• International Journal of Vascular Biomedical Engineering
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• 제1권1호
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• pp.13-23
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• 2003

Backgrounds: The present study in angulated coronary stenosis was to evaluate the influence of velocity and wall shear stress (WSS) on coronary atherosclerosis, the changes of hemodynamic indices following coronary stenting, as well as their effect of evolving in-stent restenosis using human in vivo hemodynamic parameters and computed simulation quantitatively and qualitatively. Methods: Initial and follow-up coronary angiographies in the patients with angulated coronary stenosis were performed (n=80). Optimal coronary stenting in angulated coronary stenosis had two models: < 50 % angle changed(model 1, n=43), > 50% angle changed group (model 2, n=37) according to percent change of vascular angle between pre- and post-intracoronary stenting. Flow-velocity wave obtained from in vivo intracoronary Doppler study data was used for in vitro numerical simulation. Spatial and temporal patterns of velocity vector and recirculation area were drawn throughout the selected segment of coronary models. WSS of pre/post-intracoronary stenting were calculated from three-dimensional computer simulation. Results: Follow-up coronary angiogram demonstrated significant difference in the percent of diameter stenosis between two groups (group 1: $40.3{\pm}30.2$ vs. group 2: $25.5{\pm}22.5%$, p<0.05). Negative WSS area on 3D simulation, which is consistent with re-circulation area of velocity vector, was noted on the inner wall of post-stenotic area before stenting. The negative WSS was disappeared after stenting. High spatial and temporal WSS before stenting fell into within physiologic WSS after stenting. This finding was prominent in Model 2 (p<0.01) Conclusions: The present study suggests that hemodynamic forces exerted by pulsatile coronary circulation termed as WSS might affect on the evolution of atherosclerosis within the angulated vascular curvature. Moreover, geometric change, such as angular difference between pre / post-intracoronary stenting might give proper information of optimal hemodynamic charateristics for vascular repair after stenting.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1242-1248
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• 2005

This study was designed to investigate the in vitro developmental ability and apoptosis of bovine embryos nucleartransferred (NT) with frozen-thawed or cooled donor cells. Cultured adult bovine ear cells were used as donor cells after sub-culturing to confluence (CC), cooling to 4$^{\circ}C$ for 48 h, or freezing-thawing (FT). Apoptotic cells in blastocysts were evaluated for apoptosis by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method. Fusion, cleavage and blastocyst rates were 69.0 (167/242), 68.8 (115/167), and 29.9 (50/167) with CC cells, 70.4 (88/125), 69.3 (61/88), and 29.6 (26/88) with cooled cells and 66.1 (117/177), 70.1 (82/117), and 13.7 (16/117) with FT cells, respectively. Blastocyst rates of NT embryos derived from FT cells were significantly lower than those from CC or cooled cells (p<0.05). In addition, NT blastocysts produced by using FT cells showed significantly higher apoptosis rates (6.4${\pm}$4.0%) than those produced by CC (2.8${\pm}$1.7%) or cooled (2.3${\pm}$1.3%) cells. However, cooling of donor cells had no significant adverse effect on blastocyst rate as well as apoptosis rate. Therefore, our results suggest that cooled cells may be used as an alternative to freshly cultured confluent culture cells, as donor cells, for the production of Somatic nuclear cloned cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.350-353
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• 2005

Effects of sucrose supplement on the pattern of VFA production and microbial protein synthesis in the rumen were examined in sheep consuming basal diet of grass silage (2.5 kg fresh wt/d) that was provided in 24 equal meals each day by an automatic feeder. Four mature wethers were allocated to four experimental treatments in a 4${\times}$4 Latin square design with periods lasting 14 days. The treatments were (1) the basal diet, (2) supplemented with 150 g sucrose and 7.0 g urea, (3) 300 g sucrose and 13 g urea, and (4) 450 g sucrose and 20 g urea given as a continuous intraruminal infusion for 24 h. All infusions were given in 2 litres of aqueous solution per day using a peristaltic pump. The effect of sucrose level on rumen mean pH was significantly linear (p<0.01). There were not significant differences in the concentration of ammonia-N, total VFA and the molar proportions of acetate, propionate and butyrate with the level of sucrose infusion. The molar proportions of isobutyric acid (p<0.05) and isovaleric acid (p<0.001) were significantly reduced when the infused amount of sucrose was increased. The flow of microbial N was linearly (p<0.001) increased with sucrose and urea level. High levels of readily fermentable carbohydrate in a ration reduced the efficiency of microbial protein synthesis in the rumen. It was demonstrated that of the individual fatty acids, only the molar proportion of isovalerate showed a significant negative correlation (R2=$0.3501^{**}$) with the amount of microbial N produced and a significant positive correlation (R2=$0.2735^{**}$) with the efficiency of microbial growth.

