• Journal of Microbiology and Biotechnology
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• v.24 no.9
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• pp.1254-1259
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• 2014

We investigated whether ${\beta}$-galactosidase (${\beta}$-gal)-containing Escherichia coli cells could transfer a galactose to 2-phenoxyethanol, resulting in 2-phenoxyethanol galactoside (PE-Gal). PE-Gal was confirmed by liquid chromatography-mass spectrometry. In addition, we also confirmed that a galactose molecule was covalently bonded with PE during thin-layer chromatography analysis of the ${\beta}$-gal hydrolysate of PE-Gal. The yield for PE-Gal synthesis was about 37.5% (weight basis), which was about 7-8 times greater than that of a previous report. In addition, the concentration of ${\beta}$-gal (0.96 U/ml) used in this PE-Gal synthesis was about 20 times less than that in a previous report.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.653-657
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• 2011

The efficiency of clove oil, MS-222, and 2-phenoxyethanol was evaluated as anesthetics in juvenile Scomber japonicus. Stage A5 of anesthesia was assumed to be sufficient for conducting routine aquaculture procedures in less than 3 min, with recovery (stage R5) in less than 5 min. The lowest effective doses of the three anesthetics were 50 mg $L^{-1}$ clove oil (anesthetic time of 71.3 s and recovery time of 167.0 s), 100 mg $L^{-1}$ MS-222 (anesthetic time of 70.7 s and recovery time of 115.7 s), and 400 mg $L^{-1}$ 2-phenoxyethanol (anesthetic time of 86.7 s and recovery time of 95.0 s). Anesthetic times decreased with increasing doses for all three anesthetic agents, and fish anesthetized with clove oil exhibited the longest recovery times. After 30 min, the highest plasma cortisol and lactate levels were detected with the use of clove oil, whereas the lowest values were observed with 2-phenoxyethanol. In addition, high glucose levels were maintained during recovery with clove oil, but the treatments did not significantly differ. The most effective of the three anesthetic agents was 2-phenoxyethanol, although all were considered acceptable for use in cultures of juvenile Scomber japonicus.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.2
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• pp.127-134
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• 2015

We investigated the growth-inhibitory activities of cosmetics and their preservatives against pathogens and resident skin bacteria. Of the tested cosmetics, preservatives such as parabens, 1,2-hexanediol, phenoxyethanol-contained toner, emulsion, cream and baby cream exhibited potent antibacterial effects against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Parabens, 1,2-hexanediol and phenoxyethanol inhibited the growth of pathogens, as well as skin-resident bacteria such as Staphilococcus epidermidis, Shigella flexneri, Enterobacter aerogenes and so on. The application of a basic cream containing phenoxyethanol to human skin was shown to disturb the skin microbiota: at the phylum level, Proteobacteria increased and at species level, 4P004125_s increased and Propionibacterium humerusii decreased. Based on these findings, parabens, 1,2-hexanediol and phenoxyethanol have antimicrobial activity and cosmetics containing phenoxyethanol may disturb skin microbiota.

• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.423-432
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• 2018

This study was performed to develop a new natural antimicrobial materials by analyzing the effect of extracts obtained from Ten Lauraceae Species on the inhibitory activity against Propionibacterium acnes. The plant materials were collected from Wando and Jeju islands, and the antimicrobial activity of the crude extracts was examined by the agar diffusion method with different part (i.e., leaf and branch), solvents (i.e., distilled water, 80% ethanol, and 100% methanol) and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The control agents used were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 10 species used in the study showed inhibitory activity, which confirmed their antimicrobial action against acnes. Among these, leaves of Laurus nobilis L. which was extracted in 80% ethanol for 45 min showed the largest clear zone (19.8 mm). Leaves of L. nobilis L., showing highest antimicrobial activities among 10 species, were successively reextracted with n-hexane, chloroform, ethylacetate and n-butanol. As a results, in all fractions except butanol, clear zone above 10 mm were formed. The ethyl acetate fraction showed the highest inhibitory activity (13.3 mm) and the inhibitory activity was significantly higher than that of crude extract (10.2 mm) and phenoxyethanol as a control (12.5 mm).

• Environmental Mutagens and Carcinogens
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• v.24 no.2
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• pp.79-84
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• 2004

Three synthetic chemicals, benzoyl chloride, 2-propyn-l-ol, and 2-phenoxy ethanol were selected for genotoxicity testing, based on production quantity and available genotoxic data. In our previous report, benzoyl chloride induced chromosomal aberrations in Chinese hamster lung (CHL) fibroblast in vitro with and without metabolic activation, while 2-propyn-l-ol and 2-phenoxy ethanol induced only with metabolic activation. To compare the genotoxicity of chromosome aberration assay, the single cell gel electrophoresis (comet) assay subjected using CHL cells. As a result, statistically significant differences of tail moment values of benzoyl chloride, 2-propyn-1-ol, and 2-phenoxy ethanol were observed compared with control values on almost all concentrations with S9 or without S9 metabolic activation system. This results suggest that genotoxic results of the comet assay and the chromosome aberration assay show correlationship of genotoxicity in the CHL fibroblast. In summary, the positive result of chromosome aberration of benzoyl chloride, 2-propyn-l-ol, and 2-phenoxy ethanol was also induced DNA damages in comet assay with same cell line. Consequently, comet assay will be useful and more accurate tool to detect and to confirm the genotoxicity especially DNA damages in CHL fibroblast.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.954-961
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• 2017

