• Clinical and Experimental Reproductive Medicine
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• 제21권1호
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• pp.69-76
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• 1994

The survival and pregnancy rates were compared between non-frozen embryos and cryopreserved embryos at either pronucleate or 2-4 cell stages using the freezing and thawing techniques being identical in both groups were compared with fresh embryos. 496 embryos were frozen with 1, 2-propanediol and sucrose and 117 2-4 cell stages embryos had been thawed and 79.6 and 66.0% of them respectively were survival. Clinical pregnancy rate was 19.2% for embryos frozen at the pronucleate stage and 19.0% for embryos frozen at the 2-4 cell stages while the pregnancy rate of non-frozen embryos was 21.3%. There were no significant difference in the survival and pregnancy rates of embryos frozen at pronucleate and 2-4 cell stages. The current cumulative pregnancy rate per retrieval in all cycles with frozen zygotes is 35.4 %, consid~ erably higher than observed in single transfers of embryos without cryopreservation(21.3%); predicted pregnancy rate after transfer of all frozen embryos is 43.3 %. It is concluded that firstly, the survival and pregnancy rate of cryopreserved embryos at pronucleate or 2-4 cell stages are very similar to those from their fresh embryos and non-frozen embryos and secondly, cryopreservation substantially enhances pregnancy attainment from in vitro fertilization.

• 한국산업융합학회 논문집
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• 제27권3호
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• pp.487-499
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• 2024

This study was conducted to find out the factors affecting the stages of behavioral change about GMO(n=202 university students). 52.0% of the respondents had no experience in GMO education, the stages of pre-contemplation and contemplation were 35.6% and 29.7%, respectively. Experience and awareness on GMO were the highest at 86.6% and 51.5%, respectively, and 55% of the respondents did not know about the implementation of the labeling system. Positive and negative perceptions on GMO score were 3.41 and 3.22, respectively. The need for the full labeling system of GM foods was 56.4%, the intention not to pay when the price rises due to the system was 70.8%. Regression analysis of factors affecting the stages of behavioral change showed health interest(t=2.10, p<0.05), educational experience(t=2.81, p<0.01), knowledge score(t=2.260, p<0.05), and negative perception(t=2.13, p<0.05). The results of this study suggest that in order to increase the stage of behavioral change in GMO, it is necessary to raise interest in health and knowledge and have the correct perception through GMO education

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2005년도 연구개발 발표회 논문집
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• pp.431-433
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• 2005

• 아동학회지
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• 제10권2호
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• pp.1-18
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• 1989

This documentary study explored the stages of human development and education in the Choson Dynasty with the use of "Non Eu, Ye Gi, So Hak, Tae Gyo Sin Gi, and Dong Yi Bo Gam. The findings were compared with Western writings of a comparable period. It was found that education in the Choson Dynasty was largely concerned with mental attitude and conduct in ordinary life (education for manners and living). Education claimed to stand for unity of knowledge and behavior. It was founded on a Confucian view of education with a saint as the ideal model to be emulated. In the early years, home influence was emphasized. This was later replaced by institutional education. Additional education frequently extended to ten years and even forty or more years for the development of leaders. Based on the above findings. It was concluded that (1) the Western stages of human development exemplified by the Puritans and by Rousseau were concerned only with progressive changes from the postnatal stage to adolescence but the stages or the Choson Dynasty applied to the entire life span, from prenatal life through old age: (2) the developmental stages of males and females were defined differently as a result of differences in educational contents and sex-role distinctions: (3) life span educational objectives were Confucian and the preparatory stages were long coming to fruition in late senescence: and (4) although education was centered on manners and based on Confucianism, much of human development in the Choson Dynasty corresponds to that of modern times.

