Kim, Ji-Ae;Ryoo, Seung-Heui;Yu, Sung-Lan;Lee, Jun-Heon;Seo, Gil-Woong;Kim, Sun-Kyun;Sang, Byung-Chan
Korean Journal of Agricultural Science
/
v.29
no.2
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pp.43-52
/
2002
This study was performed to provide the basic data for preservation and improvement of genetic resources according to finding genetic construction obtained from analysis of genetic characteristics of $\beta$-casein gene in Korean Native goat and Saanen using the PCR-RFLP. This study confirmed the amplified products of 481bp fragments obtained from the amplification of $\beta$-casein loci by PCR. The $\beta$-casein AB genotype showed 481, 284 and 197bp, and $\beta$-casein BB genotype showed 284 and 197bp fragments in Korean Native goat and Saanen. The frequencies of $\beta$-casein genotype in Korean Native goat were 6.25 and 93.75% for AA and AB and the frequencies of $\beta$-casein genotype in Saanen were 57.14 and 42.86% for AA and AB types. The frequencies of $\beta$-casein A and B alleles were 0.031 and 0.969 in Korean Native goat and the frequencies of $\beta$-casein A and B alleles are 0.286 and 0.714 in Saanen, respectively. The nucleotide sequence of $\beta$-casein gene of Korean Native goat was 97.71% higher homology with 11 nucleotide sequences difference of that of goat reported in GeneBank (M90556). Therefore, this study of molecular genetic characteristics by the analysis of genetic polymorphism and sequencing for $\beta$-casein gene should be used as basic and applying data for preservation and improvement of genetic resources in Korean Native goat breeding.
Kang, Ho Bum;Ryoo, Seung Heui;Lee, Sang Hoon;Jeon, Byung Soon;Sang, Byung Chan
Korean Journal of Agricultural Science
/
v.25
no.1
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pp.79-88
/
1998
This study was performed in order to apply to effective breeding of Korean native cattle on the molecular genetic level obtained from PCR and nucleotide sequencing analysis of BoLA DRB3 exon2 that has important roles in host immune defence. Genomic DNA used in this study was prepared from the blood of Korean native cattle in Korean Native Cattle Improvement Center of National Livestock Cooperation. The results obtained from this study are summarized as follows: 1. Genomic DNA extracted from the blood of Korean native cattle was subjected to electrophoresis on 1.5% agarose gel. Major band was bigger than 12.2kb, indicating that genomic DNA was well prepared for PCR. Amplified products of 284bp fragments was obtained the amplification of BoLA DRB3 exon2 gene by PCR. 2. Cloning of BoLA DRB3 exon2 of Korean native cattle with pCR2.1 vector was conformed by 300bp fragment from recombinent plasmid that restricted with enzyme digestion of EcoRI. 3. Homology of BoLA DRB3 exon2 alleles of parent was 82.0% between sire's alleles and 90.1% between dam's alleles. 4. In pedigree analysis using BoLA DRB3 exon2 gene, sequencing result of BoLA DRB3 exon2 genes showed inheritance by Mendelian mode through the parents to their offspring. 5. Taking together those experimental results, pedigree was confirmed on the basis of sequencing for the alleles of parents and offspring. This knowledge by the molecular biological approach could be served for the improvement of Korean native cattle.
The purpose of this study was to examine the utilization of oral health education media among dental hygienists in charge of oral health education. The findings of the study were as follows. 1. In regard to the general utilization of educational media in possession by workplace, age and career, the dental hygienists who worked in public health clinics(42.4%), who were in their 40s and up(341.%) and whose career was six years or more(32.2%) made more use of the educational media. Those who didn't use the educational media cited time constraints as the most common reason(46.1%). 2. Models(53.2%) were highly preferred in most of the institutions where the dental hygienists worked, but the public health clinics(81.8%) and university hospitals(80.0%) were most fond of video clips. 3. Concerning preference for educational media by career, the dental hygienists whose career was between three and less than six years and who had a 10 years or more of career had the most liking for video clips, followed by models. Those whose career was between one to less than three years and between six and less than 10 years showed the most preference for models, followed by video clips. 4. As for their perception of the necessity of educational media, the majority felt the need for the media(87.5%). Regarding difficulties in purchasing necessary educational media, the biggest group pointed out a shortage of information(56.1%).
