• Journal of Microbiology and Biotechnology
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• v.28 no.7
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• pp.1147-1155
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• 2018

The degradation efficiency and catabolism pathways of the different methylxanthines (MXs) in isolated caffeine-tolerant strain Pseudomonas putida CT25 were comprehensively studied. The results showed that the degradation efficiency of various MXs varied with the number and position of the methyl groups on the molecule (i.e., xanthine > 7-methylxanthine ${\approx}$ theobromine > caffeine > theophylline > 1-methylxanthine). Multiple MX catabolism pathways coexisted in strain CT25, and a different pathway would be triggered by various MXs. Demethylation dominated in the degradation of N-7-methylated MXs (such as 7-methylxanthine, theobromine, and caffeine), where C-8 oxidation was the major pathway in the catabolism of 1-methylxanthine, whereas demethylation and C-8 oxidation are likely both involved in the degradation of theophylline. Enzymes responsible for MX degradation were located inside the cell. Both cell culture and cell-free enzyme assays revealed that N-1 demethylation might be a rate-limiting step for the catabolism of the MXs. Surprisingly, accumulation of uric acid was observed in a cell-free reaction system, which might be attributed to the lack of activity of uricase, a cytochrome c-coupled membrane integral enzyme.

• Korean Journal of Weed Science
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• v.32 no.1
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• pp.35-43
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• 2012

The objective of this research was to confirm the difference in physiological responses to environmental stresses such as chilling, high temperature, NaCl, and chemical stress (paraquat) in Protox inhibitor resistant-transgenic rice (MX, PX, and AP37) and its non-transgenic counterpart (WT). Transgenic and non-transgenic rice plants were exposed to a chilling temperature of $5^{\circ}C$ for 1 day or a high temperature of $45^{\circ}C$ for 4 days and allowed to recover at $25^{\circ}C$ for 6 days after the chilling treatment or 8 days after the high temperature treatment. Leaf injury, shoot fresh weight, porphyrin biosynthesis substances, and chlorophyll content were investigated in transgenic and non-transgenic rice at 6 days after 0.5% and 1% NaCl treatments or at 5 days after 0~300 ${\mu}M$ paraquat treatments. No significant difference in leaf injury and shoot fresh weight were observed between transgenic and non-transgenic rice during chilling and recovery. Plant height and shoot fresh weight were also similar between transgenic and non-transgenic rice during the high temperature and recovery period (0~5 days). However, plant height and shoot fresh weight in transgenic rice line MX and PX were lower than in non-transgenic rice at 6 days for recovery. Leaf injury, chlorophyll, and Mg-Proto IX ME contents had no significant difference between transgenic rice and non-transgenic rice after NaCl treatment, but Proto IX content for AP37 and shoot fresh weight for PX and AP37 in 0.5% NaCl treatment were significantly reduced compared with non-transgenic rice. There was no difference in leaf injury and shoot fresh weight when comparing transgenic rice and non-transgenic rice after paraquat treatment. Although transgenic rice and non-transgenic rice showed a little difference at a particular measurement period in certain environmental stresses, there was generally no difference in physiological responses between transgenic rice and non-transgenic rice.

• Journal of Ecology and Environment
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• v.35 no.2
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• pp.131-140
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• 2012

There are different types of compost used as soil conditioners and fertilizers. Plants can have different responses to different forms of compost. This field study was performed to examine the effects of different types of compost on growth factors of three dry rangeland species (Atriplex, $Atriplex$ $lentiformis$; Saltwort, $Seidlitzia$ $rosmarinus$; Haloxylon, $Haloxylon$ $persicum$) and soil properties. The experiment was conducted in the Fars Province of Iran during the year 2010-2011. Compost applications consisted of compost tea, solid compost (SC), solid and liquid mixture (MX) and no compost as the control. The study was a factorial experiment based on a randomized complete block design with 3 replications. The results showed that all the tested compost applications enhanced the growth traits of all three species. It was also demonstrated that the use of compost significantly increased the organic matter (1% probability level [PL]), nitrogen concentration (5% PL), phosphorous (5% PL) and potassium (5% PL) concentrations of the soil. The soil's pH level was unchanged (range, 7.3 to 7.6), and the sodium concentration was also significantly decreased (1% PL) by the use of compost. The higher responses were observed in canopy volume and soil sodium and the lower were observed in stem diameter and soil pH level. Among the three plants in the study, Atriplex showed the best response to the application of compost. Based on the results of this study, it can be recommended that the best compost application to increase growth and improve soil condition is the mixed compost (MX) for Atriplex and the SC for Saltwort and Haloxylon.

