• Title/Summary/Keyword: 1gE

Search Result 9,536, Processing Time 0.05 seconds

Prevalence of Pathogenic Bacteria in Livestock Manure Compost and Organic Fertilizer (가축분퇴비와 유기질비료에서 병원성박테리아의 분포도 분석)

  • Jung, Kyu-Seok;Heu, Sung-Gi;Roh, Eun-Jung;Lee, Dong-Hwan;Yun, Jong-Chul;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.44 no.5
    • /
    • pp.824-829
    • /
    • 2011
  • In recent years, there has been an increasing public concern about fecal contamination of water, air and agricultural produce by pathogens residing in organic fertilizers such as manure, compost and agricultural by-products. Efforts are now being made to control or eliminate the pathogen populations at on-farm level. Development of efficient on-farm strategies to mitigate the potential risk posed by the pathogens requires data about how the pathogens prevail in livestock manure composts and organic fertilizers. Microbiological analysis of livestock manure composts and organic fertilizers obtained from 32 and 28 companies, respectively, were conducted to determine the total aerobic bacteria count, coliforms, Escherichia coli count and the prevalence of Staphylococcus aureus, Bacillus cereus, Salmonella spp., Escherichia coli O157:H7, Listeria monocytogenes, and Cronobacter sakazakii. The total aerobic bacteria counts in the livestock manure composts and organic fertilizers were in the range of 7 to $9log\;CFU\;g^{-1}$ and 4 to $6log\;CFU\;g^{-1}$, respectively. In the livestock manure composts, coliforms and E. coli were detected in samples obtained from 4 and 2 companies, respectively, in the range of 2 to $5log\;CFU\;g^{-1}$ and $2log\;CFU\;g^{-1}$. In the organic fertilizers, coliforms and E. coli were detected in samples obtained from 4 and 1 companies, respectively, in the range of 1 to $3log\;CFU\;g^{-1}$ and $2log\;CFU\;g^{-1}$. In 3 out 32 compost samples, B. cereus was detected, while other pathogens were not detected. In 28 organic fertilizers, no pathogens were detected. The complete composting process can result in the elimination of pathogens in livestock manure compost and organic fertilizer. The results of this study could help to formulate microbiological guidelines for the use of compost in environmental-friendly agriculture. This research provides information regarding microbiological quality of livestock manure compost and organic fertilizer.

Effects on the pathogenicity and the immunogenicity of Eimeria tenella to the chickens treated with dexamethasone and testosterone propionate and on the relation with antibody titers for Newcastle disease virus (덱사메타손과 테스토스테론 호르몬으로 처리된 닭에서 Eimeria tenella의 병원성 및 면역원성과 뉴캣슬병 바이러스에 대한 항체가의 비교)

  • Youn, Hee-jeong;Noh, Jae-wuk;Oh, Hwa-gyun
    • Korean Journal of Veterinary Research
    • /
    • v.35 no.2
    • /
    • pp.337-345
    • /
    • 1995
  • To evaluate the pathogenicity and immunogenicity of Eimeria tenella to the chicken treated with dexamethasone(DEX) and testosterone propionate (TES), we administered 0.1ml/chicken of dexamethasone and 40mg/chicken of testosterone propionate at 1-, 2-, and 7-days old, respectively. We also immunized with ND oil-emulsion vaccine at 2 weeks old. After that, we immunized and challenged with 100 and $1{\times}10^5$ oocysts/chicken of E tenella at 2 and 4 weeks old, respectively. And then we investigated the HI titers for ND virus, survival rate, body weight gain, lesion score and the weight of the bursa of Fabricius and thymus. The titers for ND virus in the groups treated with TES were higher than those in the groups treated with DEX and CON during 3 to 6 weeks. After challenge, the survival rate of testosterone propionate treated-challenged(TES-CHA) and TES-immunized and challenged(TES-V&C) groups were 61.5 and 83.3% and those of the other groups were all 100%. At 1 week after challenge, the lesion scores of TES-CHA group(4.0) was the highest of all experimental groups. Those of DEX and controlchallenged( CON-CHA) groups were 2.8, and those of all V&C groups were 2.4. During 1 and 2 weeks after immunization, the body weight gains of TES groups were severe low(61.6-82.2g and 189.6-260.4g). During 1 and 2 weeks after challenge, the body weight gains of all CHA groups were lower than those of not challenged groups. But, those of all V AC groups were not different from those of not immunized groups. At 4- and 6-weeks old, the weight of the bursa of Fabricius and thymus in the chicken of all TES groups were lower than those of all control (CON) and DEX groups. Therefore, testosterone propionate acted as immunosuppressive drug. Also, it was thought that the chicken affected a little humoral immunity to E tenella.

