• Title/Summary/Keyword: 12S

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Temperature and Light Responses on the Growth and Maturation of Gametophytes of Undaria pinnatifida (Harvey) Suringar in Korea (한국산 미역 배우체의 생장과 성숙에 대한 온도 및 광반응)

  • KIM Young Sik;NAM Ki Wan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.505-510
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    • 1997
  • Temperature and light responses on the growth and maturation of gametophytes of Undaria pinnatifida were studied in laboratory culture. The effect of the environmental factors on formation of young sporophyte of U. pinnatifida was also examined in the same culture system. Maximum growth and rapid maturation of the gametophytes were observed at $12:12LD,\;17^{\circ}C$ and 60{\mu}mol\;m^{-2}s^{-1}. However, they survived in a wide range of the examined temperature, light intensity and photoperiod. Particularly they survived under continuous dark condition (0:24LD) until 210 days without any growth and maturation, but died within $10\~40$ days at $30^{\circ}C$. This suggests that optimum rendition for conservation of Undaria gametophytes is under continuous dark photoperiod at $17\~25^{\circ}C$.

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Effects of LED(Light Emitting Diode) Photoperiod and Light Intensity on Growth and Yield of Taraxacum coreanum Nakai in a Plant Factory (식물공장 내 광주기 및 광도가 흰민들레의 생육과 수량에 미치는 영향)

  • Hwang, Yeon Hyeon;Park, Ji Eun;Chang, Young Ho;An, Jae Uk;Yoon, Hae Suk;Hong, Kwang Pyo
    • Journal of Bio-Environment Control
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    • v.25 no.4
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    • pp.232-239
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    • 2016
  • The objective of this study was to examine the effect of photoperiod and light intensity of RBW LED (red:blue:white = 2:1:1) on the growth of Taraxacum coreanum Nakai in a fully artificial light type plant factory. 3 photoperiods and 4 light intensity were used respectively in a fully artificial light type plant production system. Plants were cultured with three photoperiods and four light intensity regimes (conditions) for 270 and 120 days, respectively, using nutrient film technique (NFT) or aeroponics culture methods. For each photoperiod, the total leaves per plant harvested 8 times in all cultivation period was 224 in the 16/8(day/light) photoperiod that had no significant difference from 220 in the 12/12 photoperiod and the lowest number of leaves was 151 occurred in the 8/16 photoperiod, which means that the longer photoperiod, the more leaves harvest. Total fresh weight of above ground was the high in order of in 16/8 photoperiod as 125g, 12/12 photoperiod as 91g, 8/16 photoperiod as 56g. For each light intensity, the total leaves per plant harvested 4 times in all cultivation period was the great in order of $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 123, $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 107, $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 95, $50{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 56 which was the smallest number of total leaves harvest. Total fresh weight of above ground per plant was the high in order of $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 43.6g, $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 34.6g, $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 32.2g, $50{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ as 18.2g. From these results, it was concluded that photoperiod of 16/8 and light intensity at $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ can be used as the light condition of RBW LED (red:blue:white = 2:1:1) for optimal growth of Taraxacum coreanum Nakai in a fully artificial light type plant factory.

Separation of Freon-12 and Air Mixture by Adsorption Process (흡착공정을 이용한 프레온-12와 공기혼합가스의 분리)

  • 강석호;이태진;안희관;김윤갑
    • Journal of Korean Society for Atmospheric Environment
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    • v.9 no.1
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    • pp.101-106
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    • 1993
  • In order to separate the Freon-12 and air mixture$(CF_2Cl_2/Air=0.1/99.9 vol.%)$ by pressure swing adsorption (PSA), the breakthrough curve was experimentally observed in a fixed bed adsorption column. A single adsorber was packed with various adsorbents such as, the activated carbon(S-AC, W-AC) and the molecular sieve(MS-5A, MS-13X). The order of appearance of breakthrough curve is MS-5A > MS-13X > W-AC > S-AC. The activated carbon was found to be more effective adsorbent for separating Freon-12 from the mixture than the molecular sieve was. From the experimental data obtained by the separation of Freon-12 gas out of the air stream in the steady-state PSA process cycle, whose size is the same one of column used for the breakthrough curve observation, it has been confirmed that Freon-rich gas could be obtained from the purge step of PSA and Freon-free air could be obtained from the adsorption step of PSA cycle.

