• 제목/요약/키워드: 01A13

검색결과 3,239건 처리시간 0.045초

한국인에서 중합효소반응을 이용한 short tandem repeat 유전좌위 F13A01 유전자형 및 대립유전자 빈도 (Genotype and Allele Frequency of the Short Tandem Repeat F13A01 Locus by Polymerase Chain Reaction in Korean)

  • Young-Su Lee;Chang-Lyuk Yoon
    • Journal of Oral Medicine and Pain
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    • 제21권2호
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    • pp.317-329
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    • 1996
  • Allelic frequency and genotype distribution of short tandem repeat(STR) F13A01 locus was analysed by polymerase chain reaction, polyacrylamide gel electrophoresis and silver staining from human genomic deoxyribonucleic acid(DNA) was extracted from 205 unrelated Korean to be applied to forensic identification and parentage testing as a database. The results were as follows : 1. 5 alleles and 11 genotypes of F13A01 locus were detected and heterozygosity value was 62.0% and the observed each alleles and allelic frequency was 3.2(0.363), 4(0.105), 5(0.063), 6(0.466), 16(0.002). 2. The allelic diversity value was 0.639 and the power of discrimination was 0.804.3. Compared with observed number of alleles and allele frequency in ethnic difference, result was appeared to be similar to that of Japanese and Asians, while was appeared to be much different to that of Blacks and Caucasians in the observed number of alleles and frequency of allele 3.2, 5, 7. From the above result of this investigation, the allelic frequency of STR F13A01 locus in the Korean was considerd to be useful for individual identification and parentage testing as a database.

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치석에서 Amelogenin Gene 및 Short Tandem Repeat(STR) 유전좌위 LPL, F13B, Triplex(F13A01, FESFPS, vWA)에 대한 분석 (Analysis of Amelogenin Gene and Short Tandem Repeat(STR) loci LPL, F13B, F13A01, FESFPS, vWA from the Dental Calculus)

  • 김상배;최종훈;윤창륙;김종열
    • Journal of Oral Medicine and Pain
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    • 제24권2호
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    • pp.219-234
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    • 1999
  • 치석에는 박리상피세포, 백혈구 등이 포함되어 있어 이들의 핵 내에 있는 DNA의 유전자형을 찾아내 개인식별을 할 수 있을 것으로 추정된다. 본 연구에서는 치석만으로 개인식별이 가능한지를 알아보고자 40명으로부터 채취한 치석을 증류수에 세척한 군과 세척하지 않은 군으로 나누어 DNA를 추출하고 중합효소연쇄반응법을 이용하여 증폭절편다형(AMP-FLPs)을 실시한 후 성별검사를 위한 X-Y homologous amelogenin gene과 유전자지문검사를 위한 STR유전좌위 LPL, F13B, Triplex(F13A01, FESFPS, vWA) 등 6개의 유전자를 검색하여 - X-Y homologous amelogenin gene과 LPL, F13B는 각각 증폭하였으며 F13A01, FESFPS, vWA 세 유전자는 동시에 증폭하였음 - 다음과 같은 결과를 얻었다. 1) X-Y homologous amelogenin gene 검색으로 세척군에서 27개의 검체 중 8개, 비세척군에서 13개 중 11개에서 성별검사가 가능하였다. 2) LPL유전자는 세척군, 비세척군에서 각각 27개 검체중 2개, 13개 검체 중 5개가 검색되었으며 3개의 대립유전자(10, 11, 12)와 4개의 유전자형 (10-10, 10-11, 10-12, 11-12)이 나타났다. 3) F13B유전자는 세척군, 비세척군에서 각각 27개 검체 중 1개, 13개 검체 중 4개가 검색되었으며 2개의 대립유전자(9, 10)와 2개의 유전자형(9-10, 10-10)을 관찰하였다. 4) F13A01유전자는 비세척군에서만 13개 검체 중 3개가 검색되었고 3개의 대립유전자(3.2, 4, 6)와 3개의 유전자형(3.2-3.2, 4-5, 4-6)을 관찰하였고, 세척군에서는 나타나지 않았다. 5) FESFPS유전자는 비세척군에서만 13개 검체 중 1개가 검색되었고 유전자 형은 11-12로 나타났다. 6) vWA유전자는 세척군, 비세척군에서 각각 1개씩 검색되었으며, 3개의 대립유전자형(14, 16, 17)와 2개의 유전자형(14-16, 14-17)을 관찰하였다. 이상의 결과를 종합해 볼 때, 치석은 X-Y homologous amelogenin gene증폭을 통한 성별검사와 단일 STR유전좌위 증폭을 통한 유전자지문형 검사에는 유용하나 복합 STR유전좌위의 검색에는 부적합한 것으로 나타났으며 법의학적시료로 응용이 가능한 것으로 사료된다.

