Present work was executed to evaluate effects of adjuvants. stabilizers. moisture. pH and heavy metals on the stability of Fenitrothion in the emulsifiable concentrate. In addition, susceptibility ' of Fenitrothion in various formulations, to UV-irradiation has been also examined. The results are summarized as follows; 1. Xylene and benzene were found to be satisfactory solvents for Fenitrothion emulsifiable concentrate. As expected, polar sol vents such as aliphatic alcohols considerably reduced stability of the pesticides. 2. Of the two non-ionic emulsifiers, an alkyl aryl type Sorpol-1200, in contrast to sorbitan type Tweens, substantially reduced decomposition of Fenitrothion in the emulsifiable concentrates. Moisture and pH of emulsifiers. in the ranges studied. affected little if any. on the stabi ity of the Fenitrothion during the experiment periods. 3. Maleic anhydride, p-toluene sulfonic acid, sulfosalicylic acid, maleic anhydride-sulfosalicylic acid reduced decomposition of Fenitrothion in the emulsifiable concentrate. Addition of organic acids, however, increased liability of Fenitrothion in the emulsifiable concentrate. 4. Presence of either zinc or copper metals in the emulsifiable concentrate containing Tween-80 as a emulsifier, reduced stability of the Fenitrothion. 5. UV-irradiation, as expected, brought decomposition of Fenitrothion. The liability of Fenitrothion formulations decreased in the order, wettable powder ${\gg}$ dust > emulsifiable concentrate.
Park, Byung-Bae;Byun, Jae-Kyung;Kim, Woo-Sung;Sung, Joo-Han
Journal of Korean Society of Forest Science
/
v.99
no.1
/
pp.85-95
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2010
The purpose of this study was to quantitatively measure both growth performances and nutrient responses of Fraxinus rhynchophylla, Fraxinus mandshurica, Pinus koraiensis, and Abies holophylla seedlings, which are commercially planted in Korea, to nitrogen, phosphorus, and potassium fertilization. We used Dickson's quality index (QI) to compare growth performances and vector diagnosis to interpret nutrient status. Nitrogen fertilization increased more height and root collar diameter growth in F. rhynchophylla and F. mandshurica relative to no fertilization treatment. The QI of F. rhynchophylla and F. mandshurica was the highest on N treatment, but there were no significant differences between treatments for P. koraiensis and A. holophylla. Nitrogen fertilization increased total dry weight by 43, 41, 26, -9% for F. rhynchophylla, F. mandshurica, P. koraiensis and A. holophylla, respectively. In F. rhynchophylla, N fertilization increased N contents with similar N concentrations ("sufficiency"), decreased both P concentrations and P contents ("antagonism"), and decreased K contents with similar K concentrations ("toxic accumulation"). In P. koraiensis, N fertilization decreased N, P, and K concentrations because of more dry weight increases compared to uptaken contents ("dilution"), but N fertilization decreased N, P, and K contents with similar N, P, and K concentrations ("toxic accumulation"). In the light of quality index and vector diagnosis, F. rhynchophylla and F. mandshurica seedlings treated with N fertilization would have high field performance.
