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젖산균의 Plasmid DNA 분리방법 및 Electroporation에 의한 젖산균의 형질전환에 관한 연구

  • Kim, Seon-Gi
    • 한국유가공학회:학술대회논문집
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    • 1997.05a
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    • pp.41-61
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    • 1997
  • 젖산균의 유전자 연구를 촉진하기 위해 간단하고 신속한 plasmid DNA의 분리방법과 electro-poration을 이용하여 vector plasmid의 간단하고 신속한 전이방법을 얻기 위해 젖산균의 형질전환에 영향하는 요인에 대하여 연구하였으며 연구결과는 다음과 같다. 1. O'Sullivan과 Klaenhammer의 방법을 개선하여 젖산균 plasmid DNA의 분리에 좋은 결과를 얻을 수 있는 신속하고 쉬운 방법을 고안하였으며, genomic DNA 분리에 이용되는 guanidium thio-cyanate 처리방법을 plasmid의 분리에 적용할 수 있었다. 2. L. casei, L. acidophilus. L. delbruekii var. bulgaricus. L. brevis와 L. plantarum 균주에서 plasmid를 확인하였으며, 돼지 분에서 분리된 L. lactis ssp. lactis. L. fermentum과 L. plantarum에서도 plasmid를 분리 확인하였다. 3. Lactococci의 plasmid분리는 lactobacilli와는 달리 mutanolysin의 처리없이도 잘 되었으며, L. lactis ssp. lactis와 Ent. faecalis에서 plasmid를 확인하였다. 4. E. coli plasimd 분리에 이용되는 MPS membrane filter 방법으로 젖산균 plasmid pLZ12의 분리가 가능하였으나, 세포파편이 filter를 막아 사용에 어려움이 있는 것으로 확인되었다. 5. Plasmid 분리없이 electroporation을 이용한 세포 대 세포 전이법으로 간편하고 빠르게 E. coli DH5${\alpha}$에 E. coli Jm109의 plasmid pBX19, pBR322를 전이시켰다. 6. L. lactis ssp. lactis 균주에 lysozyme 처리시 30${\sim}$80%의 생존율을 보였으며, 대부분의 L. acidophilus 균주의 경우 약 70%의 생존율을 보였다. L. casei 102S의 경우는 45분간 처리 시에도 100%의 생존율을 보였다. 8. L. lactis ssp. lactis 균주에 pLZ12를 6.0kV에서 전이시킨 결과 12.5kV에서보다 형질전환 효율이 훨씬 높았으며 lysozyme 처리에 의해 형질전환 효율이 증가되었다. 9. L. acidophilus 균주에 pLZ12를 전이시 6.0kV에서는 전이가 모두 이루어졌으나, 12.5kV에서는 L. acidophilus WIESBY와 NCFM에서 전이가 이루어지지 않았으며, lysozyme 처리 후 pLZ12를 전이시켰을 때 12kV보다 6.0kV에서 형질전환 효율이 증가되었다. 10. Gene Pulser와 Progenitor II를 사용하여 pLZ12를 L. lactis ssp. lactis 균주에 전이하였을 때 Gene Pulser에 비해 Progenitor II의 형질전환 효율이 현저히 떨어졌다. L. acidophilus HY7008과 HY7001은 두 기기 모두 형질전환이 이루어졌으나, L. acidophilus WEISBY와 NCFM은 Progeni-tor II에서 전이가 일어나지 않았으며, Gene Pulser에서 전이균주를 얻어 두 electroporator간에 형질전환 효율의 차이를 보였다. 11. L. casei 102S에 pLZ12를 electroporation시 낮은 전압에서 형질전환 효율이 비교적 좋았으며, 배양 시기를 달리하여 전이시켰을 때 대수생장 말기의 세포가 형질전환 효율이 좋았다. 12. L. casei 102S세포를 각각 10% glycerol, EB, 2차 증류수 등에 녹여 electroporation을 실시하였을 때 각각 $3.8{\times}10^3$, $5.0{\times}10^2$,1.5${\times}10^2$cfu의 형질전환 효율을 보였으며, 1.0mM HEPES, TE buffer를 사용하였을 때에는 전이가 이루어지지 않았다. 13. Plasmid pLZ12의 농도를 달리하여 electroporation을 하였을 때 형질전환 효율이 농도에 비례하여 증가하였다. 14. L. casei 102S에 대수생장 말기의 세포를 채취하여 10% glycerol, 200 Ohms, 25 ${\mu}$FD, 10kV/cm로 plasmid pLZ12를 electroporation할 때 최대 형질전환 효율인 3.8${\times}$10$^{3}$cfu를 얻었으며, lysozyme 처리가 다른 젖산균과는 달리 형질전환 효율을 증가시키지 못하였다. 15. L. casei 102S 세포를 10% glycerol과 EB에 녹여 -20$^{\circ}C$에서 냉동시킨 다음 1일과 7일 후의 세포를 electroporation한 결과 냉동시 세포에 손상을 주는 것으로 인식되었다.

