• Journal of Plant Biotechnology
• /
• v.42 no.3
• /
• pp.180-185
• /
• 2015

Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

• Journal of Genetic Medicine
• /
• v.6 no.1
• /
• pp.38-55
• /
• 2009

Purpose: This study was undertaken to provide the framework for development of a genetic counseling training program, and an accreditation and certification process suitable for non-M.D. genetic counselors in Korea. Materials and Methods: Global standards of genetic counseling curriculums, training program accreditation (TPA), and the certification process for genetic counselors (CPGC) in the U.S.A and Japan were reviewed, and a questionnaire survey was performed to elicit opinions among health-care providers including physicians, nurses, technicians, researchers, and educators. In addition, input from professional communities, including the Korean Society of Medical Genetics (KSMG) and Institute for Genetic Testing Evaluation, was sought in formulating the framework of this study. Results: Comparison of U.S.A. and Japan educational systems showed similarities in curriculum, accreditation, and certification programs. Analysis of 117 respondents opinions showed a high level of agreement in the area of global standards; 88% indicated that KSMG should be in charge of TPA and CPGC, while 77% favored a certification exam composed of both written exam and interview components. Conclusion: Based upon this study we propose that the KSMG should be in charge of providing the TPA and CPGC for non-MD genetic counselors. Requirements for the entrance to a Master's degree genetic counseling program should be open to successful four year undergraduate students in all areas, provided the candidates demonstrate the abilities to master the graduate level of study in human genetics, clinical genetics, statistics, psychology, and other required subjects. Eligibility for certification should include qualified candidates of genetic counseling with no formally approved education, but a sufficient amount of clinical experience, in addition to accredited program graduates. Certification examinations should be carried out every two years and the certification should be good for five years, as is the case in Japan.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.39 no.10
• /
• pp.979-984
• /
• 2015

In the present paper, we evaluate the effects of the safe-end length and thickness of the similar metal weld (SMW) of safety injection nozzles on stress distributions at the dissimilar metal weld (DMW). For this evaluation, we carry out detailed 2-D axisymmetric finite element analyses by considering four different values of the safe-end length and four different values of the thickness of SMW. Based on the results obtained, we found that the SMW thickness affects the axial stresses at the center of the DMW for the shorter safe-end length; on the other hand, it does not affect the hoop stresses. In terms of the safe-end length, the values of the axial and hoop stresses at the inner surface of the DMW center increase as the safe-end length increases. In particular, for the cases considered in the present study, the stress distributions at the DMW center can be categorized according to certain values of safe-end length.

• Applied Chemistry for Engineering
• /
• v.5 no.6
• /
• pp.1036-1043
• /
• 1994

The silane crosslinking method was applied for the crosslinking of polyethylene (PE). Crosslinking of PE was performed by, first grafting vinyltrimethoxysilane(VTMOS) to the main chain of PE using an extruder at $200{\sim}210^{\circ}C$, followed by exposure to three different silane crosslinking conditions (1. immersed in $80^{\circ}C$ water, 2. at $80^{\circ}C$ air forced convection oven, 3. exposed to air at room temperature ). The thermal characteristic changes of PE resins with respect to the silane crosslinking conditions were studied by measuring the crystalline melting temperature, density and crosslinking reaction rate. Because silane crosslinking was carried out at solid state, crystalline melting temperature, crystallinity, crystal growth rate, crosslinking reaction rate and the change in the density of silane crosslinked PE were affected by crosslinking condition and the type of base resin. The properties of silane crosslinked PE were different from those of Peroxide crosslinked PE which was crosslinked at the molten state. It was found, from the result of DSC analysis, that silane crosslinked linear low density polyethylene(LLDPE) crosslinked at room temperature had no secondary melting peak because the crosslinking reaction proceeds slowly as the crystalline grows. After crystallization, the melting point of PE was lowered by crystalline interruption of crosslinked site.

