• Title/Summary/Keyword: 1/4-rate

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Separation of X- and Y-Bearing Spermatozoa III. Separation of bull spermtozoa by Sephadex Gel Filtration (X-정자와 Y-정자의 분이에 관한 연구 III. Sephadex Gel 여과에 의한 우정자의 분이)

  • 이주영;엄기붕;고대환;김종배;정길생
    • Korean Journal of Animal Reproduction
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    • v.12 no.1
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    • pp.24-30
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    • 1988
  • These experiments were carried out to develop new techniques for In Vitro separatin of X-and Y-bearing spermatozoa. The bull semen was applied to the various Gel-Columns filled with swellen Sephadex G-50 Fine and then elutriated wtih Locke solution (elutriation rate; 1ml/3-4min., 1ml/1-2min.). Elutriated solution was fractionated into 1ml by automatic Fraction Collector and spermatozoa included in each fraction were subjected to the estimation of viability and recovery rate, and to B-body test. The results obtained in these experiments were summarized as follows: 1. When the column size and the elutriation rate were adjusted to 15$\times$1.6cm and 1ml/3-4min., respectively, the highest sperm concentration was obtained from the 8th to the 12th fraction. 2. As a trend, the viability of spermatozoa was improved by chromatography, and the degree of improvement ranged 5 to 10 percentage. 3. The average recovery rate of spermatozoa applied to column was 73.2 percentage and ranged 52.6 to 81.3 percentage. 4. The lowest rate of B-body bearing spermatozoa following chromatography was obtained when the column size and the elutriation rate were adjusted to 15$\times$0.8cm and 1ml/1-2min., respectively.

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Response of Amberjacks, Seriola quinqueradiata to the Attracting lamp (유도등에 대한 방어의 반응)

  • 양용림
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.35 no.4
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    • pp.397-403
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    • 1999
  • The author examined the response of Amberjacks, Seriola quinqueradiata [TEMMINCI et SCHLIBEL] to the surface attracting lamps (0.5W, 0.8W, 1W) line in the experimental water tank (550 L$\times$58W$\times$73Hcm).The attracting rate was investigated in accordance with the intervals of lighting and putting out hour (1,5 minute) when each of the attracting lamps was gradually switched off after they were switched on all at once. The results are as follows:1. Total distribution rate of fish in the illuminated section was 92.4% (mean 18.5%) in case of 1 minute interval, and 95.8% (mean 19.2%) in case of 5 minutes interva. 2. Mean distribution rate of fish at the illuminated section: Distribution rate at interval of 1 minute were 19.0% in 1W, 18.4% in 0.5W, and 18.0% in 0.8W respectively. distribution rate at interval of 5 minutes were 19.5% in 1W, 19.2% in 0.5 W, and 18.8% in 0.8W respectively.3. Attracting rates of the last section showed very much increasing as illuminating time elapsed, but there was a little difference of attracting rates according to interval and lighting source. 4. Attracting rate of fish in only last section switched on : Attracting rate at interval in case of 1 minute were 97.0% in 1W, 86.0% in 0.8W and 74.0% in 0.5W respectively. Attracting rate at interval in case of 5 minutes were 97.3% in 1W, 90.0% in 0.5W and 88.0% in 0.8W respectively.

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A Research on the Positive Rate of HBsAg Among the Healthy Korean (한국(韓國)의 건강인(健康人)에서 간염(肝炎) B항원(抗原)(HBsAg) 양성율(陽性率)에 대한 조사연구(調査硏究))

