The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antimicrobial Activity of the Coriolus versicolor Liquid Culture Extracts Against Antibiotic Resistant Bacteria and Purification of Active Substance

구름버섯 균사체 배양 추출물의 복합내성 세균에 대한 항균활성 및 활성물질의 정제

  • Lee, Jung-Sun (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Kim, Taeg (Department of Life Science, College of Natural Science, Cheju National University) ;
  • Lee, Yoon-Hi (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Jin, Cheng-Min (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Kim, Hyun-Guell (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Kim, Woo-Jung (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea) ;
  • Oh, Duek-Chul (Department of Life Science, College of Natural Science, Cheju National University) ;
  • Park, Yong-Il (Department of Biotechnology & The Biomaterials Engineering Center, Catholic University of Korea)
  • 이정선 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김택 (제주대학교 생명과학과) ;
  • 이윤희 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김성민 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김현걸 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 김우중 (가톨릭대학교 생명공학과 & 생물소재공학연구센터) ;
  • 오덕철 (제주대학교 생명과학과) ;
  • 박용일 (가톨릭대학교 생명공학과 & 생물소재공학연구센터)
  • Published : 2006.12.31
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Abstract

The liquid culture extract of Coriolus versicolor was prepared by directly boiling the whole culture broth 7 days after incubation in 12% citrus extract medium. After removal of mycelial debris through filtration, this extract was further extracted with equal volume of ethyl acetate (1 : 1, v/v). The ethyl acetate extracts showed significant antibacterial activities against Stapylococcus aureus CCARM3230 and Psudomonas aeruginosa CCARM2171, which are resistant to several antibiotics. The most active fraction was eluted from a silica gel column with a mixture of dichloromethane and methanol (9 : 1, v/v) and the purity of this active substance was confirmed by HPLC analysis. The results suggest that the purified active substance could be a good source for the development of a new antimicrobial agent, especially for the treatment of antibiotic resistant bacteria.

구름버섯 균사체를 12% 감귤 추출액에서 7일간 배양한 배양액을 열탕 추출한 후 균사체 잔사를 여과하여 제거하고, 동량의 ethyl acetate(1 : 1, v/v)로 재차 추출하였다. EA 추출물은 복합내성을 갖는 MRSA 균주인 S. aureus CCARM3230와 P. aeruginosa CCARM2171에 대해 현저한 생육저지 활성을 확인하였다. 본 추출물을 silica gel chromatography로 분획하였을 때 dichloromethane : methanol(9 : 1, v/v)의 용매조건으로 용출한 분획물의 항균활성이 가장 높았으며, 활성물질이 높은 순도의 단일 물질로 정제되었음을 HPLC 분석으로 확인하였다. 다제 내성 균주들에 대해 현저한 항균 활성을 보이고, 특히, 그람음성균인 P. aeruginosa CCARM2171과 그람양성균인 S. asures CCARM3230 균주 모두에서 현저한 항균활성을 나타낸 것은 본 추출물의 활성물질이 광범위 항균 스펙트럼을 갖는 신규의 항생물질로 개발될 수 있는 가능성을 보여주는 것으로 사료되었다.

Keywords

References

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