• Title/Summary/Keyword: 16S-23S ITS

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Geminocystis urbisnovae sp. nov. (Chroococcales, Cyanobacteria): polyphasic description complemented with a survey of the family Geminocystaceae

  • Elena Polyakova;Svetlana Averina;Alexander Pinevich
    • ALGAE
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    • v.38 no.2
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    • pp.93-110
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    • 2023
  • Progress in phylogenomic analysis has led to a considerable re-evaluation of former cyanobacterial system, with many new taxa being established at different nomenclatural levels. The family Geminocystaceae is among cyanobacterial taxa recently described on the basis of polyphasic approach. Within this family, there are six genera: Geminocystis, Cyanobacterium, Geminobacterium, Annamia, Picocyanobacterium, and Microcrocis. The genus Geminocystis previously encompassed two species: G. herdmanii and G. papuanica. Herein, a new species G. urbisnovae was proposed under the provision of the International Code of Nomenclature for algae, fungi, and plants (ICN). Polyphasic analysis was performed for five strains from the CALU culture collection (St. Petersburg State University, Russian Federation), and they were assigned to the genus Geminocystis in accordance with high 16S rRNA gene similarity to existing species, as well as because of proximity to these species on the phylogenetic trees reconstructed with RaxML and Bayes methods. Plausibility of their assignment to a separate species of the genus Geminocystis was substantiated with smaller cell size; stenohaline freshwater ecotype; capability to complementary chromatic adaptation of second type (CA2); distinct 16S rRNA gene clustering; sequences and folding of D1-D1' and B box domains of the 16S-23S internal transcribed spacer region. The second objective pursued by this communication was to provide a survey of the family Geminocystaceae. The overall assessment was that, despite attention of many researchers, this cyanobacterial family has been understudied and, especially in the case of the crucially important genus Cyanobacterium, taxonomically problematic.

A Study on Typology of Maru's Placement in Korean Traditional Single Houses of Four Kan in Chonnam Province (전라남도 4칸 홑집의 마루배치에 관한 조사연구)

  • Chang, Dong-Kuk;Chung, Seong-Kyoon;Shin, Woong-Ju;Eun, Chul-Young;Kim, Soo-In;Park, Kang-Chul
    • Journal of architectural history
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    • v.16 no.4
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    • pp.23-36
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    • 2007
  • Many researches on the typology of 'Maru' (Wooden deck) in Korean traditional houses have mainly focused on the high-class houses in the central region of Korea. They were mainly concerned on defining Maru's typology by social status of the owner, based on the researches regarding its physical and spatial properties. Maru in the high-class traditional houses has been served as an essential architectural feature showing the social status of owners as well as adapting in the region of humid and hot summer. This research investigated the typology of Maru's placement in traditional single houses of four Kan in Chonnam province, which shows many differences in its placement according to the regions. Research results show that the typology of Maru's placement and openness is different with the location of houses within the region. This difference affects on the circulation of movement, which eventually affects on the pattern of space use. The difference is also very likely to be affected by the natural environment of geography and climate of the region.

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A Study on the Ichthyofauna of Tongbok Lake and its upper Streams from Autumn to Winter (동복호(同福湖)와 그 상류수계(上流水系)의 추(秋).동계(冬季) 어류상(魚類相)에 관한 연구(硏究))

  • Choi, Chung-Gil;Hwang, Yeong-Jin;Park, Jong-Cheon;Ra, Myeong-Suk;Lee, Jong-Bin
    • Korean Journal of Ichthyology
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    • v.6 no.1
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    • pp.28-38
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    • 1994
  • The ichthyofauna was investigated in Tongbok Lake and its tributaries from October, 1992 to January, 1993. Twenty-nine species representing 23 genera and 10 families were collected. Twenty-eight species in 22 genera and 9 families were from the stream area, while sixtween species in 14 genera and 7 families were from the lake area. Among these 14 species were classified as endemic species in Korea. Dominent species in the stream area were Zacco temmincki(28.04%) and Z. platypus (16.76%), whereas the lake area was overwhelmingly dominated by Hypomesus olidus(55.22%) and Z. platypus(44.31%). In the stream area. species diversity indices, evenness indices and dominant indices were 1.056, 0.730 and 0.270, respectively. In the area, the indices for species diversity, evenness and dominance were 0.314, 0.261 and 0.739, and those of body weights were 0.361, 0.300 and 0.700, respectively.