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Retro-synthesis of Analogues of Ginsenosides (역합성법에 의한 진세노사이드 유사체의 합성)

  • Chang, Eun-Ha;Je, Nam-Gyung;Im, Kwang-Sik
    • YAKHAK HOEJI
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    • v.40 no.2
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    • pp.163-169
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    • 1996
  • Glycosidation of 20(S)-protopanaxadiol obtained by the alkaline hydrolysis of total ginsenosides with 2,3,4,6-tetra-O-acetyl-${\alpha$-D-glucopyranosyl bromide in the presence of $CdCO_3$ in benzene-dioxane gave a mixture of acetylated monoglucosides and diglucosides in a total yield of 68%. Under the same condenstion condition, 20-dehydroxyglucosides were formed by dehydration of 12-O-glucosides. The structures of produced glycosides were elucidated as 3-O-${\beta$-D-glucopyranosyl-20(S)-protopanaxadiol, 12-O-${\beta$-D-glucopyranosyl-dammar-20(22), 24-dien-$3{\beta},12{\beta}$-diol, 3,12-di-O-${\beta}$-D-glucopyranosyl-dammar-20(22), 24-dien-$3{\beta},\;12{\beta}$-diol, respectively.

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Inhibition of Calcium Transport by $(1R,9S)-\beta-Hydrastine$ Hydrochloride in PC12 Cells

  • Yin, Shou-Yu;Lee, Myung-Koo
    • Natural Product Sciences
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    • v.12 no.4
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    • pp.217-220
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    • 2006
  • The effects of $(1R,9S)-\beta-hydrastine$ hydrochloride (BHSH) on $Ca^{2+}$ transport in rat pheochromocytoma PC12 cells were investigated. In the presence of external $Ca^{2+}$, BHSH at $100{\mu}M$ inhibited $K^+$ (56mM)-induced dopamine release, and $K^+-induced$ $Ca^{2+}$ influx and a sustained rise of $[Ca^{2+}]_i$. In addition, BHSH at 100 f.!M reduced the sustained rise of $[Ca^{2+}]_i$ elicited by 20 mM caffeine, but not by $1{\mu}M$ thapsigargin, in presence of external $Ca^{2+}$. These results suggest that BHSH inhibited $K^+-induced$ dopamine release and $[Ca^{2+}]_i$ influx, and store-operated $Ca^{2+}$ channels activated by caffeine, but not by thapsigargin, in PC12 cells.

Calculation of the Thermodynamic Properties of R-134a and A Preliminary Study of the Refrigeration Performance (R-134a의 열역학적 물성치 계산과 냉동 성능에 관한 연구)

  • Park, Y.M.;Lee, H.W.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.3 no.4
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    • pp.286-296
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    • 1991
  • The thermodynamic properties of R134a, the prospective R12 alternative, have been computerized using Martin-Hou equation of state and the coefficients given by Willson-Basu. Several experimental results in literatures for PVT data, saturated vapor pressure, saturated liquid density are compared with the calculated results to investigate the accuracy. The average deviation (max. deviation) is 0.13% (0.25%) for saturated liquid density, 0.25% (0.8%) for PVT data. Thermodynamic properties, enthalpy, entropy are compared with the NIST's. The maximum percent difference is 3% for saturated liquid enthalpy, 1.5% for saturated vapor enthalpy, 4% saturated liquid entropy, and 0.7% for saturated vapor entropy. Correction of W-B's coefficients and inclusion of the sixth term of M-H EOS for improvement of accuracy are recommended. R134a and R12 are compared with respect to refrigeration performance. COP's are different from each other within 3%. Refrigeration effect of R134a is superior to that of R12 but refrigeration capacity of R134a is inferior to that of R12 because the volumetric efficiency of the system using R134a is lower than that of the system using R12.

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A Longitudinal Study of the Total Lipid, Total Cholesterol, and Vitamin E Contents and Fatty Acids Composition of Human Milk (수유 기간에 따른 모유의 총지질, 총콜레스테롤 및 비타민 E 함량과 총지방산 조성의 변화에 관한 연구)

  • 문수재
    • Journal of Nutrition and Health
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    • v.26 no.6
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    • pp.758-771
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    • 1993
  • This study was conducted longitudinally following the total lipid and cholesterol content, fatty acids composition and Vitamin E content in human milk from 2-5 days to 12 weeks postpartum of 45 lactating Korean mothers. All samples were from well-defined subjects and uniform collection procedures were used. Total lipid concentration increased from 1.98g/dl at 2-5 days to 3.09g/dl at 12 weeks postpartum. However, there was a large variation in the concentration of total lipids. Total cholesterol concentration tended to decrease from 20.68mg/dl at 2-5days to 17.19mg/dl at 12 weeks. In comparison with transitional and mature milk, human colostrum was characterized by a lower percentage of saturated fatty acids including medium chain length fatty acids and a higher percentage of monounsatureated fatty acids and polyunsaturated fatty acids. Therefore, the P/S and M/S ratios of human milk lipids decreased significanlty during lactation. P/M/S ratio of colostrum changed from 0.38 : 0.84 : 1.00 at 2-5 days to 0.29 : 0.61 : 1.00 at 12 weeks of mature milk. Total tocopherol concentration decreased significantly from 833 ug/dl at 2-5 days to 300 ug/dl at 12 weeks.

