• Title/Summary/Keyword: 16S and 12S ribosomal DNAs

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Morphological and Molecular Classifications of Genus Pholis

  • Lee, Sung-Hoon;Jang, Yo-Soon;Baik, Chung-Boo;Han, Kyeong-Ho;Myung, Jung-Goo;Lee, Jin-Hee;Choi, Sang-Duk;Kim, Seon-Jae;Kim, Jong-Oh;Hwang, Jae-Ho
    • Animal cells and systems
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    • v.13 no.4
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    • pp.453-460
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    • 2009
  • Morphological and molecular classifications were attempted in an effort to establish species-specific classifications of three species of the genus Pholis in Korea; these species were subjected to morphological and molecular methodologies using body measurements, RFLP, RAPD, and phylogenetic trees using the nucleotide sequences of mitochondrial 16S and 12S ribosomal DNAs, cytochrome c oxidase I, and cytochrome b. The data demonstrated that the three species of genus Pholis are distinct from each other, both morphologically and genetically.

Comparison of Bacterial Communities in Beach Sands along the East Coast of North Gyeongsang Province (경상북도 동해안 해변모래에 서식하는 미생물 군집 비교)

  • Khang, Yongho
    • Korean Journal of Microbiology
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    • v.50 no.4
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    • pp.376-380
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    • 2014
  • Marine beach sands with bacterial pathogens may cause increased outcomes of illness among beachgoers in summer. In this study, pyrosequencing of 16S ribosomal DNAs extracted from 12 beach sands was performed to understand how the environmental factors of wastewaters or human wastes affected the distribution of bacterial communities at the beach of North Gyeongsang province (Yeongdeok and Pohang counties) in the middle of October. It was found that Acidobacteria were dominantly distributed in the sands near the clean seawaters, Proteobacteria in the sands near the river waters, Cyanobacteria in the sands near the wastewaters, and Bacteroidetes in the sands near the beach park. Other phyla groups such as Actinobacteria, Chlorobi, Deferribacteres, Deinococcus-thermus, Firmicutes, Gemmatimonadetes, Nitrospirae, and/or Verrucomicrobia were distributed at low relative abundance (1-5%).

Comparative Cytogenetic Characteristics and Physical Mapping of the 17S and 5S Ribosomal DNAs between Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz.

  • Bang, Kyong-Hwan;Koo, Dal-Hoe;Kim, Hong-Sig;Song, Beom-Heon;Cho, Yong-Gu;Cho, Joon-Hyeong;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.11 no.4
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    • pp.311-315
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    • 2003
  • This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macrocephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A. japonica and A. macrocephala were 2n=24. The length. of the mitotic metaphase chromosomes of A. japonica ranged from $0.70\;to\;1.60{\mu}m$ with a total length. of $12.11{\mu}m$ and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A. macrocephala ranged from $0.90\;to\;2.35{\mu}m$ with a total length of $16.58{\mu}m$ and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A. japonica chromosomes was shorter than that of A. macrocephala, but A. japonica had one subtelocentrics (chromosomes 4) different from A. macrocepha1a. chromosomes. The F1SH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A. macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.