342g and citric acid levels were 5.8-6.1g. The optimal mixing ratios of a pectin, sugar and citric acid for manufacturing the best quality of garlic jam were 2.0g, 334g and 6.0g, respectively.

• KSBB Journal
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• 제9권2호
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• pp.133-139
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• 1994

Aureobasidium pullulans를 설탕 및 맥아당을 주 탄소원으로 하는 배지에서 각각 배양하면서 여러 종류의 올리고당류의 합성, 기질 이용성, 그리고 과당 전이효소 및 포도당전이효소의 생산특성에 대해 고찰하였다. 맥아당 배지에 비하여 설탕배지에서 초기 기질의 소비속도가 더 빨랐고 합성된 올리고당류는 두 경우 모두 최대 중합도 6까지의 올리고당류를 생산하였고, 이때 합성된 올라고당류의 최대 농도는 초기 설탕농도 100g/ㅣ 의 경우에서 58g/ㅣ 이었다 이들은 대부분 배양 중에 탄소원으로 다시 이용되었으나 중합도 6의 이소말토올리고당은 기질로 이용되지 않고 배양 말기까지 계속 잔존하였다. 설탕을 주 탄소원으로 하는 배지에서 맥아당은 과당전이효소 및 포도당전이효소의 생산을 촉진시켰으나, 맥아당을 주 탄소원으로 하는 배지에서 설탕은 포도당전이 효소의 생산에 전혀 영향을 미치지 않았다.

• 대한바이러스학회지
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• 제27권2호
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• pp.143-149
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• 1997

Most of the current licenced hepatitis B vaccines are being produced by recombinant DNA technology in large fermentation cultures of Saccharomyces cerevisiae of yeast cells which carry the gene coded for hepatitis B virus surface antigen. These vaccines are proved very effective clinically and the immunogenicity of vaccines could be maintained for a long time under refrigeration. To develope the stabilizer that could increase the stability of hepatitis B virus vaccine which could be stored for a long period at room temperature or higher conditions, glucose, lactose and sucrose solutions in phosphate buffered saline were added into hepatitis B vaccine respectively to make 2.5%, 5%, 7.5% and 10% final concentration in vaccines. These sugar-vaccine mixtures were stored at room temperature for one month, two months and three months respectively and then inoculated into ICR mice intramuscularly. On the fourteenth day after inoculation, mice were bled and sera were tested for the evaluation of efficacies of vaccines. The results showed that 5% glucose, 7.5% lactose and sucrose increased the stability of vaccines in some degree and this method could be applied for the production of other viral vaccines and bacterial vaccines.

• Journal of Ginseng Research
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• 제29권2호
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• pp.113-118
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• 2005

본 연구는 수삼의 저장용기로 많이 이용되고 있는 플라스틱 상자의 통기구 비율 즉 개공율이 수삼의 저장성에 미치는 영향을 조사하기 위하여 개공율을 밀폐(0), 1, 3, 5, 및 $10\%$로 설정하여 실온에서$(19\~23^{\circ}C,\;RH\;40\~61\%)$ 10일간, 냉장으로$(4\~9^{\circ}C,\;RH\;72\~92\%)$ 30일간 저장하면서 이 화학적 변화를 조사한 결과는 다음과 같다. 실온에서 외관변화는 밀폐구에서 4일 부터 곰팡이 발생이 관찰되며 10일째는 부패하였고, $1\%$는 8일부터 곰팡이 발생이 관찰되었다. $5\%$와 $10\%$ 개공율은 4일부터 시들기 시작하였다. 중량감모율은 개공율이 높을수록 많이 나타나 $5\%$와 $10\%$개공율에서 4일후 $10\%$이상 나타났다. 유리당은저장기간의 경과에 따라 maltose와 sucrose가 감소하였는데 개공율이 낮을 수록 많이 감소하였다. 냉장에서 곰팡이 발생은 관찰되지 않았으며 중량모율은 $5\%$와 $10\%$ 개공율에서 12일부터 약 $10\%$를 보이면서 시들기 시작하였으며, 밀폐와 $1\%$는 30일간 저장기간에서도 양호한 외관상태를 보였다. 유리당중 maltose는 감소, sucrose는 감소하였다가 증가하는 경향을 보였다. 이상의 결과에서 보관상자의 개공율을 조절하여 수삼의 저장성을 향상시킬 수 있음을 알 수 있었다.

