• Asian-Australasian Journal of Animal Sciences
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• v.26 no.3
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• pp.316-322
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• 2013

In order to evaluate the genetic diversity and discrimination among five Korean native chicken lines, a total of 86 individuals were genotyped using 150 microsatellite (MS) markers, and 15 highly polymorphic MS markers were selected. Based on the highest value of the number of alleles, the expected heterozygosity (He) and polymorphic information content (PIC) for the selected markers ranged from 6 to 12, 0.466 to 0.852, 0.709 to 0.882 and 0.648 to 0.865, respectively. Using these markers, the calculated genetic distance (Fst), the heterozygote deficit among chicken lines (Fit) and the heterozygote deficit within chicken line (Fis) values ranged from 0.0309 to 0.2473, 0.0013 to 0.4513 and -0.1002 to 0.271, respectively. The expected probability of identity values in random individuals (PI), random half-sib ($PI_{half-sibs}$) and random sibs ($PI_{sibs}$) were estimated at $7.98{\times}10^{-29}$, $2.88{\times}10^{-20}$ and $1.25{\times}10^{-08}$, respectively, indicating that these markers can be used for traceability systems in Korean native chickens. The unrooted phylogenetic neighbor-joining (NJ) tree was constructed using 15 MS markers that clearly differentiated among the five native chicken lines. Also, the structure was estimated by the individual clustering with the K value of 5. The selected 15 MS markers were found to be useful for the conservation, breeding plan, and traceability system in Korean native chickens.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1891-1898
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• 2016

Imperata cylindrica, a tall tufted grass which has multiple pharmacological applications is one of the key ingredients in various traditional medicinal formula used in India. Previous reports have shown that I. cylindrica plant extract inhibited cell proliferation and induced apoptosis in various cancer cell lines. To our knowledge, no studies have been published on the effect of I. cylindrica leaf extract on human oral cancers. The present study was undertaken in order to evaluate the anticancer properties of the leaf extract of I. cylindrica using an oral squamous cell carcinoma cell line SCC-9 as an in vitro model system. A methanol extract from dried leaves of I. cylindrica (ICL) was prepared by standard procedures. Effects of the ICL extract on the morphology of SCC-9 cells was visualized by microscopy. Cytotoxicity was determined by MTT assay. Effects of the ICL extract on colony forming ability of SCC-9 cells was evaluated using clonogenic assay. Cell cycle analysis was performed by flow cytometry and induction of apoptosis was determined by DNA fragmentation assay. The ICL extract treatment caused cytotoxicity and induced cell death in vitro in SCC-9 cells in a dose-dependent manner. This treatment also significantly reduced the clonogenic potential and inhibited cell proliferation by arresting the cell cycle in the G2/M phase. Furthermore, DNA fragmentation assays showed that the observed cell death was caused by apoptosis. This is the first report showing the anticancer activity of the methanol extracts from the leaves of I. cylindrica in human oral cancer cell line. Our data indicates that ICL extract could be considered as one of the lead compounds for the formulation of anticancer therapeutic agents to treat/manage human oral cancers. The natural abundance of I. cylindrica and its wide geographic distribution could render it one of the primary resource materials for preparation of anticancer therapeutic agents.

• The Journal of the Korea Contents Association
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• v.10 no.5
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• pp.135-143
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• 2010

As the broadcasting programs produced by VJ are increasing, every broadcasting company has doubts as to how their program evaluations derive the objective evaluation results and proposes their own tailored program evaluation methods considering the change of the production system. The proposed quality evaluation items represent a new model in terms of the perfectness of VJ broadcasting program production and are used as a way to revise the current evaluation methods of the broadcasting companies. To derive the items which help to find the problems of VJ broadcasting programs, 30 specialists, such as PDs, VJs, and professors, are chosen as a specialist group, and the first experiment is performed to select the evaluation items of VJ broadcasting programs. Based on the result of the degree of importance of the evaluation items, 9 VJ program evaluation items are made. Also, the second experiment is performed in order to verify the proposed evaluation criteria. As a result of the experiment, the reliability of the proposed evaluation method is .939, which is proved to be significant. The selection of the quality evaluation items considering only the VJ broadcasting programs turns out to be significant as a new quality evaluation criterion.