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뇌정위적 방사선수술 시 콜리메이터 크기 변화에 따른 검출기 의존성 평가 (Evaluation of Detector Dependency on Collimator in SRS: Compared Detectors; CC01, CC13, SFD)

  • 배용기;방동완;박병문;강민영;김연래
    • 대한방사선치료학회지
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    • 제20권2호
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    • pp.109-113
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    • 2008
  • 목 적: 뇌정위적 방사선수술시 이용되는 콜리메이터의 크기 변화에 따른 검출기 의존성을 평가하고자 한다. 대상 및 방법: 본 실험을 위해 6 MV 광자선(CL21EX, Varian, Palo Alto), 이온전리함(CC01, CC13, Wellhofer, Germany), 정위적 다이오드검출기(SFD, Wellhofer, Germany)를 사용하였다. 심부선량백분율(PDD)의 검출기 의존성을 평가하기 위해 Brain Lab사의 콜리메이터(${\varphi}$5, 10, 20, 30 mm)와 $10{\times}10\;cm^2$에서 측정, 비교하였다. 선량측면도(dose profile)는 SAD 100 cm에서 콜리메이터(${\varphi}$10, 30 mm)와 $10{\times}10\;cm^2$를 측정하여 반치폭(FWHM; full width half maximum)과 반음영(20∼80%)을 비교하였다. 선량출력인자의 측정을 위해 선량최대깊이에서 콜리메이터(${\varphi}$5, 10, 20, 30 mm)의 출력을 측정하였으며, 정방형 조사면($10{\times}10\;cm^2$)의 출력 값을 기준으로 정규화 하였다. 결 과: 검출기에 따른 PDD의 영향을 평가하기 위해 PDD20,10 (PDD20/PDD10)을 비교하였다. 콜리메이터 ${\varphi}$5 mm에서는 SFD 51.3%, CC01 50%, CC13 58%로 측정되었고, 다른 콜리메이터에서는 최대 1%의 측정값 차이를 보였다. 선량측면도 평가에서 FWHM은 0.1∼0.4 mm의 차이를 보였다. 반음영은 ${\varphi}$10 mm에서 SFD 3.2 mm, CC01 7.1 mm, CC13 9.4 mm로 측정되었고, ${\varphi}$30 mm에서 SFD 4 mm, CC01 6.7 mm, CC13 12.4 mm로 측정되었다. $10{\times}10\;cm^2$에서는 SFD 6.6 mm, CC01 8.6 mm, CC13 12.2 mm로 측정되었다. 선량출력인자는 ${\varphi}$20 mm 이상에서 검출기별 최대 2%의 차이를 보였다. ${\varphi}$5 mm에서는 SFD 85%, CC01 61%, CC13 24%로 측정되었고, ${\varphi}$10 mm에서는 SFD 94%, CC01 85%, CC13 71%로 측정되었다. 실험결과 PDD는 CC13이 조사면에 충분히 포함되지 않은 ${\varphi}$5 mm에서 최대 16%의 차이를 보였고, 선량측면도의 FWHM은 검출기별 최대 0.4 mm의 차이를 보였다. 선량측면도의 반음영은 ${\varphi}$30 mm에서 최대 8.4 mm 차이를 보였고, 출력선량인자는 ${\varphi}$5 mm에서 최대 72%의 차이를 나타냈다. 결 론: 뇌정위적 방사선수술을 시행하는데 있어 정확한 선량을 측정할 수 있는 검출기를 선택하는 것은 무엇보다 중요하다. 따라서 본 실험에서는 측정을 통한 작은 원형조사면 선량계측에서 정위적 다이오드 검출기가 이온전리함에 비해 선량의 특성을 평가하는데 있어 유용함을 알 수 있었다.