• Journal of Internet Computing and Services
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• v.19 no.1
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• pp.105-111
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• 2018

This paper presents the analysis of image quality with FHD(Full HD) resolution camera equipped DIC(Digital Image Correlation) platform for the measurement of the architectural structure's relative displacement. DIC platform was designed based on i.MX6 of Freescale. Displacement measurement based on DIC method, the error is affected by image quality factors as pixel number, brightness, contrast, and SNR[dB](Signal to Noise Ratio). The effect were analyzed. The displacement of ROI(Region Of Interest) area within the image was measured by sub-pixel units based on DIC method. The non-contact telemetry property of DIC method, it can be used to long distance non-contact measurement. The various displacement results was measured and analyzed with the image quality factor adjustment according to the distance(25m, 35m, 50m).

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.193-205
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• 2006

This study was to analysis the kinematic and kinetic differences between new walking shoe(NWS : RYN) and general walking shoe(GWS). The subjects for this study were 10 male adults who had the walking pattern of rearfoot shrike with normal foot. The movement of one lower leg was measured using plantar pressure and Vicon Motion Analysis Program(6 MX13 and 2 MX40 cameras : 100 f / s) while the subjects walked at the velocity(1.5m/s. on 2m).. The results of this study was as follows : 1. The NWS was better than the GWS that caused injuries such as adduction, abduction and pronation are reduced While walking on a perpendicular surface, the landing angle and the knees angles were extensive which makes walking more safe which reduces anxiety and uneasiness. 2. The bottom of the NWS were now made into a more circular arch which supports the weight of the body and reduces the irregular angles when wearing GWS. This arch made the supporting area more wide which made the upholding the trunk of the body more effective. The whole bottom of the foot that supports the weight is more flexible in addition, increases the safeness of walking patterns and the momentum of the body. 3. The moment the heel of the foot of the NWS touch the ground, the range of the pressure were partially notable and the range of the pressure on the upper part of the thigh were dispersed The injuries that occurred while walking. primary factors when a shock related injuries are reduced Judgements of the impacts of the knees and the spinal column dispersing could be made.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.3
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• pp.301-306
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• 2009

In this study a 3-component rotating balance, which is designed to measure the thrust (Fz) and two moment components (Mx, My) simultaneously for a rotor test jig, is designed and its performance is validated experimentally. The low voltage signal from the strain gages mounted on the balance is amplified with a rotating amplifier, which is then fed through a slip-ring unit into the data acquisition system. In order to validate the accuracy of the calibration matrix obtained from a static calibration test, an additional reaction type balance is used to measure the thrust from a model rotor simultaneously, and shows very good result. Finally, the expanded uncertainty value, which is obtained from ISO method is estimated to be $2.82\times10^{-1}$, and the balance turns out to be reliable.

• Journal of the Korean Society for Advanced Composite Structures
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• v.1 no.2
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• pp.14-19
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• 2010

Theories for advanced composite structures are too difficult for such design engineers for construction and some simple but accurate enough methods are necessary. The senior author has reported that some laminate orientations have decreasing values of $D_{16}$, $B_{16}$, $D_{26}$ and $B_{26}$ stiffnesses as the ply number increases. For such plates, the fiber orientations given above behave as specially orthotropic plates and simple formulas developed by the senior author. Most of the bridge and building slabs on girders have large aspect ratios. For such cases further simplification is possible by neglecting the effect of the longitudinal moment terms(Mx) on the relevant partial differential equations of equilibrium. In this paper. the influence of the aspect ratio on the natural frequency of the composite laminated plates is studied and it is concluded that the method used is sufficiently accurate for engineering purposes.

• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.674-680
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• 2013

Yeast Dekkera/Brettanomyces bruxellensis is probably the most common contaminant in wineries and ethanol production processes. The considerable economic losses caused by this yeast, but also its ability to produce and tolerate high ethanol concentrations, make it an attractive subject for research with potential for industrial applications. Unfortunately, efforts to understand the biology of D. bruxellensis and facilitate its broader use in industry are hampered by the lack of adequate procedures for delivery of exogenous DNA into this organism. Here we describe the development of transformation protocols (spheroplast transformation, LiAc/PEG method, and electroporation) and report the first genetic transformation of yeast D. bruxellensis. A linear heterologous DNA fragment carrying the kanMX4 sequence was used for transformation, which allowed transformants to be selected on plates containing geneticin. We found the spheroplast transformation method using 1M sorbitol as osmotic stabilizer to be inappropriate because sorbitol strikingly decreases the plating efficiency of both D. bruxellensis spheroplast and intact cells. However, we managed to modify the LiAc/PEG transformation method and electroporation to accommodate D. bruxellensis transformation, achieving efficiencies of 0.6-16 and 10-20 transformants/${\mu}g$ DNA, respectively. The stability of the transformants ranged from 93.6% to 100%. All putative transformants were analyzed by Southern blot using the kanMX4 sequence as a hybridization probe, which confirmed that the transforming DNA fragment had integrated into the genome. The results of the molecular analysis were consistent with the expected illegitimate integration of a heterologous transforming fragment.

• Journal of the Korean Magnetics Society
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• v.5 no.5
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• pp.874-879
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• 1995

Magnetic properties of FeMoN, FeMoTaN, FeTaN and FeTaC films deposited by DC magnetron reactive sputter were investigated, and correlated with their microstructure. FeMoN films were not showen the soft magnetic prop¬erties, because of generated $Fe_{2}Mo$, $Fe_{3-2}N$ and $Fe_{4}N$ phases. Ta added films, however, effectivly retarded the $\alpha$-Fe grain growth and suppressed the generation of Fe nitrides or carbides during heat treatement. The soft magnetic properties of $B_{s}:15\;kG,\;H_{e}:0.25\;Oe,\;\mu':4000(at\;5\;MHz),\;and\;B_s:14.5\;kG,\;He:0.25\;Oe,\;\mu':2700(5MHz)$ were observed in $Fe_{78.8} Ta_{8.5}N_{12.7}\;and\;Fe{75.6}Ta_{8.1}C_{16.3}$ films, respectively.

• Korean Journal of Chemical Engineering
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• v.35 no.11
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• pp.2172-2184
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• 2018

The production of isophthalic acid (IPA) from the oxidation of m-xylene (MX) by air is catalyzed by $H_3PW_{12}O_{40}$ (HPW) loaded on carbon and cobalt. We used $H_2O_2$ solution to oxidize the carbon to improve the catalytic activity of HPW@C catalyst. Experiments reveal that the best carbon sample is obtained by calcining the carbon at $700^{\circ}C$ for 4 h after being impregnated in the 3.75% $H_2O_2$ solution at $40^{\circ}C$ for 7 h. The surface characterization displays that the $H_2O_2$ modification leads to an increase in the acidic groups and a reduction in the basic groups on the carbon surface. The catalytic capability of the HPW@C catalyst depends on its surface chemical characteristics and physical property. The acidic groups play a more important part than the physical property. The MX conversion after 180 min reaction acquired by the HPW@C catalysts prepared from the activated carbon modified in the best condition is 3.81% over that obtained by the HPW@C catalysts prepared from the original carbon. The IPA produced by the former is 46.2% over that produced by the latter.