  • PDF

Physiological Response of Barley to Water Stress and Salt Stress at Seedling Stage (보리 유묘기의 한해와 염해반응)

  • 최원열;박종환
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.42 no.6
    • /
    • pp.693-698
    • /
    • 1997
  • Drought resistance and salt resistance of seedlings were compared with the polyethylene glycol(P.E.G) and NaCl solutions of the same osmotic potential($\Psi_\pi$=-10 bar). Plant height, seedling dry weight, chlorophyll content and leaf water potential decreased while the free proline content increased more in the P.E.G. than in the NaCl solution. Free amino acids increased 2.6 times in the P.E.G. solution and 3.6 times in the NaCl solution more than in the untreated(Hoagland's solution). Free proline occupied 66% and 61% of the content of total amino acids under water stress and salt stress, respectively. Besides free proline, phenylalanine in the P.E.G. solution and phenylalanine, alanine and asparagine in the NaCl solution increased distinctly. In short, it was shown that water and salt stress responses in seedling stage were relatively similar.

  • PDF

Immunohistochemical Prognostic Factors in Soft Tissue Sarcoma (연부조직 육종에서 면역조직화학적 예후인자)

  • Choi, Kyung-Un;Kim, Jeung-Il;Moon, Nam-Hoon
    • The Journal of the Korean bone and joint tumor society
    • /
    • v.14 no.2
    • /
    • pp.106-118
    • /
    • 2008
  • Purpose: Disturbed cell cycle regulatory proteins are key events underlying the development and/or progression of human malignancies. The aim of this study is to evaluate the protein expression status involved in G1/S cell cycle in human soft tissue sarcoma. Materials and Methods: We simultaneously evaluated the expression of Cyclin D1, Cyclin E, CDK4, CDK2, p16, p27, Rb, E2F1, p53 and Ki-67 by immunohistochemistry in 43 cases of soft tissue sarcoma Results: The Cyclin D1, Cyclin E, CDK4, CDK2, E2F1, and p53 were expressed in 25 (58.1%), 18 (41.9%), 13 (30.2%), 33 (76.7%), 20 (46.5%), and 18 cases (41.9%). The p16, p27, and Rb expressions were decreased in 26 (60.5%), 22 (51.2%) and 19 cases (44.2%). All of the cases showed alterations of more than one out of the above proteins. The increased Cyclin E expression and Ki-67 labeling index (LI) were significantly associated with histologic grade. The Cyclin E and E2F-1 expressions were increased in relapsed cases and the CDK4 expression was increased in cases of metastasis. Conclusion: Alterations of G1/S cell cycle regulatory proteins may play an important role in the tumoriogensis of soft tissue sarcomas. Our results suggest that increased expressions of Cyclin E, E2F1, and CDK4 were associated with tumor relapse or metastasis and could be considered as parameters of prognosis of soft tissue sarcoma.

  • PDF

Effect of Salicylate on Antibacterial Activity of Different Antibiotics

  • El-Naggar, Wael A.
    • Archives of Pharmacal Research
    • /
    • v.15 no.1
    • /
    • pp.99-103
    • /
    • 1992
  • Susceptibility of Psudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis to gentimicin and ceforaxime was affected by salicylaye. In presence of salicylaye (15 mM) and gentamicin (1.0 .mu.g/ml), log efficiency of plating (log E. O. P. s) for the tested bacteria were -1.24, -2.17 and -1.66 respectively. The activity of cefotaxime against Bacillus subtilis was reduced (log E. O. P. = 1.33). The highest potentiating effects of salicylaye were shown when using gentamicin against Staphylococcus aureus, cefotaxime against Ps. aeruginosa, log E. O. P.s were -3.0, and -2.4 respectively. On the other hand, no significant effects were detected with cefotaxime against Staphylococcus aureus (log E. O. P. = -0.04). No significant killing was shown in presence of gentamicin or salicylaye alone. There was no significant effect for salicylaye on MICs (By broth dilution) could be observed except in case of gentamicin against Staphyloccus aureus, which was reduced from 0.02 .mu.g/ml to 0.0012 .mu.g/ml. These results raise the concern that high concentrations of salicylaye in patients might interfere with antibiotic therapies.