Purpose: The incidence rate and the mortality rate of gastric cancer have decreased in developed countries over the last several decades. On the other hand, they remain high in far eastern countries such as Korea, Japan, China and in many developing countries. The cure of patients with gastric carcinomas can be achieved mostly through complete surgical resection, but most gastric cancer patients are in advanced stages when diagnosed and have poor prognoses. therefore, the development of an effective systemic therapy is essential for far advanced gastric cancer patients. Until recently, the most commonly used combination chemotherapy was based on 5-flurouracil or cisplatin, but the results were not satisfactory, so recently etoposide, adriamycin and cisplatin (EAP-II) combination chemotherapy was introduced in patients with advanced gastric cancer. Early studies showed a high response rate and the ability to convert unresectable cases to resectable ones, but later studies couldn't duplicate the result. the purpose of this study was to evaluate the relative efficacy & toxicity of EAP-II chemotherapy and ELF chemotherapy which is based on 5-flurouracil. Materials and Methods: Between July 1992 and July 2002, sixty-five patients with inoperable advanced gastric cancer were enrolled for this study. Thirty-seven patient received EAP-II chemotherapy:etoposide (20 mg/$m^{2}$ IV for $1\∼5 days$), adriamycin (20 mg/$m^{2}$ IV for $1\∼5 days$) and cisplatin (20 mg/$m^{2}$ IV for $1\∼5 days$) and Twenty-eight patients receieved ELF chemotherapy : etoposide (100 mg/$m^{2}$ IV for $1\∼3 days$), leucovorin (20 mg/$m^{2}$ IV for $1\∼5 days$) and 5-FU (500 mg/$m^{2}$ IV for $1\∼5 days$). Each treatment schedule for each group was repeated every four weeks: EAP-II means 3.4 cycles per patient..ELF means 4.1 cycles per patient Results: Total respones rates were $5.4\%$ in the ELF group and $3.6\%$ in the EAP group (P-value>0.05). The median times to progression were 144 days in the ELF group and 92 days in the EAP-II group (P-value<0.05), and themedian overall survival times were 189 days in the ELF group and 139 days in the EAP-II group (P-value>0.05). The difference in the survival curves for the two regimens was not statistically significant. Non-hematologic toxicitis & hematologic toxicitis were more frequently observed for the EAP-II regimen. Anemia: $27.6\%$ in ELF vs $54\%$ in EAP-II; Leukopenia: $8.5\%$ in ELF vs $19\%$ in EAP-II; nausea & vomiting: $45.9\%$ in ELF vs $67.8\%$ in EAP-II. Conclusion: EAP-II regimen is not superior to ELF regimen in the tratment of inoperable advanced gastric cancer (J Korean Gastric Cancer Assoc 2003;3:122-127)
Porcine epidemic diarrhea virus (PEDV) is an infectious and highly contagious virus of swine. In order to develop the transgenic tobacco culture cells producing PEDV antigen protein, four vectors expressing PEDV spike protein (SP) gene under the control of a CaMV 35S promoter were constructed. Four fragments of the SP region of PEDV, SP1 (444 bp, 1487-1930 bp), SP2 (1.7 kb, 2300-3987 bp), SP3 (1.4 kb, 1559-2950 bp), and SP4 (2.6 kb, 9-2643 bp) were amplified by PCR and then C-MYC tag was fused to the end of each SP gene, respectively. These cassettes are inserted into the pCAMBIA2300 (named as 35S::SP1-M, 35S::SP2-M 35S::SP3-M, and 35S::SP4-M, respectively). Tobacco (cv. BY-2) cultured cells were transformed by co-cultivation with Agrobacterium tumefaciens harboring expression vector. We selected kanamycin-resistant calli and checked for the presence of the introduced SP gene using PCR, resulting 70% of them showed the foreign gene. We selected the lines with high-level expression of PEDV antigen protein based on dot blot analysis. Southern blot analysis confirmed that the PEDV SP gene was integrated into the genome of the tobacco cultured cells. Northern blot analysis showed that the introduced gene was highly expressed in transgenic cultured cells. Transgenic tobacco cultured cells-derived antigen induced immunogenicity in mice as determined by a plaque reduction neutralization assay. These results suggest that the vectors expressing PEDV spike protein gene in this study will be useful for the development of transgenic plants and cultured cells producing PEDV antigene protein.
Effects of fungicides on the mycelial growth of Botrytis cinerea isolated from ginseng leaves were investigated by an agar dilution method. By using a agar dilution method, it was investigated the effect of fungicides, procymidone, carbendazim and the mixture with both of carbendazim and diethofencarb, on the mycelial growth of Botrytis cinerea isolates, which were isolated from infected leaves of ginseng in 2005 and 2006. With MIC (minimum inhibiton concentration) of procymidone against B. cinerea, pathogens were divided into two groups. While one showed the low MIC between 0.8 and $4.0{\mu}g/ml$, the other showed higher MIC above $20{\mu}g/ml$. In terms of the inhibition ratio of mycelial growth at the indicated concentration of procymidone, isolates of B. cinerea were divided into three groups; the sensitive, the intermediate resistant, and the resistant group. Each group was differentiated by $EC_{50}$; the sensitive group showed below $2.0{\mu}g/ml$, the intermediate resistant group between 2.0 to $5.0{\mu}g/ml$, and resistant group above $5.0{\mu}g/ml$. Compared with the ratio of resistant isolates of B. cinerea in 2005, the ratio in 2006 increased from 19.3% to 27.5%. Furthermore, the average $EC_{50}$ value of them increased from $10.0{\mu}g/ml$ in 2005 to $237.3{\mu}g/ml$ in 2006. The ratio of isolates showing the multiple resistance between procymidone and carbendazim was 40.2%, whereas the ratio was 4.0% showing the multiple resistance in the mixture.