• Asian-Australasian Journal of Animal Sciences
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• 제9권1호
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• pp.75-81
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• 1996

The dry matter (DM) disappearance and ruminal solubility of calcium (Ca), phosphorus (P), magnesium (Mg) and potassium (K) in eight Philippine forages were studied. The forages were: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), napiergrass (Pennisetum purpureum Schumach), clopo (Calopogonium mucconoides Desv.), centrocema (Centrocema pubescens Benth.), gliricidia (Gliricidia sepium (Jacq.) Walp.), leucaena (Leucaena leucocephala (Lam.) de Wit.) and sesbania (Sesbania grandiflora (L.) Poir. Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72 h in rumen cannulated sheep. The 0-h bags were washed with deionized water. For the 0-h samples, 20.4, 17.2, 50.7, 52.2 and 80.1% of the DM, Ca, P, Mg and K was solubilized, respectively. At 3-h incubation period, DM disappearance was 10 percentage units higher than that of 0-h incubation whereas mineral disappearance increased by 43, 21, 30 and 13% for Ca, P, Mg and K, respectively. At 72-h incubation period, greater proportion of DM, Ca, especially in P, Mg and K was solubilized with a value of 73.8, 71.5, 85.6, 91.4 and 98.2%, respectively. The average particulate passage rate obtained in the present study was 1.9%/h where as the range of disappearance rates of various mineral elements were : 0.4 to 1.2%/h for Ca, 0.1 to 1.6%/h for P, 0.7 to 2%/h for Mg and 0.1 to 2%/h for K. The effective ruminal solubilization (ERS) of the macrominerals was calculated where particulate passage rate and disappearance rate of the various elements were included in the equation. The ERS of Ca, P, Mg and K was 50.0, 72.6, 83.9 and 94.5%, respectively. Species differences (p<0.05) on the various mineral solubilities were also observed. This study shows that ruminal solubility of macrominerals in selected Philippine forages is K > Mg > P > Ca.

• 대한한방소아과학회지
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• 제14권1호
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• pp.127-136
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• 2000

The frequency of weak children, derived from constitutional and functional factors, has increased recently, but their symptoms have never been observed and analysed. A survey was conducted to evaluate and understand the characteristics of weak children. 311 children who had visited in the weak child clinic of Kyunghee Oriental Medical Hospital from January, 1999 to August, 1999, were the respondents of the Weak Child Questionnaire. They were divided into 4 groups by age and birth weight, and statistical significances were tested by one way analysis of variances and bivariate correlation analysis among groups. The results obtained were as follows: 1. Distribution of sex : male 191 cases, female 120 cases 2. The distribution of age showed the highest in $2{\sim}6$ years(45.3%), followed by $7{\sim}12$ years(31.8%), $0{\sim}1$ years(18.0%), over 13 years(4.8%) in order. There was a statistical difference of all symptoms' mean, except respiratory symptoms, among groups(p<0.01). 3. The distribution of birth weight showed the highest in $2.5{\sim}3.3kg(43.4%)$, followed by $3.3{\sim}4.1kg(39.5%)$, under 2.5kg(5.1%), over 4.lkg(2.1%) in order. There was a statistical difference of digestive symptoms' and total score mean among groups(p<0.01, p<0.05 respectively). 4. The correlation coefficient between general symptoms' mean and other symptoms´ is the highest(p<0.01). From the above results, it was suggested that general symptoms are representative of weak children's characteristics.

• 한국운동역학회지
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• 제16권3호
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• pp.43-51
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• 2006

The purpose of this study was to analyze kinematic variables during turing back kick motion of Taekwondo. The subjects of this study were the 4 skilled and 4 unskilled of male university player in respectively. The experiment of this study was used two 16mm high speed cameras and its speed 125 frames/s. Analysis of this data was three dimensional cinematography using KWON3D program package. The results were as following; 1. In the elapsed time, there was no significance difference statically between a skilled and unskilled group. But skilled group was more fast during the motion of I phase. And unskilled group was more fast during the motion of II phase so called force production section, which had an influence on Diechagi's velocity. 2. In the center of gravity of human body, the changing of it was $1.10{\pm}0.04m$, $1.12{\pm}0.03m$ of LFM(left foot movement) and $1.36{\pm}0.08m$, $1.39{\pm}0.09m$ of RKF(right knee flection), and $1.44{\pm}0.08m$, $1.42{\pm}0.09m$ of RFI(right foot impact). There was no significance difference statically between the two groups. 3. The velocity of heel on impact was 1.13m/s in the skilled group and 1.23m/s in the unskilled group, when each angle of knee was $110.4{\pm}10.9deg/s$, $114.8{\pm}28.4deg/s$. The maximum velocity of each performance was reached before the RKF, and the velocity and angle at impact along by two groups did not show any significant difference statically. 4. In the angular velocity of just RKF of lower leg, there was significance difference statically between the two groups(p<.05).