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Growth of Lettuce in Closed-Type Plant Production System as Affected by Light Intensity and Photoperiod under Influence of White LED Light (밀폐형 식물생산시스템에서 백색 LED를 이용한 광도와 광주기에 따른 상추의 생장)

  • Park, Ji Eun;Park, Yoo Gyeong;Jeong, Byoung Ryong;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.22 no.3
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    • pp.228-233
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    • 2013
  • This study was conducted to examine the effect of light intensity and photoperiod of white LEDs as the artificial light source on the growth of leaf lettuce (Lactuca sativa L.) 'Seonhong Jeokchukmyeon' in a closed-type plant production system. Seedlings, transplanted at a density of $20cm{\times}20cm$ in a completely randomized design, were grown under white LEDs (FC Poibe Co. Ltd., Korea), at one of the 3 light intensities (100, 200, or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), and each with one of 3 photoperiods [12/12, 18/6, or 24/0 (Light/Dark)]. Plants were cultured for 22 days under the condition of $21{\pm}2^{\circ}C$, $60{\pm}10%$ RH, and $400{\pm}50{\mu}mol{\cdot}mol^{-1}\;CO_2$. The greatest leaf length and width, fresh and dry weights, and total anthocyanin content were obtained in the 24/0 photoperiod, regardless of the light intensity. Length of the longest root, fresh and dry weights, and number of leaves were greater in light intensity of $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ than 100 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Chlorophyll value was the greatest in the photoperiod 12/12 than 18/6 or 24/0. The results obtained suggest that plant grew the best kept by light intensity at 200 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and photoperiod of 12/12 or 18/6.

A Study on Driver's Perception over the Change of the Headlamp's Illuminance : 3. Driver's psychological property (전조등 조도변동에 대한 운전자의 인식연구 : 3. 운전자의 심리적 특성)

  • Kim, Hyun-Ji;Kim, Hyun-Jin;Kim, Gi-Hoon;Kim, Hoon;An, Ok-Hee
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2006.05a
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    • pp.120-124
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    • 2006
  • In this study, psychological assessment was carried out to investigate the driver's psychological characteristics by the change of the headlight. The participants were 20 men and 20 women in their 20s and thirty-two different conditions in combinations of waveform of light, voltage, and alteration time were used. The questionnaire for the assessment was evaluated by 8 subjective items and 5-point SD criteria of 19 pair's adjective. The results were as follows. 1. The assessment results from SD method indicated 3 factors by factor analysis, and it was shown that A waveform had significances in a sense of security and impetus and B waveform had a significance in a sense of security The levels of the limitations for the voltage change were 12V in the factor of a sense of security and 11V in the factor of a sense of impetus for A waveform, 12.6V in the factor of a sense of security for B waveform. 2. The results of the subjective assessment showed that the limitation of A waveform's brightness change was 12V. Moreover, the limitations of voltage changes were 12.6V for B waveform brightness change, 12V for discomfort, 12.6V for darkness. And the limitation of C waveform's brightness change was 12V.

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A 12b 130MS/s 108mW $1.8mm^2$ 0.18um CMOS ADC for High-Quality Video Systems (고화질 영상 시스템 응용을 위한 12비트 130MS/s 108mW $1.8mm^2$ 0.18um CMOS A/D 변환기)

