• Journal of Veterinary Science
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• v.24 no.4
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• pp.56.1-56.13
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• 2023

Background: Cold atmospheric plasma is a novel innovative approach for wound care, and it is currently underrepresented in veterinary medicine. Objectives: To investigate the efficacy and safety of using cold atmospheric microwave plasma (CAMP) as an adjunct therapy for wound healing in dogs and cats. Methods: Wound healing outcomes were retrospectively analyzed using clinical records of client-owned dogs and cats who were first managed through standard wound care alone (pre-CAMP period) and subsequently via CAMP therapy (CAMP period). The degree of wound healing was estimated based on wound size and a modified wound scoring system. Results: Of the 27 acute and chronic wounds included in the analysis, 81.48% showed complete healing after the administration of CAMP as an adjunct therapy to standard care. Most wounds achieved complete healing in < 5 weeks. Compared with the pre-CAMP period, the rate of wound healing significantly increased every week in the CAMP period in terms of in wound size (first week, p < 0.001; second week, p = 0.012; third week, p < 0.001) and wound score (first week, p < 0.001; second week, p < 0.001; third week, p = 0.001). No adverse events were noted except for mild discomfort and transient erythema. Conclusions: CAMP is a well-tolerated therapeutic option with immense potential to support the treatment of wounds of diverse etiology in small animal practice. Further research is warranted to establish specific criteria for CAMP treatment according to wound characteristics.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.17
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• pp.7149-7152
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• 2014

A large number of epidemiological studies have demonstrated that obesity is a risk factor for several human cancers. Several animal studies using rodents with diet-induced or genetic obesity have also demonstrated that obesity can promote tumor development. However, the effects of obesity on the early stages of carcinogenesis, and especially on the spontaneous occurrence of somatic gene mutations, remain unclear. To investigate the effects of obesity on the rate of spontaneous gene mutations, we performed reporter gene mutation assays in liver, kidney, and colon, organs in which obesity appears to be associated with cancer development on the basis of epidemiological or animal studies, in mice with high fat diet (HFD)-induced obesity. Six-week-old male and female C57BL/6 gpt delta mice were fed HFD or standard diet (STD) for 13 or 26 weeks. At the end of the experiments, reporter gene mutation assays of liver, kidney, and colon were performed. Final body weights and serum leptin levels of male and female mice fed HFD for 13 or 26 weeks were significantly increased compared with corresponding STD-fed groups. Reporter gene mutation assays of liver, kidney, and colon revealed that there were no significant differences in gpt or $Spi^-$ mutant frequencies between STD- and HFD-fed mice in either the 13-week or 26-week groups. These results indicate that HFD treatment and consequent obesity does not appear to influence the spontaneous occurrence of somatic gene mutations.

• Restorative Dentistry and Endodontics
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• v.13 no.2
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• pp.265-282
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• 1988

The pulp response of posterior composite resins in relation to the thickness of remaining dentin was studied with 120 teeth from 6 dogs, Class V. cavities were prepared on the cervical area of facial surfaces. The thickness of remaining dentin was controlled with Caries Meter$^{(R)}$. The cavities of group A were prepared to show the electrical impedance of 22-26$K{\Omega}$(thickness of remaining dentin:0.4-0.5mm). The cavities of group B, 50-55$K{\Omega}$(thickness of remaining dentin: 0.8-0.9mm). Zinc - Oxide Eugenol cement, Estilux$^{(R)}$ posterior, Heliomolar$^{(R)}$ radiopaque, P-30$^{(R)}$ and Scotchbond$^{(R)}$+P-30$^{(R)}$ were filled in each cavity. After 3days, 1 week, 2 weeks, 4 weeks, 9 weeks and 13 weeks, the teeth and pulp tissue were processed routinely and stained with Hematoxylin and Eosin. Pathological tissue changes were observed with light microscope. The following results were obtained. I. The pulp response of group A cavties was severer than that of group B cavities. 2. In the pulp of group A cavities which were filled with Zinc-Oxide Eugenol Cement, only vascular changes were observed after 3 days and 1 week, severe acute inflammation after 4 weeks, moderate acute inflammation after 9 weeks, and chronic inflammation and formation of granulation tissue after 13 weeks. 3. In the pulp of group A cavities which were filled with Estilux$^{(R)}$ posterior, only vascular changes were observed after 3 days and 1 week. But the inflammatory response has became much severer with the elapsed experimental period. 4. In the pulp of group A cavities which were filled with Heliomolar$^{(R)}$ radiopaque, the inflammatory response with the elapsed experimental period was not severer than that of the pulp of group A cavities which were filled with other materials. 5. In the group B cavities, the difference of pulp response by filling materials was not recognizable. In the group A cavities, the pulp response of Estilux$^{(R)}$ posterior was severest and in order P-30$^{(R)}$, Heliomolar$^{(R)}$ radiopaque was slighter.

