• 제목/요약/키워드: 16S and 12S ribosomal DNAs

검색결과 3건 처리시간 0.02초

Morphological and Molecular Classifications of Genus Pholis

  • Lee, Sung-Hoon;Jang, Yo-Soon;Baik, Chung-Boo;Han, Kyeong-Ho;Myung, Jung-Goo;Lee, Jin-Hee;Choi, Sang-Duk;Kim, Seon-Jae;Kim, Jong-Oh;Hwang, Jae-Ho
    • Animal cells and systems
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    • 제13권4호
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    • pp.453-460
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    • 2009
  • Morphological and molecular classifications were attempted in an effort to establish species-specific classifications of three species of the genus Pholis in Korea; these species were subjected to morphological and molecular methodologies using body measurements, RFLP, RAPD, and phylogenetic trees using the nucleotide sequences of mitochondrial 16S and 12S ribosomal DNAs, cytochrome c oxidase I, and cytochrome b. The data demonstrated that the three species of genus Pholis are distinct from each other, both morphologically and genetically.

경상북도 동해안 해변모래에 서식하는 미생물 군집 비교 (Comparison of Bacterial Communities in Beach Sands along the East Coast of North Gyeongsang Province)

  • 강용호
    • 미생물학회지
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    • 제50권4호
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    • pp.376-380
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    • 2014
  • 경상북도 영덕군과 포항시에 위치한 해수욕장 주변에서 생활하수나 생활쓰레기 등의 환경조건이 해변모래에 서식하는 미생물 분포에 어떤 영향을 미치는지를 조사하기 위하여, 10월 중순에 12곳의 해변모래를 채취하여 16S rRNA 유전자를 pyrosequencing 방법으로 분석하였다. 해수 부근의 청결한 모래에는 Acidobacteria, 담수 부근의 모래에는 Proteobacteria, 생활하수 부근의 모래에는 Cyanobacteria, 해변공원 부근의 모래에는 Bacteroidetes 그룹이 20-90% 정도로 높게 분포하였고, 생활하수가 해수와 합해지는 해변모래에서는 Actinobacteria, Chlorobi, Deferribacteres, Deinococcus-thermus, Firmicutes, Gemmatimonadetes, Nitrospirae, Verrucomicrobia 그룹이 1-5% 정도로 낮게 분포하였다.

Comparative Cytogenetic Characteristics and Physical Mapping of the 17S and 5S Ribosomal DNAs between Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz.

  • Bang, Kyong-Hwan;Koo, Dal-Hoe;Kim, Hong-Sig;Song, Beom-Heon;Cho, Yong-Gu;Cho, Joon-Hyeong;Bang, Jae-Wook
    • 한국약용작물학회지
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    • 제11권4호
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    • pp.311-315
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    • 2003
  • This study was carried out to compare chromosomal characteristics between Atractylodes japonica and A macrocephala. Cytogenetic analysis was conducted based on karyotype analysis and physical mapping using fluorescence in situ hybridization. As a result of karyotype analysis by feulgen staining, somatic chromosome numbers of A. japonica and A. macrocephala were 2n=24. The length. of the mitotic metaphase chromosomes of A. japonica ranged from $0.70\;to\;1.60{\mu}m$ with a total length. of $12.11{\mu}m$ and the homologous chromosome complement comprised six metacentrics, five submetacentrics and one subtelocentrics. On the other hand, the length of the mitotic metaphase chromosomes of A. macrocephala ranged from $0.90\;to\;2.35{\mu}m$ with a total length of $16.58{\mu}m$ and the homologous chromosome complement comprised seven metacentrics and five submetacentrics. The total length of A. japonica chromosomes was shorter than that of A. macrocephala, but A. japonica had one subtelocentrics (chromosomes 4) different from A. macrocepha1a. chromosomes. The F1SH technique using 17S and 5S rDNA was applied to metaphase chromosomes. The signals for 17S rDNA were detected on the telomeric regions of chromosomes 4 and 5 in both A japonica and A. macrocephala. The 5S rDNA signal was found in the short arm of chromosome 1.