The objective of this study was to analyze temporal trends of water chemistry and spatial heterogeneity between the dam site (Daecheong Reservoir, S1) and the downstream (S2$\sim$S4) using water quality dataset (obtained from the Ministry of Environment, Korea) during 2000$\sim$2007. Water quality, based on eight physical and chemical parameters, varied largely depending on the years, sampling sites, and the discharge volume. Conductivity and nutrients (TN and TP) showed a decreasing trend in the downstream (S4) rather than the dam site during the monsoon. Spatial variation increased toward downstream (S4) from Daecheong Reservoir (S1). Also, BOD and COD increased toward downstream. Because of input of nutrient and pollutant nearby S1, lentic ecosystem in monsoon, BOD and COD were slightly increased. whereas relatively decreased in S4, lotic ecosystem in monsoon, by dilution effect of nutrient and pollutant by discharge from upper dam, S1. Spatial variation of SS increased toward downstream (S4) by the side of Daecheong Reservoir (S1). Based on the dataset, efficient water quality management in the point source tributary streams is required for better water quality of downstream. Monthly characteristics of DO showed the lowest value in the monsoon that tend to increase water temperature. DO was lowest in October at S1 because turbid water, input to the Daecheong Reservoir in the monsoon affect to the postmonsoon period. In contrast, water temperature increased toward summer monsoon, in spite of some differences showed between S1 and S4 environment. Overall, the characteristics of water quality in downstream region have close correlation with discharge amount of Daecheong Reservoir. Thus, those characteristics can explain that discharge control of upper dam mainly affect to the water quality variation in downstream reach.
Purpose : The patients who visited this department for pulmonary disease and need CT scans for Follow-up to observe change of CT value, evaluation of image quality and decrease of radiation dose as change of kVp. Subjects and Methods : Subjects were the patients of 20 persons visited this department for pulmonary disease and Somatom Sensation 16(Semens, Enlarge, Germany) was used. Measurement of CT value as change of kVp was done by setting up ROI diameter of 1cm at the height of thyroid, aortic arch, right pulmonary artery in arterial phase image using 100 kVp, measuring 3 times, and recorded the average. CT value of phantom was measured by scanning phantoms which means contrast media diluted by normal saline by various ratio with tube voltage of 80 kVp, 100 kVp, 120 kVp, 140 kVp and recorded the average of 3 CT values of center of phantom image. In analysing radiation dose, CTDIVOL values of the latest arterial phase image of 120 kVp and as this research set that of 100 kVp were analyzed comparatively. 2 observers graded quality of chest images by 5 degrees (Unacceptable, Suboptimal, Adequate, Good, Excellent). Results : CT value of chest image increased at 100 kVp by 14.06%~27.26% in each ROI than 120 kVp. CT value of phantom increased as tube voltage lowered at various concentration of contrast media. CTDIVOL decreased at 100 kVp(5.00 mGy) by 36% than 120 kVp(7.80 mGy) in radiation dose analysis. here were 0 Unacceptable, 1 Suboptimal, 3 Adequate, 10 Good, 6 Excellent in totally 20 persons. Conclusion : Chest CT scanning with low kilo-voltage for patients who need CT scan repeatedly can bring images valuable for diagnose, and decrease radiation dose against patients.