• 한국가정과교육학회지
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• 제13권3호
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• pp.131-146
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• 2001

The purpose of this study were to develop cooperative learning lesson plan for middle school home economics class and to identify the effect of cooperative learning on students\` self-esteem. The content of lesson was chosen from the food and nutrition section of home economics textbooks for middle school. The main structure of the lesson plan stems from $\boxDr$Lesson Plan Guide of Structuring Cooperative Learning Lesson Plan for Teachers$\boxUl$ by D. Johnson. R Johnson and E. Holubec. Various kinds of references including media reading materials cooperative group activity reports and cooperative group activity checking lists were newly developed according to the content of lesson. Eight hour lesson plans were developed and applied to 2nd grade middle school students and pre-test and post-test were taken to test the effect of Johnson\`s cooperative learning method on students\` self-esteem. Students at a Middle School located in Seoul were divided into two grouts, the three classes as experimental group and the other three classes as control group. The data were analyzed by ANCOVA using SPSS/WIN program. As a result, the hypothesis that the degree of self-esteem of the experimental group is higher than that of control group was adopted (P.(001). Among the sub-factors of self -esteem. the global self-esteem and the social-peer self-esteem scores of the experimental group were higher than that of the control group(p.(001 each). but the school-academic self-esteem score was not different (p> .05) According to the post-experiment free-style report. student as a dynamic subject could get initiatives and interests in home economics class more effectively by cooperative learning. Therefore, it could be said that cooperative learning has positive effect on the promotion of students\` self-esteem and is considered to be a good teaching method of home economics subject.

• 대한치과기공학회지
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• 제34권2호
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• pp.105-111
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• 2012

Purpose: The purpose this study was to evaluate the marginal fitness of metal copings fabricated using rapid prototyping method and to compare the fitness with copings by conventional method. Then clinical availability of fixed dental prostheses (FDPs) fabricated using rapid prototyping method shall be confirmed based on marginal fitness. Methods: Ten same cases of3 unit FDPs epoxy models (abutment teeth 14 and 16) were manufactured. Each of ten epoxy models were scanned by 3shape D-700 scanner to be designed by experienced technician, and photopolymer 3 unit FDPs were fabricated using rapid prototyping methodand fabricated using Lost wax technique (LW) with same models. Marginal fitness of 3 unit FDPs were measured by silicone replica technique. T-test of independent sample for statistical analysis was executed with SPSS 12.0K for Windows. (${\alpha}$=.05) Results: Significantly higher mean (SD) marginal fitness (P<.000) were observed in the RP group $(95.9(18.0){\mu}m$ compared to the conventionally LW group $(80.1(13.8){\mu}m$. Conclusion: Marginal fitness of LW group showed excellent rather than RP group. However marginal fitness of 3 unit FDPs fabricated by RP method did not get out of clinical allowance value range significantly to allow clinical application.

• Asian-Australasian Journal of Animal Sciences
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• 제13권4호
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• pp.474-477
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• 2000

Two experiments were conducted to study the use of urea-treated rice straw (UTRS) and whole sugar cane (WSC) crop as roughage sources for dairy cattle during the dry season. Experiment I, four rumen-fistulated dairy crossbred steers were assigned to receive roughage treatments according to a $4{\times}4$ Latin square design: $T_1=urea-treated$ (5%) rice straw, $T_2=UTRS$ and WSC at 75:25% DM, $T_3=UTRS$ and WSC at 25:75% DM, and $T_4=WSC$. Experiment II, three rumen fistulated, late lactating multiparous Holstein-Friesian crossbreds were randomly allotted to a $3{\times}3$ Latin square design to receive three types of roughages; $T_1=WSC$, $T_2=UTRS$, $T_3=WSC+UTRS$ at 50:50% DM. It was found that combination of UTRS and WSC at 75:25 ratio significantly increased DM intake while intake of WSC alone was lowest. Moreover, inclusion of UTRS into WSC enhanced digestibilites (Exp. I). In Exp. II, combination of UTRS with WSC at 50:50 ratio (DM) enhanced DM intake (kg/d) (p<0.05) and especially milk yield, milk fat and protein percentages. The findings suggest the combined use of WSC and UTRS improved the feeding values of these roughages for dairy cattle during the dry season.

• Asian-Australasian Journal of Animal Sciences
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• 제13권4호
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• pp.457-463
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• 2000

Fifty mid-lactation Holstein cows were used in a six-week feeding trial to study effects of high-forage, high-fat diets on dry matter intake and production performance. Cows were divided into 10 replicates, each consisting of five cows. Each cow was assigned to a control (diet 1) or one of the four experimental diets (high-forage (75%), high-fat (7.5%) (diet 2); high-forage, medium-fat (5%) (diet 3); medium forage (65%), high-fat (diet 4); medium-forage, medium-fat (diet 5)), or a control diet containing about 50% forage and 2% fat. All diets were isonitrogenous (17.7% crude protein). The forage mixture consisted of 20% alfalfa hay, 40% alfalfa haylage, and 40% com silage. Supplemental fat included 80% rumen-protected fat and 20% yellow grease. Dry matter intake was decreased (p<0.01) in cows fed experimental diets (18.4, 20.9, 19.9, and 22.6 kg for cows fed diets 1-4, respectively vs. 27.5 kg for cows fed the control diet). Daily milk production was lower (p<0.05) for cows consuming experimental diets (30.5, 31.3, 31.0, and 32.5 kg for cows fed greater for cows consuming experimental diets (1.74, 1.55, 1.60, and 1.53 kg milk/kg dry matter intake for cows fed diets 1-4, respectively, vs. 1.26 kg milk/kg dry matter intake for cows fed the control diet).