The study was conducted to obtain the basic information on increasing aromatic degrees by shading and organic matter application on Condonopsis lanceolata. The result were as followings : Fresh root wt. in shading 55 % treatment was increased about twices, whereas fresh root weight in non-shading was decreased. But growth of ground parts such as vine length, leaf width, and leaf number was promoted under non-shading treatment. Macroelements such as K, Ca, and Mg, were increased in non-shading and higher organic matter application. The components such as crude protein, fiber, and ash were increased in the shading 55% treatment compared with non-shading. In the sixteen amino acids, arginine contents were the highest in non-shading and organic matter 30M/T. Recovery yield of essential oil was 0.005% in non-shading. The highest was organic matter 30M/T in the non-shading as 0.007%. Major aromatic constituents were 11 kinds of aliphatic alcohols such as I-hexanol, cis-3-hexenol, and trans-2-hexanal. As a result, major aromatic degree was higher in shading 55%, also increased in the much organic matter application. It was considered to be applied dense shading 55% and much organic matters to produce Condonopsis lanceolata plants which have high aroma and good quality.
Sixty-seven Hanwoo calves were allocated into two groups, 27 spring calves (SC) and 40 fall calves (FC) to investigate the effects of calving season on water and nutrient intakes, growth performance and disease occurrence for the nursing and the post-weaning period. Mean DMI of 3 to 4 mo old FC was lower than that of SC due to low temperature in winter for FC. Water intake was higher for FC (birth to 1 mo old) but lower for SC (2 to 4 mo old) than the others. Mean BW did not differ between SC and FC, but average daily gain for over 2 mo old SC were significantly higher than FC. Diarrhea was mainly observed when the calves were before 60 d old; in particular, it occurred more frequently for less than 30 d old FC compared with SC. The present results indicate that calving season may affect water intake, feed intake and growth performance of Hanwoo calves.
Cyhalofop/bentazon ME and pyribenzoxim EC herbicides were originally developed as foliarapplied herbicides to control weeds at 4-5 leaf stages of barnyard grass 20-25 days after seeding (DAS) in direct-seeding culture but further possible utilization of these two herbicides earlier than 3-4 leaf stages of rice were evaluated for a field where early weed infestations might be severe. The application of cyhalofop/bentazon ME and pyribenzoxim EC at right after rice emergence and the 2-3 leaf stages of rice had an excellent weed control efficacy with above 90% up to 30 DAS without a phytotoxicity of rice plant and the control efficacy of over 80% was maintained until 60 DAS. However, these two herbicides controlled Echinochloa crus-galli very effectively above 97% but Aneilema keisak and Aeschynomene indica were not controlled by cyhalofop/bentazon ME and Cyperus serotinus by pyribenzoxim EC. Therefore, to control those problem weeds, second systematic application of pyribenzoxim EC and pyrazosulfuron/mefenacet G for A. keisak and A. indica, and pyrazosulfuron/molinate G, cyhalofap/ azimsulfuron/molinate G, pyrazosulfuron/mefenacet G and bentazon SL for C. serotinus at 30 DAS was found to be very efficient herbicide systems.
Hydroxyproline and Pro-Hyp dipeptide are the digestive products of collagen hydrolysate called collagen peptide. Some suggested that collagen peptides could improve aged or damaged skins, however, the effects of collagen peptides on the skin have not been known. In this study, we investigated the effects of digestive products of collagen peptides, hydroxyproline and Pro-Hyp dipeptide on skin quality using the UV-damaged dorsal skin of hairless mouse as a model system. Female SKH hairless mice were pre-irradiated with UV for 7 weeks, and then hydroxyproline, Pro-Hyp dipeptide were orally administered for 7 weeks with UV irradiation. Wrinkle formation (by replica image), skin elasticity, barrier status (by TEWL, transepidermal water loss), epidermis thickness, and biophysical changes in the stratum comeum (by hematoxylin & eosin staining) were examined. With the oral peptide treatment, effects such as skin barrier maintenance, anti-skin thickening, and recovery of the stratum corneum were observed. These results indicate that oral intake of collagen peptides may have beneficial effects on damaged skin cells.
