• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.17 no.4
• /
• pp.271-279
• /
• 1984

Effects of temperature and additives on the stability of actomyosin extracted from skeletal muscle of Israeli carp, Cyprinus carpio nudus, were studied by analyzing free SH-group, ATP-sensitivity and Ca-ATPase activity. The used additives were sucrose, sorbitol, Na-glutamate and L-cysteine. Furthermore, the denaturation constant($K_D$), protective effect(${\Delta}E/M$) and the other thermo-dynamic parameters on protein denaturation are systematically discussed. The actomyosin showed $4.12{\sim}4.68 mg/ml$ in protein concentration, $2.63{\sim}2.93\%$ in ribonucleic acid to the protein, $1:2.20{\sim}2.63$ in the binding ratio of myosin and actin, $4.33{\sim}5.26\%$ in fat content, 109.78 in ATP-sonsitivity, $0.159{\sim}0.201\;{\mu}M-Pi/min/mg-protein$ in Ca-ATPase activity and $3.3{\sim}3.4M/10^5$g-protein in free SH-group content. The first-order rate plots were obtained on the decrease of Ca-ATPase activity and ATP-sensitivity with an increase in temperature, while the free SH-group was increased to $60^{\circ}C$ and decreased rapidly above the temperature. The half-life of Ca-ATPase activity on the actomyosin Ca-ATPase was 280 min at $12^{\circ}C$, 125 min at $20^{\circ}C$, 55 min at $30^{\circ}C$ and 13 min at $40^{\circ}C$, and activation energy, activation enthalpy, activation entropy and free energy of the proteins at $20^{\circ}C$ wene 5,395 cal/mole, 4,814 cal/mole, -40.42 e.u. and 17,626 cal/mole, respectively. The protective effect of the additives on the actomyosin Ca-ATPase showed that the most effective material is $3\%$ sorbitol and followed in the order of $8\%$ Na-glutamate, $1\%$ sucrose and $1\%$ L-cysteine. The actomyosin was more stable at $-30^{\circ}C$ than at $0^{\circ}C$ and $-20^{\circ}C$. and when the additives were used in the low temperature storage, $8\%$ Na-glutamate was the most effective. $3\%$ sorbitol, $1\%$ sucrose and $1\%$ L-cysteine was to become lower in the order.

