• Biomolecules & Therapeutics
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• v.11 no.3
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• pp.169-173
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• 2003

Alkylthioureido-1,3-propandiols (KY353X series) were synthesized and evaluated as inhibitors for neutral sphingomyelinase (N-SMase). To examine whether KY353X series inhibit N-SMase, we purified the N-SMase from bovine brain. The N-SMase was partially purified by sequential chromatographies of DEAE-Cellulose anionic exchange and phenyl-5PW hydrophobic HPLC. These seqeuntial procedures for N-SMase resulted in a 67-fold purification and excluded other isoforms of SMase. Based on in vitro assay, KY353X series inhibited N-SMase activity in time, concentration-dependent manners and completely inactivated N-SMase at 50 $\mu$M. In particular, KY3535 and KY3536 inhibited more effectively than the others. To further determine the .inhibitory pattern, a Dixon plot was constructed, to showing that the inhibition by KY3535 and KY3536 were competitive. The inhibition constant (Ki) of KY3535 and KY3536 was 1.7 $\mu$M and 2.5$\mu$M in 100 mM Tris-HCl buffer, pH 7.0, respectively.

• Journal of the Korean Ceramic Society
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• v.52 no.6
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• pp.514-520
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• 2015

$KY_{1-x}(WO_4)_2:Ho^{3+}/Yb^{3+}$ yellow phosphors with doping concentrations of $Ho^{3+}$ and $Yb^{3+}$ ($x=Ho^{3+}+Yb^{3+}$, $Ho^{3+}=0.05$, 0.1, 0.2 and $Yb^{3+}=0.2$, 0.45) were successfully prepared using the microwave-modified sol-gel method; their upconversion (UC) photoluminescence properties were investigated in detail. Well-crystallized particles, formed after heat-treatment at $900^{\circ}C$ for 16 h, showed a fine and homogeneous morphology with particle sizes of $2-5{\mu}m$. Under excitation at 980 nm, the UC $KY_{0.7}(WO_4)_2:Ho_{0.1}Yb_{0.2}$ and $KY_{0.5}(WO_4)_2Ho_{0.05}Yb_{0.45}$ particles exhibited excellent yellow emissions based on a strong 545-nm emission band in the green region and a very strong 655-nm emission band in the red region. Pump power dependence and Commission Internationale de L'Eclairage chromaticity of the UC emission intensity were evaluated. The spectroscopic properties were examined comparatively using Raman spectroscopy.

• Korean Journal of Food Science and Technology
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• v.31 no.2
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• pp.495-501
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• 1999

Lactobacillus acidophilus KY 2104 was isolated from 41 healthy korean ($2{\sim}35\;age$, 26 man, 15 woman) feces to use as a probiotic lactic acid bacteria for functional foods, fermented milk and pharmaceutical products. L. acidophilus KY 2104 survived in the presence of acid and bile, and inhibited pathogens. It survived almost 100% in pH 3.0 phosphate buffer for 2 hr, and more than $10^4\;cfu/mL$ in pH 2.0 phosphate buffer when inoculated by $10^7\;cfu/mL$. It was also found to grow in MRS medium containing 0.5% oxgall. L. acidophilus KY 2104 exerted inhibitory effect against enteric pathogens such as Escherichia coli, Shigella dysenteriae, Staphylococcus aureus and Salmonella typhimurium, when grown with each in associative cultures for 16 hr. As a result of acute toxicity of L. acidophilus KY 2104 in rats, there was no observation on motality, clinical signs, body weights and gross findings in the oral administration.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.297-302
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• 2004