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Characterization of CTL Clones Specific for Single Antigen, H60 Minor Histocompatibility Antigen

  • Jeon, Ji-Yeong;Jung, Kyung-Min;Chang, Jun;Choi, Eun-Young
    • IMMUNE NETWORK
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    • 제11권2호
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    • pp.100-106
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    • 2011
  • Background: Disparities of Minor H antigens can induce graft rejection after MHC-matched transplantation. H60 has been characterized as a dominant antigen expressed on hematopoietic cells and considered to be an ideal model antigen for study on graft-versus-leukemia effect. Methods: Splenocytes from C57BL/6 mice immunized with H60 congenic splenocytes were used for establishment of H60-specific CTL clones. Then the clones were characterized for proliferation capacity and cytotoxicity after stimulation with H60. Clone #14, #15, and #23 were tested for the TCR binding avidity to H60-peptide/$H-2K^b$ and analyzed for TCR sequences. Results: H60-specific CTL clones showed different levels of proliferation capacity and cytotoxic activity to H60-stimulation. Clones #14, #15, and #23 showed high proliferation activity, high cytotoxicity, and low activities on both aspects, respectively, and have TCRs with different binding avidities to H60-peptide/$H-2K^b$ with $t_{1/2}$ values of 4.87, 6.92, and 13.03 minutes, respectively. The TCR usages were $V{\alpha}12D-3-01+J{\alpha}11-01$ and $V{\beta}12-1-01+D{\beta}1-01+J2-7-01$ for clone #14, $V{\alpha}13D-1-02+J{\alpha}34-02$ and $V{\beta}13-1-02+D{\beta}2-01+J{\beta}2-7-01$ for clone #15, and $V{\alpha}16D+J{\alpha}45-01$ and $V{\beta}12-1-01+D{\beta}1-01+J{\beta}2-5-01$ for clone #23. Conclusion: The results will be useful for modeling GVL and generation TCR transgenic mouse.

경남지역 일부 직장인들의 근무형태와 건강상태, 식습관의 관련성 연구 (A Study on the Relationship between Working Patterns and Health Conditions and Eating Habits of Workers in the Gyeongnam Area)

  • 서은희
    • 한국식품영양학회지
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    • 제33권3호
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    • pp.266-278
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    • 2020
  • This study was conducted with 375 workers in Changwon to examine the effects of working patterns on dietary habits and health. A self-administered questionnaire was conducted July 22 October 30, 2019. According to the results of the dietary habits and health related factors analysis, non-shift administrative workers showed significantly higher rates of green tea intake (p<0.05), and shift production workers showed significantly higher smoking rates (p<0.001) and waist circumferences (p<0.01). According to the results of the Pearson's correlation coefficient analysis, work hours showed negative correlations with job satisfaction (r=0.22, p<0.01) and positive correlations with perceived stress level (r=0.14, p<0.01). Temporary workers showed negative correlations with feel job satisfaction(r=0.14, p<0.01), perceived stress level (r=0.12, p<0.05), and concern about health (r=0.13, p<0.05). Diabetes showed positive correlations with hypertension (r=0.20, p<0.01), low HDL cholesterolemia (r=0.22, p<0.01), abdominal obesity (r=0.13, p<0.05), and hypertriglyceridemia(r=0.22, p<0.01). Based on these results, this researcher proposes that continuous attention and support of industries and communities are necessary for nutritional education and counseling relative to improving workers' dietary lives as well as disease prevention and control.

