• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.4
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• pp.389-396
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• 2015

This study examined how to improve the Korean Navy diving system, particularly focused on using the mobile saturation diving system. This study determined the range of navy operations, and suggested requirements of mobile saturation diving system from the analysis of previous study and current Navy Diving system. Also, this study examined the use of military strategy in terms of its technical and applicable possibility for the enhancement of current system. In addition, the study analyzed both advantages/disadvantages of importing mobile saturation diving system products and domestic development. To review measures in installing and operating such system, this study included analysis on the effectiveness of individual platform. As the result, the study suggested requirements of mobile saturation diving system to be able to be operated by more than 6 divers for more than 17 days at more than 200m depth. And the study confirmed that there are beneficial to use mobile saturation diving system and current Navy saturation diving system together. However, it is low economical efficiency to change current Navy gas diving system to Saturation diving system. To review measures in installing and operating such system, this study suggested that second ATS-II should be built to be able to install mobile saturation diving system. Also, this study generated an utilization method of the system to use it for training when peacetime, and than it should be installed on other platform to use as additional salvage strength during wartime.

• Fire Science and Engineering
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• v.34 no.2
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• pp.72-85
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• 2020

This article is a study on the activity of rescue workers for designing simulation training scenarios assuming chemical accidents. On the basis of the complexity of the indoor scene in the case of chemical accidents and terrorism, we designed a 12-step simulation training scenario for two teams to analyze the improvement in firefighters' capabilities. On the basis of activity measurement in the simulation scenario, step 2 of training had the most drops in the maximum heart rate, as follow: N1, from 163 bpm to 153 bpm; N2, from 186 bpm to 151 bpm; N3, from 168 bpm to 162 bpm; and N4, from 166 bpm to 152 bpm. In terms of intensity level in the allowable activity time, it was found that in step 2 both N1 and N2 reduced from Level 5 to Level 3, N3 remained at Level 4, N4 reduced from Level 4 to Level 3, and the maximum allowable activity time increased.

• Fire Science and Engineering
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• v.33 no.6
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• pp.165-172
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• 2019

This study presents the design and manufacture of a self-contained breathing apparatus SCBA wireless communication module with a multipoint simultaneous full-duplex communication system to enable communication between team members wearing the SCBA system. It is necessary for fire-fighters to wear the SCBA system during extinguishing and rescue work at the fire site. Evaluation of the team communication module confirmed the feasibility of communication over more than 500 m in the test condition based on the line of sight. By implementing the Ad-hoc function, it was confirmed that the communication distance could be extended to 128 m by automatic routing up to 3 hoc. The vertical distance inside the building for successful communication was up to the 5th floor in the open staircase and up to the 3rd floor in the partitioned staircase. Furthermore, the performance testing of the communication module assuming a fire situation, confirmed that five team members correctly recognized the standard abbreviation of fire and wireless communication without a separate PTT key operation. In addition, the flame resistance was verified by exposing the module to a flame at 950 ± 50 ℃ for 5 s and then immediately extinguishing the flame.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.5
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• pp.635-647
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• 2014