• Journal of the Korea Computer Industry Society
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• v.5 no.2
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• pp.227-233
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• 2004

Complex scheduling problems are related to planning, scheduling, constraint satisfaction problems, object-oriented concepts, and agent systems. Human preference-driven scheduling technique was to solve complex scheduling problems using constraint satisfaction problems and object-oriented concepts. We have tried to apply human preference-driven scheduling technique to reservation problems. For customer's satisfaction, we have considered customer's preferences in the reservation scheduling. The technique of reservation scheduling proposed in this thesis is based on object-oriented concepts. 1'o consider the over all satisfaction, the events of every object are alloted to the board along its priority. Constraints to reservation scheduling are classified to global and local. The definition of board and information of every event are global constraints and the preferences to object's board slots are local constraints. We have applied look-ahead technology to reservation scheduling in order to minimize backtracking not to fail the allotment of events.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.4
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• pp.468-476
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• 2006

Hazardous air pollutants (HAPs) have high toxicity and bioaccurnulation potentials into human body even inbsmall amount (levels of ng/$m^3$). As the levels of HAPs might be controversial, it has been become essential to establish the analysis method for correct results. In this study, various analysis methods of VOCs and Aldehydes were compared in order to select the proper methods in our condition. Sampling and analysis method of VOCs were followed to EPA TO-14a and TO-17. VOCs were collected in absorption tube and separated by thermal desorption unit then analyzed by GC/MSD. Aldehydes were sampled in DNPH-cartridge and extracted into solution then analyzed by HPLC as the same condition of EPA TO-13a. This study also shows the results of QA/QC system of selected methods. Some experiments could be improving the data assurance blank test, calibration check, repetition precision check, the determination of detection limit and reproducibility of the retention time. Precisions of VOCs and aldehydes were ranged in 2$\sim$9% and 1$\sim$4% RSD, respectively. Recovery rate of VOCs showed variable ranges from 60 to 133.5%. MDL of VOCs and aldehydes were 0.044$\sim$0.284 ppb and 0.14$\sim$1.02 ng, respectively.

• Microbiology and Biotechnology Letters
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• v.32 no.2
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• pp.101-109
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• 2004

In order to enhance the productivity of AVM $B_{la}$ produced by Streptomyces avermitilis as a secondary metabolite, we established a basic protocol necessary for protoplast fusion with high-producing strains as a fusion partner, and then obtained various kinds offusants by adopting a massive strain-development procedure (a miniaturized strain screening system). An alternative fusion method using UV and/or NTG mutation of protoplasts was developed to screen genetic recombinants without specific selectable markers. In this method, the mutants obtained by protoplast fusion after UV and/or NTG treatment (95% death rate) of the respective fusion partner (protoplasts of the respective mutants resistant against L-isoleucine antimetabolites such as O-methylthreonine and/or azaleucine) were regarded as DNA-recombined protoplast fusants. Notably it was demonstrated that most of the protoplast recombinants obtained by the UV mutation method were able to biosynthesize higher amount of AVM $B_{la}$ , reaching almost three times higher level (almost equal to the industrial productivity), compared to the average AVM Bla amount of the parallel mother strains.

• Korean Journal of Human Ecology
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• v.13 no.1
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• pp.153-158
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• 2004

With considerable development of comfortable and functional clothing in recent years, we need to evaluate the effects of garment pressure in daily wear on each parts of human body because the garment pressure is important to design the clothing. This study was designed to examine the effects of body postures on garment pressure on each parts of human body in the actual clothing conditions. All the data were collected from 50 volunteered subjects. The Garment pressure was measured in lune and December with 8 points CPMS clothing pressure system from scapular, upper am, elbow, under arm, front waist line, side waist line, abdomen, crista ilica, upper hip, middle hip, front thigh, back thigh, front knee and back knee. The postures of subjects were controlled with 3 positions such as standing (posture 1), sitting on the chair (posture 2), and sitting on the floor (posture 3) during measurement of clothing pressure. Clothing weights were more in men than in woman. It showed that clothing weights had no effects on the garment pressure. In this study, however, just the garment pressures on scapular and top of the hip increased significantly by clothing weight (p<. 05). Clothing horizontally pressed on scapular and top of hip but not on other parts. When subjects stood up, the garment pressure was the highest on the side waist. Especially, clothing pressure on the front waist point was lower than that of the left side waist. On the upper parts of the human body, the garment pressure of left side waist was the highest, and followed by front waist, crista ilica, and abdomen in order. When subjects were sitting on the chair, the garment pressure on the lower parts of the human body was the highest on the top of hip. When the subjects were sitting on the chair or on the floor, the surface area on their skin of hip and waist parts increased by postures. In addition, it showed that men felt more comfortable than women on higher clothing pressure level.