청소년의 월경곤란증과 영향 요인 (Factors Affecting Dysmenorrhea among Adolescents)

  • 정향미;김이순
    • Child Health Nursing Research
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    • 제10권2호
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    • pp.196-204
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    • 2004
  • Purpose: This research was to determine the characteristics of dysmenorrhea among girls, identify the factors affecting dysmenorrhea. Method: The study subjects were 1653 high school student in Busan Metropolitan city. The data were collected by a self-administered questionnaire between November 1 and November 30, 2002. Descriptive statistics, t-test, one-way ANOVA with Scheffe' test, and stepwise multiple regression analysis in SPSS program were used for data analysis. Result: Students suffering from slight dysmenorrhea made up the majority 52.9%, whereas those with serious cases represented 29.8%. Regarding the measures taken to cope with dysmenorrhea, most of the respondents took analgesics 52.8%. A high points of dysmenorrhea was associated with irregular meal habits(p<0.01), unbalanced diet(p<0.05), stress(p<0.01), and health awareness(p<0.01), first menstruation before they reached 13 (p<0.01), heavy bleeding(p<0.01), family history(p<0.01). Factors affecting the dysmennorrhea of the respondents included the extent of bleeding, family history, health awareness, age during the first menstruation, and degree of stress, accounting for 13.1% of the total variance. Conclusion: To manage dysmenorrhea among girls, the results of the research indicate that students need to have proper awareness of health and manage stress through health education. Likewise, they need to be given positive follow-up management through a holistic nursing approach.

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말 제엽염의 침 치료 1례 (Acupuncture Treatment in a Case with Equine Laminitis)

  • 김덕환;유건주;최석화
    • 한국임상수의학회지
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    • 제23권1호
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    • pp.6-8
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    • 2006
  • 아일랜드 갈웨이주에서 사육중인 13살, 수컷, 아랍종말이 제엽염으로 진단되었다. TH01, SI01 및 ST45에 혈침 및 덱사메타손 수침(2회/주)을 각각 실시하였다. 또한 2회째 봉독(아피톡신 $200{\mu}g$을 동량의 리도카인에 혼합)을 GV01에 수침하였다. 3회 침치료 후 말은 정상 보행이 가능하였다.

Effects of Gal-13 on the Content of Immunoglobulin, Proliferation of Lymphocyte and Antibody Titers after Vaccination with Infectious Bursal Disease Virus Vaccine in Chickens

  • Yang, Yurong;Jiang, Yibao;She, Ruiping;Peng, Kaisong;Zhou, Xuemei;Yin, Qingqiang;Wang, Decheng;Liu, Tianlong;Bao, Huihui
    • Asian-Australasian Journal of Animal Sciences
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    • 제20권3호
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    • pp.405-411
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    • 2007
  • Gal-13 is an antimicrobial peptide isolated from chicken intestine. Ninety chickens were randomly divided into two groups (45 chickens for each group) to determine the effect of oral administration of Gal-13 on the acquired immune response. The chickens in the first group were fed a diet without Gal-13 as the control, and the chickens in the second group were fed the same diet, except that Gal-13 ($1{\mu}g/ml$) was suspended in drinking water just after hatching. Samples of blood, thymus, bursa of fabricius and spleen were taken at day 1, 4, 7, 10 and 17. The chickens in both groups received infectious bursal disease virus vaccine at day 20, and then sera samples were collected for analysis at 14, 21, 28 and 35 days after vaccination. The results showed: (1) Gal-13 could enhance the content of immunoglobulin (Ig)G at the age of 4 to10 days (p<0.05) and IgM at the age of 4 and 10 days (p<0.05) in the serum; (2) In vitro experiments showed that Gal-13 (0.625-1.250${\mu}g/ml$) enhanced the proliferation of peripheral blood lymphocytes of the chickens stimulated by lipopolysaccharide (LPS) and concanavlin A (ConA). Compared to the control, Gal-13 (1 ${\mu}g/ml$) enhanced the proliferation of bursa lymphocytes at 17 days of age (p<0.01) and thymus lymphocytes at 7 days of age (p<0.01), but restrained lymphocyte proliferation in chicken spleen and differed significantly at day 10 (p<0.01); (3) Gal-13 enhanced infectious bursal disease virus antibody in sera of chickens 21 days after infectious bursal disease virus vaccine administration (p<0.05). These results suggested that Gal-13 could modulate adaptive immune responses of chickens.

