• Journal of Aquaculture
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• v.15 no.1
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• pp.31-37
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• 2002

Effect of 2,4-Dichlorophenoxy acetic acid (2,4-D) on vitellogenin (VTG) production and estrogen ($E_2$)-estrogen receptor (ER) binding affinity were examined in primary hepatocyte cultures of rainbow trout, Oncorhynchus mykiss. Hepatocytes were pre-cultured for 2 days; subsequently, $E_2$( 2$\times$$10^{-6}$/ M) and 2,4-D ($10^{-9}~10^{-6}/M$) were simultaneously added to the incubation medium. They were cultured for more than 5 days. VTG and $E_2$-ER binding affinities were analyzed by SDS-PAGE and ELISA, respectively. 2,4-D concentration used had no appreciable effect on the morphology, viability, and DNA content of hepatocytes in culture. It had also no effect on VTG production. However, it interfered with $E_2$-ER binding affinity, which was reduced with increasing concentration of 2,4-D. The affinity was inhibited by 25 and 30% at $10^{-7}$ M and $10^{-6}$ M of 2,4-D, respectively. This result suggested that although 2,4-D had no effect on VTG production, it acted as reno-estrogenic contaminant in ER.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.167.1-167
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• 1994

• Journal of Plant Biology
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• v.34 no.4
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• pp.325-330
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• 1991

The ability of several auxin analogs to induce ethylene production was tested in the corn coleoptile. The synthetic auxins 1-naphthaleneacetic acid (1-NAA) and 2, 4-dichlorophenoxyacetic acid (2, 4-D) had strong stimulatory effects on ethylene induction surpassing that of IAA. Both 2-naphthalaneacetic acid (2-NAA) and 2, 6-dichlorophenoxy acetic acid (2, 6-D), structural analogs of these auxins, respectively, were found to be inactive. Treatment with NPA, a strong inhibitor of polar auxin transport, led to drastic increase in IAA-induced ethylene production while it has bo effect on ethylene production induced by 1-NAA. A positive correlative existed between intracellular auxin level and ethylene production.

• Korean Journal of Ichthyology
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• v.12 no.3
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• pp.173-179
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• 2000

Effects of Estradiol-$17\beta(E_2)$ and 2, 4-dichlorophenoxy acetic acid (2, 4-D) on vitellogenin(VTG) production were investigated in primary hepatocyte culture of olive flounder, Paralichthys olivaceus. Highest survival rate of hepatocyte were observed at $27^{\circ}C$, which markedly declined equal to 50% of those of $15^{\circ}C$. Vitellogenin production peaked at the concentration of $10^{-6}M\;E_2$. No effect was observed on VTG production at various concentrations of 2, 4-D. However, a low concentration of 2, 4-D (ie, $10^{-8}M$) only appeared increased VTG production. $E_2$ or $10^{-8}M$ 2, 4-D-primed VTG production was markedly inhibited by the addition of $10^{-6}M$ tamoxifen to the culture medium(P<0.01). Inhibition was not affected by combinational treatment with $10^{-6}M$ $E_2$ and $10^{-6}M$ 2, 4-D. These results from the current investigation suggest that 2, 4-D mimics $E_2$, but the mechanism of reaction in inducing the $E_2$ receptor are different in VTG production in oliver flounder hepatocytes.

• Journal of Bio-Environment Control
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• v.24 no.1
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• pp.27-33
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• 2015

High temperature stress in summer season at plastic house is a limiting factor for tomato fruit set and yield. This study was performed to assess the effects of gibberellic acid ($GA_3$) and 2,4-Dichlophenoxy acetic acid (2,4-D) spray on fruit set, yield, and quality of tomato cv. 'Adoration'(Enza Zaden Co., Ltd.) under the plastic house in Hwacheon, 2011. Four concentrations (0-, 5-, 10- and $15mg{\cdot}L^{-1}$) of $GA_3$, and three concentrations (0-, 5- and $10mg{\cdot}L^{-1}$) 2,4-D were sprayed in early flowering of tomato in the plastic house, and fruit set, yield and quality characters were observed. The results showed that spray of $10mg{\cdot}L^{-1}$ $GA_3$ significantly increased the fruit set by 14.2% than unsprayed. The spray of $GA_3$ significantly increased the marketable fruit number, fruit weight, and yield. The spray of 2,4-D on blossoms significantly affected the fruit set percentage, fruit weight, marketable fruit weight and yield, and the highest fruit set observed as 62.5% in combined spray of $GA_3$ and 2,4-D at each $5mg{\cdot}L^{-1}$. Fruit size and total soluble solids significantly varied with the concentrations of $GA_3$ sprayed. The result indicates that the spray of $10mg{\cdot}L^{-1}$ $GA_3$ and $5mg{\cdot}L^{-1}$ 2,4-D can be more economic and effective to increase the fruit set, and yield in tomato under high temperature condition in plastic house.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.83-88
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• 2001

