• Applied Biological Chemistry
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• v.1
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• pp.12-20
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• 1960

고위생산답(高位生産畓) 22개(個)와 매년(每年) 호마엽고병(胡麻葉枯病)을 발생(發生)시키는 저위생산답(低位生産畓) 18개토양(個土壤)을 분석(分析)하고 활성철(活性鐵)과 열염산(熱鹽酸)에 녹는 철(鐵)의 분포상태(分布狀態)를 조사(調査)하였으며 그 결과(結果)는 아래와 같다. 1. 작토중(作土中)의 활성철(活性鐵)의 함량(含量)과 청취(聽取)한 정조수량간(正粗收量間)에는 밀접(密接)한 정상관(正相關) (${\gamma}=0.68$, 고등(高等)의 유의성(有意性)이 있음)이 있다. 2. 고위생산답(高位生産畓) 토양(土壤)의 활성철(活性鐵) 및 열염산가용철(熱鹽酸可溶鐵)은 저위생산답(低位生産畓) 토양(土壤)에서의 그것보다 현저(顯著)히 많았으며 각(各) 토양별(土壤別) I 층(層)의 그 평균함량(平均含量)과 열염산가용철(熱鹽酸可溶鐵)에 대(對)한 활성철(活性鐵)의 비율(比率)은 아래와 같다. 고위생산답(高位生産畓) 조사점수(調査點數) 활성철(活性鐵)% 활성철(活性鐵)/염산가용철(鹽酸可溶鐵) 잔적토(殘積土) 6 1.313 0.374 하성토(河成土) 9 1.334 0.335 해성토(海成土) 5 1.120 0.382 평균(平均) 20 1.224 0.359 저위생산답(低位生産畓) 조사점수(調査點數) 활성철(活性鐵)% 활성철(活性鐵)/염산가용철(鹽酸可溶鐵) 잔적토(殘積土) 5 1.15 0.370 하성토(河成土) 8 0.472 0.191 해성토(海成土) 5 1.068 0.362 평균(平均) 18 0.808 0.288 그러나 표(表)에서와 같이 잔적토(殘積土) 저위생산답(低位生産畓) 각층(各層)의 철(鐵)은 고위생산답(高位生産畓)에서 보다 낮지 않았다. 3. 해수(海水)의 영향(影響)을 받지 않은 고위생산답(高位生産畓)에서는 표층토(表層土)의 세탈(洗脫)이 적었으나 동(同) 저위생산답(低位生産畓) 및 해성토(海成土)에서는 그 세탈(洗脫)이 크고 동세탈물(同洗脫物)은 심층(心層)에 집적(集積)되여 있다. 4. 해성토(海成土)에서는 고위생산답(高位生産畓)이나 저위생산답(低位生産畓)을 막론(莫論)하고 집적층직하(集積層直下)에 활성철량(活性鐵量)이 심(甚)히 적은 층(層)이 있다. 그리고 집적층(集積層)은 고위생산답(高位生産畓)에서는 II-III층(層)(지표면(地表面)으로부터 60cm이내(以內))에 저위생산답(低位生産畓)에서는 I-II층(層)(지표면(地表面)으로부터 대개(大槪) 30cm이내(以內))에 위치(位置)한다. 5. 같은 고위생산답(高位生産畓) 및 저위생산답(低位生産畓)에서는 토성간(土性間)의 활성철함량(活性鐵含量)에 큰 차이(差異)가 없다. 6. 내산씨(內山氏)의 4개(個)기본(基本)토양형별(土壤型別)로는 동일(同一)토양형((土壤型)이라도 고위(高位) 저위생산답별(低位生産畓別)로 활성철(活性鐵)의 함량(含量)에는 차이(差異)가 크다.

• Korean Journal of Microbiology
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• v.31 no.1
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• pp.93-98
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• 1993

To investigate the organic matter transformation in aquatic environment, seasonal fluctuations of heterotrophic activity and microbia] extracellular enzyme activity were studied in Paldang Lake, Korea. The turnover time in the water column and the sediment at the station I fluctuated between 3 -I ,300 hrs and 17-170 hrs for glucose, 5 -1.900 hrs and 15-240 hrs for protein hydrolysate and 4-350 hrs and 15-230 hrs for acetic acid, respectively, indicating that the seasonal turnover time of organic substrates fluctuated drastically. The respiration ratios of glucose. protein hydrolysate and acetate were 23-32%, 38-41% and 22-28% in the water column and 34%, 61% and 41% in the sediment. respectively. These results showed that the respiration ratios in the sediment were higher than those in the water column regardless of kinds of organic substrates. The bacterial extracellular enzyme activities of $\alpha$-glucosidase. $\beta$-glucosidase, N-acetyl-$\beta$-D-glucosaminidase and aminopeptidase were 32-44%. 31-32%, 18-34% and 61-67% in the water column, and 34%. 40%, 23% and 65% in the sediment. respectively.