Unfertilized oocytes age inevitably after ovulation, which limits their fertilizable life span and embryonic development. Rapamycin affects mammalian target of rapamycin (mTOR) expression and cytoskeleton reorganization during oocyte meiotic maturation. The goal of this study was to examine the effects of rapamycin treatment on aged porcine oocytes and their in vitro development. Rapamycin treatment of aged oocytes for 24 h (68 h in vitro maturation [IVM]; $44h+10{\mu}M$ rapamycin/24 h, $47.52{\pm}5.68$) or control oocytes (44 h IVM; $42.14{\pm}4.40$) significantly increased the development rate and total cell number compared with untreated aged oocytes (68 h IVM, $22.04{\pm}5.68$) (p<0.05). Rapamycin treatment of aged IVM oocytes for 24 h also rescued aberrant spindle organization and chromosomal misalignment, blocked the decrease in the level of phosphorylated-p44/42 mitogen-activated protein kinase (MAPK), and increased the mRNA expression of cytoplasmic maturation factor genes (MOS, BMP15, GDF9, and CCNB1) compared with untreated, 24 h-aged IVM oocytes (p<0.05). Furthermore, rapamycin treatment of aged oocytes decreased reactive oxygen species (ROS) activity and DNA fragmentation (p<0.05), and downregulated the mRNA expression of mTOR compared with control or untreated aged oocytes. By contrast, rapamycin treatment of aged oocytes increased mitochondrial localization (p<0.05) and upregulated the mRNA expression of autophagy (BECN1, ATG7, MAP1LC3B, ATG12, GABARAP, and GABARAPL1), anti-apoptosis (BCL2L1 and BIRC5; p<0.05), and development (NANOG and SOX2; p<0.05) genes, but it did not affect the mRNA expression of pro-apoptosis genes (FAS and CASP3) compared with the control. This study demonstrates that rapamycin treatment can rescue the poor developmental capacity of aged porcine oocytes.

• Korean Journal of Environment and Ecology
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• v.28 no.5
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• pp.523-530
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• 2014

One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.6
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• pp.106-112
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• 2016

In this study, circular concrete column specimens confined by carbon sheet tube with different winding angles and different number of carbon sheet plies(3T, 5T and 7T) were tested to propose design equations and a strength reduction factor. Specimens were designed by 300 mm diameter and 600 mm height with $90^{\circ}{\pm}0^{\circ}$, $90^{\circ}{\pm}30^{\circ}$, $90^{\circ}{\pm}45^{\circ}$, $90^{\circ}{\pm}60^{\circ}$, $90^{\circ}{\pm}75^{\circ}$ and $90^{\circ}{\pm}90^{\circ}$ carbon fiber angles. A 10,000 kN UTM was used for compressive strength test of specimens by displacement control method with 0.01 mm/sec velocity. Estimation equations of compressive strength and ultimate strain of circular concrete column specimens confined by carbon sheet tube using a regression analysis and a strength reduction factor to apply ultimate strength design method of concrete were proposed. The strength reduction factor(${\phi}$) of circular concrete columns confined by carbon sheet tube was estimated as 0.64 by the Monte Carlo Analysis Method. Manufacture and construction process have to be perfectly managed by construction managers because the structural capacities of carbon tubes were depends on construction abilities of manufacturing operators.

• Fire Science and Engineering
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• v.31 no.3
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• pp.106-118
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• 2017

The purpose of this study was to realize volunteer fire departments' historical identity through a review of the transition process from the quickening period of volunteer fire departments to the enactment of the volunteer fire department act in 2014, establish it newly from the viewpoint of changing the basic theoretical concepts, and seek a development plan. For this, the technical approach method was used based on the research method of literature searches. The result of the study established the definition as 'volunteer fire departments are legal auxiliary organizations composed by local residents who volunteer and understand one another with their independent and voluntary will to prepare for all kinds of disasters as well as fire suppression, rescue, and first aid.' and the installation ideology as 'leading service organizations of safety as a form of community involvement', 'resident-friendly organizational activities based on autonomous operational operation' and 'the activities of supplementing their demands according to the realization of social welfare and regional conditions'. Regarding the characteristics, the basic theoretical concept was newly established by 'voluntary participation', 'social expandability', 'the realization and complementation of social welfare', and 'professionalism and possibilities'. In addition it is expected that the results of this study can be utilized as the basic materials in studies of volunteer fire departments in the future.

