• Title/Summary/Keyword: 활성온도

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Effects of Temperature on the Changes of Enzymatic Activities and Metabolite during Wheat nuruk Fermentation (밀누룩 발효기간 동안 효소와 대사체 변화에 대한 온도의 영향)

  • Lee, Se Hee;Baek, Seong Yeol;Kang, Ji-Eun;Jeon, Che Ok;Kim, Dae-Hyuk;Kim, Myoung-Dong;Yeo, Soo-Hwan
    • Microbiology and Biotechnology Letters
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    • v.43 no.4
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    • pp.378-384
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    • 2015
  • Nuruk is a fermentation agent, which has been used for the production of traditional Korean alcoholic beverages. The objective of this study was to investigate the effects of temperature on nuruk fermentation. One wheat nuruk sample was fermented at $36^{\circ}C$ for 30 days (TN-A) and another at $45^{\circ}C$ for 10 days followed by $36^{\circ}C$ for 20 days (TN-B). The activities of ${\alpha}$-amylase, glucoamylase, and acidic protease, as well as metabolite contents were measured. Initially, the enzymatic activities increased rapidly regardless of the fermentation temperature. After 3 days of fermentation, the enzymatic activities were maintained in TN-A, but gradually decreased in TN-B until the end of fermentation process. Metabolite analysis using $^1H$-NMR showed that the levels of glucose, glycerol, fructose, mannitol, and lactose initially increased quickly and then decreased in TN-A. However, they initially decreased and then were maintained over the fermentation period in TN-B. The contents of glycine, proline, and serine were higher in TN-A than in TN-B. This study suggests that a constant temperature of approximately $36^{\circ}C$ is appropriate for achieving high amylolytic and proteolytic activities in the production of wheat nuruk.

Optimal Surfactant Structures for Cosurfactant-Free Microemulsion Systems (III) -Nonylphenol and Tridecyl Alcohol Hydrophobes- (계면활성제의 구조가 Microemulsion 형성에 미치는 영향(제 III보) -친유기 Nonylphenol과 Tridecyl alcohol-)

  • 김천희
    • Journal of the Korean Society of Clothing and Textiles
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    • v.18 no.4
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    • pp.473-479
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    • 1994
  • 보소계면활성제 없이 microemulsion을 형성하는 최적의 계면활성제 구조를 찾기 위한 일련의 연구 중 세번째로 propoxylated nonylphenol sulfates와 ethoxylated 혹은 propoxylated highly-branched tridecyl alcohol sulfates를 연구하였다. 온도, NaCl농도, 보조계면활성제 농도, ethylene oxide몰수(EON), propylene oxide몰수(PON) 등을 변인으로 하여 phase behavior 실험을 한 결과 tridecyl alcohol 친유기를 갖는 계면활성제가 보조계면활성제 없이 microemulsion을 형성하였다. $C_{13}O(EO)_mPO)_nSO_3Na$ 일반식으로부터 m과 n을 변경시킴으로써 계의 다양한 조건을 만족시키는 계면활성제를 디자인할 수 있었다.

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Production of Fructooligosaccharides by an Amyloglucosidase (Amyloglucosidase에 의한 Fructooligosaccharides의 생산)

  • 윤종원;이민규송승구
    • KSBB Journal
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    • v.9 no.2
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    • pp.127-132
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    • 1994
  • A new method of fructooligosaccharides production was investigated by an amyloglucosidase using sucrose as a substrate. Optimum reaction conditions were as follows: sucrose concentration, 700g/$\ell$; pH 5.5; temperature, $55^{\circ}C$; enzyme dosage, 48 units per gram sucrose. At the optimized reaction conditions, 41.5% of fructooligosaccharides were produced after 25 hours. A hydrolyzing activity was stronger than transfructosylting activity at low sucrose concentrations, resulting in low production rate of fructooligosaccharides. The optimum pH and temperature in both transfructosylating(pH 5.5, $60^{\circ}C$) and hydrolyzing activity(pH$4.75^{\circ}C$)were significantly different from each other. The amyloglucosidase also utilized fructooligosaccharides as a substrate and glucose seemed to be an inhibitor.

