• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.347-355
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• 2006

A study was conducted to predict the rumen microbial protein production based on urinary excretion of purine derivatives in buffaloes fed a diet of wheat straw and concentrate (40:60) at four fixed levels of feed intake. (95, 80, 60 and 40% of preliminary voluntary feed intake) following experimental protocol of IAEA (Phase I). The buffaloes were allocated according to a $4{\times}4$ latin square design. The urinary allantoin, uric acid, total PD excretion (mmol/d) in treatments L-95, L-80, L-60 and L-40 was 20.13, 16.00, 12.96 and 9.17; 1.88, 2.12, 2.11 and 2.15; 22.01, 18.12, 15.07 and 11.32, respectively and were significantly (p<0.05) different among treatments except for uric acid. The rate of PD excretion (mmol/d) was positively correlated with the digestible organic matter intake. Variations were observed in PD and creatinine concentration in spot samples collected at 6-hour interval. However, daily PD:Creatinine ratio (PDC index) appears to be a reasonably good predictor of microbial-N supply. The contribution of basal purine excretion to total excretion of purine derivatives (PD) was determined in pre-fasting period followed by a fasting period of 6 d (Phase II). Daily PD and creatinine excretion (mmol/kg $W^{0.75}$) during fasting averaged 0.117 and 0.456 respectively for buffaloes. The excretion rates of PD decreased significantly (p<0.01) during fasting compare to pre-fasting period, the urinary creatinine excretion remained almost similar. Except for creatinine, plasma concentration of target parameters significantly (p<0.01) declined during fasting. Likewise, glomerular filtration rate (GFR) and renal clearance of allantoin and uric acid also decreased. Based on the PD excretion rates during fasting and at different levels of feed intake obtained in this study, a relationship between daily urinary PD excretion (Y-mmol) and microbial purine absorption (X-mmol) was developed for buffaloes as Y = 0.74X+0.117 kg $W^{0.75}$. The microbial N supply (g/kg DOMI) remained statistically similar irrespective of dietary treatment. The results showed that excretion of urinary purine derivatives is positively correlated with the levels of feed intake in Murrah buffaloes and thus, estimation of urinary purine derivatives and PDC index could be used to determine microbial nitrogen supply when there is large variation in level of feed intake.

• The Journal of Pediatrics of Korean Medicine
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• v.15 no.1
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• pp.117-129
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• 2001

Objective : Hyperlipidemia and hypertension are well recognized risk factors of cerebrovascular disease. So it's very important to evaluate blood pressure and s-cholesterol in adolescence. Material and Methods : April 1999, we were requested health examinaion for high school students of a boys high school and a girls high school in Jeonbuk, Korea. The subjects were 360 students, boys were 317 and girls were 343. They were checked their height, body weight, blood pressure, and s-cholesterol. Results and Conclusion : The mean of systolic blood pressure of study subjects was $111.89{\pm}12.43mmHg$. In boys, it was $116.25{\pm}11.78mmHg$, and in girls, it was $107.87{\pm}11.64mmHg$. The mean of diastolic blood pressure of study subjects was $68.45{\pm}9.40mmHg$. In boys, it was $66.92{\pm}10.00mmHg$, and in girls it was $69.85{\pm}9.40mmHg$. Hypertensives were 18(5.68%) in boys, 10(2.92%) in girls. The mean of s-cholesterol of study subjects was $174.95{\pm}32.28mg/dL$. In boys, it was $178.91{\pm}34.51mg/dL$, and in girls, it was $171.29{\pm}29.66mg/dL$. Hypercholesterolemias were 91(28.71%) in boys, 39(11.37%) in girls. The mean of BMI of study subjects was $21.12{\pm}3.27kg/m2$. In boys, it was $21.05{\pm}3.26kg/m2$, and in girls, it was $21.18{\pm}3.29kg/m2$. Obese boys were 18(5.68%), obese girls were 13(3.79%). The mean of diastolic blood pressure in obese boys was significantly higher than that of normal boys(p=0.001, Mann-Whitney test). The mean of s-cholesterol in obese boys($BMI{\ge}274$) was significantly higher than that of normal boys.(P=0.26, Mann-Whitney test).