About 380 dental hygienists who were working in public health centers in 2004 joined this study with questionnaires about the evaluation of job training programs regarding oral health care. The findings are as follows. 1. As for the general characteristics of subjects including location, age and career, the dental hygienists in Public Health Subcenters outnumbered the dental hygienists in Public Health Centers, as the former accounted for 61.1 and the latter 35.3 percent. And the dental hygienists in their 36~40s made up the largest age group. The group in their 11~15 years career revealed 48.4, over 16 years career 35.7, under 10 years career group 15.9 percent, respectively. 2. The portion of certification in the respondents was 28.9 percent, as compared to the 71.1percent of non-certification. The major reasons of non-certification were not-applying(44.6%), participation rejected (29.3%). 3. Freqently requested curriculums were school based oral health program(4.42, the highest by Linkert 5 point scale), followed by oral health education(4.41), public oral health services for the toddler and for the elderly(4.04), for the disabled(3.92), oral health planning evaluation(3.85) and oral health survey& investigation(3.69). The gap between Dental hygienists at Public Health Center and Public Health Subcenter was statistically significant different(p < 0.001) in oral health survey& investigation and oral health planning evaluation.
Regional disparities between eastern and western regions is the most of serious problem for balanced regional development in Turkey. The Southeastern Anatolia Project (GAP) is being implemented to eliminate these regional development disparities. The work that was initially planned as predominantly for hydraulic energy production to utilize water resources of the Tigris and Euphrates rivers more effectively was later transformed into an integrated multi-sector regional development project. This study noted that this region had very limited cash crop production because of the constraints of semi-arid climate of the southeastern region, however, later, it has changed Turkey's major cotton producing region since Southeastern Anatolia Project carried out. Therefore, this study investigated background, process, and content of the Southeastern Anatolia Project with respect to high cotton productivity in this region and examined the dynamic changes of cotton productivity in this region. In addition, Sanliurfa prefecture is one of the main development axes of the Southeastern Anatolia Project, because government investments are concentrated on this prefecture. Therefore, this study examined the background and process of cotton farming growth in this prefecture. In 2011, Sanliurfa prefecture produced 37.6% of Turkey's total cotton production. This is mainly due to agricultural infrastructure expansion such as land consolidation, irrigation, roads and farm roads. Also, it is one of the main factor that subsidies paid to farmers for cotton cultivation. The introduction of irrigation has dramatically changed the direction of seasonal migration of this area. Prior to irrigation, this area had a serious social issue about out-migration for seasonal labor to other areas. However, the introduction of irrigation made this area that changed to in-migration and intramigration for cotton cultivation. Irrigation water is supplied to farmers through the WUAs (Water User Associations) that handed over irrigation water management, operation from DSI (General Directorate of State of Hydraulic Works). However, the WUAs are under the influence of Ashiret, a traditional feudal social structure. Because of this reason, it does not have an efficient management for farmers. Also, it is one of the reasons that this area does not have autonomous farmer organization.