IP형 운반용기 차폐해석-잡고체폐기물을 중심으로 (Shielding Analysis for Industrial Package: Focusing on Dry Active Waste)

  • 이강욱;조천형;장현기;최병일;이흥영
    • 한국방사성폐기물학회:학술대회논문집
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    • 한국방사성폐기물학회 2005년도 춘계 학술대회
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    • pp.523-530
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    • 2005
  • 본 연구에서는 IP형 운반용기의 개념설계를 위하여 소내 임시저장중인 방사성폐기물중 $200\;{\ell}$ 잡고체 드럼을 대상으로 운반용기에 적재 가능한 드럼의 최대표면선량률을 제시하고자 하였다. 이를 위해 잡고체 폐기물을 가연성과 비가연성으로 구분하였으며, 각각 혼합핵종이 균일 분포되어있는 경우와 단일핵종(Co-60)이 균일 분포되어있는 경우를 나누어 계산하였다. 연구결과, 가연성 잡고체 드럼에 혼합핵종이 분포되어 있는 경우, IP-1, IP-2-a, IP-2-b형 운반용기에 적재 가능한 최대표면선량률은 각각 3.60E-01, 8.85E-01, 1.27E+01 mSv/hr 이었으며, Co-60이 분포되어 있는 가연성 잡고체 드럼의 최대표면선량률은 각각 3.59E-01, 8.83E-01, 1.25E+01 mSv/hr 이었다. 비가연성 잡고체 드럼에 혼합핵종이 분포되어 있는 경우, IP-1, IP-2-a, IP-2-b형 운반용기에 적재 가능한 최대표면선량률은 각각 7.14E-01, 1.83E+00, 2.69E+01 mSv/hr이었으며, Co-60이 분포되어 있는 비가연성 잡고체 드럼의 최대표면선량률은 각각 7.13E-01, 1.81E-01, 2.62E+01 mSv/hr 으로 계산되었다. 이를 통해 운반가능 방사성내용물의 최대수량을 실측이 용이한 표면선량률 만으로 제시할 수 있었으며, 향후 다른 종류의 폐기물에 대해서도 차폐해석을 수행할 예정이다.

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0.01 mm 급 우량계 개발에 관한 연구 (A Study on the Development of Raingauge with 0.01 mm Resolution)

  • 이부용
    • 한국환경과학회지
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    • 제13권7호
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    • pp.637-643
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    • 2004
  • A new method of automatic recording raingauge is developed to measure rainfall with 0.01mm resolution. This use two different signals to measure rainfall more accurately compare than other raingauges. One is weight of the tipping bucket with rainfall amount and the other is pulse from tipping bucket reverse. New method applied 1 mm tipping bucket mechanism and install loadcell under tipping bucket mechanism for measuring rainfall weight. Loadcell measure weight of rainfall until 1 mm with 0.01 mm resolution and more than 1 mm than bucket reverse and pulse signal generate, after that loadcell measure weight again. The validation of new instrument was examined in the room 65 mm/hour rainfall rate total 53 mm range. There is below than 1 % error of absolute rainfall amount and 0.01 mm resolution. The field test of instrument was carried out by comparing its measured values with values recorded by weight type and standard type on June 1 2003 at Terrestrial Environmental Research Center at Tsukuba University in Tsukuba of Japan, when it has recorded total amount of 40.58 mm rainfall by standard raingauge and new raingauge recorded 41.032 mm. Same rainfall intensity pattern observed in field observation with weight type raingauge. Rainfall intensity between weight type and Lee-A type raingauge reached 0.9947 correlation in 3 minute average.