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The Kinematic Analysis of Back-Kick Motion in Taekwondo (태권도 숙련자와 미숙련자의 공격뒤차기 동작에 대한 운동학적 분석)

  • Lee, Dong-Jin;Park, Chan-Ho;Kim, Hun-Soo
    • Korean Journal of Applied Biomechanics
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    • v.16 no.3
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    • pp.43-51
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    • 2006
  • The purpose of this study was to analyze kinematic variables during turing back kick motion of Taekwondo. The subjects of this study were the 4 skilled and 4 unskilled of male university player in respectively. The experiment of this study was used two 16mm high speed cameras and its speed 125 frames/s. Analysis of this data was three dimensional cinematography using KWON3D program package. The results were as following; 1. In the elapsed time, there was no significance difference statically between a skilled and unskilled group. But skilled group was more fast during the motion of I phase. And unskilled group was more fast during the motion of II phase so called force production section, which had an influence on Diechagi's velocity. 2. In the center of gravity of human body, the changing of it was $1.10{\pm}0.04m$, $1.12{\pm}0.03m$ of LFM(left foot movement) and $1.36{\pm}0.08m$, $1.39{\pm}0.09m$ of RKF(right knee flection), and $1.44{\pm}0.08m$, $1.42{\pm}0.09m$ of RFI(right foot impact). There was no significance difference statically between the two groups. 3. The velocity of heel on impact was 1.13m/s in the skilled group and 1.23m/s in the unskilled group, when each angle of knee was $110.4{\pm}10.9deg/s$, $114.8{\pm}28.4deg/s$. The maximum velocity of each performance was reached before the RKF, and the velocity and angle at impact along by two groups did not show any significant difference statically. 4. In the angular velocity of just RKF of lower leg, there was significance difference statically between the two groups(p<.05).

Causal Agents of Blossom Blight of Kiwifruit in Korea

  • Lee, Young-Sun;Han, Hyo-Shim;Kim, Gyoung-Hee;Koh, Young-Jin;Hur, Jae-Seoun;Jung, Jae-Sung
    • The Plant Pathology Journal
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    • v.25 no.3
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    • pp.220-224
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    • 2009
  • The causal agents of bacterial blossom blight in kiwifruit were isolated from flowers displaying symptoms in Korea. The pathogens were characterized by biochemical and physiological tests, and identified on the basis of 16S rDNA and 16S-23S internal transcribed spacer (ITS) sequences. Pathogenicity tests demonstrated that the blossom blight of kiwifruit in Korea is caused by two pathogens, Pseudomonas syringae pv. syringae and P. fluorescens. Carbon source utilization and DNA-DNA hybridization experiments confirmed P. fluorescens as one of the causal agents of blossom blight of kiwifruit. P. syringae pv. syringae and P. fluorescens can be distinguished from each other by the symptoms they produce in flowers. P. syringae pv. syringae primarily affected the stamen, while P. fluorescens caused rotting of all internal tissues of buds or flowers.

Analysis of Direct Normal Insolation Resources in Korea (국내 직달일사량 자원 분석)

  • Jo, Dok-Ki;Yun, Chang-Yeol;Kim, Kwang-Deuk;Kang, Young-Heak
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.130.1-130.1
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    • 2011
  • Since the direct normal insolation is a main factor for designing any solar thermal power system, it is necessary to evaluate its characteristics all over the country. We have begun collecting direct normal insolation data since December 1992 at 16 different locations and considerable effort has been made for constructing a standard value from measured data at each station. KIER(Korea Institute of Energy Research)'s new data will be extensively used by solar thermal concentrating system users or designers as well as by research institutes. From the results, we can conclude that 1) Yearly mean $2.67kWh/m^2/day$ of the all day's direct normal insolation was evaluated for all days all over the 16 areas in Korea. 2) All day's direct normal insolation of spring and summer were $2.91kWh/m^2/day$ and $2.23kWh/m^2/day$, and for fall and winter their values were $2.78kWh/m^2/day$ and $2.77kWh/m^2/day$ respectively. So, spring, fall and winter were higher, and summer was lower than the yearly mean value.

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Quantitative Analysis of Bacillus amyloliquefaciens GR4-5 in Soil (Bacillus amyloliquefaciens GR4-5 균주의 토양 내 정량 분석)

  • Kim, Dayeon;Kim, Byung-Yong;Ahn, Jae-Hyung;Weon, Hang-Yeon;Kim, Sung-Il;Kim, Wan-Gyu;Song, Jaekyeong
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.847-858
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    • 2015
  • Bacillus amyloliquefaciens GR4-5 was isolated from the rhizosphere soil of Korean ginseng and displayed broad-spectrum suppression of ginseng root rot pathogens. The survivability of B. amyloliquefaciens GR4-5 in soil was investigated under three different conditions; indoor, outdoor - of which soil was put in 14 mL tube after treatment - and field environments. Soil samples were collected over a four-week period from three experimental designs, and assessed for 16S rRNA gene copy number by quantitative polymerase chain reaction (qPCR). In outdoor condition, the 16S rRNA gene copy number of Bacillus spp. was 8.35 log copies g $soil^{-1}$ immediately after the GR4-5 treatment. Two weeks later, the 16S rRNA gene copy number of Bacillus spp. (6.70 log copies g $soil^{-1}$) was similar to that of the control (6.38 log copies g $soil^{-1}$). In indoor condition, the 16S rRNA gene copy number of Bacillus spp. maintained in a certain level for a longer period than those in outdoor and field. The 16S rRNA gene copy number of Bacillus spp. in field experiment was reduced faster than that of outdoor condition. Our results show that B. amyloliquefaciens GR4-5 can survive in bulk soil for 1 week, indicating its potential use as a biocontrol agent following 7 day application intervals. This study presents that outdoor microcosm system design could be a useful method to assess easily the survivability of beneficial microorganisms.