• Tuberculosis and Respiratory Diseases
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• v.53 no.4
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• pp.409-419
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• 2002

Background : The therapeutic effects of surfactant on acute lung injury derive not only from its recruiting action on collapsed alveoli but also from its anti-inflammatory effects. Pro-apoptotic action on alveolar neutrophils represents one of the important anti-inflammatory mechanisms of surfactant. In the present study, we evaluated the effects of sufactant on the apoptosis of human peripheral and rat alveolar neutrophils. Methods : In the (Ed- the article is not definitely needed but it helps to separate the two prepositions 'in') in vitro study, human neutrophils were collected from healthy volunteers. An equal number of neutrophils ($1{\times}10^6$) (Ed-confirm) was treated with LPS (10, 100, 1000ng/ml), surfactant (10, 100, $1000{\mu}g/ml$), or a combination of LPS (1000ng/ml) and surfactant (10, 100, $1000{\mu}g/ml$). After incubation for 24 hours, the apoptosis of neutrophils was evaluated by Annexin V method. In the in vivo study, induction of acute lung injury in SD rats by intra-tracheal instillation of LPS (5mg/kg) was followed by intra-tracheal administration of either surfactant (30mg/kg) or normal saline (5ml/kg). Tenty-four hours after LPS instillation, alveolar neutrophils were collected and the apoptotic rate was evaluated by Annexin V method. In addition, changes of the respiratory mechanics of rats (respiratory rate, tidal volume, and airway resistance) were evaluated with one chamber body plethysmography before, and 23 hours after, LPS instillation. Results : in the in vitro study, LPS treatment decreased the apoptosis of human peripheral blood neutrophils (control: $47.4{\pm}5.0%$, LPS 10ng/ml; $30.6{\pm}10.8%$, LPS 100ng/ml; $27.5{\pm}9.5%$, LPS 1000ng/ml; $24.4{\pm}7.7%$). The combination of low to moderate doses of surfactant with LPS promoted apoptosis (LPS 1000ng/ml + Surf $10{\mu}g/ml$; $36.6{\pm}11.3%$, LPS 1000ng/ml +Surf $100{\mu}g/ml$; $41.3{\pm}11.2%$). The high dose of surfactant ($1000{\mu}g/ml$) decreased apoptosis ($24.4{\pm}7.7%$) and augmented the anti-apoptotic effect of LPS (LPS 1000ng/ml + Surf $1000P{\mu}g/ml$; $19.8{\pm}5.4%$). In the in vivo study, the apoptotic rate of alveolar neutrophils of surfactant-treated rats was higher than that of normal saline-treated rats ($6.03{\pm}3.36%$ vs. $2.95{\pm}0.58%$). The airway resistance (represented by Penh) of surfactant-treated rats was lower than that of normal saline-treated rats at 23 hours after LPS injury ($2.64{\pm}0.69$ vs. $4.51{\pm}2.24$, p<0.05). Conclusion : Surfactant promotes the apoptosis of human peripheral blood and rat alveolar neutrophils. Pro-apoptotic action on neutrophils represents one of the important anti-inflammatory mechanisms of surfactant.

• Journal of Soil and Groundwater Environment
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• v.11 no.6
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• pp.43-52
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• 2006

Effectiveness of activated soil containing directly enriched atrazine-degrading soil microorganisms as an inoculant to bioaugment degradation of atrazine in soils was investigated. A Wooster silt loam (Typic Fragiudalf) was spiked with atrazine at a rate of 4 mg/kg soil three successive times to create activated soil. Atrazine degradation was significantly enhanced (p < 0.05) after the first treatment. After the second treatment, there was an increase in the number, based on MPN, of microorganisms utilizing atrazine as a C- and N-source by 3 logs and 1 log of magnitude, respectively. Inoculation of typical agricultural soils collected from Ohio with activated soil at a rate as low as 0.5% reduced the extractable atrazine remaining in soils to the level below 2% of that initially recovered (initially added at a rate of 4 mg/kg soil) after 4 days. Inoculation at a higher rate was required to achieve the same result in soils with non-typical properties (pH of 4.5 or organic matter of 43% w/w). Activated soil was stable, in terms of atrazine degradation activity, at least up to 6 months when it was kept at low temperature (< $10^{\circ}C$) and moistened (water content above 15%). The results of this study indicate that microorganisms capable of degrading atrazine are relatively easily enriched in soil to create activated soil. Use of activated soil can be a practical option for bioremediation of contaminated soils.

• Journal of the Korea Organic Resources Recycling Association
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• v.4 no.2
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• pp.55-62
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• 1996

The purpose of this study is the evaluation of adsorption capacity of casts for heavy metals comparing with the activated carbon. The casts was obtained from vermicomposting of the mixed organic sludges which were generated from the treatment facilities for leather wastewater and cattle wastewater. The physico-chemical characteristics of cast was investigated. Also, the batch adsorption experiments of cast and activated carbon for heavy metals were carried out, and the results were analyzed by Freundlich isotherm. The buffering capacity to the acidic wastewater was founded in the cast, and the cation exchange capacity of cast impling adsorption capacity for soluble substances was evaluated as about 55me/100g. Those were implied that the cast have a large potential as a good adsorbent for soluble pollutants in wastewater. From the results of batch experiments, the removal efficiencies of tested various heavy metals including Pb, Cu, Cd, and Cr were very high value as 89-98% for the activated car-bon, and 80~95% for the casts except for Zn. The adsorption equilibriums for the two materials were achieved within 90 minutes. The order of preferable metals in the adsorption was found to be Pb>Cu>Cd>Cr>Zn on the cast and to be Pb>Cd>Cu>Cr>Zn on the activated carbon, respectively. From the above results, it might be con-cluded that cast is effectively available as a good adsorbent to treating the heavy metal bearing wastewater.

