• The Microorganisms and Industry
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• v.19 no.1
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• pp.37-40
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• 1993

우리는 지금 경제적, 사회적, 기술적으로 매우 중대한 전화기에 처해 있다. 세계적으로는 기술보호주의와 국제적인 물질특허의 장벽을 넘어야함과 동시에 국내적으로는 건강한 삶, 깨끗한 환경, 풍요로운 사회건설을 통한 복짓회의 구현이 강력하게 요망되고 있기 때문이다. 이와같은 당면과제를 슬기롭게 극복하고 다가오는 21세기의 과학기술 선진국으로 진입하기 위하여는 여러가지 연구부문을 생각할 수 있겠지만, 특히 미생물 내지는 천연물로부터 다양한 대사기능을 갖는 새로운 생리활성 선도물질(lead moldecule)의 탐색연구가 매우 중요하리라 생각된다. 이는 국제적인 물질특허제도 등의 기술보호주의 장벽을 넘을 수 있음과 동시에 부가가치가 높고 세계시장을 확보할 수 있는 거대시장이 기다리고 있기 때문이다. 따라서 현재 정부정책 연구사업으로 추진하고 있는 G7 신기능 생물소재 연구 중 생리활성 선도물질 탐색기술 개발연구가 중과제로 선택된 것은 아주 시기적절한 결정이었다고 할 수 있다.

• Korean Journal of Plant Resources
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• v.9 no.1
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• pp.47-54
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• 1996

To search herbicidal compounds in Pulsatilla koreana Nakai, methanol extract of P. koreana roots was purified by sequences of XAD-7 column chromatography, silica gel adsorption column chromatography, silica gel flash column chromatography, preparative layer chromatography and preparative high performance liquid chromatography(Prep, HPLC).The final Prep. HPLC gave two herbicidally-active fractions. These fractions treatment at 100ppm inhibited the root length of Echinochloa crus-galli seedlings by 48% and 60% as compared with the control, respectively. Components in the two active fractions were analyzed by GC-MS Spectrometry. These compounds, which were isolated from P. koreana roots, were identified as several fatty esters, hydrocarbons, squalene, evidonol, and a diazepin analogue.

• Applied Biological Chemistry
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• v.41 no.2
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• pp.191-196
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• 1998

The MeOH extract from root of Pulsatilla koreana was showed antimicrobial activities against bacteria and yeast. The antimicrobial active substances of MeOH extract were successfully purified with solvent fractionation, silica gel adsorption column chromatography and Sephadex LH-20 column chromatography. The purified two active substances were isolated by HPLC and identified as 4-hydroxy-3-methoxycinnamic acid and 3,4-dihydroxycinnamic acid by MS, $^{1}H-NMR$ and $^{13}C-NMR$.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.94-101
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• 2000

On the purpose of development of antioxidative compounds from natural sources, 38 plants known to have antioxidant activity have been examined concerning DPPH radical scavenging activity. Among 13 plants exhibiting the activity, the seed of Carthamus tinctorius L, was selected as resources to search for active compounds due to rareness of study. The seed of the plant has been used as edible oil or preventive and remedial drugs for osteoporosis, arthritis. To reveal the principal component manifesting the antioxidant activity, the MeOH extracts was successively solvent-fractionated with EtOAc, n-BuOH and water. In order to isolate active component from the EtOAc fractions, application of silica gel column chromatographies and activity tests were repeated for a active component to be isolated. Its chmical structure was determined to be N-feruloylserotonin, a conjugated serotonin compound, by the interpretation of spectral data, NMR. MS and the adaptation of chemical reactions.

• Journal of the Korean GEO-environmental Society
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• v.4 no.2
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• pp.39-46
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• 2003

An immobilization zone can be constructed by modifying soils in the vadose zone with surfactants, which can be used to promote retardation of organic contaminants in the subsurface. Column experiments were conducted to investigate the behavior of surfactants and organic contaminants in unsaturated and saturated conditions with different water contents (25%, 50%, 75%, 100%). Transport and sorption of surfactant (dialkylated disulfonated diphenyl oxide) in the columns containing aluminum oxide was similar under the conditions at different water contents. However, transport of a model organic compound (naphthalene) was retarded as the water content decreased by enhanced partitioning of the compound into the surfactants that were sorbed on the aluminum oxide. This suggests that the immobilization method could well be applied to vadose zone as well as to saturated zone.

