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Genetic Variations of Three Tegillarca granosa Populations Investigated by PCR Technique

  • Yoon, Jong-Man
    • The Korean Journal of Malacology
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    • v.32 no.4
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    • pp.255-261
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    • 2016
  • The selected seven oligonucleotides primers BION-32, BION-33, BION-35, BION-38, BION-40, BION-46 and BION-58 generated the shared loci, specific loci, unique shared loci to each population and shared loci by the three T. granosa populations in Beolgyo, a Chinese site and Wonsan, respectively. The bandsharing value between individuals' no. 03 and no. 04 was 0.816, which was the highest value identified within the Beolgyo population. The primer BION-35 generated the most loci (a total of 70), with an average of 10.0 in the Wonsan population. On average, seven oligonucleotides primers generated 16.1 specific loci in the Beolgyo population, 22.3 in the Chinese population and 39.3 in the Wonsan population. 126 unique shared loci to each population, with an average of 18 per primer, were observed in the Beolgyo population, 63 loci, with an average of 9 per primer, were observed in the Chinese population, and 49 loci, with an average of 7 per primer, and were observed in the Wonsan population. The oligonucleotides primer BION-32 generated 14 unique loci to each population, which were identifying each population in the Beolgyo population. Interestingly, every primer had not distinguished the shared loci by the three populations, major and/or minor fragments of sizes, which were identical in almost all of the samples. As regards average bandsharing value (BS) results, individuals from Beolgyo population ($0.717{\pm}0.057$) exhibited higher BS values than did those from Wonsan population ($0.552{\pm}0.104$) (P < 0.05). The dendrogram resulted from truthful seven oligonucleotides primers, representing three genetic clusters comprising group I (BEOLGYO 01, 02, 03, 04, 05, 06 and 07), group II (CHINESE 08, 09, 10, 11, 12, 13 and 14) and group III (WONSAN 15, 16, 17, 18, 19, 20 and 21). In three T. granosa populations, the longest genetic distance (0.874) displaying significant molecular difference was also between individual no. 02 within the Beolgyo population and individual no. 12 within the Chinese population. Relatively, individuals of the CHINESE population were fairly closely related to those of the WONSAN population.

Studies on Damage, Emergence, and Overwintering of the Chestnut Curculio, Curculio sikkimensis in Chonnam Province (전남지방에 있어서 밤바구미의 피해상황, 우화 및 월동에 관한 연구)

  • Kim Kyu Chin;Kim Chong Pyo
    • Korean journal of applied entomology
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    • v.23 no.2 s.59
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    • pp.132-136
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    • 1984
  • This study was conducted to investigate the bionomics of Curculio sikkimensis in Chonnam province from 1981 to 1983. Damage rates of the chestunt were $26.4\%$ in Damyang, $21.1\%$ in Gwangyang, and $12.9\%$ in Seungju. Adults occurred from the mid-July to the mid-September and showed peak from the mid-August to the early-September. Larvae escaped the chestnut from the early-October to the early-November. Overwintering larvae emerged $3.3\%$ in the first year, $22.78\%$ in the second year and continously overwintered $1.67\%$ in the third year. Larvae showed the highest distribution at $1.67\%$ of soil depth during the overwintering. Mortality of overwintering larvae and course of time showed the linearly correlation. Period of each stage was 5.9 days for egg and 17.17 days for pupa. Adult longevity was 9.10 days for female and 7.03 days for male.

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The Crystal and Molecular Structure of Piperidinothiosemicarbazide (Piperidinothiosemicarbazide의 結晶 및 分子構造)

  • Koo, Chung Hoe;Kim Hoon Sup;Chang Chong Hwan
    • Journal of the Korean Chemical Society
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    • v.19 no.2
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    • pp.85-91
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    • 1975
  • The crystal structure of piperidinothiosemicarbazide, $C_6H_{13}N_3S$, has been determined by single crystal X-ray analysis. The space group is P21/c with four molecules in the unit cell of dimensions $a=14.68{\pm}0.04,\;b=4.59{\pm}0.02,\;c=12.92{\pm}0.04{\AA}\;and\;{\beta}=109.4{\pm}0.2^{\circ}$. Three-dimensional photographic intensities were estimated visually. The structure has been solved by an interpretation of a Patterson synthesis and refined by block-diagonal least-squares methods to give a final R value of 0.14 for 378 observed independent reflections. There are two independent hydrogen bonds in the structure. One of them is of the type N-H${\ldots}$S with the length 3.28 and $3.39{\AA}$, and another is of the type N-H${\ldots}$N with the length $3.03{\AA}$. Apart from the hydrogen bonding system the molecules are held together in the crystal by van der Waals forces.