Song, Hyoung-Seok;Park, Young-Min;Nam, Min;Kim, Leen
Sleep Medicine and Psychophysiology
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v.7
no.1
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pp.51-59
/
2000
Objectives: It is been reported that sleep patterns change significantly during the adolescent years. In Korea, the sleep patterns of adolescents have not been investigated systematically. The authors performed preliminary study about sleep patterns of middle and high school students in Seoul before nationwide study. Methods: The authors selected one of middle and high schools located in typical middle class area in Seoul. The study was performed during the periods when examination or vacation did not affect the sleep cycle. Fifty to one hundred male and female students were selected from one school year each. One of investigators explained the purposes and methods of this study to the students and gave additional explanation to the teachers in charge. We designed a questionnaire for subjects to describe the nap time, bedtime, sleep latency, total sleep time, rise time and sleep efficiency, and the students recorded sleep logs for seven days including weekend. Eight hundred three students answered the questionnaires. Among them, we selected the subjects who recorded sleep logs at least three days and filled up the questionnaires completely. Finally, seven hundred seventy one subjects were included in this study. Weekend was defined as the time from Saturday morning to Sunday morning, and Sunday sleep patterns were treated separately. Results: Across the six school years, school-night bedtimes were delayed one hundred twenty four minutes and school-ight rise times were advanced by thirty five minutes. School-night total sleep times were decreased by one hundred fifty eight minutes, and weekend bedtimes were delayed by one hundred twenty five minutes. The students tended to oversleep on weekends, and the mean of weekend oversleep was ninety six minutes. But, bedtime in weekends was not delayed. Daytime sleep, sleep latency and sleep efficiency were not changed greatly across six school years. Total sleep time of female students was decreased significantly between third middle school year and first high school year, but time of male students was decreased between first and second high school year. Conclusion: It is supposed that sleep deficiency of middle and high school students in Seoul was due to delayed bedtimes and advanced rise times across the school years.
This study was carried out to investigate the operational time and productivity of logging operation by chain saw, yarder attached on tractor, tower-yarder, mini-truck, mini-forwarder, and chipping operations by mini-chipper, large-chipper in order to develop the efficient logging operation system for utilization of forest-biomass. As a result, the average felling and bucking time using chain saw at the site 1 and 2 was observed to be 182.7 sec/cycle and 518.5 sec/cycle respectively. The average yarding time was 202.5 sec/cycle using yarder attached on tractor and 295.1 sec/cycle using tower-yarder. The average forwarding time was 2,073 sec/cycle using mini-truck and 2,248.4 sec/cycle using mini-forwarder. The operational time of felling and bucking using chain-saw can be delayed according to the direction of fallen trees. The selection of felling direction is very important to yarding operation because the direction between width-yarding and felling are interrelated. Productivity can be improved through educating and training operators in the yarding operations. Mini-forwarder is needed to use because of higher productivity and lower cost than mini-truck. The operational productivity of felling and bucking by chain saw was $66.96m^3/man{\cdot}day$ and $43.86m^3/man{\cdot}day$ at site 1 and 2 respectively. The yarding productivity was $5.68m^3/man{\cdot}day$ by yarder attached on tractor, $10.74m^3/man{\cdot}day$ by tower-yarder. The forwarding productivity was $21.29m^3/man{\cdot}day$ by mini-truck, $28.57m^3/man{\cdot}day$ by mini-forwarder. The chipping productivity was $4.42m^3/man{\cdot}day$ by mini-chipper, $21.87m^3/man{\cdot}day$ by large-chipper.
Journal of the Korean Society of Food Science and Nutrition
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v.39
no.4
/
pp.526-534
/
2010
The prevalence rate for chronic diseases such as obesity, diabetes, hypertension etc. caused by the increment of national income and the change of food life according to the globalization in Korea have been increased. Especially excess sodium intake may contribute to the development of hypertension, increasing cardiovascular disease risk. The objective of this study was to investigate sodium intake of nursery school meals in Gyeonggi-Do, and to construct database for lesser sodium intake policy. Survey consisted of 601 sample intakes of sodium in summer and in winter. A food weighed record method was used for measuring food intakes. Average intakes of ten children per nursery school were measured. The sodium contents of meals were analyzed by ICP-OES (inductively coupled plasma-optical emission spectrometer) after acid digestion by microwave. The sodium contents on food groups showed that sources (693 mg/100 g), grilled foods (689 mg/100 g) and kimchies (643 mg/100 g) had respectively higher sodium contents and the average sodium intake per meal was $582\pm204$ mg. The sodium contents of soups & hot soups and kimchies had 37.5% and 15.8% of total sodium intakes per meal, respectively. Sodium intakes per meal in summer and winter showed 572.3 mg and 592.3 mg, respectively. Regional ranking of sodium intakes showed the ascending order of apartment (514.3 mg/meal), rural region (540.5 mg/meal), multiplex house (635.9 mg/meal) and industrial complex (696.4 mg/ meal). A habit of excessive sodium intakes in childhood will threaten their health when they grow up to be adults; thus lesser intake of sodium per meal is needed for children in nursery school.