A chitinase-producing bacterium was isolated from seashore mud around Beobseongpo in Chunmam province through the use of a selective enrichment culture. The best chitinase producing strain was isolated and identified as Serratia marcescens KY from its characteristics. For effective production of chitinase, optimum pH, temperature, and agitation speed were investigated in flask cultures. The optimum pH using Serratia marcescens KY was between pH 6 and 7 and the chitinase produced was 37.9 unit/mL. On the other hand, the optimal pH of the Serratia marcescens ATCC 27117 was 7.5, and the produced amount of chitinase was 35.2 unit/mL. The optimal temperature for chitinase production for Serratia marcescens KY and Serratia marcescens ATCC 27117 was $30^{\circ}$. The cell growth pattern at different temperature was almost identical to the chitinase production. To investigate the optimal shaking speed under optimal culture, speeds were varied in the range of 0∼300 rpm. The maximum production of chitinase was carried at 200 rpm although the cell growth was the highest at 150 rpm. It indicates that oxygen adjustment is required for the maximum chitinase production. Using optimal conditions, batch cultures for comparing Serratia marcescens KY and Serratia marcescens ATCC 27117 were carried out in a 5 L fermentor. The oxygen consumption was increased with the increase of culture. Especially, at 120 h of culture Serratia marcescens KY and Serratia marcescens ATCC 27117 produced 38.3 unit/mL, and 33.5 unit/mL, respectively.

• Economic and Environmental Geology
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• v.52 no.5
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• pp.409-425
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• 2019

Marine terraces, a step-like landform, are important geologic markers that provide tectonic information during the Quaternary Period. Marine terraces are well developed along all coastlines(East, West, and South) of the Korean Peninsula, those along the East coastline are the most distinctive. The marine terraces of the East coastline are classified into 4-6 flights that are several meters or several tens of meters above the present sea level. It is believed that these terraces, except for the lowest one, were formed in the middle Pleistocene. In the base of the OSL age dating results and Blake excursion events of magnetostratigraphy, the $2^{nd}$ and $3^{rd}$ terraces are correlated to the last interglacial stage. Considering the marine terraces linked to a sea-level curve of the Pleistocene, it is thought that regional tectonic movements have uplifted the East coastal area since the middle Pleistocene. Besides, former shorelines of each terrace have varied elevations from Gangreung to Busan bay, which can be divided into four regions, namely, Gangreung-Yonghanri(I), Homikot-Najung(II), Najung-Bangeojin(III), and Waesung-Busan Bay(IV). The former shorelines of each terrace at both Gangreung-Yonghanri(I) and Najung-Bangeojin(III) are higher than those in the other two regions, due to block movements by regional faults such as the Ocheon Fault or its subsidiaries, the Gampo Lineament and Ulsan Fault. Uplift rate of the East coast ranges from 0.2 m/ky to 0.3 m/ky, but each region shows different uplift rate.

• Economic and Environmental Geology
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• v.36 no.3
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• pp.233-242
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• 2003

The southeasternmost coastal area of the Korean peninsula has been regarded as a seismologically stable area as neither Quaternary faults nor earthquake activity has been reported. To clarify whether the active tectonic movement has occurred or not, a digital marine terrace mapping and fracture mapping have been done in the coastal area. Bed rocks are composed of the Cretaceous volcanic and sedimentary rocks and the Paleogene granite. Wave-cut platform in the area is smaller and narrower relative to that of the northern coastal area. Most of the platforms in the area have little Quaternary sediment. The platforms except the Holocene terrace (1 st terrace) can be divided into three steps. The lowest platform (2nd terrace) has an altitude of 8-11 m. The broad middle one (3rd terrace) is 17 to 22 m high. The highest terrace (4th terrace) is a narrow and sporadic bench with an altitude of about 44 m high. The lowest terrace is correlated to the 2nd terrace of the northern area, which corresponds to the oxygen isotopic stage 5a. The uplift rate calculated from a graphic method is 0.19 m/ky. This low uplift is typical of an intra-plate, suggesting that the area is tectonically stable. The elevation of the platforms tends slightly lower from the north to the south in the survey area. The decreasing altitude of the platforms towards the south is interpreted to result from a local block tilting during the Latest Pleistocene. This also indicates that the eastern coast of the Korean peninsula has been suffering a subsidence to the south.

