• Title/Summary/Keyword: 1MS/s

Search Result 2,196, Processing Time 0.03 seconds

Anticancer Activity and Quantitative Analysis of Glucosinolates from Green and Red Leaf Mustard (청갓과 적갓에 함유된 Glucosinolates의 항암 활성 및 정량 분석)

  • Kim, Hwal;Kim, Jun-Yeol;Kim, Hyo-Jin;Kim, Do-Kyung;Jo, Hye-Jin;Han, Byoung-Su;Kim, Heon-Woong;Kim, Jung-Bong
    • The Korean Journal of Food And Nutrition
    • /
    • v.24 no.3
    • /
    • pp.362-366
    • /
    • 2011
  • The objective of this study was to compare the anticancer activity of glucosinolates against four different cancer cells; SNU-251, SNU-354, SNU-C4, MCF-7 and to determine the amounts of glucosinolates in mustard leaves. Green and red mustard leaves were cultivated on the field of Rural Development Administration from May to July, 2010. After the leaves were harvest and lypophilized, the fine powder was extracted with boiling 70% methanol(v/v) for the measurement of anticancer activity and then applied to the cancer cells obtained from Korean Cell Line Bank(KCLB). The anticancer activity of red leaf mustard was higher than that of green mustard leaf, particularly in SNU-251 for 24 hr and in SNU-C4 for 48 hr. In both green and red mustard leaf, sinigrin was the most abundant glucosinolte and was determined as 4.71 and 3.06 mg/g dry wt., respectively, but glucoiberin only presented 0.14 mg/g dry wt. with minor amounts.

Heavy Metal Analysis of Inhabitants from City of the Seoul, Korea (서울지역 거주 성인 모발의 유해 중금속 함량 분석)

  • Im, Eun-Jin;Ha, Byung-Jo
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.35 no.1
    • /
    • pp.27-32
    • /
    • 2009
  • Human hair is an excretory system for trace metals and thus metal content in human hair can reflect the body status. The investigation of trace elements in human hair has been correlated with the diagnosis of various diseases as well as the monitoring of deficiency statues in nutrition. Many chronic diseases may be related to mineral status, some may be related to toxic mineral. Hair samples were collected from 120 inhabitants of the city of Seoul, Korea. In this study the concentrations of 10 elements (Hg, Pb, Cd, Al, As, U, Bi, Sb, Ba, Be) in hair were determined by inductively coupled plasma mass spectroscopy (ICP-MS). The conclusions showed that people in Seoul, Korea were affected by some kinds of toxic minerals. The Hg concentrations of male are higher than those of female and reference range. The mean concentration of Sb was higher in the female than male and reference range. In age distribution, the mean concentration of Hg was in 40's are higher than 20's and 30's and reference range. The concentrations of Al were the highest in the 20's. After analyzing, we concluded that a compounded treatment should be conducted, which considers the variety of factors related to detoxification.

Selection of a Triploid Poplar by Flow Cytometric Analysis and Growth Characteristics of its in vitro Grown Plants (유세포 분석을 통한 현사시나무 3배체 선발 및 계통별 기내생장 특성)

  • Bae, Eun-Kyung;Lee, Hyoshin;Lee, Jae-Soon;Noh, Eun-Woon
    • Journal of Korean Society of Forest Science
    • /
    • v.101 no.2
    • /
    • pp.291-296
    • /
    • 2012
  • Triploids are a useful tool for biomass production and molecular breeding of trees with a long life span. Triploids of the poplar 'Hyunsasi' (Populus alba ${\times}$ P. glandulosa) have been developed by crossing between female diploids and a male tetraploid. The tetraploid was developed around the 1970s at Korea Forest Research Institute by colchicine-induced chromosome doubling. Seedlings of the $F_1$ generation were analyzed using flow cytometry to verify their ploidy status. The mean relative fluorescence index of 3 F1 poplars, labeled as Line- 1, Line-17, Line-18, were approximately 1.5 times higher than those of diploid poplars, and the results clearly indicated that they were triploids. The phenotype of the F1 poplars included larger leaves and thicker stem than diploids, and abnormal leaf morphology, especially in the triploid 'Line-18'. Three triploid lines developed roots more slowly and had less roots than diploid. However, 3 poplar cytotypes (2x, Line-1, Line-17) rooted within 10 days on MS medium. In contrast, compared with the 3 cytotypes, the Line-18 showed about 80% and 70% in the rooting rate and the number of roots. The triploid poplars could be directly utilized for biomass production and with their sterility, they could serve as basic material for genetic transformation. In addition, flow cytometric analysis proved to be an effective and reliable method for screening forest trees for their ploidy level.

