• Korean Journal of Food Science and Technology
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• v.41 no.6
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• pp.687-693
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• 2009

This study was conducted to investigate the antioxidant activity, fibrinolytic activity and cytotoxic effect of Korean traditional noble rice wine made using different methods (A-C) and commercial rice wine (D-H) on various tumor cell lines. The antioxidant activity of rice wine was measured by DPPH (2,2-dipicryl-1-picrylhydrazyl), ABTS [2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid)] and NO (nitric oxide) radical scavenging assay. In this study, Samhaeju showed the greatest fibrinolytic activity of 13-17U and exhibited the highest antioxidant activity. Among the different Samhaeju, the sample prepared using method C had the highest antioxidant activity. The cytotoxic effect of rice wine were also examined against the human cancer cell line (A549 cells and HeLa cells) based on the results of a WST-1 assay and morphological changes. Rice wine induced the inhibition of cell proliferation and morphological changes in tumor cell lines in a concentration-dependent manner, with Samhaeju diluted 10 fold having the strongest effect on these factors. These findings suggest that Korean rice wine has antioxidant activity and cytotoxic effect, and that these factors are influenced by the method of preparation.

• Research in Plant Disease
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• v.16 no.1
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• pp.27-34
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• 2010

This study was performed to develop a formulation using an antagonistic bacterium Bacillus amyloliquefaciens A-2 to control tomato leaf mold caused by Fulvia fulva. B. amyloliquefaciens A-2 was grown in a medium with rice oil and mixed with various carrier and additives. One of the formulations, A2-MP, showed the best disease control value among the tested formulations. The disease control value of A2-MP at 100-fold and 500-fold diluted treatment was not significantly different from that of chemical fungicide triflumizole in a growth chamber. Although disease control effect was decreased by serial diluted treatment of the prepared A2-MP, 1,000-fold diluted treatment of A2-MP still showed high disease control value of 72.0%. For the green house experiments, the disease control values of A2-MP was indicated as 79.4% which is similar to that of chemical fungicide, triflumizole showing 79.6%. When the disease control activity of the formulation A2-MP was compared in tomato production conditions, disease control values of 100-fold diluted A2-MP and 3,000 fold diluted triflumizole exhibited 60%, 81.6%, respectively. The disease control efficiency by A-2MP was 73% of the disease control value of chemical fungicide. The formulation A-2MP maintained the stable bacterial viability and disease control activity when stored at $4^{\circ}C$. This result suggested that A-2MP develped from B. amyloliquefaciens A-2 could be used to control tomato leaf mold.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.2
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• pp.211-215
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• 1997

Experiments were performed to find out the proper conditions of diluents for the short-tenn storage of sperm of black seabream, Acanthopagrus schlegeli. For milt collection, brood stock was reared in a recirculating seawater system. The following results indicated that short-term storage methods with fresh condition could tie employed in black seabream sperm. When the black seabream's serum was used as a diluent for the fresh storage, sperm activity index (SAI), fertilization rate and survival rate derived the best result in $7\~10\;days$ of storage. pH 7 and 8 showed the highest SAI in the same storage condition. In order to keep high SAI and survival rate of the sperm, addition of 800 ppm neomycine into the diluent revealed the best storage results.

• Journal of the Korean Vacuum Society
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• v.7 no.1
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• pp.29-34
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• 1998

The solid-phase crystallization (SPC) of plasma-enhanced chemical vapor depsoited (PECVD) amorphous silicon (a-Si) films ha s been investigated by x-ray diffraction (XRD). The a-Si films were prepared on Si (100) wafers using $SiH_4$ gas and without $H_2$ dilution at the substrate temperatures between $120^{\circ}C$ and $380^{\circ}C$, and than annealed at $600^{\circ}C$ for crystallization. The annealed samples exhibited (111), (220), and (311) XRD peaks with preferential orientation of (111). The XRD peak intensities increased as the substrate temperature decreased, and the $H_2$dilution suppressed the solid-phase crystallization. The average grain size estimated by XRD analysis for the (111) texture has increased from about 10 nm to about 30 nm, as the substrate temperature decreased. The deposition rate also increased with the decreasing substrate temperature and the grain size was closely dependent on the deposition rate of the films. The grain size enhancement was attributed to an increase of the structural disorder of the Si network.

• Journal of Life Science
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• v.23 no.1
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• pp.79-83
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• 2013

A biofertilizer, BIOACTIVE, was manufactured by N-acetyl-thioproline (ATCA) and mineral phosphate solubilizing bacteria. The growth promoting effect of the biofertilizer on lettuce was evaluated under three different pot conditions, and its stability was assessed in the field. According to the results of the pot experiments, plant growth was improved compared with that of control: 128%, 122%, and 153% for the leaf number, leaf length, and leaf mass, respectively. Applying the manufactured biofertilizer increased the concentration of phosphate: 118% and 132% in the cultivation soil and plant cells, respectively. These show that BIOACTIVE may have potential as an effective biofertilizer in agriculture.