• 동의신경정신과학회지
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• 제12궈1호
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• pp.123-135
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• 2001

Objective: This study was designed to assess antidepressant effects of Quibitang on an Animal Model of Depression induced by Chronic Mild Stress. Method: The consumption of 1% sucrose solution and active avoidance learning test were used to evaluate antidepressant effect of Quibitang. The consumption of 1% sucrose solution was measured every week for 8 weeks, and active avoidance learning test was executed after 4 weeks treatment of saline or Quibitang. Result: 1. The consumption of 1% sucrose solution was significantly reversed in test group (Quibitang-treated group) at 5th, 7th, 8th weeks, but there was no significant change in control group. 2. Chronic Mild Stress was found to suppress the increase of body weight at 5th, 6th, 7th, 8th weeks. Treatment of Quibitang did not enhanced the body weigt, but it enhanced the consumption of sucrose solution. 3. In order to measure the learning ability of rat which drived to be depressed, we executed active avoidance test. The result revealed that depressed rat showed impaired acquisition than control group, and the treatment of Quibitang restored the learning activity. Conclusion: These results suggest that Quibitang may have antidepressant effects on depression induced by chronic mild stress.

• 한국작물학회지
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• 제45권5호
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• pp.300-304
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• 2000

Tuberous roots of yacon (Polymnia sonchifolia Poeppig ＆ Endlicher) were stored in different temperature and relative humidity conditions after curing for 30 days. Non-decadent percentage, moisture contents, brix degree and sugars were investigated. Whether temperature and relative humidity were high or not, most of the tuberous root decayed during storage, and the decayed percentage was increased as long as the storage period. In final, only 13% of tuberous roots remain intact at 4$^{\circ}C$, 85% relative humidity condition to be appeared best storage condition in this experiment and all of the tuberous root decayed in other storage conditions after 6 months. Moisture contents decreased a little in the high relative humidity. Even though tuberous roots decayed when stored at 1$0^{\circ}C$ and 2$0^{\circ}C$, most of brix degree reached about 17.0. Fructose, glucose and sucrose contents were 1.65, 1.15 and 0.35% at early storage period, and 1.6, 1.1 and 0.5% after 6 months at 4$^{\circ}C$, 85% relative humidity, respectively. In 3 temperature conditions, fructose and glucose increased for a month and then decreased successively afterwards, but sucrose increased gradually during 6 months. Also in 4 relative humidity conditions, fructose, glucose and sucrose contents were changed with similar to that of in temperature. Changes of fructose and glucose were the same except on 4$^{\circ}C$, 85% relative humidity, that was lower level than moisture of tuberous root.

• 원예과학기술지
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• 제35권1호
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• pp.131-141
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• 2017

본 연구는 면마과 가는쇠고사리[A. aristata (G. Forst.) Tindale]의 각 생육단계에 적합한 기내 대량증식법을 구명하기 위해 수행되었다. 포자를 1 / 8 - 1배로 조절한 MS 배지와 Knop 배지에 파종한 결과, Knop 배지에서 최종 발아율이 87.1%로 가장 높았다. 또한 전엽체를 배지의 종류, sucrose와 활성탄의 농도, 질소급원의 농도 및 비율을 다르게 처리하여 8주간 배양하였다. 실험의 결과, 가는쇠고사리의 전엽체는 sucrose 3%의 MS배지에서 $NH_4Cl$과 $KNO_3$의 비율을 20 : 40mM로 하여 총 농도를 60mM로 조절한 배지에서 증식이 가장 왕성하였다. 토양 종류에 따른 포자체 형성을 관찰한 결과, 상토:펄라이트를 2 : 1(v/ v)로 혼용하였을 때, 단위면적 당 발생한 포자체의 수가 73.8개 / $7.5{\times}7.5cm$로 가장 많았다. 반면 피트모스를 단용 및 혼용처리한 조건에서는 전엽체 발달 및 포자체 형성이 억제되었다. 따라서 가는쇠고사리는 MS 배지에서 전엽체를 증식시킨 다음 상토 : 펄라이트(v / v = 2 : 1) 혼합토양에서 포자체 형성을 유도하는 것이 효과적인 증식법으로 생각된다.