• 한국신재생에너지학회:학술대회논문집
• /
• 2010.06a
• /
• pp.133.2-133.2
• /
• 2010

현재 융융탄산염 연료전지의 공기극으로 다공성의 lithiated NiO를 사용하고 있는데 이 재료의 경우 크게 두 가지의 문제점을 안고 있다. 첫 번째는 Ni이 전해질 내로 용해하는 것이고, 두 번째는 낮은 활성으로 인한 높은 공기극의 분극이다. Ni이 전해질로 용해되는 문제는 Co나 Fe를 코팅하여 공기극 표면에 $Li_x(Ni_yCo_{1-y})1-xO_2$나 $Li_x(Ni_yFe_{1-y})_{1-x}O_2$를 형성시켜 NiO의 전해질 내로 용해되는 것을 억제하는 방법이나 ZnO, MgO, $La_2O_3$ 등의 산화물을 NiO 표면에 코팅하여 전해질과 접촉을 막는 방식으로 해결하는 등 많은 연구가 이루어져 왔다. 하지만 연료극의 비해 상당히 높은 공기극의 분극으로 인해 큰 전압손실이 일어나 용융탄산염 연료전지 성능이 낮아지는 문제의 경우 이를 해결하고자 하는 연구는 상대적으로 많이 진행되지 못한 상태이다. 특히 현재 용융탄산염 연료전지의 장기수명화를 위해 기존의 작동온도인 $650^{\circ}C$ 보다 다소 낮은 온도인 $600{\sim}620^{\circ}C$에서 작동하려는 움직임이 있다. 작동 온도가 내려가면 전해질이 휘발되는 속도가 낮아져 전해질 부족에 따른 운전시간이 줄어드는 문제를 해결할 수 있어 장기 수명화를 위해서는 작동온도를 낮추는 것이 매우 유리하다. 하지만 작동 온도가 내려가면서 양 전극에서 일어나는 전기화학 반응 속도가 느려지기 때문에 각 전극에서의 활성화 분극으로 인한 전압손실은 더욱 커질 수밖에 없다. 특히 연료극의 수소산화반응 속도는 공기극의 산소환원반응에 비해 매우 빠르기 때문에 작동 온도가 내려감에 따라 연료극의 분극이 커지는 것에 비해 공기극의 분극이 급격히 커지게 된다. 따라서 운전온도가 낮아지는 상황에서는 낮은 작동온도에서도 성능감소가 적게 일어나 0.8V 이상 운전(150mA/$cm^2$, 단위전지 기준)이 가능한 공기극의 개발이 매우 필요한 실정이다. 이를 해결하고자 본 연구에서는 고체 산화물 연료전지의 공기극의 재료로 많이 연구되고 있는 혼합전도성 물질의 페로브스카이트 구조의 물질을 기존 NiO 전극에 코팅하여 새로운 공기극을 개발하였다. 페로브스카이트 구조의 물질로 대표적인 LSCF 물질을 사용하였으며 LSCF를 코팅한 공기극을 이용한 단위전지에서 150mA/$cm^2$의 전류를 흘려주었을 때 0.84V의 성능을 1000hr 유지하였다. 이는 기존의 NiO 전극을 사용했을 때보다 15~20mV 높은 값이다. 낮은 작동온도에서도 좋은 성능을 보였는데, 기존의 NiO 전극의 경우 $630^{\circ}C$에서 0.79V의 성능을 보인 반면 LSCF가 코팅된 공기극의 경우 $620^{\circ}C$에서 0.811V의 매우 좋은 성능을 보였다. 이는 LSCF의 산소이온전도성 및 전기전도성이 공기극에서의 분극을 낮추어 성능을 증가시키는 것으로 보인다.

• Journal of the Korean Chemical Society
• /
• v.47 no.5
• /
• pp.472-478
• /
• 2003

In this work, the zeolite/diglycidylether of bisphenol A(DGEBA) systems were investigated in terms of the cure kinetics and mechanical interfacial properties of the composites. The 4, 4-diamino diphenyl methane(DDM) was used as a curing agent for epoxy. Two types of zeolite(PZ) were prepared with 15 and 35 wt% KOH treatments(15-BZ and 35-BZ, respectively) for 24 h, and their surface characteristics were studied by X-ray photoelectron spectroscopy (XPS) and X-ray diffraction(XRD). Cure kinetics of the composites were examined in the context of differential scanning calorimetry(DSC), and mechanical interfacial properties were investigated in critical stress intensity factor($K_{IC}$) and critical strain energy release rate($G_{IC}$). In the results of XPS and XRD, sodium ion(Na) of zeolite was exchanged for potassium ion(K), resulting from the treatment of KOH. Also, $Si_{2p}/Al{2p}$ composition ratios of the treated zeolite were increased, which could be attributed to the weakening of Al-O bond in framework. Cure activation energy($E_a$) of 15-BZ composites was decreased, whereas KIC and $G_{IC}$ were increased, compared with those of the pure zeolite/DGEBA composites. It was probably accounted that the acidity of zeolite was increased by surface treatments and the cure reaction between zeolite and epoxy was influenced on the increased acidity of zeolite.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.12 no.4
• /
• pp.202-209
• /
• 2002

The stochiometric mix of evaporating materials for the $CuInSe_2$single crystal thin films was prepared from horizontal furnace. To obtain the single crystal thin films, $CuInSe_2$compound crystal was deposited on thoroughly etched semi-insulating GaAs(100) substrate by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were $620^{\circ}C$ and $410^{\circ}C$, respectively. The crystalline structure of single crystal thin films was investigated by the photoluminescence and double crystal X-ray diffraction (DCXD). The carrier density and mobility of $CuInSe_2$single crystal thin films measured from Hall effect by van der Pauw method. From the photocurrent spectrum by illumination of perpendicular light on the c-axis of the $CuInSe_2$single crystal thin film, we have found that the values of spin orbit splitting $\Delta$So and the crystal field splitting $\Delta$Cr. From the photoluminescence measurement on $CuInSe_2$single crystal thin film, we observed free exciton ($E_x$) existing only high quality crystal and neutral bound exciton ($A^{\circ}$, X) having very strong peak intensity. Then, the full-width-at-half-maximum (FWHM) and binding energy of neutral donor bound exciton were 7 meV and 5.9 meV, respectivity. By haynes rule, an activation energy of impurity was 59 meV.

