• KSBB Journal
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• v.7 no.1
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• pp.21-26
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• 1992

In order to examine the presence of brassinosteroid substance in sea mustard(Undaria pinnatifida), leaves of sea mustard were extracted with MeOH. The extract was purified by slovent fractionation, counter current distribution, silica gel adsorption chromatography, charcoal adsorption chromatography, Bondesil chromatography, and reverse phase HPLC, successively. The activity was monitored by the rice inclination test and its prescence could be confirmed in each purification step. Although sea mustard contained a less amount of the active substance than the vegetative tissue of higher plants, brassinosteroid was clearly present endogenously in sea mustard. We acknowledge that our work is probably the first publication reporting the presence of brassinosteroid in marine algae plants.

• Journal of the Korean Wood Science and Technology
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• v.21 no.3
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• pp.82-86
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• 1993

은행나무 추출물의 생리활성에 대한 실험결과, 그 추출물이 GAG release에 의한 IL-1의 억제효과(87%)를 나타냄을 관찰하였다. 그 추출물을 chromatography로 분리한후 분광학적 분석을 이용하여 그 함유물질들이 anarchardic acid 및 그와 유사한 페놀성 물질임을 밝혔다. 각 성분에 대하여 활성효과를 다시 실험한 결과, 주성분인 IIIb와 IIIc는 활성효과를 보이지 않았으며, 단지, 소량물질인 Va와 Vb만이 중간 정도의 효과(각각 43과 55%)를 나타내었다. 이 결과에 의하면, 은행나무의 추출성분이 IL-1의 억제효과를 분명히 나타내나, 그 효과가 주성분에 기인하는 것이 아님을 보여 주었다.

• Geotechnical Engineering
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• v.13 no.2
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• pp.55-64
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• 1997

Laboratory tests were performed to desorb organic contaminants spiked onto soil particles by using surfactants. Nonionic and anionic surfactants were tested as potential desorbing solvents for extracting quinoline and 2-napthol adsorbed on soil particles. Ahionisc stirfactant remediated approximately 80% and 90% of quinoline and 2-napthol respectively and appeared to be more effective than nonionic surfactant in remediating those compounds, Comparison between simple deionized water washing of the organic contaminated soil and a given surfactant technique evaluated the improvement by the application of a Burfactant to the promotion of desorption.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.04a
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• pp.98.5-99
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• 1978

항생물질과 RNA 분해효소를 동시에 생산하는 방선균의 한 균주를 토양으로부터 분리하여 이 균주가 생산하는 RNA 분해효소의 물리화학적 성질 및 분해산물에 대해 검토하였다. 효소반응의 최적 pH 및 온도는 명명 pH 5.6과 $50^{\circ}C이었다.$ $37^{\circ}C$ 에서 90분간 열처리 시켰을 때, 이 효소의 활성은 비교적 안정하였지마는 $50^{\circ}C$ 에서 90분간 열처리 시켰을 때는 효소활성이 심하게 저하되었다. 이 효소의 활성은 $Ba^{2+}$ 에 의하여 50% 정도의 저해 작용을 나타내었지만 EDTA에 의해서는 저해되지 않았다. 이 효소에 의한 RNA분해로 이 효소가 대사산물로서 guanosine, adenosine과 밝혀지지 않은 두 가지의 핵산관연물질을 생산함을 알 수 있었다. 제2보에서는 ENase의 효소학적 성질을 검토하였으며 이후 항생물질 측면에서 검토할 예정이다.

• Proceedings of the KAIS Fall Conference
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• 2001.05a
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• pp.295-297
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• 2001

Cystocin, a derivative of Puromycin, is a new material derived from Streptomyces sp GCA0001, new strain of Actinomycetes spiecies. This compound has outstanding biological activities in anti-bacteria, anti-tumor and anti-virus than former Puromycin compounds. And it is chosen by natural selection processing through extraction, isolation and purification from , so it may replace old Puromycins in most applications.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1403-1408
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• 2000