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마이크로파 에너지를 이용한 톨루엔 흡착-냉각 응축에 관한 연구

  • Do, Sang-Hyeon;Kim, Seong-Uk;Kim, Yun-Gap;Choe, Seong-U
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2006.11a
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    • pp.124-126
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    • 2006
  • 마이크로파 에너지의 열원을 이용하여 흡착된 톨루엔을 탈착함으로써 활성탄의 재생에 관한 실험을 실시하였다. 마이크로파를 조사함에 따라 흡착제의 온도가 급격히 상승하여 톨루엔이 탈착할수 있는 온도까지 빠른 시간에 도달하였으며, 이 열에 의하여 톨루엔이 탈착함을 알수 있었다.

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Silica-encapsulated ZnSe Quantum Dots as a Temperature Sensor Media (온도센서용 실리카에 담지된 ZnSe 양자점 소재)

  • Lee, Ae Ri;Park, Sang Joon
    • Applied Chemistry for Engineering
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    • v.26 no.3
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    • pp.362-365
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    • 2015
  • Silica encapsulated ZnSe quantum dots (QDs) were prepared by employing two microemulsion systems: AOT/water/cyclohexane microemulsions containing ZnSe quantum dots with NP5/water/cyclohexane microemulsions containing tetraethylorthosilicate (TEOS). Using this method, cubic zinc blende nanoparticles (3 nm in diameter) were synthesized and encapsulated by silica nanoparticles (20 nm in diameter). The temperature dependence of photoluminescence (PL) for silica-encapsulated ZnSe QDs was investigated to evaluate this material as a temperature sensor media. The fluorescence emission intensity of silica-encapsulated ZnSe nanoparticles (NPs) was decreased with an increase of ambient temperature over the range from $30^{\circ}C$ to $60^{\circ}C$ and a linear relationship between the temperature and the emission intensity was observed. In addition, the temperature dependence of PL intensity for silica-encapsulated ZnSe NPs showed a reversible pattern on ambient temperature. A reversible temperature dependence of the luminescence combined with its insensitivity toward quenching by oxygen due to silica coating established this material as an attractive media for temperature sensor applications.

Pectin Transeminase produced by Aspergillus niger and two yeast species. (Aspergillus niger와 이종의 Yeast에 의한 Pectin Transeliminase의 생성 및 그 특성)

  • 민경희;이영자;김치경
    • Microbiology and Biotechnology Letters
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    • v.8 no.4
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    • pp.229-235
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    • 1980
  • Pectin transeliminase (PTE) was produced by Saccharomyces cerevisiae, Schizosaccharomyces pombe 4683, and Aspergillus niger in the media containing 2% pectin and examined for its characteristics. The Production of the enzyme was higher by Asp. niger than by the two yeast strains, showing that the PTE activity was proportional to reducing power. The enzyme was proved to reduce pectin and produce 4, 5- unsaturated galacturonic acid. The optimum activity of the PTE was found to be at pH 6.0 and $50^{\circ}C$. The activities of these enzyme were stable below $50^{\circ}C$ but decreased at the higher temperature. Substrate inhibition of the PTE activities was appeared at high concentrations of pectin. Those PTE activities were increased under 0.6M of KCI and NaCI, but that maximal activities at the concentration of 0.2M MgC $l_2$.

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Adsorption Characteristics of Functionalized Activated Carbon for High Temperature CO2 Capture (고온 이산화탄소 포집을 위한 기능성 활성탄의 흡착특성)

  • Choi, Sung-Woo;Lee, Cheol-Gyu
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.3
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    • pp.175-181
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    • 2015
  • Activated carbon impregnated with polyethyleneimine (PEI) was evaluated as a functionalized adsorbent for $CO_2$ capture. The $CO_2$ adsorption characteristics of the adsorbents was undertaken using GC/TCD, BET surface area and FT-IR. A series of adsorbents were synthesized by impregnating 10, 30, 50 wt% of PEI on activated carbons and were investigated $CO_2$ adsorption capacity at high and low adsorption temperature. The $CO_2$ adsorption capacity at $20^{\circ}C$ and $100^{\circ}C$ was as follow: AC > PEI(10)-AC > PEI(30)-AC > PEI(50)-AC at $20^{\circ}C$ and PEI(10)-AC > PEI(30)-AC > PEI(50)-AC > AC at $100^{\circ}C$. Adsorption capacities of amine functionalized AC was lager than virgin AC at high temperature due to chemisorption by amino-group content. From the results, the PEI(10)-AC showed one of the most promising adsorbents for $CO_2$ capture from flue gas at high temperature.