• Parasites, Hosts and Diseases
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• v.35 no.3
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• pp.197-202
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• 1997

Diagnosis of early paragonimiasis is difficult because parasitological evidence is not easily obtained. Antibody tests have been proposed as a good substitute for classical diagnostic techniques. Using the crude extracts of Parnsonimus westermnni eggs, metacercariae. 4- and 7-week Juveniles, and 16-week adults as antigens, we observed the early antibody responses. Sera were obtained from 4 experimental cats, fed 50 metacercariae each, at intervals until 13 weeks post-infection. Antibody (IgG) responses were identified by ELISA using extracts of 4-week juveniles, followed by those of 7- and 16-week worms. Antibody responses were minimal against the metacercarial extracts. Antibodies to p. westemoni egg extracts were elevated after 10 weeks post-infection. In immunoblot analysis, more than nine protein bands in 4-week juveniles reacted with the early infection sera. Antigenic proteins in adult worms were different from those of juveniles. After four weeks of infection, 32 and 35 kDa bands in the adult extracts were increasingly reactive. Egg specific proteins at 28, 46 and 94 kDa were reactive only after 10 weeks. Antigenic components reactin료 to the early infection sera changed during the maturation stages of P. westermani; almost all juvenile antigens were replaced by adult antigen components.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2006.11a
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• pp.139-145
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• 2006

Kaneka Corporation (Kaneka) has been manufacturing CoQ10 under GMP regulation since 1977. Kaneka has a sophisticated quality control system and has been supplying high quality CoQ10 materials to the worldwide customers (Kaneka CoQ10) for about 30 years. Kaneka CoQ10 is characterized by a lot of safety data, which are derived from clinical trials with healthy volunteers (single-dose and 4-week multi-dose safety studies), animal studies (13-week sub-chronic study in dogs and 52-week chronic study in rats), three types of mutagenicity test, six type of skin irritation test (for cosmetics), and others. The risk assessment of CoQ10 was performed by Council for Responsible Nutrition (USA). They reviewed many of available clinical data including clinical trials using Kaneka Q10, and concluded that the upper level for supplements (ULS) of CoQ10 is 1,200 mg/day (Hathcock and Shao. 2006, Regulatory Toxicology and Pharmacology, 45, 282 - 288).

• Toxicological Research
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• v.13 no.1_2
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• pp.139-147
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• 1997

Four-week toxicity of EPO(erythropoietin) was investigated using New Zealand White rabbits according to the established regulations of Korean National Institute of Safety Research. Rabbits were administered intravenously seven days per week for 28 days with dosage of 0, 80, 400 and 2000IU/kg B. W./day. Animals administered with EPO showed no significant changes of body weight, water consumption and feed consumption, and no clinical signs and death. They were not significantly different from the control group in hematological and serum biochemical analysis, urinalysis, prothrombin time, and partial thromboplastin time. In this study, we concluded that EPO had no toxic effect in the New Zealand White rabbits when they were administered intravenously below 2000IU/ kg B.W./ day for 28 days.

