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Oligosaccharide Production by Leuconostoc lactis CCK940 Which Has Glucansucrase Activity (Leuconostoc lactis CCK940의 Glucansucrase 활성에 의한 올리고당 생산 최적화)

  • Lee, Sulhee;Park, Young-Seo
    • Food Engineering Progress
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    • v.21 no.4
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    • pp.383-390
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    • 2017
  • Glucansucrase is an enzyme classified as a glycoside hydrolase (GH) 70 family, which catalyzes the synthesis of glucooligosaccharides with a low molecular weight using sucrose as a donor of D-glucopyranose and maltose as a carbohydrate acceptor. In this study, glucansucrase-producing lactic acid bacteria strain was isolated from the fermented foods collected in traditional markets, and the optimum conditions for the oligosaccharide production were investigated. The strain CCK940 isolated from Chinese cabbage kimchi was selected as an oligosaccharide-producing strain due to its high glucansucrase activity, with 918.2 mU/mL, and identified as Leuconostoc lactis. The optimum conditions for the production of oligosaccharides using Leu. lactis CCK940 were to adjust the initial pH to 6.0, add 5% (w/v) sucrose and 10% (w/v) maltose as a donor and acceptor molecules, respectively, and feed 5% (w/v) sucrose at 4 and 8 h of cultivation. When Leu. lactis CCK940 was cultured for 12 h at optimum conditions, at least four oligosaccharides with a polymerization degree of 2-4 were produced.

COVID-19 Lung CT Image Recognition (COVID-19 폐 CT 이미지 인식)

  • Su, Jingjie;Kim, Kang-Chul
    • The Journal of the Korea institute of electronic communication sciences
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    • v.17 no.3
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    • pp.529-536
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    • 2022
  • In the past two years, Severe Acute Respiratory Syndrome Coronavirus-2(SARS-CoV-2) has been hitting more and more to people. This paper proposes a novel U-Net Convolutional Neural Network to classify and segment COVID-19 lung CT images, which contains Sub Coding Block (SCB), Atrous Spatial Pyramid Pooling(ASPP) and Attention Gate(AG). Three different models such as FCN, U-Net and U-Net-SCB are designed to compare the proposed model and the best optimizer and atrous rate are chosen for the proposed model. The simulation results show that the proposed U-Net-MMFE has the best Dice segmentation coefficient of 94.79% for the COVID-19 CT scan digital image dataset compared with other segmentation models when atrous rate is 12 and the optimizer is Adam.

STRONG PRESERVERS OF SYMMETRIC ARCTIC RANK OF NONNEGATIVE REAL MATRICES

  • Beasley, LeRoy B.;Encinas, Luis Hernandez;Song, Seok-Zun
    • Journal of the Korean Mathematical Society
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    • v.56 no.6
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    • pp.1503-1514
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    • 2019
  • A rank 1 matrix has a factorization as $uv^t$ for vectors u and v of some orders. The arctic rank of a rank 1 matrix is the half number of nonzero entries in u and v. A matrix of rank k can be expressed as the sum of k rank 1 matrices, a rank 1 decomposition. The arctic rank of a matrix A of rank k is the minimum of the sums of arctic ranks of the rank 1 matrices over all rank 1 decomposition of A. In this paper we obtain characterizations of the linear operators that strongly preserve the symmetric arctic ranks of symmetric matrices over nonnegative reals.