Lee, Hyo-Won;Lee, Hyo-Jin;Kim, Won Ho;Yoon, Bong Ki;Ko, Han Jong
Journal of The Korean Society of Grassland and Forage Science
/
v.36
no.4
/
pp.318-324
/
2016
In order to study the effect of barley, Italian ryegrass (IRG), and legume mixture on nitrogen fixation and transfer to grasses on spring paddy field, an experiment was carried out from Oct. 2006 to June 2007 in Naju, Korea. A split plot design with three replications was used for the experiment. One reference plot was assigned for each treatment to determine nitrogen fixation. Main plots consisted of Chinese milk vetch, crimson clover, forage pea, and hairy vetch with barley, respectively. Subplot treatment were barley or IRG with four seeding ratio of legumes (50:50, 60:40, 70:30, and 80:20). To estimate N fixation by legumes, $^{15}N$ isotope dilution technique was used. $^{15}N$ fertilizer [$(^{15}NH_4)_2SO_4$ solution at 99.8 atom N] was uniformly applied to $600cm^2$ in the middle of each plot on April 15, 2007. Plots were harvest by hand on June 8, 2007. Dried sample were ground to a fine power and analyzed for total N isotope N. $^{15}N$ was determined using elemental analyzer-isotope ratio mass spectrometry. The calculation of N transfer was determined with the isotope dilution method. The content of N was higher in legumes than that in barley or Italian ryegrass. Nitrogen level in forage pea was significantly higher than that of other legumes. There were significantly differences in N content between legumes in IRG mixture. Atom % $^{15}N$ excess was significantly different in legumes with barley. The 60:40 sub plot had higher (p<0.05) atom % $^{15}N$ than other seeding ratio treatments. The enrichment ranged from 0 to 0.58. Compared to barley, the enrichment of IRG with its accompanied legumes was higher, ranging from 0.38 to 1.0. The N derived from the atmosphere (Ndfa) ranged from 0% to 49.5% with barley-legume mixture. It ranged from 0 to 60.5% in IRG-legume plots. N transfer from legumes to neighboring grasses was 12.3 to 90.9 kg/ha for barley-legume mixture and 31.7 to 107.8 kg/ha for IRG plots. IRG plots showed higher N transfer for IRG-legume mixture in general based on difference method. Based on $^{15}N$ dilution method, the N transfer was 0 to 36.1 kg/ha for barley-legume mixture and 0 to 50.6 kg/ha for IRG plots. There was a tendency toward higher N transfer on the difference method than that of the $^{15}N$ dilution method.
Journal of The Korean Society of Grassland and Forage Science
/
v.6
no.1
/
pp.31-37
/
1986
In order to study the possibility of grassland improvement by spring sowing in the forest, microenvironmental conditions, emergence, percentage of grasses and weeds, root weight and dry matter yield of grasses were investigated. Two field sites (forest grassland and full-sunlight grassland) and two sowing times (March 20 and April 10) were assigned. The condition of the forest grassland was area of pine trees with 50% shading, and the experiment was performed at the Livestock Experiment Station in Suweon, 1984. The results obtained are summarized as follows: 1. For germination and early growth of grasses, full-sunlight grassland was more advantageous than forest grassland. Growth after that stage, on the other hand, forest grassland was more suitable. Especially, during dry and high temperature season, temperature of soil surface and underground in the forest grassland were decreased by $6-7^{\circ}C$ and $3-4^{\circ}C$ each other, compared with those of the full-sunlight grassland. Also soil moisture content was continuously higher in the forest grassland. 2. At March 20 sowing the emergence time in the full-sunlight grassland was shortened by 8 days, compared with that of the forest grassland. In case of sowing on April 10, however, there was no difference between two grassland sites. 3. Grasses grown in the forest was more prostrate and leaves from them decayed more, compared with those of the full-sunlight grassland. 4. The percentage of grasses in the forest grassland was 80 to 85 %, on the other hand, that of the full-sunlight grassland was only 15 to 20 %. And the percentage of grasses tended to be high in the plot of early sowing time. 5. Dry root weight and root length of grasses grown in the forest were inferior to those of the full-sunlight grassland (P<0.05), but there was no significant difference between two sowing times. 6. Dry matter yield of grasses was significantly higher (P<0.05) in the forest grassland than in the full-sunlight grassland, and yield was influenced by sowing time. Higher yield (4,011 kg/ha) was produced in the plot of the forest grassland with early spring sowing. 7. From above results, it is suggested that grassland improvement by spring sowing in the forest is possible, and it is desirable to sow in early spring.
Carrot leaves have many nutrients as well as roots, which will increase the demand for carrot leaves in the future. This study was carried out by dividing into two stages: high temperature and low temperature periods, in order to investigate the possibility of cultivation of carrot leaves and the composition and EC of the nutrient solution for growth and quality of carrot leaves. Composition of nutrient solution($NO_3-N:16.0$, $NH_4-N:1.0$, P: 1.0, K: 11.0, Ca: 2.0, Mg: 1.0, $SO_4-S:1.0mM{\cdot}L^{-1}$) developed by analysis of plant. In the high temperature range (From June $29^{th}$ to Sep. $8^{th}$, 2016), the concentration of the developed nutrient solution (JNU) were 1.0, 2.0, 3.0, and $4.0dS{\cdot}m^{-1}$ and the concentration of nutrient solution of Japanese Horticultural Station(JHS) $2.0dS{\cdot}m^{-1}$ was used for comparison. In the low temperature range (From Dec. $31^{st}$, 2015 to Feb. $29^{th}$, 2016), the concentration of the developed nutrient solution 1.0, 2.0, and $3.0dS{\cdot}m^{-1}$ were used. Growth was investigated in root fresh and dry weights, shoot fresh and dry weights, leaf number, and leaf area of carrot. In the high temperature range, the leaf area and shoot fresh and dry weights were good at 1.0 and $2.0dS{\cdot}m^{-1}$. The sugar content of the root was the highest at the EC $2.0dS{\cdot}m^{-1}$, and the chlorophyll content was the highest at the EC $4.0dS{\cdot}m^{-1}$. In the low temperature range, The shoot fresh and dry weights were the highest at EC 1.0 and $2.0dS{\cdot}m^{-1}$. There was no significant difference in sugar content and chlorophyll content. As a result, from the viewpoint of growth and quality of carrot, it is good to cultivate EC 1.0 and $2.0dS{\cdot}m^{-1}$ in high temperature period and low temperature period, but EC $1.0dS{\cdot}m^{-1}$ is economical perspective such as fertilizer input.