The methanol soluble fraction of the hot water extracts from Hovenia dulcis Thunb showed antioxidative and antimicrobial activity. The methanol fraction was successively purified with solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography, and octadecylsilane column chromatography. The purified active substances were isolated by high performance liquid chromatography. The isolated substances were identified as 3-methoxy-4-hydroxybenzoic acid (vanillic acid) and 3-methoxy- 4-hydroxycinnamic acid (ferulic acid) by LC-MS and GC-MS. Vanillic acid and ferulic acid showed antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and yeast. The DPPH-radical scavenging activity of ferulic acid appeared more active than that of vanillic acid. DPPH-radical scavenging concentration of ferulic acid and vanillic acid were $14\;{\mu}g/mL\;(SC_{50})$, $100\;{\mu}g/mL\;(SC_{10})$, respectively.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2000.11a
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• pp.91-95
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• 2000

계면활성제를 이용하여 토양에 존재하는 유기 오염물질을 제거하는데 있어서, 계면활성제가 토양과의 상호작용시 투수계수를 상당히 ($10^2$ 이상) 감소시키면 이 복원방법은 실용성이 없다 그러므로 본 연구의 목적은 선택된 계면활성제와 투수계수 사이의 상관 관계를 고찰하는데 있다. 본 연구 결과 계면활성제(DOSL) 사용 후 증류수와의 상대적 비교치로써 투수계수는 21~35 % 감소하였다. 이 감소 수치의 의미는 선택된 계면활성제(DOSL)는 토양 복원 연구에 사용될 수 있음을 보여준다.

• The Journal of Internal Korean Medicine
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• v.21 no.2
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• pp.219-225
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• 2000

산화적인 스트레스가 여러가지 신경 및 비신경계에서의 병리원인으로 알려져 있다. 퇴행성 뇌질환에 대한 예방과 치료에는 항산화 방어기술이 주요대상이며 스테로이드 분자중에서 estrogen만이 산화적인 원인에 의한 신경세포사를 방어하는데 특이적인 효과를 가지고 있다. 본 연구는 천축황(天竺黃)의 항산화적 뇌신경 보호기전을 연구하는 것으로 신경세포주, 뇌세포막, 이의 산화적 정량실험법을 사용하여 천축황(天竺黃)이 갖는 항산화 및 신경보호활성이 소수성 페놀(phenolic molecules)성 물질과 유사함을 밝히게 되었다. 즉, 페놀성 물질로서 2,4,6-trimethylphenol, N-acetylserotonin, 및 5-hydroxyindole와 유사한 뇌신경 보호활성을 나타내었으며 천축황(天竺黃)은 생쥐의 N2a cell과 사람 SK-N-MC neuroblastoma cell에서 산화적인 글루탐산 독성에 대하여 보호를 하였다. 천축황(天竺黃)의 산화적 글루탐산 독성에 대한 보호활성은 과산화수소에 대한 것과 유사하였다. 이러한 항산화 활성은 $20\;{\mu}g/ml$에서, LDL의 산화적 보호 활성은 $5\;{\mu}g/ml$농도에서 발휘되었다 (최대활성은 $16\;{\mu}g/ml$). 이러한 결과는 천축황(天竺黃)이 노인성 치매에 보호효과가 있음을 시사하였다.

• Analytical Science and Technology
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• v.20 no.2
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• pp.176-182
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• 2007

The present study relates to a matrix-typed, sustained-releasing agent comprising agrochemical effective ingredients being capable of recognizing a content of moisture, and a preparation method thereof. The curdlan solution added to acid was formed matrix which has unique network structure. The matrix treated by heat lost water solubility. The network structure of matrix was opened in the aquatic condition but closed again in dry condition. Therefore, in the sustained-releasing formulation system, an agrochemical effective ingredient was released from the formulation only in the aquatic condition. Use of the composition according to the product can control a manifesting time of effects of agrochemicals and can provide agrochemicals with reduced harmful damages.

• The Korean Journal of Mycology
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• v.34 no.2
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• pp.92-97
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• 2006

The liquid culture extract of Coriolus versicolor was prepared by directly boiling the whole culture broth 7 days after incubation in 12% citrus extract medium. After removal of mycelial debris through filtration, this extract was further extracted with equal volume of ethyl acetate (1 : 1, v/v). The ethyl acetate extracts showed significant antibacterial activities against Stapylococcus aureus CCARM3230 and Psudomonas aeruginosa CCARM2171, which are resistant to several antibiotics. The most active fraction was eluted from a silica gel column with a mixture of dichloromethane and methanol (9 : 1, v/v) and the purity of this active substance was confirmed by HPLC analysis. The results suggest that the purified active substance could be a good source for the development of a new antimicrobial agent, especially for the treatment of antibiotic resistant bacteria.