Chemical Composition of Several Herb Plants (서양 허브식물의 화학성분)

  • Oh, Moon-Hun;Whang, Hea-Jeung
    • Korean Journal of Food Science and Technology
    • /
    • v.35 no.1
    • /
    • pp.1-6
    • /
    • 2003
  • Chemical compositions of several herbs (rosemary, mint, thyme, sage, and lavender) cultivated in Korea were analyzed. Approximate compositions were as follows: moisture $69.92{\sim}82.10%$, crude ash $2.48{\sim}6.15%$, crude fat $0.40{\sim}2.46%$, crude protein $0.84{\sim}1.57%$, and crude fiber $2.48{\sim}6.15%$. Total contents of phenolics determined by Folin-Dennis's method were in the range of $73.24{\sim}197.79mg%$. Contents of minerals, Na, Ca, Mn, P, Mg, Zn, and Fe determined by ICP-AES were $43.0{\sim}112.5,\;177.5{\sim}304.0,\;0.5{\sim}1.5,\;74.0{\sim}218.5,\;57.0{\sim}116.0,\;1.0{\sim}2.0$ and $3.0{\sim}5.0mg%$, respectively. Free sugar contents determined by HPLC were: sucrose $0{\sim}7.61$, glucose $0.94{\sim}15.92$, and rhamnose $0.64{\sim}7.99mg%$. Fatty acids including palmitic, stearic, oleic, linoleic, linolenic, and arachidonic acids were identified by GC. Linoleic and linolenic acid contents were higher than those of palmitic and stearic acids. Aroma components identified by GC-MS were 1,8-cineole, ${\alpha}-phellandrene,\;{\alpha}-terpinene,\;{\beta}-pinene,\;{\beta}-thujone$, borneol, butan-1-ol, cis-sabinene hydrate, ${\delta}-carene,\;{\gamma}-terpinene$, and verbenone.

A Study on Fast Iris Detection for Iris Recognition in Mobile Phone (휴대폰에서의 홍채인식을 위한 고속 홍채검출에 관한 연구)

  • Park Hyun-Ae;Park Kang-Ryoung
    • Journal of the Institute of Electronics Engineers of Korea SP
    • /
    • v.43 no.2 s.308
    • /
    • pp.19-29
    • /
    • 2006
  • As the security of personal information is becoming more important in mobile phones, we are starting to apply iris recognition technology to these devices. In conventional iris recognition, magnified iris images are required. For that, it has been necessary to use large magnified zoom & focus lens camera to capture images, but due to the requirement about low size and cost of mobile phones, the zoom & focus lens are difficult to be used. However, with rapid developments and multimedia convergence trends in mobile phones, more and more companies have built mega-pixel cameras into their mobile phones. These devices make it possible to capture a magnified iris image without zoom & focus lens. Although facial images are captured far away from the user using a mega-pixel camera, the captured iris region possesses sufficient pixel information for iris recognition. However, in this case, the eye region should be detected for accurate iris recognition in facial images. So, we propose a new fast iris detection method, which is appropriate for mobile phones based on corneal specular reflection. To detect specular reflection robustly, we propose the theoretical background of estimating the size and brightness of specular reflection based on eye, camera and illuminator models. In addition, we use the successive On/Off scheme of the illuminator to detect the optical/motion blurring and sunlight effect on input image. Experimental results show that total processing time(detecting iris region) is on average 65ms on a Samsung SCH-S2300 (with 150MHz ARM 9 CPU) mobile phone. The rate of correct iris detection is 99% (about indoor images) and 98.5% (about outdoor images).

Studies on the Volatile Flavor Compounds of Sesame Oils with Roasting Temperature (볶음온도에 따른 참기름의 휘발성향기성분 변화)