  • PDF

Production of Soluble Human Granulocyte Colony Stimulating Factor in E. coli by Molecular Chaperones

  • PARK SO-LIM;SHIN EUN-JUNG;HONG SEUNG-PYO;JEON SUNG-JONG;NAM SOO-WAN
    • Journal of Microbiology and Biotechnology
    • /
    • v.15 no.6
    • /
    • pp.1267-1272
    • /
    • 2005
  • The effects of coexpression of GroEL/ES and DnaK/DnaJ/GrpE chaperones on the productivity of the soluble form of human granulocyte colony stimulating factor (hG-CSF) in E. coli were examined. Recombinant hG-CSF protein was coexpressed with DnaK/DnaJ/GrpE or GroEL/ES chaperones under the control of the araB or Pzt-1 promoter, respectively. The optimal concentration of L-arabinose for the expression of DnaK/DnaJ/GrpE was found to be 1 mg/ml. When L-arabinose was added at $OD_{600}$=0.2 (early-exponential phase), soluble hG-CSF production was greatly increased. In addition, it was observed that the DnaK/DnaJ/GrpE and GroEL/ES chaperones had no synergistic effects on preventing aggregation of hG-CSF protein. Consequently, by coexpression of the DnaK/DnaJ/GrpE chaperone, the signal intensity of the hG-CSF protein band in the soluble fraction of cell lysate was increased from $3.5\%\;to\;13.9\%$, and Western blot analysis also revealed about a 4-5-fold increase of production of soluble hG-CSF over the non-induction case of DnaK/DnaJ/GrpE.

Special Right Jacobson Radicals for Right Near-rings

  • Rao, Ravi Srinivasa;Prasad, Korrapati Siva
    • Kyungpook Mathematical Journal
    • /
    • v.54 no.4
    • /
    • pp.595-606
    • /
    • 2014
  • In this paper three more right Jacobson-type radicals, $J^r_{g{\nu}}$, are introduced for near-rings which generalize the Jacobson radical of rings, ${\nu}{\in}\{0,1,2\}$. It is proved that $J^r_{g{\nu}}$ is a special radical in the class of all near-rings. Unlike the known right Jacobson semisimple near-rings, a $J^r_{g{\nu}}$-semisimple near-ring R with DCC on right ideals is a direct sum of minimal right ideals which are right R-groups of type-$g_{\nu}$, ${\nu}{\in}\{0,1,2\}$. Moreover, a finite right $g_2$-primitive near-ring R with eRe a non-ring is a near-ring of matrices over a near-field (which is isomorphic to eRe), where e is a right $g_2$-primitive idempotent in R.

Orientations of Vertical Rift and Grain Planes in Mesozoic Granites, Korea (국내의 중생대 화강암류에서 발달하는 수직의 1번 및 2번 면의 방향성)

  • Park, Deok-Won
    • The Journal of the Petrological Society of Korea
    • /
    • v.16 no.1 s.47
    • /
    • pp.12-26
    • /
    • 2007
  • We have studied orientational characteristics of vertical rift and grain planes developing in 108 quarries for Mesozoic granites. Orientations of these planes vary in different localities. In general, orientations of these planes are predominantly NNE in South Korea. From the regional distribution chart, orientations of these planes show three dominant sets in terms of frequency orders: (1) $N2{\sim}10^{\circ}E(1st-order),\;(2)\;N15{\sim}25^{\circ}E(2nd-order),\;(3)\;N45{\sim}70^{\circ}E,\;N10{\sim}30^{\circ}W\;and\;N70{\sim}80^{\circ}W(3rd-order)$. These granite quarries are classified by the relative difference in the easiness of rock splitting between horizontal and vertical quarrying planes into: R-type, G-type, and H-type. The results showed that quarries for Triassic granites belong to R and G-types;those for Jurassic granites belong to R, G and H-types. In addition, quarries for Cretaceous granites belong mainly to R-type. Among these quarry types, the most diverse type was identified in the quarries for Jurassic granites. R-type (77.8%) shows a higher distribution ratio compared with G and H-types (22.2%). In general, anisotropy of physical properties is found in granitic rocks and there exists close correlation between orientations of granitic rock splitting planes and those of the open microcracks. Meanwhile, it has been reported that preferred orientations of open microcracks suggest maxinum principal stress orientations.

