• Journal of Life Science
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• v.27 no.1
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• pp.32-37
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• 2017

Cyanidin-3-O-glucoside (C3G) shows anti-inflammatory and antioxidant effects; however, its effect on postprandial blood glucose levels remains unknown. Alpha-glucosidase inhibitors regulate post-prandial hyperglycemia by impeding carbohydrate digestion in the small intestine. Here, the effect of C3G on ${\alpha}-glucosidase$ and ${\alpha}-amylase$ inhibition and its ability to ameliorate postprandial hyperglycemia in streptozotocin (STZ)-induced diabetic mice were evaluated. ICR normal and STZ-induced diabetic mice were orally administered soluble starch alone or with C3G or acarbose. The half-maximal inhibitory concentrations of C3G for ${\alpha}-glucosidase$ and ${\alpha}-amylase$ were 13.72 and $7.5{\mu}M$, respectively, suggesting that C3G was more effective than acarbose. The increase in postprandial blood glucose levels was more significantly reduced in the C3G groups than in the control group for both diabetic and normal mice. The area under the curve for the diabetic mice was significantly reduced following C3G administration. C3G may be a potent ${\alpha}-glucosidase$ inhibitor and may delay dietary carbohydrate absorption.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.4
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• pp.551-559
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• 1996

To examine the effects of dietary fibers on gastrointestinal physiology, rats were fed with diets containing 10% sodium alginate,10% cellulose, or fiber-free diets for 5 weeks. The results obtained were as follows: The chronic consumption of sodium alginate induced a significant decrease in body weight gain and feeding efficiency, but a significant increase in length and weight of small intestine. Fecal bulk and weight were higher in fiber-fed group than fiber-free group. The chronic consumption of dietary fiber induced a significant increase in fecal output, resulting in tile decrease of apparent digestibility of protein and lipid. Pancreatic protease activity was lower in fiber-fed group than fiber-free group, whereas pancreatic amylase and lipase activities were not affected. Scanning electron microscopy(SEM) and light microscopy(LM) studies showed small intestine microvilli with numerous ridges and convolutions and goblet cells in fiber-fed groups. As a result of this study, it is concluded that the chronic consumption of dietary fiber decreases apparent digestibility of nutrients and induces morphological and biochemical adaptation of digestive organs.

• Archives of Plastic Surgery
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• v.32 no.3
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• pp.369-375
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• 2005

Apoptosis is a physiologic or programmed cell death process which is controlled by genes. It is essential for the function and the appropriate development of multicellular organism. It is also thought to be one of the main mechanisms of cell death in ischemic tissues. The effect of prostaglandin $E_1$($PGE_1$) is proven to be useful in the recovery of ischemic changes by inducing vasodilation of peripheral vessels and platelet disaggregation. $PGE_1$ is also known to suppress apoptosis in human liver sinusoidal endothelial cell from ischemia-reperfusion injury. The purpose of this study is to evaluate the effects of $PGE_1$ on the apoptosis in the ischemia reperfusion injury of rat intestine. Thirty Sprague-Dawley rats were used. In control group(N=15), superior mesenteric artery was occluded for 60 minutes and after removing the vessel clamp, it was reperfused for 60 minutes and harvested. In experimental group(N=15), a jejunal flap was also made as in the control group except for the intraarterial administration of the $PGE_1$ right after clamping the artery and removing the clamp. H&E, TUNEL and immunohistochemical stains for p53, bax, and bcl-2 were performed. There were ischemic changes in gross and microscopic findings in both groups. The apoptotic index was significantly lower in the experimental group($1.29{\pm}0.82$(p=0.003)) than in the control group ($2.33{\pm}0.95$). The rat intestinal ischemia apoptosis by ischemia-reperfusion was partly related to the modulating of bcl-2, bax, and p53 expression. Our results indicate that $PGE_1$ suppresses the apoptosis in the ischemic jejunal flap and this effect is probably the result of a increase in expression of bcl-2.