• Clean Technology
• /
• v.26 no.1
• /
• pp.30-38
• /
• 2020

The kinetic and thermodynamic parameters of Acid Red 66, adsorbed by granular activated carbon, were investigated on areas of initial concentration, contact time, and temperature. The adsorption equilibrium data were applied to Langmuir, Freundlich, Temkin, Redlich-Peterson, and Temkin isotherms. The agreement was found to be the highest in the Freundlich model. From the determined Freundlich separation factor (1/n = 0.125 ~ 0.232), the adsorption of Acid Red 66 by granular activated carbon could be employed as an effective treatment method. Temkin's constant related to adsorption heat (B_T = 2.147 ~ 2.562 J mol^-1) showed that this process was physical adsorption. From kinetic experiments, the adsorption process followed the pseudo-second order model with good agreement. The results of the intraparticle diffusion equation showed that the inclination of the second straight line representing the intraparticle diffusion was smaller than that of the first straight line representing the boundary layer diffusion. Therefore, it was confirmed that intraparticle diffusion was the rate-controlling step. From thermodynamic experiments, the activation energy was determined as 35.23 kJ mol^-1, indicating that the adsorption of Acid Red 66 was physical adsorption. The negative Gibbs free energy change (ΔG = -0.548 ~ -7.802 kJ mol^-1) and the positive enthalpy change (ΔH = +109.112 kJ mol^-1) indicated the spontaneous and endothermic nature of the adsorption process, respectively. The isosteric heat of adsorption increased with the increase of surface loading, indicating lateral interactions between the adsorbed dye molecules.

• Applied Chemistry for Engineering
• /
• v.2 no.2
• /
• pp.108-118
• /
• 1991

Interactions between pyridine hydrodenitrogenation (HDN) and m-cresol hydrodeoxygenation(HDO), and the kinetic analysis were studied over sulfided $CoMo/{\gamma}-Al_2O_3$ catalyst at the range of temperatures between 473 K and 723 K, the total pressures between $10{\times}10^5Pa$ and $50{\times}10^5Pa$, and the contact times between 0.0125 g-cat. hr/ml-feed and 0.03g-cat. hr/ml-feed. HDN of pyridine and HDO of m-cresol were inhibited by each other and the inhibition effect of HDO by pyridine is higher than that of HDN by m-cresol. But reactivity of m-cresol is higher than that of pyridine. The rate equations of pyridine and m-cresol were given to be ${\gamma}_{HDN}=k_{HDN}{\cdot}K_pC_p/(1+K_cC_c+K_pC_p)$ and ${\gamma}_{HDO}=k_{HDO}{\cdot}K_cC_c/(1+K_cC_c+K_pC_p)$ in terms of Langmuir-Hinshellwood-Hougen-Watson model. At each temperature, reaction rate constants and adsorption equilibrium constants were determined and activation energies of pyridine HDN and m-cresol HDO are 13.83kcal/mol, respectively and the heat of adsorption are -6.458 and -5.045kcal/mol, respectively.

• Food Science and Preservation
• /
• v.9 no.4
• /
• pp.429-433
• /
• 2002

The D-xylulokinase from Candida sp. L-16 was purified through a sequence of ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-100 and Sephadex G-200 gel filtration. The specific activity of the purified Dxylulokinase was increased to 23.2 fold and the yield was 11.2%. The enzyme was showed to be a single protein band by SDS-PAGE. The molecular weight of the enzyme was 150,000 dalton, this enzyme was identified to be a dimer with two subunits. The optimum conditions of the enzyme were pH 8.0 and 40$\^{C}$, respectively. The enzyme was relatively stable between pH 7.0 to pH 9.0, but it was unstable over 30$\^{C}$. The enzyme showed substrate specificity on D-xylulose, D-arabinose and D-ribose, Km value and Vmax for D-xylulose were 0.042 mM and 117 units/ml, respectively. The activation energy of the enzyme was 4.75 Kcal/mol. The one was inhibited by metabolic intermediates such as 6-phosphogluconic acid, 2-keto-gluconic acid. The enzyme was activated by EDTA and thiol compounds such as cysteine-HCI, DTT and glutathione.

