• 대한화장품학회지
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• 제32권4호
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• pp.249-255
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• 2006

본 연구는 백량금 추출물의 화장품 원료로서의 특성을 알아보기 위하여 항산화, 미백 및 항염 효과와 관련된 다양한 실험을 실시하였다. 70% 메탄을 추출물을 대상으로 실험한 결과 1,1-diphenyl-2-picryl-hydrazyl (DPPH) 라디칼 소거 효과는 0.01% 이상의 농도에서 90%의 효과를 나타내었고, 세포내 멜라닌 생성 억제 효과는 0.05% 이상의 농도에서 50% 이상의 효과를 나타내었다 이 후 70% 메탄을 추출물을 대상으로 MPLC를 사용하여 성분 분리 실험을 실시하여 5개의 분획들을 얻었고, 이들을 대상으로 실험한 결과 3번, 4번 그리고 5번 분획들에서 항산화(DPPH 라디칼 소거, Mn-SOD 생성 억제), 미백(멜라닌 생성 억제)그리고 항염($IL-1{\alpha}$, IL-6, COX-2, total NO 생성 억제)효과를 나타내었다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권1호
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• pp.79-85
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• 2015

One-day-old Cherry valley meat-strain ducks were used to investigate the effect of supplemental dried oregano powder (DOP) in feed on the productivity, antioxidant enzyme activity, and breast meat quality. One hundred sixty five ducks were assigned to 5 dietary treatments for 42 days. The dietary treatment groups were control group (CON; no antibiotic, no DOP), antibiotic group (ANT; CON+0.1% Patrol), 0.1% DOP (CON+0.1% DOP), 0.5% DOP (CON+0.5% DOP), and 1.0% DOP (CON+1.0% DOP). Upon feeding, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity of oregano extracts was higher than that of tocopherol, although it was less than that of ascorbic acid. As a result of in vivo study, DOP in the diet showed no effects on final body weight, feed intake, or feed conversion ratio. However, dietary 0.5% and 1% DOP supplementation caused a significant increase in the serum enzyme activity of superoxide dismutase (SOD) compared with CON and ANT, while glutathione peroxidase (GPx) in tissue was increased as compared to ANT (p<0.05). Cooking loss from ducks fed with DOP decreased compared with the control ducks. Thiobarbituric acid reactive substance (TBARS) values of duck breast meat at 5 d post slaughter was found to be significantly reduced in ducks whose diets were supplemented with 0.5% and 1% DOP (p<0.05). These results suggest that diets containing 0.5% and 1% DOP may beneficially affect antioxidant enzyme activity of GPx and SOD, improve meat cooking loss, and reduce TBARS values in breast meat at 5 d of storage in ducks.

• 생명과학회지
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• 제18권4호
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• pp.467-473
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• 2008

참나물의 생리활성 효과를 구명하기 위하여 in vitro에서 용매별 분획물의 항산화효능을 검토하였다. 참나물 에탄올추출물의 추출 수율은 12.01%이었으며, 이를 n-hexane, chloroform, ethylacetate, n-butanol, water로 계통 분획한 수율은 water 분획이 1.90%로 가장 높았으며, n-hexane, n-butanol, chloroform, ethylacetate 순이었다. In vitro에서 참나물 에탄올 추출물을 계통 분획하여 DPPH radical에 대한 자유기 소거능을 측정한 결과 n-hexane 분획이 $41\;{\mu}g/ml$로 가장 강한 항산화 활성을 나타내었다. 또한 Rancimat로 측정한 항산화지수도 n-hexane 분획이 1.98로 가장 높게 나타났으며, 이는 기존의 항산화제인 BHT가 1.97로 유사한 활성을 나타내었다. 아질산염 소거능에서도 모든 pH에서 n-hexane 분획이 가장 높게 나타났으며, pH 1.2에서 가장 우수하였다. 지질과산화물 생성 억제효과는 n-hexane 분획이 88.40%로 가장 높았으나, 양성대조군으로 사용한 BHT의 억제율보다도 낮았다. Linoleic acid에 대한 항산화효과를 지질과산화물가로 측정한 결과도 분획 중 n-hexane의 항산화력이 가장 우수하였고, ethylacetate, chloroform 순이었다. 이상의 결과 참나물은 식품으로의 이용가치뿐만 아니라 참나물 추출물의 in vitro 에서 항산화력의 우수함을 통해 천연 항산화제로서의 효과를 기대해 본다.