• Parasites, Hosts and Diseases
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• v.36 no.4
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• pp.217-225
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• 1998

A scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of Metagonimus miyatai. Metacercariae were collected from the scale of the pale chub (Zacco platypus). and adult flukes were harvested 1-4 weeks after infection to rats. In excysted metacercariae, the oral sucker was devoid of tegumental spines and had type I and type II sensory papillae. Anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whereas near the posterior end of the body spines were sparse and digitated into 2-3 points. In one-week adults, 7 type II sensory papillae were arranged around the lip of the oral sucker. and at inner side of the lip one pair of small and two pairs of large type I sensory papillae were seen on each side. The distribution of tegumental spines was similar to that of metacercariae, but they were more differentiated with 9-11 pointed tips. In two- to four- week old adults, the surface ultrastructure was nearly the same as in one-week old adults, however, sperms were frequently seen entering into the Laurer＇s canal. Conclusively, the surface ultrastructure of M. miyatai was generally similar to that of M. yokogawai, however, differentiation of tegumental spines and distribution of sensory papillae around the oral sucker were different between the two species. which may be of taxonomic significance.

• Parasites, Hosts and Diseases
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• v.34 no.1
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• pp.1-6
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• 1996

Mccroorchis spinuLosw Ando, 1918 (Digenea: Nanophyetidael is redescribed based on adult flukes recovered from experimental mice, rats and cats which were fed with metacercariae encysted in the muscle of the crayfish, Cambnroines similis. A total of 117 adult flukes were harvested from the small intestine of the experimental animals at S-21 days post-infection. The worm recovery rate was 31-37%, not significantly different among the three kinds of animals. The metacercariae were round, 0.18 mm in average diameter, encysted with two layers of thick walls, and possessing a stylets on the oral sucker and Y- shaped exc retory bladder. The adult flukes were oval, 0.66-0.71 mm long and 0.36-0.41 mm wide, and characterized by the presence of a stylet on the oral sucker, variable location of the ovary near the mid-portion of the body, bilobed seminal vesicle, and anterolateral location of two testes. These morphological features were a little different from the original description, hence, an emended version of M. spinulosw was given. M. spinuLosw is a new intestinal trematode of animals and possibly man in Korea.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.2
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• pp.271-276
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• 2002

We have investigated the intestinal functions and hypolipidemic effects of resistant starch (RS) in rats. Experimental groups were CON (cooked starch 45% + RS 7%), RSIO (cooked starch 35% +RS 10%), RS20 (cooked starch 25%+RS20%), and RS30 (cooked starch 15%+RS 30%). The weight gains during experimental period were slightly decreased by intake of resistant starch and the weights of epididymal fat pad were lower in resistant starch intake groups than in CON, although the difference was not significant. In intestinal functions, water contents of fecal, transit time and colon cell proliferation were affected by resistant starch. Plasma total lipid and triglyceride concentrations were significantly decreased, dose-dependently, by resistant starch intake. Conclusively, it is important to intake resistant starch in order to decrease plasma lipids and to improve intestinal functions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.6
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• pp.519-526
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• 1991

This study was designed to investigate the effects of dietary glycosyl-sucrose on blood glucose and serum lipids concentrations and carbohydrate hydrolysing enzyme activities in rats. Male Sprague-Dawley rate were divided into 3 experimental groups, cornstarch group(68% cornstarch diet), sucrose group (20% sucrose + 48% cornstarch diet) and glycosyl-sucrose group(20% glycosyl sucrose + 48% cornstarch diet). After 6 weeks, the mean values of food efficiency ratio of 3 groups were not significantly different. There were no significant differences in mean fasting blood glucose levels of three groups, while the glucose response at 30 and 60 minutes and the group as compared with sucreose area showed a tendency to decrease in glycosyl-sucrose group as compared with sucrose group. Serum lipids (T. G., phospholipids, HDL-cholesteol and total lipid) contents showed no significant differences among 3 groups but a tendency of jejunum and ileum were significantly reduced in glycosyl-sucrose group as compared with cornstarch group. The maltose hydrolysing enzyme activity, especially in jejunum was lower in glycosyl-sucrose group than in sucrose group.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1332-1340
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• 2009

