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Effects of Medium Components and Composition on Mass Propagation of Arachniodes aristata (G. Forst.) Tindale

가는쇠고사리의 대량번식에 미치는 배지구성물질과 배양토의 영향

  • Cho, Ju Sung (Department of Horticultural Science, Chungbuk National University) ;
  • Han, Ji Hyun (Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University) ;
  • Lee, Cheol Hee (Department of Horticultural Science, Chungbuk National University)
  • 조주성 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단) ;
  • 한지현 (충북대학교 원예과학과) ;
  • 이철희 (충북대학교 축산.원예.식품공학부 생물건강소재산업화사업단)
  • Received : 2016.02.23
  • Accepted : 2016.10.09
  • Published : 2017.02.28

Abstract

This study was conducted to investigate in vitro mass propagation methods suitable for each growth stage of A. aristata (G. Forst.) Tindale, from spore germination to sporophyte formation. Among spores germinated in $1/8-1{\times}MS$ medium and Knop medium, Knop medium yielded the highest germination percentage (87.1%). We cultured prothalli obtained from germinating spores for 8 weeks on media with different concentrations of sucrose and active carbon, as well as different concentrations and ratios of nitrogen, to select a suitable growth medium. A. aristata (G. Forst.) Tindale prothalli grew most actively in MS medium with 3% sucrose and 20 : 40 mM of $NH_4Cl$ and $KNO_3$ (total concentration of 60 mM). We investigated sporophyte formation according to soil type, finding that bedding soil mixed with perlite at a 2 : 1(v / v) ratio yielded the highest number of sporophytes per pot ($73.8/7.5{\times}7.5cm\;pot$). By contrast, when peat moss was used alone or mixed with other substrates, prothallus development and sporophyte formation were suppressed. Therefore, the most effective propagation method for A. aristata (G. Forst.) Tindale is to grow prothalli in MS medium and to induce sporophyte formation in a mixture of bedding soil and perlite (v / v = 2 : 1).

본 연구는 면마과 가는쇠고사리[A. aristata (G. Forst.) Tindale]의 각 생육단계에 적합한 기내 대량증식법을 구명하기 위해 수행되었다. 포자를 1 / 8 - 1배로 조절한 MS 배지와 Knop 배지에 파종한 결과, Knop 배지에서 최종 발아율이 87.1%로 가장 높았다. 또한 전엽체를 배지의 종류, sucrose와 활성탄의 농도, 질소급원의 농도 및 비율을 다르게 처리하여 8주간 배양하였다. 실험의 결과, 가는쇠고사리의 전엽체는 sucrose 3%의 MS배지에서 $NH_4Cl$$KNO_3$의 비율을 20 : 40mM로 하여 총 농도를 60mM로 조절한 배지에서 증식이 가장 왕성하였다. 토양 종류에 따른 포자체 형성을 관찰한 결과, 상토:펄라이트를 2 : 1(v/ v)로 혼용하였을 때, 단위면적 당 발생한 포자체의 수가 73.8개 / $7.5{\times}7.5cm$로 가장 많았다. 반면 피트모스를 단용 및 혼용처리한 조건에서는 전엽체 발달 및 포자체 형성이 억제되었다. 따라서 가는쇠고사리는 MS 배지에서 전엽체를 증식시킨 다음 상토 : 펄라이트(v / v = 2 : 1) 혼합토양에서 포자체 형성을 유도하는 것이 효과적인 증식법으로 생각된다.

Keywords

References

  1. Bang KJ, Ju JH (2002) Effects of indoor relative humidity conditions on the growth of Arachniodes aristata and Pyrrosia lingua in native ferns. J Korean Env Res & Reveg Technol 5:34-38
  2. Bertrand AM, Albuerne MA, Fernandez H, Gonzalez A, Sanchez-Tames R (1999). In vitro organogenesis of Polypodium cambricum. Plant Cell Tiss. Org. Cult. 57:65-69. doi: 10.1023/A:1006348628114
  3. Cox J, Bhatia P, Ashwath N (2003) In vitro spore germination of the fern Schizaea dichotoma. Sci Hort 97:369-378. doi: 10.1016/S0304-4238(02)00152-8
  4. Czaja AT (1921). Uber befruchtung, bastardierung and geschlechtertrennung bei prothallien homosporer Farne. Z Bot 13:545-589
  5. Dyer AF (1979) The culture of fern gametophytes for experimental investigation. In Dyer AF ed, The Experimental Biology of Ferns. Academic press, London, pp. 253-305
  6. Fernandez H, Bertrand AM, Sanchez-Tames R (1999) Biological and nutritional aspects in fern multiplication. Plant Cell Tiss Org Cult 56:211-214. doi: 10.1023/A:1006277229136
  7. Higuchi H, Amaki W (1989) Effect of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propagation. Sci Hort 37:351-359. doi: 10.1016/0304-4238(89)90146-5
  8. Ju JH, Bang KJ (2005) Effects of irrigation interval, medium composition and drainage on the growth response of Korea native fern Rumohra aristata at indoor. J Kor Inst Landscape Archit 32:103-108
  9. Korea Fern Society (KFS) (2005). Illustrated fern native to Korea. Geobook, Seoul
  10. Laurent S, Lefebvre MF (1980) Etude de leffet de differentes carences en elemets mineraux sur la teneur en tanoides de gametophytes de filicinees. Bull Soc Bot Fr Lett Bot 127:119-127
  11. Lee CH (2004) Propagation and technique of masspropagation of Pteridophyta native to Korea. Kor Wild Res Assn 3:91-96
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497. doi: 10.1111/j.1399-3054.1962.tb08052.x
  13. Padhya MA, Mehta AR (1982) Propagation of fern (Nephrolepis) through tissue culture. Plant Cell Rep 1:261-263. doi:10.1007/BF00272634
  14. Perez-Garcia B, Mendoza-Ruiz A, Espinosa-Matias S, Gomez-Pianataro LD (2010) Gametophyte morphology of Platycerium andinum Baker and Platycerium wandae Racif. Micron 41:806-813. doi: 10.1016/j.micron.2010.05.005
  15. Schwabe WW (1951) Physiological studies in plant nutrition XVI. The mineral nutrition of bracken. Ann Bot 15:417-446 https://doi.org/10.1093/oxfordjournals.aob.a083290
  16. Shin HC, Park NC, Choi KO (2010) Light responses and introduction plan of the native evergreen 'Filicales plant' into the interior landscape space. J Kor Soc People Plants Env 13:109-116
  17. Shin SL (2007) Several factors affecting in vitro masspropagation of eight fern species. MS Thesis, Chungbuk Natl Univ Cheongju, Korea
  18. Takahashi N, Hashino M, Kami C, Imaichi R (2009) Developmental morphology of strap-shaped gametophytes of Colysis decurrens : a new look at meristem development and function in fern gametophytes. Ann Bot 104:1353-1561. doi: 10.1093/aob/mcp245
  19. Toole EH and Tottingham WE (1918) The influence of certain added solids upon the composition and efficiency of Knop's nutrient solution. Am J Bot 5:452-461. doi: 10.2307/2435152
  20. Whittier DP (1975) The influence of osmotic conditions on induced apogamy in Pteridium gametophytes. Phytomorphology 25:246-249

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