  • Lee, Soo-Young;Kim, Kang-Sueck
    • The Journal of the Korean life insurance medical association
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    • v.1 no.1
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    • pp.133-139
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    • 1984
  • In the 2,218 healthy cases-male 1,574 female 644-who were conducted serum examination from May 1st 1983 to November 1st 1983 by the medical selection of Dae Han Kyouk life insurance Co., the results which investigated the HBsAg by using the Reverse Passive Hemagglutination(RPHA) method are as follows; 1. The number of 167 from 2,218 cases proved to be positive, showing 7.52%, the number of 143 from 1,574 male positive, showing 9.08%, and the number 24 from 644 female positive, showing 3.73%. The positive rate of the male was indicated by 5.36% higher than that of the female as the former marked 2.4 times higher distribution than the latter. 2) The positive rate by the type of occupations appears as follows; The highest positive rate group is that of discharger from military service in 1983, the number of 70 from 736 persons marking 9.51% and the next is the merchant 8.79%(8/91), employee of a company markes 8.53%(44/516), high school boy 7.69%(19/247), house wife 5.71%(10/210), carrier woman 3.45%(10/290) and the group of the high school girl records the minimum rate of all, 3.13%(4/128). 3. The positive rate by the age group appears as follows; The highest rate group is that of 31 to 35 years, marking 9.80% positive rate, and the next is $26{\sim}30$(9.3%), $21{\sim}25$(8.01%), $41{\sim}45$(6.96%), $36{\sim}40$(6.9%), those below 20(5.99%), 46 to 50 years shows the minimum rate of all(2.94%) Among the male case, 26 to 30 years shows the highest positive rate by 10.59% and female, 31 to 35 years shows the highest rate by 7.89%. 4. The positive rate by the blood type represents as follows; Blood type A shows 7.30%, type B 7.17%, type O 6.11% and type AB indicates 5.88% in order.

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Kinetics Study of the Reaction of Bromine with Phenylhydrazine in Sulfuric Acid Media (페닐히드라진과 브롬의 반응메카니즘에 관한 연구)

  • Park Byoung Bin;Park Il H.;Kong Young Kun;Choi Q. Won
    • Journal of the Korean Chemical Society
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    • v.21 no.4
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    • pp.227-234
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    • 1977
  • The reaction of phenylhydrazine with bromine in sulfuric acid solution has been studied kinetically. The pseudo-second-order rate constant is approximately inversely proportional to hydrogen-ion concentration when the concentration of sulfuric acid is lower than 1M. arom the study of the effect of potassium bromide concentration on the rate constant, it is concluded that both neutral bromine and tribromide ion participate in the reaction, the rate constants in 0.01M $H_2SO_4$ being $5{\times}10^5M^{-1},sec^{-1}\;and\;0. 7{\times}10^5M^{-1},sec^{-1}$, respectively at $20^{\circ}C$. The pseudo-second-order rate constant of 2.4-dinitrophenylhydrazine-bromine reaction is independent of hydrogen ion concentration. From the KBr addition experiment, the rate constants for $Br_2\;and\;Br_3^-$ were obtained as $1.2{\times}10^5M^{-1},sec^{-1}\;and\;2.0{\times}10^4M^{-1},sec^{-1}$, respectively.

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Effect of Paclobutrazol on Growth, and High Temperature and Drought Stress in Perennial Ryegrass (Paclobutrazol 처리가 Perennial Ryegrass의 생육 및 고온과 건조 Stress에 미치는 영향)

  • 김태일;구자형;원동찬
    • Asian Journal of Turfgrass Science
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    • v.3 no.1
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    • pp.24-33
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    • 1989
  • This study was conducted to investigate the effect of paclobutrazol [(2 RS , 3 RS )1-(4- chlor-ophenyl )-4, 4- dimethyl -2- (1, 2, 4- triazol -1- yl )- pentan -3-01] on the tolerance of hi-gh temperature and drought stress as related to growth retardation , iranspiration rate , soil water content , nitrogen level and photosynthetic rate in perennial ryegrass ( Loliurn perenne L . ' Omega H , ). Plants were given a 30 ml soil drench of paclohutrazol at the concentrations of 0, 0.01, 0.1, 1.0, 10.. 0, mg / 6 .5cm- diameter pot . The rcsults were as follows : 1. Increasing concentrations of paclohutrazul reduced plant height , leaf area , fresh weight and dry weight , hut increased chlorophyll content per unit area . The number of tillers and leaf width were not affected hy the paclobutrazol concentrations . 2. The proper concentration of paclohatrazol on growth retardation in perennial ryegrass was about I mq /pot , hut leaf deformity and severe growth retardation were shown at high concentration of 10 mq / pot . 3. Perennial ryegrasses grown at 30˚C were shown significantly short plant height and low leaf nitrogen level compared with those grown at 20˚C. Increasing concentrations of paclohutrazol at 20˚C increased nitrogen level hut it could not increase nitrogen level at 30˚C . 4. During the drought stress , increasing temperatures significantly promoted transpiration rate and wilting time . It took about 5 days at 20˚C and 3 days at 30˚C to reach wilting time of leaves from water stress treatment . Soil water contents at wilting time of non-treated controls were averaged 6. 871% at 20˚C and 6. 17% at 30˚C 5. Paclohutrazol reduced transpiration rate at high temperature and drought stress . Wilting appeared at the lower water content of soil according to increasing concentrations of paclobutrazol at 30˚C hut there were no differences among concentrations of at 20˚C. 6.Paclohutrazol treatment at 1 rag /pot reduced injury rate of leaves from 67.1 % and 100 % in control plants to 15.7% and 80% at 20˚C and 3010, respectively. 7. Photosynthetic rate per unit area was significantly reduced at high temperature . Paclohutrazol stimulated photosynthetic rate with increase of concentrations at 20˚C but there was no increasing effect at 30˚C.