• Journal of Korea Multimedia Society
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• v.6 no.5
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• pp.909-920
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• 2003

Nowadays, IT for CRM has been growing and developed rapidly. Typical techniques are statistical analysis tools, on-line multidimensional analytical processing (OLAP) tools, and data mining algorithms (such neural networks, decision trees, and association rules). Among customer data, web log data is very important and to use these data efficiently, applying OLAP technology to analyze multi-dimensionally. To make OLAP cube, we have to precalculate multidimensional summary results in order to get fast response. But as the number of dimensions and sparse cells increases, data explosion occurs seriously and the performance of OLAP decreases. In this paper, we presented why the web log data sparsity occurs and then what kinds of sparsity patterns generate in the two and t.he three dimensions for OLAP. Based on this research, we set up the multidimensional data models and query models for benchmark with each sparsity patterns. Finally, we evaluated the performance of three OLAP systems (MS SQL 2000 Analysis Service, Oracle Express and C-MOLAP).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.14
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• pp.5545-5550
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• 2014

Background: Human biological materials from cancer patients are linked directly with public health issues in medical science research as foundational resources so securing "human biological material" is truly important in bio-industry. However, because South Korea's national R and D project lacks a proper managing system for establishing a national standard for the outputs of certain processes, high-value added human biological material produced by the national R and D project could be lost or neglected. As a result, it is necessary to develop a managing process, which can be started by establishing operating guidelines to handle the output of human biological materials. Materials and Methods: The current law and regulations related to submitting research outcome resources was reviewed, and the process of data 'acquisition' and data 'distribution' from the point of view of big data and health 2.0 was examined in order to arrive at a method for switching paradigms to better utilize human biological materials. Results: For the deposit of biological research resources, the original process was modified and a standard process with relative forms was developed. With deposit forms, research information, researchers, and deposit type are submitted. The checklist's 26 items are provided for publishing. This is a checklist of items that should be addressed in deposit reports. Lastly, XML-based deposit procedure forms were designed and developed to collect data in a structured form, to help researchers distribute their data in an electronic way. Conclusions: Through guidelines included with the plan for profit sharing between depositor and user it is possible to manage the material effectively and safely, so high-quality human biological material can be supplied and utilized by researchers from universities, industry and institutes. Furthermore, this will improve national competitiveness by leading to development in the national bio-science industry.

• Journal of Korea Multimedia Society
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• v.3 no.4
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• pp.389-398
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• 2000

The focus of this study is to investigate how personal display system - Head Mounted Display(HMD) and VDU-affect to the body in virtual environments. This prediction was tested by a comparing of playing 3D games while wearing a HMD and monitoring a VDU for an hour. As quantitative measuring and analyzing tools, SSQ, RSSQ, pre and post posture stability test and vision test are used. The results showed that SSQ and RSSQ total score (TS) are related with the results of pre and post posture stability test and SSQ and RSSQ TS of HMD is higher than TS of VDU by one point five times. Especially, TS of MRG3C, having large field of view, has a possibility by two times of simulator sickness (SS). And in order to investigate the degree of SS regarding to the simulator control condition, two conditions, subjective-passive and subjective-active environments were compared. However, there was no significant difference between two environments. The repetition of the same environment has no effect to reduce the SS. Disorientation and oculomotor scores of the SSQ and RSSQ are higher than that of another symptoms. In addition, RSSQ score of strain/confusion is higher than that of nausea. Therefore, we assume that personal fear could possibly increase the simulator sickness in virtual environments.