Purification and Characterization of Two Thermostable Xylanases from Paenibacillus sp. DG-22

  • Lee, Yong-Eok;Lim, Pyung-Ok
    • Journal of Microbiology and Biotechnology
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    • v.14 no.5
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    • pp.1014-1021
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    • 2004
  • Two thermostable xylanases, designated XynA and XynB, were purified to homogeneity from the culture supernatant of Paenibacillus sp. DG-22 by ion-exchange and gel-filtration chromatography. The molecular masses of xylanases A and B were 20 and 30 kDa, respectively, as determined by SDS-PAGE, and their isoelectric points were 9.1 and 8.9, respectively. Both enzymes had similar pH and temperature optima (pH 5.0-6.5 and $70^{\circ}C$), but their stability at various temperatures differed. Xylanase B was comparatively more stable than xylanase A at higher temperatures. Xylanases A and B differed in their $K_m$ and $V_{max}$ values. XynA had a $K_m$ of 2.0 mg/ml and a $V_{max}$ of 2,553 U/mg, whereas XynB had a K_m$ of 1.2 mg/ml and a $V_{max}$, of 754 U/mg. Both enzymes were endo-acting, as revealed by their hydrolysis product profiles on birchwood xylan, but showed different modes of action. Xylotriose was the major product of XynA activity, whereas XynB produced mainly xylobiose. These enzymes utilized small oligosaccharides such as xylotriose and xylotetraose as substrates, but did not hydrolyzed xylobiose. The amino terminal sequences of XynA and XynB were determined. Xylanase A showed high similarity with low molecular mass xylanases of family 11.

Theoretical Study on the Nonadiabatic Transitions in the Photodissociation of Cl2, Br2, and I2

  • Asano, Yukako;Yabushita, Satoshi
    • Bulletin of the Korean Chemical Society
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    • v.24 no.6
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    • pp.703-711
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    • 2003
  • We have theoretically studied the nonadiabatic transitions among the five lower states with the Ω=$1_u$ symmetry ($1_u^{(1)} to 1_u^{(5)}$) in the photodissociation of Cl₂, Br₂, and I₂by using the spin-orbit configuration interaction (SOCI) method and the semiclassical time-dependent coupled Schrodinger equations. From the configuration analyses of the SOCI wavefunctions, we found that the nonadiabatic transition between $1_u^{(2)}$ and $1_u^{(1)}$ is a noncrossing type, while that between $1_u^{(3)}$ and $1_u^{(4)}$ is a crossing type for all the molecules. The behavior of the radial derivative coupling element between $1_u^{(1)}$ and $1_u^{(2)}$ and that between $1_u^{(3)}$ and $1_u^{(4)}$ is analyzed in detail. In Cl₂, nonadiabatic transitions can take place even between the states correlating to different dissociation limits, while in Br₂ and I₂, with the usual photon energies e.g. less than 20 eV, nonadiabatic transitions occur only between the states correlating to the same dissociation limits, reflecting the different magnitudes of the spin-orbit interactions.

The Quantitative Analysis of the Superposed Ultrasonic Signal by PD Sources in a Transformer (부분방전원에 의해 중첩된 초음파신호의 정량적분석)

  • 송현석;구교선;이동준;곽희로
    • Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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    • 2001.11a
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    • pp.163-166
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    • 2001
  • In this paper, the superposition of ultrasonic signals which were generated by multi-source in a transformer was presented. For analysis of ultrasonic superposition, V$_{peak-peak}$ and pulse number of ultra-sonic signal were used. As a result, the wave form of ultrasonic superposition was classified into three types ; First type was the wave form whose V$_{peak-peak}$ was increased by the superposition of each signal's V$_{peak-peak}$. Second type was wave form whose V$_{peak-peak}$ was decreased by the superposition of each signal's V$_{peak-peak}$. And the other type was wave form whose peals were two or mere. And V$_{peak-peak}$ of superposed ultrasonic wave wads mostlv increased or decreased and pulse number of supe게osed u1trasonic wave was mostly increased.y increased.