• Nutrition Research and Practice
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• 제9권6호
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• pp.569-578
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• 2015

BACKGROUND/OBJECTIVES: Fermentation can increase functional compounds in fermented soybean products, thereby improving antioxidant and/or anti-inflammatory activities. We investigated the changes in the contents of phenolics and isoflavones, antioxidant activity and anti-inflammatory activity of Doenjang during fermentation and aging. MATERIALS/METHODS: Doenjang was made by inoculating Aspergillus oryzae and Bacillus licheniformis in soybeans, fermenting and aging for 1, 3, 6, 8, and 12 months (D1, D3, D6, D8, and D12). Doenjang was extracted using ethanol, and sequentially fractioned by hexane, dichloromethane (DM), ethylacetate (EA), n-butanol, and water. The contents of total phenolics, flavonoids and isoflavones, 2,2-diphenyl-1 picryl hydrazyl (DPPH) radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured. Anti-inflammatory effects in terms of nitric oxide (NO), prostaglandin (PG) E2 and pro-inflammatory cytokine production and inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions were also measured using LPS-treated RAW 264.7 macrophages. RESULTS: Total phenolic and flavonoid contents showed a gradual increase during fermentation and 6 months of aging and were sustained thereafter. DPPH radical scavenging activity and FRAP were increased by fermentation. FRAP was further increased by aging, but DPPH radical scavenging activity was not. Total isoflavone and glycoside contents decreased during fermentation and the aging process, while aglycone content and its proportion increased up to 3 or 6 months of aging and then showed a slow decrease. DM and EA fractions of Doenjang showed much higher total phenolic and flavonoid contents, and DPPH radical scavenging activity than the others. At $100{\mu}g/mL$, DM and EA fractions of D12 showed strongly suppressed NO production to 55.6% and 52.5% of control, respectively, and PGE2 production to 25.0% and 28.3% of control with inhibition of iNOS or COX-2 protein expression in macrophages. CONCLUSIONS: Twelve month-aged Doenjang has potent antioxidant and anti-inflammatory activities with high levels of phenolics and isoflavone aglycones, and can be used as a beneficial food for human health.

• 한국축산식품학회지
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• 제32권5호
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• pp.612-617
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• 2012

The antioxidant, antimicrobial, and cytotoxic activities of ovotransferrin were investigated in vitro. The antioxidant capacity of ovotransferrin was evaluated using the 2,2-Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging method, antimicrobial effects using the agar well diffusion method, and cytotoxicity using the 3-(4,5-dimethylthizol-2-yl)-2,5-diphenylatetetrazolium bromide (MTT) assay. The DPPH radical-scavenging capacity of ovotransferrin at 1 mg/mL level reached approximately 60% after 48 h of reaction. The antimicrobial effects of ovotransferrin against common food-borne pathogens, Staphylococcus aureus KCCM 32395, Bacillus cereus KCCM 40935, Listeria monocytogenes ATCC 15313, Escherichia coli O157:H7 ATCC 43895, and Helicobacter pylori HpKCTC 26695 were dose dependant. Gram-positive bacteria was more sensitive to ovotransferrin than gram-negative bacteria. Ovotransferrin showed stronger antimicrobial effect against L. monocytogenes than other gram-positive bacteria tested. The cytotoxicity of ovotransferrin was evaluated in human cancer cell lines, various tissue origins, including the larynx (Hep-2), stomach (AGS), lung (SK-MES-1), liver (HepG2), breast (MCF-7), cervix (HeLa), and colon (HT-29). Ovotransferrin displayed relatively high cytotoxicity (${\leq}60%$ inhibition effects) at 40 mg/mL. At lower concentrations (${\leq}10mg/mL$), however, ovotransferrin cytotoxic effects were not significant in all cancer cell lines tested. These results indicated that ovotransferrin has potential to be used as an antioxidant or antimicrobial agent in foods or a pharmaceutical agent against cancers.

• 대한본초학회지
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• 제28권3호
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• pp.25-31
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• 2013

Objectives : To develop a natural antioxidant and anti-hemolytic agents, we investigated the effects of ethanol extracts of Carpesii Fructus and Farfarae Flos. Methods : Aerial parts of Carpesii Fructus and Farfarae Flos were extracted with 80% ethanol. Antioxidant activity of Carpesii Fructus or Farfarae Flos extract was evaluated by employing three different assays, i.e., 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-suphonic acid) diammonium (ABTS) scavenging and reducing power activities. Also, anti-hemolytic activity of Carpesii Fructus or Farfarae Flos extract was determined using [2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH)]-induced hemolysis, glutathione (GSH) depletion and malondialdehyde (MDA) formation in normal rat red blood cells (RBC) or plasma. Results : The extracts obtained from Carpesii Fructus and Farfarae Flos dose-dependently increased the scavenging activity on DPPH- or ABTS-induced radicals and the reducing power activities. Carpesii Fructus and Farfarae Flos were similar to the scavenging activity and the reducing power of butylated hydroxy anisole effect at high concentration ($1,000{\mu}g/mL$). RBC oxidative hemolysis and plasma MDA formation induced by AAPH were significantly suppressed by the extracts of Carpesii Fructus and Farfarae Flos in a dose-dependent manner. Also, Carpesii Fructus and Farfarae Flos extracts prevented the depletion of cystosolic antioxidant GSH in RBCs. Carpesii Fructus generally had better than the free radical scavenging activity, the reducing power and anti-hemolytic effects of Farfarae Flos. Conclusions : These results suggest that Carpesii Fructus and Farfarae Flos may have value as the potential antioxidant and anti-hemolytic medicinal plant.