• 한국약용작물학회:학술대회논문집
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• 2006.11a
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• pp.139-145
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• 2006

Kaneka Corporation (Kaneka) has been manufacturing CoQ10 under GMP regulation since 1977. Kaneka has a sophisticated quality control system and has been supplying high quality CoQ10 materials to the worldwide customers (Kaneka CoQ10) for about 30 years. Kaneka CoQ10 is characterized by a lot of safety data, which are derived from clinical trials with healthy volunteers (single-dose and 4-week multi-dose safety studies), animal studies (13-week sub-chronic study in dogs and 52-week chronic study in rats), three types of mutagenicity test, six type of skin irritation test (for cosmetics), and others. The risk assessment of CoQ10 was performed by Council for Responsible Nutrition (USA). They reviewed many of available clinical data including clinical trials using Kaneka Q10, and concluded that the upper level for supplements (ULS) of CoQ10 is 1,200mg/day (Hathcock and Shao. 2006, Regulatory Toxicology and Pharmacology, $\underline{45}$, 282 - 288).

• Korean Journal of Exercise Nutrition
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• v.13 no.2
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• pp.141-145
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• 2009

The purpose of this study was to investigate the effect of endurance training and Rooibos-tea treatment during 12 week on lipid peroxidation(MDA), oxidative DNA damage(8-hydroxyguanine), and antioxidant enzymes(SOD, GPX) in human. The subjects were divided into three groups; Train+Rooi, Train, and Rooi groups. The Train+Rooi and Rooi group took 3 g of Rooibos-tea for 12 weeks. Blood samples were taken from antecubital vein at before training, after 6week, and after 12 week training. Data were analyzed by two-way ANOVA with repeated measures using the SPSS/PC⁺. The results are summarized as follows: MDA and 8-hydroxyguanine concentration were no significantly differences between group(p>.05). SOD and GPX concentration were significantly increased in Train+Rooi, Rooi group than Train group(p<.05). This results suggested that effects of Rooibos-tea treatment has associated with improve scavenger of antioxidant.

• Korean Journal of Poultry Science
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• v.32 no.2
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• pp.125-133
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• 2005

Morphometric changes in testicular Sertoli and Leydig cells from hatching to adulthood were studied using Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The objective of this study was to understand the developmental phase of the Sertoli and Leydig cells with age. Testis of chickens was fixed by whole body perfusion using a fixative containing $2.5\%$ glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 Um sections stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. The average volume of a testis of 1 week old Korean native chickens was determined as $0.148\;cm^3$ and the parameter increased linearly from 1 week to 21 weeks days $(28.86\;cm^3)$, and did not change from 21 weeks to 64 weeks. The volume density of the seminiferous tubules increased with age from $32.6\%$ at week 1 to $92.89\%$ at week 64. The volume density of the interstitium represents $67.4\%$ of the testicular parenchyma at week 1. This proportion progressively diminished during development to reach a value of $7.11\%$ at week 64. The volume density of the Leydig cells decreased almost linearly from 1 week $(4.9\%)$ to 14 weeks $(1.7\%)$ and remained unchanged thereafter. In contrast, the Sertoli cells occupied a volume density of $3.4\%$ at week 1, increased progressively up to 18 weeks of age $(10.79\%)$ and remained unchanged thereafter. The absolute volume of the Leydig and Sertoli cells per testis increased significantly from week 1 to week 21 but did not change significantly from week 24 to week 64. The number of Leydig cells per testis increased almost linearly from 1 week to 21 weeks, remained high and unchanged with advancing age. The number of Sertoli cells per testis increased gradually with age from 1 week to 14 weeks and remained unchanged thereafter.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.21 no.2
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• pp.141-150
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• 2014

Purpose: This was a study on the expiration date of Ethylene Oxide (EO) gas sterilization and effects of the environmental factors of temperature, humidity and type of cabinet in sterile goods storage area on the expiration date. Methods: Sterile goods storage areas from 13 departments in one hospital were selected and 455 EO gas sterilization samples were prepared and kept in those areas over the 14 months of the study. Each sample was tested with a microbiological culture in the laboratory every week. If the result was positive, the sample was regarded as contaminated. The researcher visited once a month to check the temperature, humidity and type of cabinet. Results: With the exception of 1 sample which was positive at 56th week. 454 samples were confirmed as negative. The environment of the samples storage area was measured monthly. The annual average temperature was $24.2{\pm}1.6^{\circ}C$, and the mean relative humidity $34.7{\pm}15.2%$. The types of cabinet were 7 open and 6 closed. Conclusion: The results of the microbiological culture at 13 months showed that none of the samples were contaminated. Therefore the hospital's existing Expiration Date can be extended from 6 months to 13~14 months.