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LINEAR PRESERVERS OF SYMMETRIC ARCTIC RANK OVER THE BINARY BOOLEAN SEMIRING

  • Beasley, LeRoy B.;Song, Seok-Zun
    • Journal of the Korean Mathematical Society
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    • v.54 no.4
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    • pp.1317-1329
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    • 2017
  • A Boolean rank one matrix can be factored as $\text{uv}^t$ for vectors u and v of appropriate orders. The perimeter of this Boolean rank one matrix is the number of nonzero entries in u plus the number of nonzero entries in v. A Boolean matrix of Boolean rank k is the sum of k Boolean rank one matrices, a rank one decomposition. The perimeter of a Boolean matrix A of Boolean rank k is the minimum over all Boolean rank one decompositions of A of the sums of perimeters of the Boolean rank one matrices. The arctic rank of a Boolean matrix is one half the perimeter. In this article we characterize the linear operators that preserve the symmetric arctic rank of symmetric Boolean matrices.

THE PRIMITIVE BASES OF THE SIGNED CYCLIC GRAPHS

  • Kim, Byeong Moon;Song, Byung Chul
    • Korean Journal of Mathematics
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    • v.21 no.1
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    • pp.55-62
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    • 2013
  • The base $l(S)$ of a signed digraph S is the maximum number $k$ such that for any vertices $u$, $v$ of S, there is a pair of walks of length $k$ from $u$ to $v$ with different signs. A graph can be regarded as a digraph if we consider its edges as two-sided arcs. A signed cyclic graph $\tilde{C_n}$ is a signed digraph obtained from the cycle $C_n$ by giving signs to all arcs. In this paper, we compute the base of a signed cyclic graph $\tilde{C_n}$ when $\tilde{C_n}$ is neither symmetric nor antisymmetric. Combining with previous results, the base of all signed cyclic graphs are obtained.

Shielding 140 keV Gamma Ray Evaluation of Dose by Depth According to Thickness of Lead Shield (140 keV 감마선 차폐 시 납 차폐체 두께에 따른 깊이별 선량 평가)

  • Kim, Ji-Young;Lee, Wang-Hui;Ahn, Sung-Min
    • Journal of radiological science and technology
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    • v.41 no.2
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    • pp.129-134
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    • 2018
  • The present study made a phantom for gamma ray of 140 keV radiated from $^{99m}Tc$, examined shielding effect of lead by thickness of the shielding material, and measured surface dose and depth dose by body depth. The OSL Nano Dot dosimeter was inserted at 0, 3, 15, 40, 90, and 180 mm depths of the phantom, and when there was no shield, 0.2 mm lead shield, 0.5 mm lead shield, The depth dose was measured. Experimental results show that the total cumulative dose of dosimeters with depth is highest at 366.24 uSv without shield and lowest at 94.12 uSv with 0.5 mm lead shield. The shielding effect of 0.2 mm lead shielding was about 30.18% and the shielding effect of 0.5 mm lead shielding was 74.30%, when the total sum of the accumulated doses of radiation dosimeter was 100%. The phantom depth and depth dose measurements showed the highest values at 0 mm depth for all three experiments and the dose decreases as the depth increases. This study proved that the thicker a shielding material, the highest its shielding effect is against gamma ray of 140 keV. However, it was known that shielding material can't completely shield a body from gamma ray; it reached deep part of a human body. Aside from the International Commission on Radiation Units and Measurements (ICRU) recommending depth dose by 10 mm in thickness, a plan is necessary for employees working in department of nuclear medicine where they deal with gamma ray, which is highly penetrable, to measure depth dose by body depth, which can help them manage exposed dose properly.

THE BASES OF PRIMITIVE NON-POWERFUL COMPLETE SIGNED GRAPHS

  • Song, Byung Chul;Kim, Byeong Moon
    • Korean Journal of Mathematics
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    • v.22 no.3
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    • pp.491-500
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    • 2014
  • The base of a signed digraph S is the minimum number k such that for any vertices u, v of S, there is a pair of walks of length k from u to v with different signs. Let K be a signed complete graph of order n, which is a signed digraph obtained by assigning +1 or -1 to each arc of the n-th order complete graph $K_n$ considered as a digraph. In this paper we show that for $n{\geq}3$ the base of a primitive non-powerful signed complete graph K of order n is 2, 3 or 4.