• Journal of Life Science
• /
• v.17 no.8 s.88
• /
• pp.1068-1074
• /
• 2007

Parvalbumin occurs in various types of cells in the retina. We previously reported parvalbumin distribution in the inner nuclear layer of bat retina. In the present study, we identified the parvalbumin-immunoreactive neurons in the ganglion cell layer of the retina of a bat, Rhinolophus ferrumequinum, and investigated the distribution pattern of the labeled neurons. Parvalbumin immunoreactivity was found in numerous cell bodies in the ganglion cell layer. Quantitative analysis showed that these cells had medium to large-sized somas. The soma diameter of the parvalbumin-immunoreactive cells in the ganglion cell layer ranged from 12.35 to 19.12 ${\mu}m$ (n=166). As the fibers in the nerve fiber layer were also stained, the majority of parvalbumin-immunoreactive cells in the ganglion cell layer should be medium to large-sized retinal ganglion cells. The mean nearest neighbor distance of the parvalbumin-immunoreactive cells in the ganglion cell layer of the bat retina ranged from 59.57 to 62.45 ${\mu}m$ and the average regularity index was 2.95 ${\pm}$ 0.3 (n=4). The present results demonstrate that parvalbu-min is expressed in medium to large-sized retinal ganglion cells in bat retina, and they have a well-or-ganized distributional pattern with regular mosaics. These results should be important as they are applicable to a better understanding of the unsolved issue of a bat vision. This data will help to provide fundamental knowledge for the better understanding of the unique behavioral aspects of bat flight maneuverability.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.17
• /
• pp.7689-7694
• /
• 2015

Background: Bone tumors are neoplasias with a high overall mortality; one of the main factors that reduce survival is their high capacity to develop metastases. It has been reported that finding lung metastases at diagnosis of osteosarcoma (OS), chondrosarcoma (CS) and giant cell tumor of bone (GCTb) is quite common. In this study, we inquire the relationship of metastases caused by these tumors with different clinical and pathological aspects, in order to guide medical personnel in the diagnosis and opportune treatment of metastases or micro metastases. Materials and Methods: We collected data of 384 patients with clinical, radiological and histopathological diagnosis of OS, GCTb and CS that attended the National Rehabilitation Institute (INR) during 2006 to 2014. Chi-square and Fisher's exact tests were performed for data analysis. Results: In the three tumor types, the presence of metastases at diagnosis was variable (p=0.0001). Frequency of metastases was 36.7%, 31.7% and 13.2% for OS, CS and GCTb respectively. The average age had no significant difference (p>0.05) in relation to metastases, even so, patients with OS and GCTb and metastases, were older while patients with CS and metastases were younger, in comparison to patients without metastases. Males had a higher frequency of metastases (68.2%, p = 0.09) in contrast to CS and GCTb, in which the metastases was more frequent in women with 51.9% (p = 0.44) and 57.9% (p = 0.56) respectively. Broadly, metastasis was associated with primary tumors located in the femur (44.4%), followed by the tibia (15.6%); metastases was more frequent when primary tumor of GCTb and OS were in the same bones, but were located in the hip (26.3%) for CS. Conclusions: The frequency of metastases in OS, GCTb and CS is high in our population and is determined by different clinicopathological variables related to the kind of tumor. Further studies are needed in order to evaluate metastases subsequent to diagnosis and associations with survival and clinicopathological factors, as well as to determine the sensitivity and specificity of current methods of detection.