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.6
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• pp.1483-1490
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• 2007

This study was carried out to investigate the effects of GCP treatment on the expression of NOS, c-fos, serotonin and substance P in central nerve system of monosodium iodoacetate(MIA)-induced osteoarthritic pain model. Arthritis was induced by injection of MIA(0.5 mg) into knee joint cavities of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was taken distilled water for 20 days. Treated group was taken extracts of GCP by oraly for same duration. Normal group(n=8) was infected with normal saline and was taken distilled water for 20 days. The numbers of NADPH-d positive cells in superficial dorsal horn of spinal cord of treated group($21{\pm}5$) was significantly (p<0.01) decreased compared with control($33{\pm}5$). The numbers of NADPH-d positive cell in dorsolateral periaqueductal gray matter of treated group($111{\pm}16$) was significantly(p<0.01) decreased compared with control($143{\pm}14$). The numbers of c-fos positive cells in dorsal periaqueductal gray matter of treated group($57{\pm}16$) was significantly(p<0.01) decreased compared with control($78{\pm}13$). The numbers of c-fos positive cells in paraventricular thalamic nucleus of treated group($60{\pm}15$) was significantly decreased compared with control($88{\pm}27$). The numbers of serotonin positive cells in median raphe nucleus of treated group($171{\pm}31$) was significantly(p<0.05) decreased compared with control($217{\pm}48$). On the basis of these results, we concluded that GCP treatment has inhibiting effects on the pain transmission in monosodium iodoacetate-induced osteoarthritic pain model in rat.

• Journal of Chest Surgery
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• v.56 no.4
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• pp.244-251
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• 2023

Background: Extracorporeal membrane oxygenation (ECMO) has been widely used in patients with cardiorespiratory failure. The serum albumin level is an important prognostic marker in critically ill patients. We evaluated the efficacy of using pre-ECMO serum albumin levels to predict 30-day mortality in patients with cardiogenic shock (CS) who underwent venoarterial (VA) ECMO. Methods: We reviewed the medical records of 114 adult patients who underwent VA-ECMO between March 2021 and September 2022. The patients were divided into survivors and non-survivors. Clinical data before and during ECMO were compared. Results: Patients' mean age was 67.8±13.6 years, and 36 (31.6%) were female. The proportion of survival to discharge was 48.6% (n=56). Cox regression analysis showed that the pre-ECMO albumin level independently predicted 30-day mortality (hazard ratio, 0.25; 95% confidence interval [CI], 0.11-0.59; p=0.002). The area under the receiver operating characteristic curve of albumin levels (pre-ECMO) was 0.73 (standard error [SE], 0.05; 95% CI, 0.63-0.81; p<0.001; cut-off value=3.4 g/dL). Kaplan-Meier survival analysis showed that the cumulative 30-day mortality was significantly higher in patients with a pre-ECMO albumin level ≤3.4 g/dL than in those with a level >3.4 g/dL (68.9% vs. 23.8%, p<0.001). As the adjusted amount of albumin infused increased, the possibility of 30-day mortality also increased (coefficient=0.140; SE, 0.037; p<0.001). Conclusion: Hypoalbuminemia during ECMO was associated with higher mortality, even with higher amounts of albumin replacement, in patients with CS who underwent VA-ECMO. Further studies are needed to predict the timing of albumin replacement during ECMO.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.3
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• pp.203-208
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• 2007

To develop a new whitening agent for cosmetics from natural products, Pimpinella brachcarpa was selected for its inhibitory effect on melanogenesis in B16 melanoma cells. Crude ethanolic extract of P. brachycarpa and its four fractions-hexane, ethyl acetate(EtOAc), butanol and aqueous were evaluated for antioxidative effects and tyrosinase inhibitory activity. To elucidate the mechanism of active compounds of P. brachycarpa, we investigated the changes in protein level of tyrosinase, TRP-1 and TRP-2 using Western blotting and the changes in mRNA level of tyrosinase using RT-PCR technique. Following UV irradiation, expression of ET-1 in HaCaT keratinocytes was measured by quantitative enzyme immunoassay(EIA) using human ET-1 antibody. Crude ethanolic extract of P. brachycarpa and its four fractions-hexane, EtOAc, butanol and aqueous had free radical scavenging effect by 87.2, 2.5, 97.2, 80.5, 49.8% at 100 ${\mu}g/mL$ and tyrosinase inhibitory effect by 18.3, 15.1, 55.4, 13.1, 0 % at 100 ${\mu}g/mL$. P. brachycarpa EtOAc fraction significantly inhibited melanin production in B16 melanoma cells. Treatment with P. brachycarpa extract for 72 h suppressed the biosynthesis of melanin up to 58 % at 100 ${\mu}g/mL$. Especially, the EtOAc fraction of P. brachycarpa reduced the tyrosinase activity and tyrosinase expression in B16 melanoma cells in a dose-dependent manner. mRNA levels of tyrosinase and TRP-1 were markedly reduced by the EtOAc fraction of P. brachycarpa. Moreover, at the concentrations of $12.5{\sim}50{\mu}g/mL$ of the fraction, the production of UV-induced ET-1 in HaCaT keratinocytes(24 h after 8 $mJ/cm^2$ UVB irradiation) was reduced about 40%(p<0.05). P. brachycarpa could be used as a new natural skin-whitening agent due to the inhibitory effect of on melanin biosynthesis and endothelin-1 expression.