Kim, Su-Zy;Kwon, Eun-Kyung;Lee, Seung-Hee;Park, Hye-Jin;Wu, Hong-Gyun
Radiation Oncology Journal
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v.25
no.4
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pp.227-232
/
2007
Purpose: To investigate the flavopiridol effect on radiation-induced apoptosis and expression of apoptosisrelated genes of human laryngeal and lung cancer cells. Materials and Methods: A human laryngeal cancer cell line, AMC-HN3 and a human lung cancer cell line, NCI-H460, were used in the study. The cells were divided into four groups according to the type of treatment: 1) control groups; 2) cells that were only irradiated; 3) cells treated only with flavopiridol; 4) cells treated with flavopiridol and radiation simultaneously. The cells were irradiated with 10 Gy of X-rays using a 4 MV linear accelerator. Flavopiridol was administered to the media at a concentration of 100 nM for 24 hours. We compared the fraction of apoptotic cells of each group 24 hours after the initiation of treatment. The fraction of apoptotic cells was detected by measurement of the sub-G1 fractions from a flow cytometric analysis. The expression of apoptosis-regulating genes, including cleaved caspase-3, cleaved PARP (poly (ADP-ribose) polymerase), p53, p21, cyclin D1, and phosphorylated Akt (protein kinase B) were analyzed by Western blotting. Results: The sub-G1 fraction of cells was significantly increased in the combination treatment group, as compared to cells exposed to radiation alone or flavopiridol alone. Western blotting also showed an increased expression of cleaved caspase-3 and cleaved PARP expression in cells of the combination treatment group, as compared with cells exposed to radiation alone or flavopiridol alone. Treatment with flavopiridol down regulated cyclin 01 expression of both cell lines but its effect on p53 and p21 expression was different according to each individual cell line. Flavopiridol did not affect the expression of phophorylated Akt in both cell lines. Conclusion: Treatment with flavopiridol increased radiation-induced apoptosis of both the human laryngeal and lung cancer cell lines. Flavopiridol effects on p53 and p21 expression were different according to the individual cell line and it did not affect Akt activation of both cell lines.
Immune response to two methods of Newcastle disease virus vaccine, one inactivated and the other attenuated was observed and the data presented. (1) Administration of inactivated virus vaccine in an amount of 1.0 ml. by intramuscular route gave an appreciable immunity to Newcastle disease for a period of at least three-and-half months. (2) The chickens given attenuated virus vaccine in the drinking water produced satisfactory immunity as manifested by the fact that immunized birds showed resistance when challenged 105 days after the vaccination and maintained high degree of HI titer for a period of 75 days. (3) Vaccination with the attenuated virus vaccine in drinking water is very simple and time saving in procedure, although the duration of immunity seems to be slightly shorter than that proced by inactivated virus vaccine. The author wishes to express his appreciation, to Drs. Kyu Myung Lee, Chang Koo Lee, and Ryong Sook Kee of their suggestions and help. The author is also indebted to Dr. Chang Hi Lee, Director of Anyang Veterinary Laboratory, who allowed the use of the facilities of the laboratory, whitout which this experiment could not have been undertaken.
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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v.4
no.1
/
pp.71-79
/
1999
As part of an on-going project investigating flux of materials in the Keum River Estuary, we have monitored seasonal variations of nutrients, suspended particulate matter (SPM), chlorophyll, and salinity since 1997. Meteorological data and freshwater discharge from the Keum River Dike were also used, Our goal was to answers for (1) what is the main factor for the seasonal fluctuation of nutrients in the Keum River Estuary? and (2) are there any differences in nutrient distributions before and after the Keum River Dike construction? Nitrate concentrations in the Keum River water were kept constant through the year. Whereas other nutrients varied with evident seasonality: high phosphate and ammonium concentrations during the dry season and enhanced silicate contents during the rainy season. SPM was found similar trend with silicate. During the rainy season, the freshwater discharged from the Keum River Dike seemed to dilute the phosphate and ammonium, but to elevate SPM concentration in the Keum Estuary. In addition, the corresponding variations of SPM contents in the estuarine water affected the seasonal fluctuations of nutrients in the Estuary. The most important source of the nutrients in the estuarine water is the fluvial water. Therefore, the distribution patterns of nutrients in the Estuary are conservative against salinity. Nitrate, nitrite and silicate are conservative through the year. The distribution of phosphate and ammonium on the other hand, display two distinct seasonal patterns: conservative behavior during the dry season and some additive processes during the rainy days. Mass destruction of freshwater phytoplankton in the riverine water is believed to be a major additive source of phosphate in the upper Estuary. Desorption processes of phosphate and ammonium from SPM and organic matter probably contribute extra source of addition. Benthic flux of phosphate and ammonium from the sediment into overlying estuarine water can not be excluded as another source. After the Keum River Dike construction, the concentrations of SPM decreased markedly and their role in controlling of nutrient concentrations in the Estuary has probably diminished. We found low salinity (5~15 psu) within 1 km away from the Dike during the dry season. Therefore we conclude that the only limited area of inner estuary function as a real estuary and the rest part rather be like a bay during the dry season. However, during the rainy season, the entire estuary as the mixing place of freshwater and seawater. Compared to the environmental conditions of the Estuary before the Dike construction, tidal current velocity and turbidity are decreased, but nutrient concentrations and chance of massive algal bloom such as red tide outbreak markedly increased.
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