• The Journal of Society for e-Business Studies
• /
• v.23 no.2
• /
• pp.1-20
• /
• 2018

Car Sharing Service is provided with sharing the vehicleas you can use it anywhere and anytime. The paper verified the factors impact between providing infrastructure characteristics such as scarcity, system quality and service quality and user evaluation factors based on DeLone & McLean IS Success Model. User evaluation factors have an influence on net benefit. D&M IS model mainly proved inner organization impact. The research collected experienced car sharing service, we analyzed the 224 respondents. The result of the hypothesis follows. The scarcity of time is negatively related to perceived social support and app trust. Provisional coupon is positively related to perceived social support and app trust. But, scarcity of quantity is not related to user evaluation factors. Most of the system qualities are related to app trust, however, privacy concern isn't related to app trust. the paper's demographic characteristic is lack of experience frequency. User evaluation factors that are perceived social support and app trust are positively related to user satisfaction and WoM intention. User satisfaction is individual impact and WoM intention is organization impact. Two factors are the net benefit in car sharing service. The area of sharing economy should more study about correlation among experimental various factors. Thus, the paper has different significant from antecedent research. because of trying to experimental analysis.

• Journal of agricultural medicine and community health
• /
• v.39 no.1
• /
• pp.14-24
• /
• 2014

Objectives: The purpose of this study was to estimate the prevalence of food allergies and find out food allergens. Methods: Questionnaires were completed by 2,415 subjects in D city, among whom 452 subjects believed that they had food allergy. Skin prick tests were performed on these subjects. The data were analyzed using SAS Version 9.0 and ${\chi}^2$-test. In addition, several recent studies were reviewed. Results: The prevalence rate of food allergic symptoms was 25.6% in males and 25.8% in females, respectively. The positive rate of skin prick tests among those who were allergic to any of 14 food items was 21.4% in males and 14.7% in females(p=0.018). Therefore, it was estimated that the prevalence rate of food allergy was 5.0% in males and 3.6% in females. It was shown that the major food allergens were hop, shrimp, lobster, oyster and egg(whole) in males, and egg(whole), hop, oyster, and pork in females, respectively. The prevalence in recent studies ranged from 1 to 35%. Major food allergens were milk, egg, nuts, fish, shellfish crustacean and fruits/vegetables. Conclusions: In order to provide effective care for food allergy, it is essential to find out the prevalence of food allergies and food allergens.

• Spatial Information Research
• /
• v.18 no.4
• /
• pp.1-11
• /
• 2010

Unlike the previous studies which have focused on 2-dimensional urban characteristics, this paper presents statistical models explaining urban heat island(UHI) effect by 3-dimensional urban morphologic information and addresses its policy implications. 3~dimensional informations of Columbus, Ohio arc captured from LiDAR data and building boundary informations are extracted from a building digital map, Finally NDV[ and temperature data are calculated by manipulating band 3, band 4, and thermal hand of LandSat images. Through complicated data processing, 6 independent variables(building surface area, building volume, height to width ratio, porosity, plan surface area) are introduced in simple and multiple linear regression models. The regression models are specified by Box-Tidwell method, finding the power to which the independent variable needs to raised to be in a linearity. Porosity, NDVI, and building surface area are carefully chosen as explanatory variables in the final multiple regression model, which explaining about 57% of the variability in temperatures. On reducing UHI, various implications of the results give guidelines to policy-making in open space, roof garden, and vertical garden management.

• Journal of the Korea Society of Computer and Information
• /
• v.16 no.2
• /
• pp.153-163
• /
• 2011

Smartphone is a mobile device which can perform better than feature phone. Recently, growth in demand for advanced mobile devices boasting powerful performance, market share of smartphone is increasing rapidly in mobile device market, for example, iphone and android phone. Smartphone can provide many functionalities, e-mail, scheduler, word-processing, 3D-game, and etc, based on its powerful performance. Thus, various secret information is integrated in smartphone. To provide service, sometimes, smartphone transmits informations to outside via wireless network. Because smartphone is a mobile device, user can lose his/her smartphone, easily, and losing smartphone can cause serious security threats, because of integrated information in smartphone. Also data which is transmitted in wireless network can be protected for privacy. Thus, in present, it is very important to keep secure smartphone. In this paper, we analyze threats and vulnerabilities of smartphone based on its environments and describe countermeasures against threats and vulnerabilities.