• Korean Journal of Biological Psychiatry
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• v.13 no.4
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• pp.289-298
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• 2006

Objectives : Autism is a complex neurodevelopmental spectrum disorder with a strong genetic component. Previous neurochemical and genetic studies suggested the possible involvement of glutamate N-methyl-D-aspartate(NMDA) receptor in autism. The aim of study was to investigate the association between the NMDA2B receptor gene(GRIN2B) and autism spectrum disorders(ASD) in the Korean population. Methods : The patients with ASD were diagnosed with Autism Diagnostic Interview-Revised and Autism Diagnostic Observation Schedule based on DSM-IV diagnostic classification. The present study was conducted with the detection of four single nucleotide polymorphisms(SNPs) in GRIK2 and family-based association analysis of the single nucleotide polymorphisms in Korean ASD trios using transmission disequilibrium test (TDT). Results : One hundred twenty six patients with ASD and their biological parents were analyzed. 86.5% were male and 85.1% were diagnosed as autistic disorder. The mean age was $71.9{\pm}31.6$ months(range : 26-185 months). We found that rs1805247 showed significantly preferential transmission(TDT ${\chi}^2$=12.8, p<0.001) in ASD. Conclusion : One SNP in GRIN2B gene was significantly associated with ASD in the Korean population. This result suggests the possible involvement of glutamate NMDA receptor gene in the development of ASD.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.29 no.4
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• pp.247-259
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• 1993

The fishing experiment was carried out by the training ship Saebada in order to analyse the mesh selectivity for trawl cod-end, in the Southern Korea Sea and the East China Sea from June. 1991 through August, 1992. The trawl cod-end used in this experiment has the trouser type of cod-end with cover net. and the mesh selectivity was examined for the five kinds of the opening of mesh in its cod-end part. A total of 163 hauls, of which having mesh size 51.2mm ; A 89, 70.2mm ; B 54, 77.6mm ; C 55, 88.0mm ; D 52 and 111.3mm ; E 20 were used respectively. Selection curves and selection parameters were calculated by using a logistic function, S=1/(1+exp super(-(aL+b)) ). The mesh election master curves were estimated by S=1/(1+exp super(-[a(L/M)+$\beta$]) ). and the optimum mesh size were calculated with (L/M) sub(50) of master curve. In these cases 'a' and '$\alpha$' are slope, 'b' and '$\beta$' are intercept. 'L' is body length of the target species of fishes, 'M' is the mesh size, and 'S' denotes mesh selectivity. In this report, the four species of compressed form fishes were taken analized according to fish shape, and 'S' denotes mesh selectivity. In this report, the four species of compressed form fishes were taken analized according to fish shape, and the results obtained are summarized as follows: 1. Red seabream Pagrus major(Temminct et Schlegel) and yellow porgy Dentex tumifrons(Temminct et Schlegel) ; Selection rate in each mesh size of A, B, C, D and E were 99.7%, 97.5%, 91.4%, 76.7% and 57.8% respectively. Selection parameters 'a' and 'b' of mesh sizes C, D and E were 2.65 and -28.62, 4.40 and -77.73, 2.31 and -46.99, and their selection factors were 1.39, 2.10, 1.83 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 3.05 and -5.65 respectively, and (L/M) sub(50) was 1.85. The optimum mesh size of Red seabream was 141mm. 2. Filefish Thamnaconus modestus (Gunther) ; Selection rate in each mesh size of A, B, C, D and E were 99.6%, 98.3%, 91.2%, 80.0% and 48.6% respectively. Selection parameters 'a' and 'b' of mesh sizes C, D and E were 5.82 and -55.10, 2.92 and -36.90, 3.91 and -63.09, and their selection factors were 1.35, 1.44, 1.45 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 3.02 and -4.32 respectively, and (L/M) sub(50) was 1.43. The optimum mesh size was 129mm. 3. Target dory Zeus faber Valenciennes ; Selection rate in each mesh size of A, B, C, D and E were 99.7%, 100%, 83.2%, 91.6% and 65.0% respectively. Selection parameters 'a' and 'b' of mesh sizes C, D and E were 3.85 and -32.46, 4.19 and -57.38, 2.45 and -40.03, and their selection factors were 1.09, 1.56, 1.47 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 2.64 and -3.53 respectively, and (L/M) sub(50) was 1.34. The optimum mesh size was 127mm. 4. Butterfish Psenopsis anomala (Temminct et Schlegel) ; Selection rate in each mesh size of A, B, C, D and E were 99.2%, 34.1%, 46.5%, 14.3% and 2.4% respectively. Selection parameters 'a' and 'b' of mesh sizes B, C and D were 5.35 and -71.70, 5.07 and -69.25, 3.31 and -62.06 and their selection factors were 1.91, 1.75, 2.13 respectively. Selection parameters of master curve '$\alpha$' and '$\beta$' were 3.16 and -6.24 respectively, and (L/M) sub(50) was 1.98. The optimum mesh size was 71mm.