Characteristics of the Cell Wall Lytic Enzyme of Anabaena cylindrica from Penicillium oxalicum(HCLF-34) (Penicillium oxalicum(HCLF-34)으로부터 분비되는 Anabaena cylindrica 세포벽 분해효소의 특성)

  • 현성희;최영길
    • Korean Journal of Microbiology
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    • v.35 no.3
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    • pp.231-236
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    • 1999
  • The fuugus(Penicil1ium oralicum; HCLF-34) secreted the cyanobacteria lytic enzyme which had a molecular weight of about 22 kDa, a optimum temperature of $20^{\circ}C$, a optimum pH of 3.5, and a temperature-stable up to $50^{\circ}C$. The chemical ions such as sodium, potassium, barium, magnesium. and mangan ions appeared positive activity. but calcium, iron, copper ions, EDTA, and PMSF displayed negative activity: this results were the same as the characterilics of other cell wall lytic enzymes. This extracellular enzyme showed lytic aclivily against SDS-insoluble peptidoglycan of Anabaenrr cylinrlrica. The cell wall lylic enzyme of Penicilliurn oxalicum(HCLF-34) seemed to be glycosidase-like enzyme in the fact that ihe concentration of rcducing sugar was increased when the peptidoglycan of Anabaena qlinrlricn md Micrococcus luteus reacted with this enzyme

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Isolation and Characterization of Bacillus sp. WRD-2 Extracellular Protease from Soil (토양에서 분리한 Bacillus sp. WRD-2가 생산하는 Extracellular Protease의 특성)

  • Ok, Min;Seo, Won-Seok;Cha, Jae-Young;Cho, Young-Su
    • Applied Biological Chemistry
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    • v.44 no.4
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    • pp.246-250
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    • 2001
  • In order to produce alkaline protease, psychrotrophic Bacterium which have high enzyme activity, was isolated by using enrichment culture from soil samples and identified as genus Bacillus sp. The optimal pH and temperature for the enzyme activity were pH 6 and $40^{\circ}C$. The temperature range of high enzyme activity was $20{\sim}40^{\circ}C$. The optimal initial pH of culture condition for enzyme was pH 6. The most favorable carbon and nitrogen sources for the production of protease by Bacillus sp. WRD-2 were 3% maltose and 4% yeast extract, respectively.

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Sorption Behavior of Acetic Acid onto Activated Carbons (활성탄에서의 아세트산 흡탈착 거동)

  • Lee, Chae-Young;Chung, Jin-Suk;Shin, Eun-Woo
    • Korean Chemical Engineering Research
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    • v.46 no.6
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    • pp.1130-1134
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    • 2008
  • Acetic acid has been used as a solvent in the process of manufacturing terephthalic acid. Although the used acetic acid has been mainly separated and recovered through the distillation process, adsorption process can be applied to recover a small amount of acetic acid remaining in the stream after the distillation process. In this study, activated carbon was selected as an adsorbent for acetic acid and the effects of temperature and acid treatment on adsorption capacity were investigated. The adsorption capacities of activated carbon for acetic acid were 0.176 mmol/g at 303 K and 0.118 mmol/g at 343 K, respectively. Adsorption capacity decreased with increasing temperature. The acid treatment of the activated carbon induced the increase in adsorption capacity, which was ascribed to increase in surface functional groups such as phenolic hydroxyl groups and carboxilic acid groups on the carbon surface. In the results of acetic acid desorption, 89% of adsorbed acetic acid was desorbed from activated carbon.