We investigated the purifications of PE-gal and CPN-gal, synthesized by transgalactosylation reaction using recombinant ${\beta}$-gal. The reaction mixture containing PE and PE-gal was first mixed with EA, and thereafter PE and PE-gal were distributed in two-phase (EA/water) system. In this system, PE and PE-gal was selectively moved into EA and water phase, respectively. Then, the water phase was collected, and silica gel chromatography was carried out using the collected water phase. Finally, we compared two purified PE-gal samples using HPLC and TLC analysis, in which the one was purified only by silica gel chromatography and the other was purified by EA extraction followed by silica gel chromatography. In the latter case, the residual PE was almost completely removed, whereas, in the former case, the residual PE was remained remarkably. Additionally, the purification yield of PE-gal was about 21% on the basis of mole. In the same purification protocol, CPN-gal was able to be purified using EA extraction followed by silica gel chromatography, in which the residual CPN was almost removed when CPN-gal was purified by EA extraction followed by silica gel chromatography.

• Journal of the Korean Applied Science and Technology
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• v.41 no.1
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• pp.1-8
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• 2024

The safety of cosmetic ingredients is considered paramount. In order to enhance safety, a novel preservative, PE-gal, was bio-synthesized by utilizing the Escherichia coli enzyme 𝛽-galactosidase on the conventional preservative 2-phenoxyethanold (PE). The skin absorption of the bio-synthesized product, PE-gal, intended for use in cosmetics, was evaluated for permeability using the Franz Diffusion Cell Assay system, comparing it with the conventinal preservative PE. When using samples of the same mass concentration, the Flux and Kp values of PE increased over time, indicating a gradual increase in permeability. However, PE-gal did not exhibit sufficient permeability to measure. This suggests that the skin permeability of PE is higher than that of the PE-gal saccharide. According to Marzulli et al., when confirming the degree of permeation using Kp values, the permeation rate of PE was measured as "slow" at a concentration of 1mg/mL. Thus, the transdermal permeability of the divedened form of PE-gal was significantly lower compared to PE.

• 한국석유지질학회:학술대회논문집
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• 2005.09a
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• pp.94-100
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• 2005

동해 울릉분지 북서부해역에서 채취한 코어퇴적물을 이용하여 울릉분지의 제4기 후기 층서와 퇴적환경을 연구하였다. 이를 위하여 테프라층서, 탄소연대측정, 퇴적상, 퇴적물의 암질, 퇴적물과 공극수의 원소성분 등을 분석하였다. 퇴적상을 기준으로 코어퇴적물은 Unit 1, Unit II 및 Unit III으로 나누어지며, Unit의 경계부근에서는 주로 화산력으로 구성된 2매의 향암질 또는 조면암질 테프라층과 주로 화산재로 구성된 1매의 유문암질 테프라층이 협재되어 나타난다. 이들 테프라층에는 울릉도에서 기원한 울릉-오키층(Unit I/II 경계, 약 8.1 cal. kyr BC)과 울릉-야마토층(약 30.7 cal. kyr BC), 일본에서 기원한 아이라-탄자와층(Unit I/II경계, 약 22.8 cal. kyr BC)이 있다. Unit II의 중간부근에서 발견되는 DLM(Dark Laminated Mud) 층에는 망간보다는 철이 상대적으로 풍부하고, 철은 주로 구상집적체의 황화철로 발견되는데, 이들 광물은 정체환경 동안 물의 순환이 약화된 시기에 무산소환경에서 형성되었다. 퇴적물 내 유기물은 해양성 기원으로, 열적 성숙단계가 대부분 미성숙단계에 놓여 있다. Termination I 전에 급격하게 총유기탄소 (TOC)의 함량이 증가함을 보여주는데, 이는 빙기에서 간빙기로 변하는 시기에 해수면 변동과 관련이 있는 것으로 보인다. 공극수와 공기층 가스의 분석에 의하면, 황산염($SO_4^{2-}$) 농도는 매몰심도와 함께 증가하고, 반대로 공기층 가스의 주성분인 메탄($CH_4$)의 농도는 감소한다. 이는 미생물학적인 활동과 관련된 황산염 환원작용이 코어 상부에서 일어났으며, 점차 메탄생성단계 (methanogenesis)로 전이가 일어났음을 의미한다.측해본 결과 침몰선체의 강도는 초기강도에 비해 약 2/5정도 감소하였음을 알 수 있었다.할 수 있었다. 이 연구 사례를 통하여 3차원 GPR 탐사 또한 국부적인 이상대의 규명뿐만 아니라 광역적인 고고학 조사에도 다른 물리탐사와 마찬가지로 쉽게 활용될 수 있다는 결론을 얻을 수 있었다. 3차원 GPR 탐사가 향후 국내의 문화재 조사에 표준화된 탐사과정 중의 하나로써 적극 활용되길 기대한다.larity가 높은 oil에서는 약 $70 {\~} 90\%$의 phenoxyethanol이 유상에 존재하였다. 또한, 미생물에 대한 항균력도 phenoxyethanol이 수상에 많이 존재할수록 증가하는 경향을 나타내었다. 따라서, 제형 내 oil tomposition을 변화시킴으로써 phenoxyethanol의 사용량을 줄일 수 있을 뿐만 아니라, 피부 투과를 감소시켜 보다 피부 자극이 적은 저자극 방부시스템 개발이 가능하리라 보여 진다. 첨가하여 제조한 curd yoghurt는 저장성과 관능적인 면에서 우수한 상품적 가치가 인정되는 새로운 기능성 신제품의 개발에 기여할 수 있을 것으로 사료되었다. 여자의 경우 0.8이상이 되어서 심혈관계 질환의 위험 범위에 속하는 수준이었다. 삼두근의 두겹 두께는 남녀 각각 $20.2\pm8.58cm,\;22.2\pm4.40mm$으로 남녀간에 유의한 차이는 없었다. 조사대상자의 식습관 상태는 전체 대상자의 $84.4\%$가 대부분이 하루 세끼 식사를 규칙적으로 하고 있었으며 식사속도는 허겁지겁 빨리 섭취하는 경우가 남자는 $31.0\%$, 여자는 $21.4\%$로 나타났고 이들을 제외한 나머지 사람들은 보통 속도 혹은 충분한 시간을 가지고 식사를 하였다. 평소 식사량은 조금 적게