The effect of topical application with 200,400 and 600 $\mu$g/ml 2,4-dichlorophenoxyacetic acid (2,4-D) on the fifth larval stadium of the silkworm, B. mori, was analyzed. Larvae treated during fifth larval stadium enhanced larval, cocoon and adult parameters. The larval period was significantly decreased with increase in silk gland weight at 400 and 600 $\mu$g/ml, male cocoon weight and shel1 weight in all the treated groups and filament length and weight at 200 $\mu$g/ml treated group. Length of the ovariole, eggs per ovariole and hatching percentage increased significantly in all the treated groups when compared with those of the carrier control. This suggests that the plant growth regulator 2,4-D in addition to affecting silk production also affect reproductive performance.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.3
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• pp.151-155
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• 2004

The bioremediation of water system contaminated with phenolic compounds having endocrine-disrupting activity, i.e. 2,4-dichlorophenol, 2,4-dichlorophenoxy acetic acid (2,4-D), and 2,4,5-trichlorophenoxy acetic acid (2,4,5-T), was investigated by using ozone oxidation and activated sludge treatment. Ozone oxidation (ozonation time: 30 min) followed by activated sludge treatment (incubation time: 5 days) was an efficient treatment method for the conversion of phenolic compounds in water into carbon dioxide and decreased the value of total organic carbon (TOC) up to about 10% of initial value. Furthermore, 2,4-D was dissolved in water containing salt, i.e. artificial seawater (ASW), and this water was used as model coastal water contaminated with phenolic compounds. The activated sludge treatment (incubation time: 5 days) could consume significantly organic acids produced from 2,4-D in the model costal water by the ozone oxidation (ozonation time: 30min) and decrease the value of TOC up to about 35% of initial value.

• Plant Resources
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• v.3 no.2
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• pp.135-137
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• 2000

Callus cultures from leaf explants of Gypsophila paniculata L. cv. 'Bristol Fairy' have been tested their growth and morphogenic capacity on Murashige and Skoog medium supplemented with 0.l, 0.5, 1 and 3 mg/L 2,4-D. The frequency of callus formation ranged from 43.3% to 100%. The optimal 2,4-D concentration for promoting callus formation and growth was 0.5 to 3 ㎎/L. 4.2∼ 5.6% of adventitious roots were obtained with the use of 0.1 and 0.5 mg/L 2,4-D. Calli grown well on 1.0 mg/L 2,4-D was the heaviest among the calli grown in various concentrations.

• Plant Resources
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• v.3 no.2
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• pp.131-134
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• 2000

Embryogenic callus cultures of Korean native Seosanjong of ginger(Zingiber of officinale Rosc.) were induced through stem explants taken from in vitro shoot-tip cultures. Among the four concentrations of 2,4-D tested in Murashige and Skoog medium, 0.5 and 1 mg/L of 2,4-D was most effective in inducing embryogenic callus. Leaf explants did not express any new morphogenetic response in all 2,4-D concentrations tested. Plantlets transferred to hormone-free MS medium were developed and successfully acclimatized under greenhouse.

• Journal of Plant Biology
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• v.17 no.4
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• pp.163-170
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• 1974

Leaf tissue of Glycine max Merr. was fixed in para-formaldehyde-glutarldehyde and postfixed in osmium tetroxide or postassium permanganate for electron microscopy. The origin of cytolysome-like organelles of mesophyll cell was studied and changes of fine structure of the organelles according to treating solutions such as gibberellin (GA), kinethin (KI), 2,4-dichlorophenoxy acetic acid(2, 4-D) or 2, 4-D＋GA(1mg/l, respectively) were observed. The cylolysome-like organelles differentiate in endoplasmic reticulum and plasmalemma, and they drop into vacuoles being isolated from the formers. They seem to change into myelin-like structure and to be degenerated by autodigestion. Cytolysome-like organelles involved in cell walls and vacuoles showed activity of acid phosphatase. In the group of GA and KI treatment, cytolysome-like organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D＋GA, myelin-like structures increased in size and autodigestion of this organelles were similar to that of the control group. But in the treatmental groups of 2,4-D and 2,4-D＋GA, myelin-like structures increased in size and autodigestion of this organelle seemed to be accelerated. In the treatmental group of 2,4-D＋GA, myelin-like structures shown high electron density were observed in cytoplasm and vacuoles together.