• Journal of the Korean Geotechnical Society
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• v.27 no.3
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• pp.19-26
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• 2011

Compaction tests have been performed to investigate the compaction characteristics of sands and clays with organic mixture. Weathered granite soil, kaolinite, and granulated carbon were used as the alternatives of sand, clay, and organics, respectively. The soapy water which is a kind of surfactant solutions was also used as water substitute to see the engineering properties changes of each soil. As seen when water was used, the optimum moisture contents increased and the maximum dry unit weight decreased for the soil with surfactants as the percentage of the organic contents increased. Surfactants slightly improved the compaction efficiency at low compactive energy level for the weathered granite soil with organics. As the organic contents increased for clays with surfactants, the optimum moisture contents decreased and the maximum dry unit weight increased. Surfactants slightly improved the compaction efficiency of clays with organics at all levels of compaction energy.

• KSBB Journal
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• v.24 no.1
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• pp.53-58
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• 2009

This study was focused on the investigation of the characteristics of TPH and BTEX removal in oil-contaminated sandy soil and fine soil with injection of microorganisms, nutrients, and surfactants. As the result of the experiments maintained moisture contents by 10${\sim}$20%, the TPH removal efficiency in oil-contaminated sandy soil was the highest in C-1 (microorganisms+nutrients), and the efficiency in C-2 (microorganisms+nutrients+surfactants) was higher than the efficiency in C-0(microorganisms). In 81 days, TPH removal efficiency in case of C-0, C-1 and C-2 showed 51%, 83%, 63% respectively. The results of D group with fine soil showed similar trends as C group, but the TPH removal efficiency of D group was lower than that of C group. Those of both C and D group were the highest in 1 group (microganisms+nutrients). The pH of fine soil was some lower than that of sandy soil or was similar to sandy soil. In 14 days, BTEX removal efficiency in case of C-0, C-1, C-2, D-0, D-1 and D-2 showed 99.8%, 99.4%, 96.0%, 99.5%, 99.2%, 96.3% respectively. Those of both C and D group were the highest in 0 group (microganisms).

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.158.1-158
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• 1995

• Applied Biological Chemistry
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• v.47 no.1
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• pp.149-153
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• 2004

전통된장 제조방법으로서 원료 혼합비, 메주의 건조시간 및 온도, 메주와 된장의 발효 시간 및 온도, 된장 간장의 분리 여부, 된장 숙성(aging) 시간을 조사하였다. 혈액응고 저해 활성(anticoagulant activity)을 fibrin clotting assay법으로 분석하여 혈액응고 저해활성과 제법간의 상관 관계를 검토하였다. 숙성 기간이 길수록 혈액응고 저해활성이 높게 나타나는 경향을 보였으나 선형적인 상관관계보다는 비선형적으로 양의 상관관계를 갖는 것으로 판단되었다. 이상의 결과를 토대로 혈액응고 저해활성이 높은 2종의 된장시료를 선발하였으며 숙성기간이 180일 이상일 경우 전통된장의 혈액응고 저해활성이 높은 것으로 판단되었다.

• Proceedings of the KAIS Fall Conference
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• 2009.05a
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• pp.874-877
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• 2009

본 연구에서는 수용액 내의 오염물질 분해를 위하여 개발한 광촉매가 코팅된 실리카 비드의 광분 해반응 사용에 따른 활성저하 문제를 해결하기 위하여 반응에 사용한 비드의 활성을 향상시킬 수 있는 재생 방법에 관한 실험을 수행하였다. 비드의 재생방법으로 표면 세정법을 선택하였으며, 세정액으로는 물(증류수), 계면활성제, 아세톤 등 세정력이 서로 다른 3종의 용액을 사용하였다. 재생 과정은 서로 다른 3종의 세정액으로 반응에 사용하여 활성이 떨어진 비드를 세정한 후, 소성온도를 $100^{\circ}C$, $200^{\circ}C$, $300^{\circ}C$로 달리하여 30분간 처리하였다. 재생 처리과정은 각 1~3회 반복 수행하였으며, 서로 다른 조건에서 재생된 비드의 활성은 수용액 내의 methylene blue 광분해율로 측정하였다. 연구결과, 재생한 비드의 활성은 아세톤으로 세정한 후, $100^{\circ}C$에서 30분간 소성하였을 때 가장 우수한 것으로 나타났으며, 이러한 기초 연구결과를 토대로 현재보다 효율적인 재생 기술에 관한 연구를 수행 중에 있다.