Growth and Characterization of a-Si :H and a-SiC:H Thin Films Grown by RF-PECVD

  • Kim, Y.T.;Suh, S.J.;Yoon, D.H.;Park, M.G.;Choi, W.S.;Kim, M.C.;Boo, J.-H.;Hong, B.;Jang, G.E.;Oh, M.H.
    • Journal of the Korean institute of surface engineering
    • /
    • v.34 no.5
    • /
    • pp.503-509
    • /
    • 2001
  • Thin films of hydrogenated amorphous silicon (a-Si : H) and hydrogenated amorphous silicon carbide (a-SiC:H) of different compositions were deposited on Si(100) wafer and glass by RF plasma-enhanced chemical vapor deposition (RF-PECVD). In the present work, we have investigated the effects of the RF power on the properties, such as optical band gap, transmittance and crystallinity. The Raman data show that the a-Si:H material consists of an amorphous and crystalline phase for the co-presence of two peaks centered at 480 and $520 cm^{-1}$ . The UV-VIS data suggested that the optical energy band gap ($E_{g}$ ) is not changed effectively with RF power and the obtained $E_{g}$(1.80eV) of the $\mu$c-Si:H thin film has almost the same value of a-Si:H thin film (1.75eV), indicating that the crystallity of hydrogenated amorphous silicon thin film can mainly not affected to their optical properties. However, the experimental results have shown that$ E_{g}$ of the a-SiC:H thin films changed little on the annealing temperature while $E_{g}$ increased with the RF power. The Raman spectrum of the a-SiC:H thin films annealed at high temperatures showed that graphitization of carbon clusters and microcrystalline silicon occurs.

  • PDF

Composition of Vitamin A, E, $B_l$ and $B_2$ Contents in Korean Cow's Raw Milk in Korea (국내산 원유 중 비타민 A, E, $B_l$$B_2$ 함량에 관한 연구)

  • Kwak Byung-Man;Kim Sung-Han;Kim Kang-Seob;Lee Ki-Woong;Ahn Jang-Hyuk;Jang Chi-Hoon
    • Food Science of Animal Resources
    • /
    • v.26 no.2
    • /
    • pp.245-251
    • /
    • 2006
  • This study was performed to investigate the changes of vitamin A, vitamin E, vitamin $B_l$ and vitamin $B_2$ contents in cow's raw milk collected from dairy farms in Chungcheong-do and Jeolla-do for a year. The contents of fat soluble vitamin A and E were changed as seasonal effect, but water soluble vitamin $B_l$ and $B_2$ contents were not changed as seasonal effect. Vitamin A content in cow's raw milk was as follows [minimum ${\sim}$maximum (mean), ug/100 mL]; $35.1{\sim}59.0$ (44.4) in spring, $36.7{\sim}65.6$ (50.0) in summer, $28.7{\sim}61.2$ (46.8) in autumn and $29.9{\sim}57.8$ (43.1) in winter. In case of vitamin E was as follows [minimum${\sim}$maximum (mean), ug/100 mL]; $28.3{\sim}59.2$ (45.8) in spring, $39.6{\sim}69.9$ (58.8) in summer, $35.0{\sim}62.8$ (46.2) in autumn and $26.0{\sim}55.4$ (41.5) in winter. In case of vitamin $B_l$ was as follow [minimum${\sim}$maximum (mean), ug/100 mL]; $27.7{\sim}57.9$ (42.84) in spring, $32.4{\sim}66.1$ (49.39) in summer, $34.1{\sim}63.7$ (46.69) in autumn and $20.6{\sim}61.4$ (43.20 in winter. The amounts of vitamin $B_2$ in cow's raw milk was as follows [minimum${\sim}$maximum (mean), ug/100 mL]; $150{\sim}182$ (160 in spring, $145{\sim}185$ (163) in summer, $149{\sim}180$ (166) in autumn and $148{\sim}190$ (167) in winter.