Moxa-combustion therapy makes use of heat stimulus and chemicals result form when cauterize the skin with moxa cones to medical cares. Despite an extensive evidence-base guiding the selection of stimulation parameters and attributes of subject, little methodologically research regarding the attributes of moxa cautery in need to provide effective stimulation. To determine moxibustion's effects of the three different moxa cautery made by three different company(as A, B, C), the small intestinal motility in rats were observed after moxibustion at ST36. Under anesthesia, each intensities(1, 5, 10 times), two regions(left and right at ST36) and a quantity(as number of 1, 5, 10 times at once) were applied to the groups divided with sex. In each intensities groups, the A product made increase with 5, 10 times in male and female group. The B and C product made increase with 10 times in male group and the B product made increase with 1, 5, 10 times and the C product made increase with 5 times in female group. In two regions(left and right at ST36) groups, the A and C product made increase with 1(right), 5(right) times and the B product made increase with 1(left), 10(left), 10(right) times in male group. In female group, the A product made increase with 5(left), 5(right), 10(right) times and the B product made increase with 1(left), 10(left), 10(right) times and the C product made increase with 5(left), 10(left) times. In a quantity(as number of 1, 5, 10 times at once) groups, the A and B product did not show any changes but the C product made increase with a quantity of 10 times in male group. In female group, The B product made increase with a quantity of 1 times and the C product made increase with a quantity of 5 times, but the A product did not show any changes. Three different moxa cautery made by three different company made differents result in each group divided with sex. With these results, it was suggested that we should consider the a process of manufacture and moxa cautery's quality for the adequate value of moxibustion.

• Journal of the Korean Society of Radiology
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• v.14 no.5
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• pp.553-561
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• 2020

In this study aims to investigate the radiation protection effect of avocado peel extracts on the Sprague-Dawely rats. 52 male rats were randomly classified into 4 groups. NC Group was a normal control group, PA Group was a group injected avocado peel extracts, IR Group was irradiated group, and lastly PA+IR Group was set as an irradiated group after injected of avocado peel extracts. Avocado peel extract was administered orally at 200 mg/kg once a day for 14 days before irradiation, and the radiation dose was systemically irradiated with 6 MV X-ray of 7 Gy. On the 4 and 21 days after irradiation, the experimental animals were sacrificed to evaluate the change in blood cell composition, spleen index, and histopathological evaluation of the liver and small intestine. As a result, the PA+IR Group showed a significantly greater recovery of lymphocytes(p<0.01), red blood cells(p<0.01), and platelets(p<0.05) than the IR Group. It was also confirmed that the activation of Superoxide Dismutase(SOD) was further increased. Histopathologically, observed that nuclei aggregation and cytoplasmic expansion were slightly reduced in the PA+IR Group in the liver. and the damage was significantly reduce(p<0.01) in the change of villi length due to damage to the small intestine cells. Based on the above results, avocado peel extract can be expected to act as a radiation protection agent that can reduce damage to blood cells and major organs caused by irradiation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.1
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• pp.13-20
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• 2005

The effects of pectin on obesity was studied using 3T3-L1 pre-adipocytes and rats fed 20% high fat diets. The concentration of leptin released from 3T3-L1 adipocytes in the presence of pectin was significantly decreased by 85% compared to that of the control (p<0.05), however, glycerol concentration was not changed. These data indicate that pectin seems to inhibit lipids accumulation in the adipocytes rather than enhance the lipolytic activity. Forty Sprague Dawley rats were fed 20% high fat diet for 8 weeks to induce obesity and then divided equally into four groups. Experimental groups were normal diet group (ND), high fat diet group (HFD), HDF with 10% pectin group (HFP10), and HDF with 20% pectin group (HFP20). Diet for the each group was prepared to be iso-caloric following AIN-76 guideline. After obesity was induced, rats were placed on an restricted diet for 9 weeks. The body weight of HFD increased 50% (p<0.05) compared to the ND, while it was decreased by 12% and 16% for HFP10 and HFP20, respectively (p<0.05). The relative amount of visceral fats for HFDl0 and HFD20 were decreased by 45% and 59% compared to that of HDF (130%), respectively (p<0.05). Pectin seems to have a greater effect on reducing visceral fats accumulation than weight reduction. Significantly increased level of triglyceride, total cholesterol or LDL-cholesterol in the plasma of HFD was returned to the normal or even below the normal by pectin diet, while the level of HDL-cholesterol increased. Lipid lowering effect was also observed in the liver and heart. These effects of pectin were dosedependent. In conclusion, the beneficial effect of pectin on the obesity was observed from cell culture experiment and animal study in terms of inhibiting the accumulation of lipids in the adipocytes.