Purpose: Circular stapled gastrectomy has been the favored procedure with its feasibility and the shortened operative time, but anastomotic leakage, stenosis and bleeding have been reported as problems. The aim of this study was to identify what can be done to supplement the safety of this technique by examining the potential complications of performing circular stapled gastrojejunosomy after radical subtotal gastrectomy. Materials and Methods: As subjects, this study selected 1,391 patients who underwent gastrojejunostomy after radical subtotal gastrectomy because of gastric cancer at our Department of Surgery from Jan. 1998 to Dec. 2007. The patients were divided into Group I (n=479) who underwent hand-sewn gastrojejunostomy, Group II (n=48) who underwent linear stapled gastrojejunostomy and Group III (n=864) who underwent circular stapled gastrojejunostomy. Group III was re-divided into two subgroups on the basis of the point of time that a visual check was intraoperatively performed at the anastomotic site: Group III-A (n=198) before and Group III-B (n=666) after. The characteristics and complications of the patients were then compared. Results: For the comparison of the complications between Group I, Group II and Group III, anastomotic leakage was found in 7 cases (1.5%) in Group I, in 1 case (2.0%) in Group II and in 10 case (1.2%) in Group III, and anastomotic stenosis were found in 4 cases (0.8%) in Group I, 1 case (2.0%) in Group II and 5 case (0.6%) in Group III. Anastomotic bleeding was found in 32 cases (6.7%) in Group I, in 5 cases (10.4%) in Group II and in 67 cases (7.7%) in Group III. For the comparison of complications between Group III-A and Group III-B, anastomotic bleeding was found in 57 cases (28.8%) in Group III-A and 10 cases (1.5%) in Group III-B and the difference was statistically significant (P=0.037). Conclusion: Circular stapled gastrojejunostomy after radical subtotal gastrectomy is recommended because of the safety and feasibility of this technique, but bleeding at the anastomotic site may be the critical issue. In conclusion, direct inspection for bleeding at the anastomotic site during the operation will improve the safety of performing circular stapler anastomosis.
Nonpoint source pollution causes leaks and overtopping, depending on the state of the sewer network as well as aggravates the pollution load of the aqueous water system as it is introduced into the sewer by wash-off. According, the need for efficient sewer monitoring system which can manage the sewage flowrate, water quality, inflow/infiltration and overflow has increased for sewer maintenance and the prevention of environmental pollution. However, the sewer monitoring is not easy since the sewer network is built in underground with the complex nature of its structure and connections. Sewer decontamination mechanism as well as pipe network monitoring and fault diagnosis of water network system on system analysis proposed in this study. First, the pollution removal pattern and behavior of contaminants in the sewer pipe network is analyzed by using sewer process simulation program, stormwater & wastewater management model for expert (XP-SWMM). Second, the sewer network fault diagnosis was performed using the multivariate statistical monitoring to monitor water quality in the sewer and detect the sewer leakage and burst. Sewer decontamination mechanism analysis with static and dynamic state system results showed that loads of total nitrogen (TN) and total phosphorous (TP) during rainfall are greatly increased than non-rainfall, which will aggravate the pollution load of the water system. Accordingly, the sewer outflow in pipe network is analyzed due to the increased flow and inflow of pollutant concentration caused by rainfall. The proposed sewer network monitoring and fault diagnosis technique can be used effectively for the nonpoint source pollution management of the urban watershed as well as continuous monitoring system.
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