• Microbiology and Biotechnology Letters
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• v.40 no.3
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• pp.220-225
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• 2012

In order to investigate the ethanol fermentation properties of alcohol yeasts a laboratorial strain (CEN.PK2-1D) and two industrial alcohol yeasts (JHS100 and JHS200) of Saccharomyces cerevisiae were cultured in a pure YP medium with 300 g/L glucose and cassava hydrolysate. Spot assay and cell viability tests showed that both the JHS100 and JHS200 strains exhibited higher ethanol tolerance than the CEN.PK2-1D strain. The JHS100 strain demonstrated the highest cell growth, glucose consumption and ethanol production. In particular, an anaerobic batch fermentation of the JHS100 strain using cassava hydrolysate with 250 g/L glucose resulted in a 106.1 g/L ethanol concentration, 0.42 g/g ethanol yield and 3.15 g/L-hr ethanol productivity, which were 53%, 13%, 53% higher than the corresponding values for the CEN.PK2-1D strain. By changing the pure YP medium to cassava hydrolysate, 19% and 17% decreases in ethanol yield and productivity for the CEN.PK2-1D strain were observed, whereas the cultures of the JHS100 and JHS200 stains showed similar ethanol productivities and only an 8% decrease in ethanol yield. Furthermore, the JHS100 and JHS200 stains produced lower levels of glycerol and acetate byproducts than the CEN.PK2-1D strain. Consequently, the outstanding ethanol fermentation performance of the industrial strains might be owing to rapid cell growth, high ethanol tolerance, low nitrogen requirements and the low formation of by-products.

• Food Science of Animal Resources
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• v.33 no.5
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• pp.646-654
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• 2013

In this study, quantitative analysis of major volatile flavor compounds from yogurt was conducted using headspace-solid phase microextraction (HS-SPME) GC-FID analysis technique, and the changes of volatile aroma compounds during the storage period were evaluated. The yogurt was prepared with the addition of 2% cereals, such as, white rice (WR), brown rice (BR), germinated brown rice (GBR) and saccharified germinated brown rice (SGBR). After fermentation, the products were stored at $5^{\circ}C$for 15 d. The major volatile aroma compounds in yogurt, such as acetaldehyde, acetone, diacetyl and acetoin were able to be extracted using HS-SPME technique efficiently. The regression ($r^2$) value of standard curve prepared with various concentrations of individual flavor chemicals was analyzed over 0.9975, and reproducibility was acceptable to apply quantitative analysis. The analysis of volatile components of control sample during storage showed that the acetaldehyde on 0 d was 10.83 ppm, and that contents were increased to 15.67 ppm after 15 d of storage. However, addition of BR, GBR and SGBR decreased the acetaldehyde contents during storage periods. The acetone content of all treatments during storage was not significantly different. The diacetyl content of all treatments were increased during storage and the addition of SGBR showed the highest amount of diacetyl (0.84 ppm) among treatments on 15 d of storage. The acetoin content of yogurt added with grains was higher than that of control during storage. As a result, the content of volatile aroma compounds in yoghurt during storage period could be analyzed HS-SPME extraction technique effectively, and HS-SPME/GC analysis can be considered for quality control of fermented milk products.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.5
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• pp.696-703
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• 2011

We performed a randomized placebo-controlled trial to determine whether or not fermented red ginseng supplementation modulates blood glucose and insulin resistance in type 2 diabetic patients. A total of 38 patients were randomized to either a fermented red ginseng group or placebo group. The patients in the experimental or placebo group consumed 780 mg of fermented red ginseng or cellulose supplement per day for 12 weeks, respectively. Lifestyle factors and dietary intakes of the patients were not altered during the 12-weeks period. In the fermented red ginseng group after 12 weeks, the fasting blood glucose levels were significantly decreased ($136.29{\pm}16.45$ mg/dL to $127.71{\pm}17.74$ mg/dL) and $HbA_1c$ was also decreased. Especially, high HbA1c (HbA1c $\geq$8%, $8.45{\pm}0.56%$ to $7.82{\pm}0.53%$) was significantly decreased compared to low HbA1c (HbA1c <8%, $6.71{\pm}0.85%$ to $6.44{\pm}0.49%$) in the fermented red ginseng group. Serum low-density lipoprotein was slightly decreased in the fermented red ginseng group compared to the placebo group. Homeostasis model assessment-insulin resistance was significantly reduced in the fermented red ginseng group compared to the placebo group. These results suggest that fermented red ginseng supplementation could be helpful to reduce blood glucose by improving insulin resistance in type 2 diabetic patients.