  • Han, Jae-Yeol;Kim, Young-Ju;Lee, Seung-Hoon
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.3
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    • pp.77-85
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    • 2008
  • This work proposes a 12b 130MS/s 108mW $1.8mm^2$ 0.18um CMOS ADC for high-quality video systems such as TFT-LCD displays and digital TVs requiring simultaneously high resolution, low power, and small size at high speed. The proposed ADC optimizes power consumption and chip area at the target resolution and sampling rate based on a three-step pipeline architecture. The input SHA with gate-bootstrapped sampling switches and a properly controlled trans-conductance ratio of two amplifier stages achieves a high gain and phase margin for 12b input accuracy at the Nyquist frequency. A signal-insensitive 3D-fully symmetric layout reduces a capacitor and device mismatch of two MDACs. The proposed supply- and temperature- insensitive current and voltage references are implemented on chip with a small number of transistors. The prototype ADC in a 0.18um 1P6M CMOS technology demonstrates a measured DNL and INL within 0.69LSB and 2.12LSB, respectively. The ADC shows a maximum SNDR of 53dB and 51dB and a maximum SFDR of 68dB and 66dB at 120MS/s and 130MS/s, respectively. The ADC with an active die area of $1.8mm^2$ consumes 108mW at 130MS/s and 1.8V.

Modeling of Volume Expansion Effects During Infusion of Ringer's Solution (링거액 주입시의 부피팽창 효과에 대한 모델링)

  • Lee, Eun-Ho;Choi, Kyu-Taek;Yeo, Yeong-Koo
    • Journal of Institute of Control, Robotics and Systems
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    • v.12 no.12
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    • pp.1191-1196
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    • 2006
  • In this work the kinetics of volume changes of fluid spaces associated with infusion of Ringer's solution are analyzed using the body fluid space model. During infusion of Ringer's solution, the human body is assumed to be characterized by the fluid space model into which fluid is fed and from which fluid is left. Various infusion types were tested to accommodate different medical situations. Volunteers were given Ringer's solution and the changes in blood hemoglobin were detected. From the comparison with experimental data, the single- and two-fluid space models were found to represent adequately the kinetics of human volume expansion during infusion of Ringer's solution.

CONVOLUTION SUMS OF ODD AND EVEN DIVISOR FUNCTIONS

  • Kim, Daeyeoul
    • Honam Mathematical Journal
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    • v.35 no.3
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    • pp.445-506
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    • 2013
  • Let ${\sigma}_s(N)$ denote the sum of the s-th power of the positive divisors of N and ${\sigma}_{s,r}(N;m)={\sum_{d{\mid}N\\d{\equiv}r\;mod\;m}}\;d^s$ with $N,m,r,s,d{\in}\mathbb{Z}$, $d,s$ > 0 and $r{\geq}0$. In a celebrated paper [33], Ramanuja proved $\sum_{k=1}^{N-1}{\sigma}_1(k){\sigma}_1(N-k)=\frac{5}{12}{\sigma}_3(N)+\frac{1}{12}{\sigma}_1(N)-\frac{6}{12}N{\sigma}_1(N)$ using elementary arguments. The coefficients' relation in this identity ($\frac{5}{12}+\frac{1}{12}-\frac{6}{12}=0$) motivated us to write this article. In this article, we found the convolution sums $\sum_{k<N/m}{\sigma}_{1,i}(dk;2){\sigma}_{1,j}(N-mk;2)$ for odd and even divisor functions with $i,j=0,1$, $m=1,2,4$, and $d{\mid}m$. If N is an odd positive integer, $i,j=0,1$, $m=1,2,4$, $s=0,1,2$, and $d{\mid}m{\mid}2^s$, then there exist $u,a,b,c{\in}\mathbb{Z}$ satisfying $\sum_{k& lt;2^sN/m}{\sigma}_{1,i}(dk;2){\sigma}_{1,j}(2^sN-mk;2)=\frac{1}{u}[a{\sigma}_3(N)+bN{\sigma}_1(N)+c{\sigma}_1(N)]$ with $a+b+c=0$ and ($u,a,b,c$) = 1(Theorem 1.1). We also give an elementary problem (O) and solve special cases of them in (O) (Corollary 3.27).