• The Journal of Sustainable Design and Educational Environment Research
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• v.16 no.2
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• pp.19-33
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• 2017

The purpose of this study is to derive education programs for safety training class, create unit spaces and present components and methods of utilizing the spaces for the development of facilities models closely related to various policy, operation plan and facility construction projects promoted by related institutions such as the Ministry of Education, schools, architects and companies. This study is divided into five steps. First, we reviewed the literature related basic directions for safety education and facility plan, second, field survey included both field conditions such as spatial size and facility configuration and analysis of operating conditions like hours of operation and personnel. Base on literature review and field survey, it were used to analyze strengths and weaknesses of existing safety training classes, and five facility models was developed based on the Delphi method and expert participatory design. The result show that the facility models (drafts) of safety training class were developed as follows: (1)the facility model for traffic safety(pedestrian safety, vehicle safety, subway safety) (2)the facility model for first aid(emergency rescue, how to report) (3)the facility model for disaster safety(fire evacuation safety, life earthquake safety) (4)the facility model for elevator safety(elevator safety, escalator safety) (5)the facility model for drugs and violence safety (smoking drinking, sexual harassment safety, food safety) The safety training class can be composed by combining or separating each module according to affordable space size of each school.

• Journal of the Korean Institute of Gas
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• v.22 no.5
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• pp.1-17
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• 2018

Related incidents and accidents are frequent after 2000 years, such as the outbreak of the Taian peninsula crude oil spillage and Gumi hydrofluoric acid leakage accident. In the wake of such environmental pollution accidents, Consensus has been formed to enact legislation on liability for the compensation of environmental pollution in 2014 and the rescue, and has been in force since January 2016. Therefore, in the domestic insurance industry, the introduced environmental liability insurance system needs to be managed through the standardization formula of a new insurance model for managing the environmental risk. This study has been carried out by the emergence of a safe insurance model with a risky nature of the risk type, which is one of the services of the knowledge base. The verification of the six assurance media on the occurrence of environmental pollution such as chemical, waste, marine, soil, etc. is expressed through semantic interoperability through this possible ontology. The insurance model was designed and presented by deducing the relationship between the amount of money and the amount of money that was written in the area of existing expertise, In order to exclude the possible consequences, the concept of abstract is conceptualized in the form of a customer, and a plan for the future development of an ontology-based decision support system is proposed to reduce the cost and resources consumed every year. It is expected that standardization of the verification standard of the mass of mass will minimize errors and reduce the time and resources required for verification.

• Clinical and Experimental Reproductive Medicine
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• v.19 no.2
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• pp.133-141
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• 1992

We examined effects of co-culture with human oviduct epithelial cells (HOEC) on the development of mouse and human embryos from early embryonic· stage to late morula or blastocyst stage (LM or B). In human, embryos were transferred and pregnancy rate was investigated. The HOEC, collected from surgically removed fallopian tube, were cultured in medium-199 supplemented with 20 % fetal cord serum (FCS). The HOEC were characterized by using immunocytochemical staining with anticytokeratin antibody and then used for cultures of mouse and human embryos. Results obtained from co-culture system were as follows. Development rate of mouse embryos was improved by co-culture system at late developmental stage (p<0.025). Human supernumerary embryos remained after transfer, unsuitable for freezing because of their poor quality, were co-cultured for 72hrs. Co-culture (78.79%) or conditioned medium (78.26%) system improved the developmemt rate, significantly, in comparision with control (11.11%)(p<0.00l). Co-cultured (85.71%) human zygotes for 24hrs showed the better development rate in comparision with control (50.00%) (p<0.01). When we transferred embryos cultured with the HOEC to patients, we obtained one pregnancy. Co-cultured human zygotes for 24hrs showed the better quality and viability for the replacement in comparision with control (p<0.01). In addition, improved pregnancy rate was obtained. Our results suggest that the co-culture system can rescue early degenerating embryos by improving early development and yield a resonable number of blastocyst for the appropriate replacement. The effect provided by cultured HOEC is not species specific for the development of embryos and it can be used to overcome in vitro blocks for the development. And also the co-culture system offers the possibility to freeze embryos at blastocyst stage which is more sucessful stage for the freezing. The HOEC monolayer may provide some stimulus via specific factor, which is unknown, to the development of embryos. Our results showed that the co-culture system with HOEC can be an alternative to conventional culture system.