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Induction of Neurite Outgrowth by (-)-(7R, 8S)-Dihydrodehyd­rodiconiferyl Alcohol from PC12 Cells

  • Shin Jung Soo;Kim Yu Mi;Hong Seong Su;Kang Ho Sang;Yang Yoo Jung;Lee Don Koo;Hwang Bang Yeon;Ro Jai Seup;Lee Myung Koo
    • Archives of Pharmacal Research
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    • v.28 no.12
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    • pp.1337-1340
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    • 2005
  • A lignan derivative, (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol (DHDA), was isolated from Kalopanax septemlobus L. and was observed to have neuritogenic activity. DHDA at 50 $\mu$M caused a marked induction of neurite outgrowth and an enhancement of nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. However, it did not exhibit any neurotrophic action. At 50 $\mu$M, DHDA enhanced NGF-induced neurite-bearing activity. This activity was partially blocked by the mitogen-activated protein kinase (MAPK) inhibitor PD98059 and by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that DHDA can induce neurite outgrowth and enhance NGF-induced neurite outgrowth from PC12 cells by amplifying up-stream steps such as MAPK and PKC.

Growth and magnetic properties of Tb, Eu, EuTb-substituted garnet single crystal films (Tb, Eu, EuTb가 치환된 가네트 단결정 막의 성장과 자기적 특성)

  • Kim G. Y;Yoon S. G.;Chung I. S;Park S. B;Yoon D. H
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.14 no.5
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    • pp.193-198
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    • 2004
  • Using the $PbO-B_2O_3-Bi_2O_3$ flux system, $(TbBi)_3(FeAIGa)_5O_{12}(TbIG)$, $(EuBi)_3(FeAIGa)_5O_{12}(EuIG)$ and $(EuTbBi)_3(FeAIGa)_5O_{12}(EuTbIG)$ films were grown on $(GdCa)_3(GaMgZr)_5O_{12}(SGGG)$ substrates by the liquid phase epitaxy (LPE). The saturation magnetization of the grown TbIG, EuIG and EuTbIG films was about 150, 950 and 125 Oe, respectively. The TbIG films resulted in the single magnetic domain while the EuIG and EuTbIG films were observed to be the multi magnetic domains by magnetic force microscope (MFM).

Adhesion and Biofilm Formation Abilities of Bacteria Isolated from Dental Unit Waterlines (치과용 유니트 수관에서 분리한 세균의 부착 및 바이오필름 형성 능력)

  • Yoon, Hye Young;Lee, Si Young
    • Journal of dental hygiene science
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    • v.18 no.2
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    • pp.69-75
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    • 2018
  • The purpose of our study is to compare the adhesion and biofilm formation abilities of isolates from water discharged from dental unit waterlines (DUWLs). Bacteria were isolated from a total of 15 DUWLs. Twelve isolates were selected for the experiment. To confirm the adhesion ability of the isolates, each isolate was attached to a glass coverslip using a 12-well plate. Plates were incubated at $26^{\circ}C$ for 7 days, and the degree of adhesion of each isolate was scored. To verify the biofilm formation ability of each isolate, biofilms were allowed to form on a 96-well polystyrene flat-bottom microtiter plate. The biofilm accumulations of all isolates formed at $26^{\circ}C$ for 7 days were identified and compared. A total of 56 strains were isolated from 15 water samples including 12 genera and 31 species. Of the 56 isolates, 12 isolates were selected according to the genus and used in the experiment. Sphingomonas echinoides, Methylobacterium aquaticum, and Cupriavidus pauculus had the highest adhesion ability scores of +3 among 12 isolates. Among these three isolates, the biofilm accumulation of C. pauculus was the highest and that of S. echinoides was the third-most abundant. The lowest biofilm accumulations were identified in Microbacterium testaceum and M. aquaticum. Most isolates with high adhesion ability also exhibited high biofilm formation ability. Analysis of adhesion and biofilm formation of the isolates from DUWLs can provide useful information to understand the mechanism of DUWL biofilm formation and development.