• Journal of Microbiology and Biotechnology
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• v.3 no.1
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• pp.31-38
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• 1993

A bacterial strain KY-l isolated from sewage was able to produce an extracellular agarase(agarose 4-glycanohydrolase. EC 3.2.1.81). The strain KY-1 was identified as Cytophaga fermentans subsp. agarovorans based on its morphological and physiological characteristics. The agarase was purified by ammonium sulfate precipitation followed by DEAE-Sephadex A-50. Bio-Gel P-100. and CM-Cellulose column chromatography. The molecular weight of the purified enzyme was 24 kDa by SDS-polyacrylamide gel electrophoresis. The optimum temperature and pH for the enzyme activity were 30^{circ}C and 7.5, respectively. The enzyme activity was significantly inhibited in the presence of 0.1 mM $HgCl_2$. whereas it was elevated 3 times by $MnSO_4$ at 1 mM concentration. The Km value and Vmax were 16.67 mg/ml and 3.77 unit/ml.min. The agarase gene was cloned into Escherichia coli MC1061 using the plasmid vector pBR322. A 1.4 Kb DNA fragment of PstI-digested chromosomal DNA of C. fermentans KY-l was inserted into the PstI site of pBR322. expressed in the E. coli. and up to 60% of the total enzyme was extracellularly secreted. Enzymatic properties of the extracellular agarases produced by both the transformant and the donor were very similar in terms of optimal pH and temperature.

• Proceedings of the KSEEG Conference
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• 2003.04a
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• pp.131-134
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• 2003

계기지진기록에 의하면, 한반도에서는 중국이나 일본만큼 지진발생빈도가 높거나 강진은 없으나, 북한에서는 추가령단층대 이서지역, 남한에서는 아산만～영일만 일원을 따라 대상으로 분포한다. 역사지진기록에 의하면, 경주시 주변에서 8개의 큰 지진이 일어났으며, 이중 779년에 일어난 경주지진에서는 백명 이상의 사상자가 발생한 기록이 있다고 하였다(경재복, 1997). (중략)

• Journal of Korean Society of Environmental Engineers
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• v.35 no.9
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• pp.654-659
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• 2013

This study was conducted to identify an optimal ratio of carbon to nitrogen (C/N ratio) for denitrification of nitrate using molasses as an external carbon source. A series of batch and column tests was conducted using an indigenous bacterium Pseudomonas sp. KY1 isolated from a nitrate-contaminated soil. For the initial nitrate-nitrogen concentration of 100 mg-N/L, batch test results indicated that C/N ratio of 3/1 was the optimal ratio with a relatively high pseudo-first-order reaction constant of $0.0263hr^{-1}$. At C/N ratio of 3/1, more than 80% of nitrate-nitrogen concentration of 100 mg-N/L was removed in 100 hrs. Results of column tests with a flow velocity of 0.3 mL/min also indicated that the C/N ratio of 3/1 was optimal for denitrification with minimizing remaining molasses concentrations. After 172 hrs of column operation (35 pore volumes) with an influent nitrate-nitrogen concentration of 100 mg-N/L, the effluent met the drinking water standard (i.e., 10 mg $NO_3$-N/L).

• Applied Chemistry for Engineering
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• v.8 no.2
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• pp.308-314
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• 1997

The effects of catalyst and blocking reagents(BR) on the blocking reactions of TDI and MDI in the preparation of polyurethane varnishes have been studied. It was found that certain types of catalysts, such as 1,4-diazabicyclo[2.2.2]octane(DABCO) and tetramethyl guanidine(TMG), were necessary to complete the NCO blocking reactions. Blocking rate of TMG was shown much faster than that of DABCO. Reactivity increased with the increase of TMG concentration, at least to the concentration of 1%. In case of DABCO, the 0.25% concentration of catalyst showed the highest reactivity. In order to study the steric effects of BR on the blocking reactions, phenol, p-cresol, m-cresol, o-cresol, 2,4-xylenol were used as blocking reagents. As expected, BR which has less steric hindrance showed higher reactivity. The increase in BR concentration and reaction temperature increased the productivity of polyurethanes in this reaction system.