Effect of Supplemental Lighting in Different Lighting Intensity on Pyruvic Acid and Sugar Content in Onion(Allium cepa L.) (양파재배 시 보광정도에 따른 pyruvic acid와 당 함량)

  • Lee, Eun-Ju;Jeon, Jae-Kyung;Suh, Jun-Kyu
    • Journal of Bio-Environment Control
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    • v.17 no.4
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    • pp.266-272
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    • 2008
  • The aim of this study was to investigate the effect of supplemental lighting with different lighting intensity during growth on the sugar and pyruvic acid content of onion bulbs. As the result of comparison with growth, the content of pyruvic acid and sugar at harvest, supplemental lightening condition showed better growth, lower pyruvic acid content and higher sugar content than control. As to the growth at harvest according to lightening condition, 'Josaeng-ssundeobol' showed better growth as the lightening increased and 'Damrojunggab' had no difference above $12{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PAR. 'Josaeng-ssundeobol' contained much more content of pyruvic acid and sugar than 'Damrojunggab'. 'Josaeng-ssundeobol' had the lowest pyruvic acid content in $24{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PAR and 'Damrojunggab' had no difference above the $12{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PAR. The sugar content of 'Josaeng-ssundeobol' had no big difference above $18{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PAR and that of 'Damrojunggab' had no big difference above $12{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PAR. Desirable indicator to select individuals for the onion breeding is to select individuals that has low pyruvic acid content and high sweetness. Therefore, it will be possible to produce sweet onion conditioned on light supplement over $18{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PAR in 'Josaeng-ssundeobol' and over $12{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PAR in 'Damrojunggab'.

A study on the optimization of a CMP system design for lowering of bull's eye effects (Bull's eye effects 를 줄이기 위한 CMP system의 최적화 설계에 관한 연구)

  • 정병훈;이진구;정철환;이응호;윤형진;백종태;유형주
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.12
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    • pp.72-76
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    • 1996
  • A CMP system is newly proposed and analyzed by a home-made computer progarm. The proposed system is possibly to lower bull's eye effects such that the planarity of a wafer surface using a CMP system can be drasitcally improved. The optimum conditions for a design of the proposed system are verified using the home-made simulation program. For the proposed CMP system with a 12" diameter of platen, the optimum design conditons are the 0.09", 0.10", 0.12", and 0.14", clearance from the platern edge for wafer diameters of 5", 6", 8", and 12", respectively. These clear distance such as 0.09", 0.10", 0.12", and 0.14" are optimum lengths of the sample wafers extended ffrom the platen edge. In other words, shorter orbital lengths result in lowering of Bull's eye effects.

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dsRNA Analysis and Sequence of S12 to Rice dwarf virus Korean Isolate

  • Lee, Bong-Choon;Kwak, Do-Yeon;Hong, Yeon-Kyu;Cho, Hyun-Je;Park, Sung-Tae;Kim, Soon-Chul
    • The Plant Pathology Journal
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    • v.20 no.2
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    • pp.155-157
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    • 2004
  • We isolated Rice dwarf virus (RDV) from infected plants in rice fields (Korea, Japan, China, the Philippines and Nepal) and analyzed their genomic dsRNAs by polyacrylamide gel eletrophoresis. The genomic dsRNAs of the isolates showed distinct electrophoretic mobility profiles. The S12 coding to nonstructural protein of Korean isolate (RDV-Kr) was further analyzed by sequencing. The S12 of RDV-Kr was 1,066bp long and coded for a protein composed of 312 amino acids including three open reading frames of P12, P120Pa and P120Pb. The sequence identities were 96% and 98.6% with Japanese isolates (H, AN), 94.7% with Nepalese isolate (NEL), 94% with Chinese isolate (CK) and the Philippines isolate (P).

Electrical and Optical Properties of Bi12(Si,Ge)O20 Single Crystals (Bi12(Si,Ge)O20 단결정의 전기 및 광학적 특성)

  • Kim, Douk Hoon;Mun, Jung Hak;Lee, Chanku;Lee, Sudae
    • Journal of Korean Ophthalmic Optics Society
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    • v.1 no.2
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    • pp.37-42
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    • 1996
  • The $Bi_{12}(Si,Ge)O_{20}$ single crystals were prepared by Czochralski method and the study of electrical and optical properties were carried out. The activation energy of the electrical conductivity was $E_g$=1.12 eV. The optical energy gap measured in the room temperature is found to be 2.3 eV. A.c. conductivity of crystal $Bi_{12}(Si,Ge)O_{20}$ was measured at temperatures from 290 K to 570 K in the frequency range from 50 kHz to 30 MHz. The a.c. conductivity is proportional to ${\omega}^s$. In view of this it should be hopping conduction mechanisms. At high frequencies, the power exponent was s=2. The low frequency dielectric constants were 54 for $Bi_{12}(Si,Ge)O_{20}$ and 41 for $Bi_{12}(Si,Ge)O_{20}$ single crystals.

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