  • Kim, Hyeon-Wee;Park, Ki-Moon;Choi, Chun-Un
    • Korean Journal of Food Science and Technology
    • /
    • v.32 no.2
    • /
    • pp.238-245
    • /
    • 2000
  • This study was investigated to compare the changes of flavors in sesame oil with roasting temperature $(110^{\circ}C{\sim}230^{\circ}C)$. In the results of analyzing the volatile flavor compounds of sesame oil with GC and GC/MS, 26 pyrazines, 11 pyridines, 9 thiazoles, 6 furans, 8 pyrroles, 5 phenols, 8 aldehydes, 8 hydrocarbons, 7 alcohols, 2 indoles, 3 ketones, 10 acids, 4 nitriles, 7 esters, and 5 others were isolated, identified, and quantified. The total amount of flavor compounds was increased with roasting temperature. Detected flavors could be devided into top(peak No. $1{\sim}91$), middle$(92{\sim}197)$ and last note$(198{\sim}224)$ by rentention time. The top notes(initial content 19.87 ppm) which contain pyrazines and provide representative roasted flavors were increased significantly with roasting temperature. Initial content of middle note(17.72 ppm) was increased to 36.71 ppm at $170^{\circ}C$, to 95.61 ppm at $220^{\circ}C$, and to 138.62 ppm at $230^{\circ}C$. Last note was almost unchanged up to $170^{\circ}C$ and increased at $190^{\circ}C$, whereas it indicated a tendency to decrease at $230^{\circ}C$. Pyrazines such as methylpyrazine, 2,5-dimethylpyrazine, 2,6-dimethylpyrazine, trimethylpyrazine, 2-ethyl-3,5-dimethylpyrazine which indicate the major components among volatile flavors were increased slightly up to $150^{\circ}C$ and revealed the higher increase than any other components above $170^{\circ}C$. This tendency was also similar to pyridines, thiazoles, and furans. Most of these compounds are assumed to be developed by thermochemical reactions of sesame components by roasting above $170^{\circ}C$. It seemed that a lot of increase in phenols above $210^{\circ}C$ resulted from the production of guaiacol. Acids were almost unchanged up to $190^{\circ}C$, increased at $210^{\circ}C$, and then decreased above $220^{\circ}C$. It seemed to be resulted from pyrolysis of free fatty acids formed from thermal oxidation of oil.

  • PDF

Change of Water Quality and Growth of Leiocassis ussuriensis Cultivated in a Biofloc System using Bacillus subtilis (Bacillus subtilis을 활용한 바이오플락 시스템에서 사육한 대농갱이(Leiocassis ussuriensis)의 성장 및 사육수 수질 변화)

  • Kyu Seok, Cho;Jong Ho, Park;Han Seung, Kang
    • Journal of Marine Life Science
    • /
    • v.7 no.2
    • /
    • pp.196-204
    • /
    • 2022
  • This study observed changes in survival, growth performance and water quality for 90 days to confirm the possibility of Leiocassis ussuriensis farming using Biofloc technology (BFT) using Bacillus subtilis. Feed and molasses were added to the experimental tank to produce BFT water before planting the experiment, and B. subtilis was inoculated to stabilize the water quality for 40 days. The survival rate of the experimental fish was 92.7±3.2% in the control group and 95.8±3.3% in the BFT group. The Weight gain (WG) was 118.1±9.0% of the control and 197.7±15.6% of the BFT, and the Specific growth rate (SGR) was 0.87±0.5% of the control and 1.21±0.06% of the BFT. As for the feed efficiency, the control was 43.7±2.6% and the BFT was measured at 70.1±4.1%, indicating that the feed efficiency of the BFT was higher. As a result of measuring the water quality change during the experimental period, pH was reduced in both the control and the BFT, and Mixed Liquor Suspended Solids (MLSS) did not show any change in the control, but the BFT showed a significant increase at 90 days. NH4+-N and NO2--N showed a significant increase from the 30 days of the experiment in the control, but showed no change in the BFT. In conclusion, as a result of applying the BFT system using B. subtilis to the process of cultivating Leiocassis ussuriensis, the water quality tended to stabilize, and the growth rate and feed efficiency were found to be higher than those of the control, confirmiWng that it had a positive effect.

A Study on the Noises of Fishes (어류가 내는 소리에 관하여)

  • CHO, AM;CHANG, Jee-won
    • Journal of the Korean Society of Fisheries and Ocean Technology
    • /
    • v.8 no.1
    • /
    • pp.14-22
    • /
    • 1972
  • For the development of acoustic fishing method, the noises of fishes have been recorded and analy/'ed by many scientists. Some specimens of fishes were selected as such Cyprinus carpio, Ctenopharyngodon idellus Carassius carassius, and pagrosol1ms major in this experiment. The noises such as feeding noise, driving away noise, jumping noise and fi llip noise were recorded by the tape recorder, Sony Model 262, through the underwa te r microph I one, Oki ST 6582, and analyzed in frequencies bv octave band analyzer, Rion SA-55, and sound pressure level of source by sound level meter, Rion NA-opNN The supplied feed was placed within 5em apart from the hydrophone. The result of analyzed noises were as follow. Cyprinus carjJio; Feeding noise 250- 500 cps, 92- 99 dB Driving away noise 125-2, 000 eps, 101-112 dB Jumping noise 125-2, 000 eps, 99-116.5 dB Ctenopharyngodon idcllus; Driving away noise 125-1, 000 cps, 96-109 dB Carassius carassius; Feeding noise 250- 500 cps, 91. 5- 99.5 dB Driving away noise 125-1, 000 eps, 99-108 dB Carassius auratus Feeding noise 250 eps, 94-101 dB Driving away noise 125-1, 000 cps, 98-110 dB Pagrosomus major Feeding noise 230-500 cps, 90-101 dB Fillip noise 500 cps, 98-108 dB (1) Feeding noise was produced as like as snap noise of twig and gulping down saliva noise in human and dominant frequency range of the noise is 250-500 cps and noise level 90-101 dB. (2) It was found that feeding noise were not a monotonic but a complex tones though fish took the same food. (3) Driving away noise was produced not so keen and the wave form of the noise is rising very sharp and big amplitude in the oscillograph. Dominant frequency range of this noise was about 150-1, 000 cps and noise level 96-112 dB except thut of carp. (4) The frequency of snapper's fillip noise, when it produced by caudal fin in swimming at the surface of water, was 500 cps and noise level 93-108 dB snd that of jumping noise of carp about 150-2, 000 cps and noise level 99-116.5 dB.