• 한국초지조사료학회지
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• 제18권4호
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• pp.291-302
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• 1998

식물생장 조절제 Uniconazole-P의 처리가 초지조건에서 목초의 생육억제와 종자생산에 미치는 영향을 검토하였다. 초종은 화본과 목초 2초종(orchardgrass와 timothy)과 두과목초 2초종(red clover와 alfalfa)을 공시하였고, Uniconazole-P의 처리농도는 대조구, 20ppm구, 40ppm구의 3처리로 하여, 화본과 목초는 유수분화기에, 두과목초는 개화시기(開花始期)에서 엽면산포하였다. 본 실험에서 얻어진 결과를 요약하면 다음과 같다. 1. Uniconazole-P의 처리효과는 처리농도, 초종 및 생육단계에 따라서 차이가 인정되었다. 2. Uniconazole-P의 처리는 출수초기의 화본과 목초에서 초장과 간장이 단축되었고, 수중과 엽록소 함량을 증가시켰다. 3. Uniconazole-P의 처리는 종자 결실기의 orchardgrass에서 수수, 1경중, 수중, 1수중 및 종자중을 증가시켰고, timothy에서는 초장의 단축과 수수, 1수중, 수중, 종자중 및 수확지수를 증가시켰다. 4. Uniconazole-P의 처리는 개화초기의 red clover와 alfalfa 2초종 모두 초장과 총절간장을 단축시켰지만, 총분지장과 엽록소 함량을 증가시켰다. 특히 red clover에서는 화수와 화중의 증가가 현저하였다. 5. Uniconazole-P의 처리는 종자 결실기의 red clover에서 초장을 단축시켰고, 화중과 종자중을 증가시켰으며, alfalfa에서는 초장을 단축시키고 화중, 종자중 및 수확지수를 증가시켰다. 6. Uniconazole-P의 처리에 의한 화본과 목초의 종자 생산과정은 1) 출수초기에서 초장과 간장의 단축, 2) 출수초기와 종자 결실기에서 수수와 l수중의 증가, 3) 종자 결실기에서 단위면적당 수중과 종자중의 증가 및 수확지수의 증가로 설명할 수 있다. 7. Uniconazole-P의 처리에 의한 두과목초의 종자 생산과정은 1) 개화초기에서 초장과 총절간장의 단축과 분지수와 총분지장의 증가, 2) 개화초기와 종자 결실기에서 화수와 화중의 증가, 3) 종자 결실 기에서 종자중과 수확지수의 증가로 설명할 수 있다.

• 생물환경조절학회지
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• 제31권4호
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• pp.279-285
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• 2022

딸기 수경재배 시 일사비례제어를 이용하여 생육단계별 적정 누적일사량 기준을 설정하고자 수행하였다. 급액 방법은 외부 일사량을 기준으로 일정 누적 일사량에 도달하면 급액하였고 누적 일사량 기준은 150, 200J·cm^-2과 생육단계에 따라 200J·cm^-2에서 150J·cm^-2로 변경하는 처리를 두었다. 타이머 제어는 대조구로 설정하였다. 월별 평균 급액 횟수는 누적 일사량이 많은 3월에 150 J·cm^-2과 생육단계별(150J·cm^-2) 처리구가 5.6회, 누적일사량이 적은 12월에 200J·cm^-2과 생육단계별(200J·cm^-2) 처리구가 2.7회, 타이머는 3.6-3.8회였다. 수분이용효율은 주당 총 급액량이 적었던 타이머 처리구가 19.8g·L^-1로 일사제어 처리구에 비해 낮았다. 일사제어에 따른 생육 및 과실 특성은 차이가 없었다. 생육단계별 처리구가 총 상품과 수량은 주당 328g, 상품과율 85.3%로 가장 높았다. 딸기 수경재배 시 일사비례 급액제어 방식은 재배기간 동안 동일한 누적일사량 기준보다 생육단계별로 누적일사량을 조절하는 것이 과실의 수량 향상에 도움이 되었다.

• 한국수정란이식학회지
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• 제11권3호
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• pp.217-223
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• 1996

This study was conducted to investigate the effects of co-culture for the development rate to morula /blastocyst stages of early porcine embryos, derived from oocytes matured and fertilized in vitro, with porcine endometrial cell monolayers(PEM) in the two different media, respectively. The rates of embryos developed to 2-, 4-, 8~16-cell and morula /blastocyst stage were 49.6, 40.5, 28.2 and 15.3% in Ham's F-10 with PEM, and 55.3, 45.9, 32.7, and 17.6% in TCM-HEPES with PEM, respectively. The above development rates to morula /blastocyst stages were significantly higher than those of the embryos cultured in the Ham's F-10 and TGM-HEPES without PEM(P<0.05). The in vitro development rates to the morula /blastocyst stage of 1-cell embryos cultured in Ham's F-10 and TCM-HEPES without PEM were 0~1.2%. Especially, most of embryos were observed to arrest the development beyond 4-cell stages. As shown in the above results, the co-culture of in vitro produced porcine embryos with PEM in the two different media enhanced the development of fertilized eggs to morula /blastocyst stages in vitro. However, we didn't find out any differences for the in vitro development to morula /blastocyst stages between Ham's F-10 and TcM-HEPES media.