• Korean Journal of Food Science and Technology
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• v.35 no.4
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• pp.726-732
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• 2003

The crude extracts from Rhus javanica Linne showed comparatively strong antioxidative activity in test oils. Antioxidative components were isolated and identified by column chromatography, thin layer chromatography, UV, and NMR. These antioxidative components were added to several oils to compare antioxidative activity with several commercial antioxidants, such as BHA, BHT, and tocopherol. After the sixth column chromatography, one fraction (R-18-9-3-2-4-2) was separated from chloroform layer of Rhus javanica Linne. The R-18-9-3-2-4-2 fraction was identified as methyl gallate by $^1H-NMR$ and $^{13}C-NMR$ and confirmed with methyl gallate standard as authentic. The R18-9-3-2-4-2 fraction from chloroform layer of Rhus javanica Linne showed stronger activity than that of the ${\alpha}-,\;{\delta}-tocopherol$, BHT, and BHA at the same concentration.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.1-12
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• 2003

Myxobacteria are soil bacteria that move by gliding and have a complicated life cycle. In the research over the 25 years the myxobacteria have been shown to be a rich source of potentially useful bioactive substances. So far about 80 different basic compounds and 450 structural variants have been characterized. It is remarkable that myxobacteria produce the substance has special mechanisms. 26 new electron transport inhibitors,5 inhibitors of nucleic acid polymerases, 10 substances that act on the cytoskeleton, and 1 inhibitor of fungal acetyl-CoA carboxylase have been found. Presently, large-scale technical process was not fully established. But one of the compounds from myxobacteria is able to pass the many thresholds, which are on the road to application.

• Journal of Life Science
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• v.20 no.2
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• pp.269-274
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• 2010

An antifungal antibiotic-producing strain, BCNU 2003, was isolated from forest soil in Korea. The morphological and physiological characters, and 16S rRNA sequences analysis of strain BCNU 2003 identified this strain as Bacillus genus. The Bacillus sp. BCNU 2003 showed strong antifungal activities against Aspergillus niger, Trichophyton mentagrophytes and Trichophyton rubrum with inhibition ranging from 62.05 to 63.49% by using dual culture technique. Bacillus sp. BCNU 2003 produced a maximum level of antifungal substances under aerobic incubation at 28oC and pH 6.5-7.2 for 6 days in LB broth. Ethyl acetate extract of the cultured broth showed strong antifungal activity and a broad antifungal spectrum against various pathogenic fungi. The minimum inhibitory concentration (MIC) values for its active extracts ranged between 0.0625 mg/ml and 1 mg/ml. In addition, Bacillus sp. BCNU 2003 was determined to have the ability to produce enzymes such as amylase, protease, gelatinase and catalase.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.181-182
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• 2000

현재 의약품업계, 식품업계에서는 여러 가지 기능성 물질에 대한 관심이 집중되고있다. 이러한 기능성 물질은 항암, 항균, 항바이러스 및 식이섬유의 기능을 가지고 있어 부가가치가 높은 산물이다. 이들 기능성 물질중 $\beta$-1,3-D-glucan은 macrophage의 활성을 촉진시키고, 자외선에 의한 피부노화를 방지하며 생체내 free-radical을 제거하고, 특히 어류의 비특이 면역활성도 촉진시키므로 현재 양식업계에서 주목받고 있는 물질이다. 따라서 본 연구에서는 체외 다당류($\beta$-1,3-D-glucan)의 대량 생산을 위한 최적 배양 조건에 대하여 연구하였다. (중략)

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2001.05a
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• pp.240-241
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• 2001

자연계로부터 Vibrio sp.에 항균활성을 갖는 균을 검색하여 분리하고 분리균의 동정결과 Pseudomonas aeruginoso로 동정되었으며, 분리한 균의 항균성 물질의 최적 생산조건을 검토하며, 항균성 물질을 정제하여 그 안정성과 항균작용기작에 대해 조사한 결과 pH와 열에 대한 광범위한 안정성을 보였으며, 항균물질이 V. cholerae non-O1 ATCC 25872 세포에 대해 뚜렷한 용균작용을 하는 것을 관찰하였다.