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Evaluation of Terrestrial Gamma Radiation and Dose Rate of the Ogcheon Group Area (옥천층군 일대의 지표방사능과 감마선량 평가)

  • Yun, Uk;Cho, Byong-Wook
    • The Journal of Engineering Geology
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    • v.30 no.4
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    • pp.577-588
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    • 2020
  • We evaluated the distributions of primordial radionuclides and effective dose rate of the Ogcheon Group, which includes rocks with high uranium content. Terrestrial gamma radiation was measured at 421 points using a portable gamma ray spectrometer. Dividing the study area into five geological units (og1, og2, og3, og4, and igneous rocks) revealed no significant difference in the concentration of surface radioactivity among the types. The concentrations of 40K, eU, and eTh for all samples ranged from 0.7% to 10.3% (average 5.2%), 0.6 to 287.0 ppm (average 8.5 ppm), and 4.0 to 102.4 ppm (average 31.3 ppm), respectively. The absorbed dose rate in the study area (calculated from the activity concentrations of 40K, eU, and eTh) was in the range of 28.84 to 1,714.5 nGy/h (average 195.4 nGy/h). Among the five geological units, the lowest average was 166.3 nGy/h (for og1) and the highest average was 233.3 nGy/h (for og2; median 198.1 nGy/h). The outdoor effective dose rate for the area obtained from the absorbed dose rate was in the range of 0.04 to 2.10 mSv/y (average 0.24 mSv/y). Except for the four sites located in the uranium-bearing coal bed of og2, none of the studied sites exceeded 1 mSv/y.

Corrosion Behaviour of Water Pipes (수도관의 부기거동에 관한 연구)

  • 김원만;박영식
    • Journal of environmental and Sanitary engineering
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    • v.7 no.1
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    • pp.1-16
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    • 1992
  • Corrosion of pipes Is one of the most serious problems in the maintenance of water worlds. Corrosion is promoted not only by physical factors such as temperature, but also by electrochemical factors including concentration of soluble metal ions, chlorine ion, pH, DO and microorganisms. Corrosion products also affect corrosion rate. In this research, study results are summarized as follows ; 1) Corrosion test was performed for 4 weeks at $70^{\circ}C$, pH 7.0 with specimens of 4 types of metal materials used as service pipes. Corrosion rate and S.E.M were analyzed. The results were showed that corrosion 1.ate of carbon steel pipe was 4.10~5.22 $mg/\textrm{cm}^2$ . week, galvanized steel pipe 0.98~1.34$mg/\textrm{cm}^2$. week, Copper pipe 0.02~0.04$mg/\textrm{cm}^2$. week, stainless steel pipe 0.05~ 0.10$mg/\textrm{cm}^2$ . week. 2) When corrosion rate was tested for tile types of pipes at pH 7 and both $25^{\circ}C$ and $75^{\circ}C$, avaerage corrosion rate for 6 weeks at $25^{\circ}C$ Ivas 2.26$mg/\textrm{cm}^2$ . week in carbon steel pipe, 1.99$mg/\textrm{cm}^2$. week in galvanized steel pipe, 0.26 $mg/\textrm{cm}^2$. week in stainless steel pipe. At $87^{\circ}C$, average corrosion rate for 4 weeks u.3s 4.56 $mg/\textrm{cm}^2$. week in carbon steel pipe,

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Study on the Developmental Rate of In Vitro Cultured Cats Oocytes Recovered from Ovaries Collected at Different Stages of the Reproductive Cycle (번식주기의 단계별로 회수한 고양이 난자의 체외수정과 체외발생에 관한 연구)