In cold water irrigation, some growth and yield were decreased by heavy application of nitrogen but in-creased by heavy application of phosphate, potassium and silicate. Among growth characters, number of spikelets per panicle and grain filling ratio were affected significantly. Cold damage in number of spikelets, spikelet sterility and degeneration of spikelet and branch could be reduced by increasing application amount of phosphate, potassium and in particular silicate. Number of spikelets per branch was closely related with number of spikelets per secondary branches. Number of abortive grains and immature grains had negative correlations with yield and could be reduced by heavy application of phosphate, potassium and silicate. Heavy nitrogen application led to high total nitrogen content and restrained the uptake of phosphate, potassium and silicate. However, adverse results were showed by heavy application of phosphate, potassium and silicate. Inorganic element contents in branches were lower than those in leaf blades, but higher than those in chaff. Branches showed little differences in inorganic element contents between heading stage and maturing stage. Inorganic element contents in branches were considered to be influenced by those in leaf blades and to affect those in chaff. Some growth characters related to source and sink, such as degeneration of branches and spike-lets, sterility ratio, ripening ratio, and yield had closer relationship with nutrient contents in branches than those in leaf blades and chaff. The results demonstrated that the rachis branch not only was a transport pathway of nutrient but also would play an important role in accumulating substances in panicles.
It has been known that the silk degumming treated by hot alkali solution is easy to handle but is liable to yield poor-quality silk due to the degree of degumming loss, incomplete-degumming or over-degumming. Therefore, many studies have been carried out on the silk degumming by enzyme in order to improve the quality of silk. However, no attention has been paid to the physicochemical analysis of enzymatic degummed silk. In this paper, two different degumming methods, soap and enzymatic, are compared in aqueous solution state of silk fibroin. The results can be summarized as follows: There was no significant difference between two solutions on the bases of polarizing microscopy, TEM observation and SDS-PAGE. Spherulite of silk fibroin was not observed in polarizing microscopy, however the leaf-shape fibril structure was developed upon solidification. The size of spherulites of silk fibroin in TEM observation were 30~120nm with a wide range of size distribution. The intrinsic viscosity of enzymatic degummed fibroin solution was lower than that of soap degummed solution. This can be explained that the silk fibroin was more degraded by enzymatic degumming method compared with the soap degumming method. SDS-polyacrylamide gel electrophoresis showed that the fibroin molecule was composed of large component of molecule weight above 50 kd and small component of molecule weight about 20 kd. There was no difference in crystallinity between two degumming methods on the bases of results of DSC thermograms and IR spectra.
Journal of The Korean Society of Grassland and Forage Science
/
v.41
no.2
/
pp.134-140
/
2021
This study was carried out to study the effect of supplementary nitrogen fertilization application time according to regrowth date on growth date on growth characteristics, feed value, and productivity of Italian ryegrass (Lolium multiflorum Lam.; IRG) from 2019 to 2021 in Cheonan region. In order to determine the regrowth time, IRG was cut from experimental plots 3cm above soil level and the time when 1cm grew was judged as the regrowth time. The regrowth dates were February 15th and February 12th in 2020 and 2021 respectively. The experimental design was a randomized block design with three replications. The treatments were no fertilizer, immediately after the regrowth period, after 10 days of the regrowth period, and after 25 days of the regrowth period. Dry matter yield of IRG was significantly influenced by the supplementary N-fertilization application time. Dry matter yield was reduced for the delayed application time. The dry matter yield of immediately after the regrowth period was approximately 34.8 % higher than that no fertilizer application. In delaying the supplementary N-fertilization application time resulted in increased crude protein content. However, no significant statistical difference was neutral detergent fiber and acid detergent fiber(p>0.05). After wintering, productivity of IRG reduced for the delayed application time. Therefore, it is essential to application N-fertilizer immediately after the regrowth period for high productivity of IRG.
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