Two New Records of Juvenile Oedalechilus labiosus and Ellochelon vaigiensis (Mugiliformes: Mugilidae) from Jeju Island, Korea, as Revealed by Molecular Analysis

  • Kwun, Hyuck Joon;Song, Young Sun;Myoung, Se Hun;Kim, Jin-Koo
    • Fisheries and Aquatic Sciences
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    • v.16 no.2
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    • pp.109-116
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    • 2013
  • Eighteen specimens of juvenile Mugilidae were collected in October 2012 from the southern coastal waters of Jeju Island, and identified based on analysis of their mitochondrial DNA16S rRNA sequences. Seventeen specimens of Oedalechilus labiosus and a single specimen of Ellochelon vaigiensis were found, constituting a new record for these species among Korean ichthyofauna. O. labiosus is identified by the angle at the posterior end of its mouth, which contains a round notch, a darkish dorsal margin of the pectoral fin, the presence of 33-36 lateral line scales, and 23-24 vertebrae. E. vaigiensis is identified by dark dorsal and pectoral fins, the presence of 26 lateral line scales, and 25 vertebrae. The proposed Korean name for Oedalechilus is 'Sol-ip-sung-eo-sok' and that for Ellochelon is 'Nup-jeok-ggo-ri-sung-eo-sok'. The proposed Korean names for the species are 'Sol-ip-sung-eo' and 'Nup-jeok-ggo-ri-sung-eo' for O. labiosus and E. vaigiensis, respectively. We present a key for identification of the Mugilidae family of species from Korea, and include these two newly recorded species.

Experimental investigation of shear connector behaviour in composite beams with metal decking

  • Qureshi, Jawed;Lam, Dennis
    • Steel and Composite Structures
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    • v.35 no.4
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    • pp.475-494
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    • 2020
  • Presented are experimental results from 24 full-scale push test specimens to study the behaviour of composite beams with trapezoidal profiled sheeting laid transverse to the beam axis. The tests use a single-sided horizontal push test setup and are divided into two series. First series contained shear loading only and the second had normal load besides shear load. Four parameters are studied: the effect of wire mesh position and number of its layers, placing a reinforcing bar at the bottom flange of the deck, normal load and its position, and shear stud layout. The results indicate that positioning mesh on top of the deck flange or 30 mm from top of the concrete slab does not affect the stud's strength and ductility. Thus, existing industry practice of locating the mesh at a nominal cover from top of the concrete slab and Eurocode 4 requirement of placing mesh 30 mm below the stud's head are both acceptable. Double mesh layer resulted in 17% increase in stud strength for push tests with single stud per rib. Placing a T16 bar at the bottom of the deck rib did not affect shear stud behaviour. The normal load resulted in 40% and 23% increase in stud strength for single and double studs per rib. Use of studs only in the middle three ribs out of five increased the strength by 23% compared to the layout with studs in first four ribs. Eurocode 4 and Johnson and Yuan equations predicted well the stud strength for single stud/rib tests without normal load, with estimations within 10% of the characteristic experimental load. These equations highly under-estimated the stud capacity, by about 40-50%, for tests with normal load. AISC 360-16 generally over-estimated the stud capacity, except for single stud/rib push tests with normal load. Nellinger equations precisely predicted the stud resistance for push tests with normal load, with ratio of experimental over predicted load as 0.99 and coefficient of variation of about 8%. But, Nellinger method over-estimated the stud capacity by about 20% in push tests with single studs without normal load.

Performance analysis of subjective Loudness meter with ITU-R BS. 1387-1 algorithm for digital audio (디지털 오디오 주관적 음향레벨 계측기 구현을 위한 ITU-R BS. 1387-1의 알고리즘 특성 분석)

  • Ngan, Nguyen Vo Bao;Park, Seonggyoon;Ro, Soonghwan;Han, Chankyu
    • Journal of IKEEE
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    • v.16 no.4
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    • pp.395-404
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    • 2012
  • In this paper, the perceived loudness metering algorithm based on ITU-R BS.1387-1 was investigated and implemented, and its performance was evaluated by applying to 23 pure tones and 9 digital audio samples. Error of the tone test results compared with ISO226:2003 was below 5%, and sample test results, in comparison with Moore's algorithm, showed deviation of less than 4.7% and correlation of 0.96. On the other hand, it was investigated how the implemented algorithm's performance was subject to auditory pitch scale. Its result showed that the algorithm with 37 auditory filters, through correcting a bias effect, has a good performance of less than 2% in comparison with the one with 109 auditory filters.