• Advances in Traditional Medicine
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• 제7권1호
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• pp.85-93
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• 2007

Free radicals are known to play important role in pathophysiology of hepatic disorders and antioxidants are employed along with other chemotherapeutic agents in treatment of such diseases. In search of natural antioxidant, successive extracts of Hypericum (H.) hookerianum (Family: Hypericaceae) were evaluated by in vitro and in vivo methods. Extracts of aerial parts of H. hookerianum were subjected for 1,1-diphenyl 2-picryl hydrazyl radical scavenging activity (DPPH assay), nitric oxide radicals scavenging assay and thiobarbituric acid reactive substances (TBARS) assay. Methanolic extract was found to be more active than other extracts in DPPH and in vitro TBARS assay with $IC_{50}$ at 5.82 ${\pm}$ 1.33 ${\mu}g/ml$ and 49.78 ${\pm}$ 3.79 ${\mu}g/ml$ respectively. While petroleum ether extract showed more potentials in scavenging the nitric oxide radicals with $IC_{50}$ 220.97 ${\pm}$ 2.69 ${\mu}g/ml$. The administration of $CCl_{4}$ to the control animals caused decrease in the level of catalase and superoxide dismutase, together with significant increase in the level of TBARS in liver and kidney. Reversal of these changes towards normal group was observed by administration of H. hookerianum methanolic extract at 50 and 100 mg/kg body weight, while other extracts were found to be less active.

• 한국식품영양과학회지
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• 제30권1호
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• pp.133-137
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• 2001

21종 식용식물의 부위별 MeOH Ex. DPPH와 TBA법으로 항산화 효과를 조사한 결과 DPPH를 이용한 유리라디칼 소거 효과는 참취잎, 쑥갓잎 및 머위잎에서 특이적으로 높은 효과가 관찰되었으며 시료추출물의 buffer에 의해 유발된 과산화지질 억제효과는 참외껍질의 억제 효과 강도는 $IC_{50}$ 값이 $10\mu\textrm{g}/mL$로 매우 강하였다. $S_2O_z$로 유발시킨 지질과산화에 대한 억제효과는 0.25 mg 농도에서 L-ascorbic acid의 과산화 억제율과 비교하면 가지 잎, 머위 잎, 감자껍질의 항산화 억제 효과가 가장 높았으며 $FeSO_4$로 유발시킨 지질과산화에 대한 억제효과는 쑥갓잎의 $IC_{50}$가 0.08 mg/mL로 특이적으로 높았다.

• Journal of Acupuncture Research
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• 제20권4호
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• pp.42-52
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• 2003

Objective: For designing an optimal countermeasure against ageing and ageing related disease, it is necessary to understand the ageing mechanism and other reactive species. Free radicals are associated with the mechanisam of biological ageing and age-related diseases. This study was performed to identifiy the scavenging effect of Radix Cynanchi Wilfordii Herbal-Acupunture Solution(RCWHA) on Nitric Oxide(NO), 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) and Interleukin-4(IL -4). Methods: The activity to scavenge DPPH radicals and NO was observed in vitro, We evaluated the antioxidant activity of RCWHA by using DPPH or NO free radical method. Also the inhibitory effect on IL-4 in RCWHA was observed in vitro, Results: The followings are the summary of the results; (1) There is a significant scavenging effect of RCWHA on NO in 0.1mg/ml and 10mg/ml group after 24hrs. (2) There is no significant scavenging effect of RCWHA on DPPH. (3) There is a significant scavenging effect of RCWHA on IL-4 in $10{\mu}g/m{\ell}$ and $100{\mu}g/m{\ell}$ group. Conclusions: These findings indicate that RCWHA can be used as antioxidant or antiimflamatory drug. But further study is needed about the effect of RCWHA.

• 한국식품영양과학회지
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• 제37권1호
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• pp.76-82
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• 2008

찹쌀에 유자 과피를 각각 0, 10, 20, 30, 40% 첨가하여 제조한 진양주의 품질 및 생리활성 특성의 변화를 조사하였다. 유자 과피 첨가량의 증가에 따라 pH는 감소되었고 총산의 함량은 증가된 것으로 나타났다. 총당의 함량은 유자 과피의 첨가량의 증가에 따라 증가하였고 알코올 함량은 감소하였다. 색도 중 L값은 유자 과피의 첨가량이 증가에 따라 증가되었으나 a와 b값은 감소한 것으로 나타났다. DPPH 자유라디칼 소거 활성은 유자 과피의 첨가량의 증가에 따라 증가된 것으로 나타났다. 관능검사 결과 전체적 기호도에서 20% 유자 과피를 첨가하여 제조한 진양주가 제일 우수한 것으로 나타났다. 이화학적 특성, 항산화 특성, 관능적 특성으로 보아 20% 유자 과피를 첨가하여 진양주를 제조하는 것이 적합하다고 생각된다.