• Korean Journal of Food Science and Technology
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• v.36 no.5
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• pp.812-817
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• 2004

Cytotoxic effects of Korean rice-wine (Yakju) made with different processes and ingredients (Korean rice-wines I, II), red wine, white wine, beer, and Japanese rice-wine (Sake) were examined against human cancer lines (DLD-1, HepG2, K562) and mouse cancer lines (EMT6, LLC1). Red wine showed cytotoxic effect on all cancer lines, while Korean rice-wines I, and II showed cytotoxcity on all cancer cells except DLD-1. White wine, beer, and Japanese rice-wine had no or little cytotoxic effect against all cancer cell lines. Concentrate of Korean rice-wine only showed cytotoxic effect against DLD-1. These results suggest Korean rice-wine has strong anti-cancer effects, which are induced by certain rice-wine components.

• The Korean Journal of Mycology
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• v.44 no.3
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• pp.176-183
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• 2016

Bacillus velezensis CC112 inhibited the mycelial growth of several plant pathogens, including Rhizoctonia solani, causing damping-off on ginseng. The control efficacies of B. velezensis CC112 against R. solani by seed dipping in LB and BSM broth diluted 10 times, soil dipping, and soil drenching with LB broth diluted 10 times were 65.8%, 67.1%, and 64.2%, respectively. Treatment of soil drenching with the 100 times diluted prototype of B. velezensis CC112 against R. solani and Pythium sp. by soil revealed control efficacies of 77.3% and 65.7%, respectively. These results indicate that B. velezensis CC112 is a prospective biofungicide for the biological control of ginseng damping off.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.91-91
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• 1997

흰쥐의 뒷발바닥에 carragennan(cg)을 피하투여하여 염증성 통증을 유발한 뒤, 그 통증이 최고에 달하는 3시간 뒤 Ach이나 SNP를 동일한 항법으로 투여해서 30분 후 cg유발 통증에 대하여 그 효과를 Randall-Selitto paw pressure test로 검색하였고, 체내 Nitric Oxide Synthase(NOS) 억제제인 L-NAME과 L-NOARG를 용량별로 적용하여 Ach의 진통효과를 억제하는 정도와 Methylene Blue 및 Hemoglobin을 투여해서 SUP효과 억제를 검사하였다. 그리고 척수 지주막하강내로 catheter를 삽입하여 위의 NO donor를 주입하고, NO의 중추신경계에서의 통각전도에 미치는 효과를 Tail-Flick test로 살펴보았다. 끝으로 NO가 가진 급,만성 통각관련효과를 희석한 formalin을 써서 검색하였다. 결 과: Ach과 SNP는 흰쥐에게 말초경로투여시 유의성있는 진통효과를 보였으며, NOS inhibitor와 NO scavenger로 그 효과가 역전되었다. 또한 NOS inhibitor간에는 억제효과가 용량의존성이 유사하게 나타났고, 중추신경계로의 직접투여때는 여러 생리적 조건이 직, 간접으로 관여함이 확인되었다.

• 농업기술회보
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• v.49 no.2
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• pp.33-34
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• 2012

벼 무논점파란 무논상태에서 종자를 점파형식으로 파종함으로써 파종의 균일도를 높이고, 입모 및 벼 생육이 고르게 자랄 수 있게 한다. 따라서 벼 직파를 하되, 입모 및 벼의 생육을 안정적으로 도모하여 마치 기계이앙으로 재배한 벼와 비슷한 효과를 보이는 벼 무논점파 재배기술을 농가현장에서 실증한 결과와 농민방응 및 기술보급 경향을 알아보자.

• Proceedings of the Korea Society for Simulation Conference
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• 2000.11a
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• pp.125-130
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• 2000

침입 탐지 시스템이 침입 행위를 탐지하기 위해서 시간에 대한 처리를 고려하지 않고는 침입을 탐지할 수 없는 경우(예 Denial of Service)가 존재한다. 즉 사건의 발생 시점에 대한 처리없이는 침입 탐지가 불가능하다. 본 논문에서는 시뮬레이션 모델을 통하여 시간에 관한 처리를 체계적으로 구성하고, 여러 가지 상황을 조성하여 반복적으로 실행함으로써 침입 탐지 시스템의 핵심 요소인 침입 판별을 효과적으로 수행할 수 있도록 하였다.