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Physico-chemical Properties of Bracken(Pteridium aquilinum) Root Starch -1. Morphology and Chemical Properties- 중복기사검색 (고사리(Pteridium aquilinum) 뿌리 전분의 이화학적 특성에 관한 연구 -1. 전분의 일반 성상 및 화학적 특성-)

  • Jo, Jae-Sun
    • Korean Journal of Food Science and Technology
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    • v.10 no.1
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    • pp.57-62
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    • 1978
  • The morphology and chemical properties of bracken (Pteridium aquilinum) root starch were investigated. The starch granules were mainly sphere and cocoon with the diameter of $5-12\mu$. Polarized micrograph indicated that the starch granule had a hilum at the center of granule, showing a crossed-birefringence. X-ray diffraction pattern demonstrated that the granules showed B-type. The density of the starch was 1.49 and the amylose content was 22%. The ferricyanide number and alkali number were 0.292 and 11.03, respectively. Proximate analysis showed that the starch contained 0.52% lipid, 0.63% ash and 150ppm phosphorus of which over 80% were found in the amylopectin fraction. The iodine affinity and molecular weight of amylose were 16.1 and 83,000 respectively. The degree of branching and glucose units per segment of amylopectin were 3.7% and 27, respectively.

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Identification and Genetic Diversity of Korean Tomato Cultivars by RAPD Markers (한국 내 토마토 재재종의 RAPD에 의한 동정과 유전적 다양성)

  • Huh, Man-Kyu;Youn, Sun-Joo;Kang, Sun-Chul
    • Journal of Life Science
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    • v.21 no.1
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    • pp.15-21
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    • 2011
  • Cultivated tomato, Lycopersicum esculentum, is a very important crop. We selected 36 cultivars and studied them for identification and polymorphism by employing random amplified DNA (RAPD) analysis with 80 oligonucleotide primers. Of the 80 primers, 36 primers (45.0%) were polymorphic. Detection of polymorphism in cultivated tomato opens up the possibility of development of its molecular map by judicious selection of genotypes. Molecular markers can also be used for cultivar identification and protection of the plant breeder's intellectual property rights (plant breeders' rights, PBRs). As an example, DNA polymorphism using OPC-13 primer that did not produce the OPC-13-01 band was only found in Junk Pink and Ailsa Craighp cultivars. OPA-12-03 and OPB-15-07 were fragments specific to the TK-70 cultivar and were absent in other cultivars. DNA polymorphism in cultivated tomato in this study was correlated with a type of inflorescence, although some cultivars had exceptions. These approaches will be useful for developing marker-assisted selection tools for genetic enhancement of the tomato plant for desirable traits.

Energy Efficient Alloy Design in PSN-PMN-PZT Ceramic System for Piezoelectric Transformer Application (고효율 압전 트랜스포머용 PSN-PMN-PZT 조성 설계)

  • Choi Yong-Gil;Ur Soon-Chul;Yoon Man-Soon
    • Korean Journal of Materials Research
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    • v.15 no.12
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    • pp.814-817
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    • 2005
  • In order to enhance energy efficiency in high electric conversion devices such as Power transformers, which need to have high power properties, an alloy design approach in $Pb(Zr,Ti)O_3(PZT)$ base ceramic system was attempted $0.03Pb(Sb_{0.5}Nb_{0.5})O_3-0.03Pb(Mn_{1/3}Nb_{2/3})O_3-(0.94-x)PbTiO_3-xPbZrO_3$[PSN-PMN- PZT] ceramics were synthesized by conventional bulk ceramic processing technique. To improve power properties, the various Zr/Ti ratio was varied ]lear their morphotropic phase boundary (MPB) composition of PSN-PMN-PZT system and their effects on subsequent piezoelectric and dielectric properties for the transformer application at high power were systematically investigated using an impedance analyzer. Microstructure and phase information were characterized using X-ray diffractometer (XRD), a scanning electron microscope (SEM) and others. When the Zr/Ti ratio was 0.415/0.465, the value of $Q_m\;and\;k_p$ were shown to reach to the maximum, indicating that this alloy design can be a feasible composition :or high power transformer.