• Clean Technology
• /
• v.27 no.1
• /
• pp.47-54
• /
• 2021

The adsorption of Acid Fuchsin (AF) on granular activated carbon (GAC) was investigated for isothermal adsorption and kinetics and thermodynamic parameters by experimenting with the initial concentration, contact time, temperature, and pH of the dye as adsorption parameters. In the pH effect experiment, the adsorption of AF on activated carbon showed a bathtub type with increased adsorption at pH 3 and 11. The adsorption equilibrium data of AF fit well with the Freundlich isotherm model, and the calculated separation factor (1/n) value was found in which activated carbon can effectively remove AF. The pseudo-second-order kinetic model fits well within 7.88% of the error percent in the adsorption process. According to Weber and Morris's model plot, it was divided into two straight lines. The intraparticle diffusion rate was slow because the stage 2 (intraparticle diffusion) slope was smaller than that of stage 1 (boundary layer diffusion). Therefore, it was confirmed that the intraparticle diffusion was a rate-controlling step. The activation energy of AF (13.00 kJ mol-1) corresponded to the physical adsorption process (5 - 40 kJ mol-1). The free energy change of the AF adsorption by activated carbon showed negative values at 298-318 K. As the spontaneity increased with increasing temperature. The adsorption of AF was an endothermic reaction (ΔH = 22.65 kJ mol-1).

• Journal of the Korean Chemical Society
• /
• v.38 no.12
• /
• pp.873-879
• /
• 1994

A series of samples of solid solutions in the $Sr_xEu_{1-x}FeO_{3-y}(0.00{\leq}x{\leq}1.00)$ system has been prepared at $1200^{\circ}C$ under an atmospheric air pressure. The structures of solid solutions are studied by X-ray diffraction, thermal, Mohr salt, and Mossbauer spectroscopic analyses. Their physical properties are also discussed with the electrical conductivities. X-ray diffraction data for the compositions of x = 0.00, 0.25, and 1.00 are assigned to the orthorhombic and the compositions of x = 0.50 and 0.75 to the cubic systems. The lattice volume reduced to cubic cell increases with the x value. The mole ratio of $Fe^{4+}$ iometric chemical formulas of the system are formulated from the x, $\tau$, and y values. The mixed valency state of Fe ions, the oxygen coordination, and covalent bond character are discussed with the Mossbauer spectroscopic data. The activation enegy of the electrical conductivities depends on the $\tau$ value in the temperature range of -$100^{\circ}C$ to $600\circC$ under the air pressure. The Mossbauer spectrum and electrical conductivity of the solid solutions are discussed with nonstoichiometric chemical compositions.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.11 no.3
• /
• pp.21-27
• /
• 1982

A kinetical approach for the non-enzymatic browning reaction of the dried file-fish was studied. The reaction rates revealed a tend to increase with increasing water activity and showed the maximum at $0.75\;a_w$ The activation energies obtained from the Arrhenius plot ranged 12.5 to 16.5 Kcal/mole. From these energies of activation, the $Q_{10}$ values at $45^{\circ}C$ showed 1.9 to 2.3 and both activation energy and $Q_{10}$ values were reduced with increase in $a_w$ Shelf-lives, the time to reach an 0.15 O.D./g solid at which severe brown color change could be de ectable, decreased rapidly as the temperature and water activity increase. A storage study under a square-wave fluctuating temperature condition (at 35 and $55^{\circ}C$ periodically with 7 days interval), the rate constants at all water activities used in the experiments were higher than those at $45^{\circ}C$, the mean temperature of the cycle which affects other kinetic parameters including activation energies, $Q_{10}$ values and finally the shelf-lives. The data obtained from the fluctuating temperaure storage study will be used in prediction of shelf-life. The shelf lives assessed at $25^{\circ}C$ from the accelerated shelf-life tests ranged from 179 daysat $0.75\;a_w$ to 302 days at $0.44\;a_w$.