  • 박상훈;이명헌;김무강;김상근
    • Journal of Embryo Transfer
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    • v.18 no.2
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    • pp.157-161
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    • 2003
  • The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes in vitro maturation of cats oocytes and development of IVM/IVF embryos. The results were summarized as follows : 1. The fertilization and developmental rate of fresh and salts-stored oocytes with and whithout cumulus cells were 65.7%, 17.1% and 28.6%, 8.6% and 57.1%, 13.3%, 23.3%, 3.3%, respectively. The rate of oocytes with cumulus cells(13.3%∼65.7%) was higher than that of denuded oocytes(3.3%∼28.6%). 2. The fertilization and developmental rate of oocytes recovered from ovaries collected at different stages of the reproductive cycle were 68.9%, 44.4%, 48.9% and 17.8%, 8.9%, 12.8%, respectively. 3. The fertilization and developmental rate of oocytes in vitro cultured at different time of incubation(24, 36 and 48 h) were 66.7%, 46.7%, 48.9% and 17.8%, 11.1%, 8.5%, respectively. respectively. The rate of oocytes incubated 24 h(66.7%) was higher than that oocytes incubated 36 and 48 h(46.7%∼48.9%). 4. The fertilization and developmental rate of oocytes treated activation and non-activation oocytes were 57.4%, 31.4% and 22.9%, 11.4%, respectively. The rate of oocytes treated activation was higher than that oocyte treat non-activation.

Envrionment-Friendly Effects of Espil and Copper Hydroxide for Prevention of Powdery Mildew on Cucumber, Tomato, and Red Pepper (에스필과 수산화동 혼용에 의한 오이, 토마토, 고추 흰가루병의 친환경 방제 효과)

  • Soh, Jae-Woo;Han, Kyung-Sook;Lee, Seong-Chan;Lee, Jung-Sup;Park, Jong-Han
    • Research in Plant Disease
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    • v.20 no.2
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    • pp.95-100
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    • 2014
  • This research was performed to examine the effects of mixed espil and copper hydroxide for powdery mildew prevention on cucumber, tomato and pepper. On prevention effect for powdery mildew on cucumber, results revealed that mixed espil and copper hydroxide with a ratio of 4 : 1, disease incidence rate was 18.9% and prevention effect was 69.9%. Another treatment with a ratio of 8 : 1 showed an disease incidence rate of 18.1% and prevention effect of 71.1%, thus, showed great effectiveness. For powdery mildew on tomato, espil and copper hydroxide were mixed using the ratio 4 : 1, results showed an disease incidence rate of 12.4% and prevention effect of 85.3%. Treatment using the ratio of 8 : 1, results showed an disease incidence rate of 14.3% and prevention effect of 83.0%, thus, showed great effectiveness. For powdery mildew on redpepper, espil and copper hydroxide were mixed using the ratio of 4 : 1 with results showed disease incidence rate of 17.7% and prevention effect of 83.0%. From the results, this treatment is the most effective with the lowest attack rate and highest prevention effect. Deducing from the study, it was found out that using mixed espil and copper hydroxide using the ratios 4 : 1 or 8 : 1 are the most effective method for powdery mildew prevention. Mixed ratio of 4 : 1 or 8 : 1 was most effective for preventing powdery mildew on cucumber and tomato, while the espil and copper hydroxide ratio of 4 : 1 was the most effective method for powdery mildew prevention on pepper.

Determination of Transferring Period of Several Plants using Image Processing (영상처리를 이용한 작물의 모종시기 결정)

  • 민병로;김웅;김동우;이대원
    • Journal of Bio-Environment Control
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    • v.13 no.3
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    • pp.178-184
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    • 2004
  • This study carried out to develope the vision system which automatically finds out a optimum transferring period of plants (Perilla, Platycodon grandifloums and Lactuca sativa) by using image process-ing. This system mearsured a height, long diameter and short diameter of the three plants with 20 replications. Following results were obtained on each plant. Compared with real data to be measured by hand with the vernier calipers, height, long diameter and short diameter of Perilla showed 0.5 mm average error rate with 1.7%, 4.7 mm average error rate with 3.9% and 5.5 mm average error rate with 6.9% respectively. Those of Platycodon grandifloums showed 2.4 mm with 8.1%, 3.4 mm with 7.2% and 4.0 mm with 10.4% respectively. Those of Lactuca sativa showed 4.0 mm with 9.1 %,3.4 mm with 7.2% and 3.6 mm with 9.4% respectively. The system could be used to transfer accurately the plant seedling, if the system were improved enough to reduce error rate for the optimum transferring period of a plant in the greenhouse.