  • PDF

Fragrance, Chemical Composition and Toxicity of the Essential Oil in Erect Bur-marigold (Bidens tripartita L.) (가막사리 (Bidens tripartita L.) 정유의 향취, 화학성분 및 세포독성)

  • Yun, Mi-Sun;Yeon, Bo-Ram;Cho, Hae-Me;Lee, Sa-Eun;Jhoo, Jin-Woo;Jung, Ji-Wook;Park, Yu-Hwa;Kim, Song-Mun
    • Korean Journal of Weed Science
    • /
    • v.32 no.3
    • /
    • pp.195-203
    • /
    • 2012
  • The essential oil was extracted by steam distillation from the aerial part of erect bur-marigold (Bidens tripartita L.), one of the noxious weed in paddy field. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The fragrance of the essential oil was green, herbal, oily, spicy. There were 42 constituents in the essential oil:17 hydrocarbons, 6 alcohols, 6 acetates, 5 N-containing compounds, 3 ethers, 3 ketones, 1 lactone and 1 S-containing compound. Major constituents were ${\alpha}$-phellandrene (22.50%), ${\alpha}$-pinene (22.21%), 2,4-dimethyl (2,5-dimethylphenyl) methyl ester benzoic acid (15.11%), limonene (10.66%), ${\beta}$-pinene (35.43%), and ${\beta}$-cubebene (5.27%). The $IC_{50}$ value in MTT assay using HaCaT keratinocyte cell line was 0.018%. However, attachment of patch with 0.1% of the erect bur-marigold essential oil for 24 hr did not show any skin toxicity. Overall results of this study suggest that the essential oil of erect bur-marigold could be used as a source for the development of perfumery industrial products.

Effects of Cytokinin and Auxin on Organ Formation in Leaf Scale Tissue of Allium Sativum L. (Cytokinin과 Auxin이 Allium Sativum L.의 인엽배양(鱗葉培養)에서 기관(器官)의 분화(分化)에 미치는 영향(影響))

  • Park, Kyeong Han;Lee, Yeong Bok
    • Korean Journal of Agricultural Science
    • /
    • v.7 no.2
    • /
    • pp.65-76
    • /
    • 1980
  • In order to determine the effects of cytokinin and auxin on organ formation from tissue of garlic cloves, leaf blades and basal tissues contained meristem of garlic (Allium sativum L.) cloves harvested in 1979 (old cloves) and 1980 (new cloves) were explanted on a MS medium contained various levels of BA ($N^6$-benzyl amino purine), NAA (naphthalene acetic acid), and 2, 4-D (2, 4-dichlorophenoxyacetic acid). And some of the new cloves were explanted on a media contained BA and NAA after chilling treatment at $4^{\circ}C$ for 10, 20, 30 and 40 days. 1. In a culture of leaf blades of old cloves, shoots were differentiated on a medium supplemented with 2mg/l of BA and NAA. 2. Callus was grown as a quite straw-coloured globular mass on a medium contained 0.2 or 2mg/l 2.4-D. 3. As subcultures of globular calli, shoots and roots were differentiated on a medium contained 2mg/l BA and 0.5 or 1 mg/l NAA, whereas no shoots was shown on a conterol. 4. Shoots were differentiated in a culture of leaf blades of new cloves, but they were not in an old cloves in control, and better effect was shown on a medium contained 2mg/l BA and 1mg/l NAA. However shoots were no differentiated from leaf blades chilled at $4^{\circ}C$ for 30 or 40 days at the same condition. 5. Large numbers of adventitious shoots could be obtained from basal region of garlic cultured on a medium contained 1mg/l BA and 4mg/l NAA, or 2mg/l BA and 2mg/l NAA.

  • PDF