• Journal of Ecology and Environment
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• 제35권3호
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• pp.195-201
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• 2012

Seasonal variations in the profile and concentrations of essential oil in Zanthoxylum piperitum were investigated by gas chromatography-mass spectrometry. Seasonal changes in the percentages of the main constituents of the essential oil of both leaves and fruits from Z. piperitum varied. Variations in essential oil yield and the amount of monoterpenes and sesquiterpenes in leaves and fruits at different developmental stages were significant. The characteristic content of essential oil in leaves was determined mainly due to the content of monoterpenes, and that in fruits was determined largely due to the sesquiterpenes. Twenty-nine compounds in the oil from Z. piperitum leaves were detected; the major compounds were ${\beta}$-phellandrene (26.90%), citronella (15.32%), ${\beta}$-myrcene (3.24%), ${\alpha}$-pinene (2.79%), trans-caryophyllene (2.66%), and fanesyl acetate (2.30%). The highest yield of oil (43.89%) in Z. piperitum leaves was obtained in May but decreased gradually beginning in June. The yield of essential oil from Z. piperitum leaves during early periods was higher than that during later periods and usually decreased from early maturation stages to subsequent stages. However, in contrast to leaves, the oil yield in Z. piperitum fruit increased in June, and oil yield later in the season was higher than that earlier in the season. These results indicate that the essential oil produced from Z. piperitum leaves at the early developmental stages was stored in leaves, and might be transferred to fruit at the final developmental stages.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 춘계학술발표대회
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• pp.79-79
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• 2001

This study was conducted to measure the total protein, DNA, and RNA contents of corpus luteum(CL) in various stages of estrous cycle and pregnancy. CLs were collected from a local slaughterhouse and stages of the estrous cycle of CL were classified as CL1~2, days 1 to 10; CL3(with/without central cavity), days 11 to 17; CL4, days 18 to 20 by method of Ireland et. al(1980) and stages of the pregnancy of CL were classified as P1~3, months 11~3: P4~6, months 4~6; P7~9, months 7~9 of pregnancy. CL3 with/without central cavity(CC) was identified as described by Kastelic et. al.(1990)-CL with CC, more than 2mm in diameter; CL without CC, less than 2mm in diameter. In total protein content, CL3 with CC was significantly lower than P7~9(p<.05). The total DNA content was lower in CL3 with CC than CL3 without CC and CL4(p<.05). In protein : DNA ratio, CL3 with CC was significantly lower than CL4(p<.05), CL3 without CC was significantly lower than P7~9(p<.05), CL4 was significantly lower than CL3 with CC, P1~3 and P7~9(p<.05). No differences were observed in RNA content, protein:RNA ratio, RNA/DNA of CLs in stages of estrous cycle and pregnancy.

• 한국수산과학회지
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• 제56권2호
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• pp.259-264
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• 2023

Day-night vertical euphausiid distribution was investigated at three stations in the East Sea using a Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS). Three euphausiid species were recognized. Euphausia pacifica was more dominant than Thysanoessa longipes. Euphausiids were collected at Station 1 at night, Station 2 at sunset, and Station 3 during the daytime. At Station 1, calyptopis and furcilia stages were concentrated from the surface to 30 m and 20-40 m, respectively. Juveniles and E. pacifica were distributed in strata shallower than 30 m. At Station 2, calyptopis and furcilia stages were dominant in strata from the surface to 40 m. Juveniles were not recorded in strata at 30-100 m. However, E. pacifica occurred in these strata. At Station 3, calyptopis and furcilia stages occurred in the upper 40 m of strata. E. pacifica was distributed deeper than 100 m and rarely occurred above 100 m. The furcilia stages weakly migrated, whereas the calyptopis stages did not. Juveniles and E. pacifica showed a clear migration pattern. Vertical distribution of euphausiids in the northern East Sea varied by life stage and time of day.