• The Transactions of the Korea Information Processing Society
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• v.5 no.10
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• pp.2575-2583
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• 1998

The productionof the highly relible softwae systems and theirs performance evaluation hae become important interests in the software industry. The software evaluation has been mainly carried out in ternns of both reliability and performance of software system. Software reliability is the probability that no software error occurs for a fixed time interval during software testing phase. These theoretical software reliability models are sometimes unsuitable for the practical testing phase in which a software error at a certain testing stage occurs by causes of the imperfect debugging, abnornal software correction, and so on. Such a certatin software testing stage needs to be considered as an outlying stage. And we can assume that the software reliability does not improve by means of muisance factor in this outlying testing stage. In this paper, we discuss Bavesian software reliability growth modeling and estimation procedure in the presence of an imidentitied outlying software testing stage by the modification of Jehnski Moranda. Also we derive the Bayes estimaters of the software reliability panmeters by the assumption of prior information under the squared error los function. In addition, we evaluate the proposed software reliability growth model with an unidentified outlying stage in an exchangeable model according to the values of nuisance paramether using the accuracy, bias, trend, noise metries as the quantilative evaluation criteria through the compater simulation.

• Korean Journal of Plant Resources
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• v.31 no.4
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• pp.330-341
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• 2018

This study was performed to investigate and measure the antimicrobial activity of evergreen woody species extracts on Trichophyton mentagrophytes. To do this, leaves and stems were collected from Wando and Jeju islands, and were used for the extraction with different solvents (i.e., distilled water, 80% ethanol, and 100% methanol), and at different ultrasonic extracting times (i.e., 15, 30, and 45 minutes). The experiment was conducted by using the agar diffusion method. The clear zone was measured after incubating the paper disc containing the plant extract in a bacterial culture medium. The controls were synthetic antimicrobials, methylparaben and phenoxyethanol, at concentrations of 0.4, 1, 2, and 4 mg/disc. Altogether, extracts of 56 out of 64 species used in this study had inhibitory activity, which confirmed their antimicrobial activity against Athlete's foot. Among them, the crude ethanolic extract of Elaeocarpus sylvestris in 45 min showed a zone of inhibition < 20.2 mm, while the clear zone of Actinodaphne lancifolia ethanolic extraction for 30 min was 23.5 mm. Also, Quercus acuta, Dendropanax morbiferus and Daphne odora showed clear zones of 28.0 mm (45 minutes ethanolic extraction), 20.5 mm (45 minutes crude methanolic extraction) and 19.7 mm (45 minutes methanolic extraction), respectively. Thus, these results confirm that the extracts of evergreen woody species have therapeutic potential against Athlete's foot, and suggest that in order to extract adequate amounts of antimicrobial substance from the plant sources, ideal extraction condition has to be considered.