Comparison of Bio-ethanol Productivity Using Food Wastes by Various Culture Modes (에탄올 발효방법에 따른 음식물류 폐기물의 바이오에탄올 생산성 비교)

  • Kang, Hee-Jeong;Li, Hong-Xian;Kim, Yong-Jin;Kim, Seong-Jun
    • KSBB Journal
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    • v.25 no.5
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    • pp.471-477
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    • 2010
  • In order to improve bio-ethanol productivity by various cultivation methods in this paper, the culture modes using food wastes, such as batch culture, high-cell-density fermentation, SSF (simultaneous saccharification and fermentation) by fill & draw, continuous culture by fill & draw were performed and their productivities were compared. SSFs by fill & draw were performed by continuous decompression using 1 L evaporator system, and by 10 L bioreactor without decompression. In addition, the continuous cultures by fill & draw mode using SFW (saccharafied food wastes) medium were performed by changes of 40% culture broth with intervals of 12 h (0.03 $h^{-1}$), 6 h (0.07 $h^{-1}$), 3 h (0.13 $h^{-1}$). Consequently, productivities of bio-ethanol were 2.52 g/L-h and 1.30 g/L-h in batch culture and high- cell-density fermentation, respectively. The productivities of SSF by fill & draw showed 2.24 g/L-h and 2.03 g/L-h in continuous decompression with 1 L evaporator and 10 L bioreactor without decompression, respectively. Also, the productivities in continuous culture by fill & draw modes showed 2.02 g/L-h, 4.07 g/L-h and 6.25 g/L-h by medium change with intervals of 12 h, 6 h, and 3 h, respectively. In conclusion, the highest ethanol productivity was obtained in the continuous culture mode by fill & draw with dilution rate of 0.13 $h^{-1}$.

Motion Vector Predictor selection method for multi-view video coding (다시점 비디오 부호화를 위한 움직임벡터 예측값 선택 방법)

  • Choi, Won-Jun;Suh, Doug-Young;Kim, Kyu-Heon;Park, Gwang-Hoon
    • Journal of Broadcast Engineering
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    • v.12 no.6
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    • pp.565-573
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    • 2007
  • In this paper, we propose a method to select motion vector predictor by considering prediction structure of a multi view content for coding efficiency of multi view coding which is being standardized in JVT. Motion vector of a different tendency is happened while carrying out temporal and view reference prediction of multi-view video coding. Also, due to the phenomena of motion vectors being searched in both temporal and view order, the motion vectors do not agree with each other resulting a decline in coding efficiency. This paper is about how the motion vector predictor are selected with information of prediction structure. By using the proposed method, a compression ratio of the proposed method in multi-view video coding is increased, and finally $0.03{\sim}0.1$ dB PSNR(Peak Signal-to-Noise Ratio) improvement was obtained compared with the case of JMVM 3.6 method.

A Study on Fare Estimation for Demand Responsive Transport (수요응답형교통체계 이용요금 가치 추정 연구)

  • Kim, Wonchul;Namgung, Moon
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.14 no.1
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    • pp.103-111
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    • 2015
  • The purpose of this study is to estimate the appropriate Demand Responsive Transport (DRT) fare for bus users upon introduction of the system, which is to improve ridership on economically non-profitable routes and to conform to the mobility right of those living in presently non-serviced rural areas. For this, a stated preference (SP) survey was adopted to assess respondents' preferences regarding DRT introduction, while the contingent valuation method (CVM) was used to calculate their willingness to pay (WTP) for DRT services. The analysis results show that, compared to the existing bus services with a 1,000 won bus fare, the value of DRT is considered to be 38.85%, 31.03% and 30.12% higher in terms of overall assessment, usability and convenience, respectively. The results of this study are expected to facilitate the decision making aimed at setting the appropriate DRT fare and implementing a DRT system that meets sustainability requirements.

The Effects of Laughter Therapy on Moods, Pain, and Stress of Hospitalized Hospice Patients (웃음요법이 호스피스 입원환자의 기분상태, 통증 및 스트레스에 미치는 효과)

  • Journ, Song-Jah;Lee, Eun-Sook
    • Journal of the Korea Convergence Society
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    • v.10 no.11
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    • pp.481-489
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    • 2019
  • This study was conducted to identify the effect of laughter therapy on the moods, pain and stress of the patients in a hospice ward. Participants were divided as control (23 patients), and experimental group (26 patients). Laughter therapy was provided for 5 days, and 20-30 minutes per day. Data collected on the $3^{rd}$ and $8^{th}$ day, respectively, were analyzed using SPSS win 24.0 program, Chi-square test, fisher's exact test, Independent-test, and Mann-Whitney U test. Experimental groups showed the significant differences in Mood (t= -12.88, p<.001), Pain (t= -6.38, p<.001) and Stress (z= -6.03, p<.001). Conclusion: Laughter therapy was an effective nursing intervention to relieve the negative mood condition, pain and stress on patients, as one of the daily nursing services in the hospice ward.