• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.555-562
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• 2004

본 연구는 목저은 다음과 같다: 1) 돼지에서 150-kDa temary insulin-like growth faetor(IGF)complex의 한 구성 요소인 acid-labile subunit(ALS) 유전자 intron의 존재 확인. cloning 및 돼지 ALS(porcine ALS; pALS) complementary DNA(cDNA)의 3' 비해독(untranslated) 부위(3' UT) 증폭. cloning, 2) intron-spanning primer pair를 이용한 reverse transcription-polymerase chain reaction(RT-PCR) 방법에 의한 돼지 조직에서의 ALS 유전자 발현 분포 확인 및 3) 돼지 hepatocyte에서의 ALS 유전자 발현 여부 확인. 돼지 genomic DNA를 template로 하여 PCR 방법으로 예상된는 intron 부위를 증폭하고 plasmid vector에 삽입하여 염기서열을 결정한 결과 타 종의 ALS 유전자에서와 같은 위치에 1,371-base pair(bp)의 pALS intron이 존재함을 확인하였다. 역시 본 연구에서 간에서 추출한 RNA를 주형으로 시작하여 3' rapid amplification of cDNA end(3' RACE) 방법으로 147-bp의 3'UT를 합성하고 그 염기성열을 결정하였다. RT-PCR 결과 간은 물론 조사된 모든 돼지의 내장기관(신장, 폐, 비장)과 자성 생식기관(난소, 난관, 자궁) 및 골격근육에서 ALS 유전자가 발현됨이 밝혀졌다. 또한 돼지 간 조직에 대한 in-situ hybridization 결과 hepatocyte에서 ALS 유전자가 발현됨이 확인되었다. 이상의 결과는 ALS가 혈중 IGF의 저정/조절체로서의 주기능 외에 모세혈관 밖에서도 미지의 기능이 있을 기능성을 시사한다.

• Journal of Life Science
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• v.21 no.4
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• pp.534-541
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• 2011

Retinoic acid (RA), a metabolite of vitamin A, is known to regulate dedifferentiation of rabbit articular chondrocytes. The regulatory mechanism of dedifferentiation by RA is not yet understood. Thus, the effect of RA on the regulation of nitric oxide (NO)-induced dedifferentiation was investigated in rabbit articular chondrocytes. RA caused loss of the differentiated chondrocyte phenotype as demonstrated by inhibition of type II collagen expression and proteoglycan synthesis. RA also accelerated NO-induced dedifferentiation in rabbit articular chondrocytes as detected by expression of type II collagen and Sox-9 using Western blot analysis and production of sulfated proteoglycan using Alcain blue staining. Further, RA potentiated NO-induced activation of ERK. Inhibition of ERK with PD98059 (PD) recovered the expression of type II collagen and Sox-9 and production of sulfate proteoglycan in NO-induced dedifferentiated chondrocytes by RA treatment. Our findings suggest that RA accelerates NO-induced dedifferentiation of rabbit articular chondrocytes via the ERK pathway.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.13 no.2
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• pp.69-80
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• 2003

Verifiable encryption is an encryption technique with which one can verify what has been encrypted even if one can not decrypt the ciphertext. This technique can be used in fair exchange to convince the counterpart of his or her receiving an item by presenting an encrypted form in advance. In this paper, a method that can guarantee the payment atomicity is proposed by applying verifiable encryption to an electronic cash system based on the representation problem. With the new method, the process of dispute settlement is improved in the fact that the trusted third party do not have to interact with the bank to resolve disputes. This method is also flexible in a sense that clients and shops can request for dispute settlement regardless of any deadline constraint. However, additional proof is necessary to apply verifiable encryption during payment. We discuss the security and the atomicity of our method, and compare ours with others.

• Journal of the Korea Institute of Building Construction
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• v.22 no.6
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• pp.531-542
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• 2022

As buildings become taller, the strength of structural materials must increase and the amount of rebar reinforcement also increases while the application of an appropriate rebar splice method in the construction process become one of the essential factors. In this research, the current status of quality check for rebar coupler is identified by an expert questionnaire and an appropriate quality check method is proposed through a quality test on specimens similar to actual on-site construction. In the test results, it was revealed that, among the quality check methods for rebar coupler joints, the coupler tightening method and face to face degree of between upper and lower rebar did not affect the strength the specimen. Therefore, it is considered to be an appropriate way to check the coupler quality check to check whether the threads are completely assembled during on-site construction through manufacture the number of threads of rebar and coupler with a margin beyond the calculated thread number.

• Korean Chemical Engineering Research
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• v.61 no.1
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• pp.89-96
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• 2023

The Wiener-type nonlinear model where a static nonlinear block follows a dynamic linear block is widely used to describe the dynamics of chemical processes. A long process excitation step is typically needed to identify this Wiener-type nonlinear model with two blocks. In order to cope with this disadvantage, an identification method for the Wiener-type nonlinear model that uses only a single-step response is proposed here. The proposed method estimates the response of the dynamic linear sub-block from the initial part of the step response, and then the static nonlinear sub-block is identified. Because the only single-step response is used to identify the Wiener-type nonlinear model, there is great benefit in time and cost for obtaining process response. The performance of the proposed identification method with the single-step response is verified through a representative Wiener-type nonlinear process, a pH titration process, and a liquid level system.

• Journal of Veterinary Clinics
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• v.29 no.1
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• pp.82-86
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• 2012

A 5-year-old castrated male Chihuahua weighing 1.54 kg was examined because of a several month history of progressive right hind limb lameness. Physical examination of the stifle joints revealed pain and a grade IV medial patellar luxation on the right stifle joint. The right and left stifle joints were associated with a lameness of grade 2 and grade 0, respectively. Radiography revealed osteophytes or subchondral cystic lesions on the right and left stifle joints. Osteoarthrosis (OA) scores for the right and left stifle joints were 20 and 12 respectively. Combination of surgery and implantation of autologous adipose tissue derived mesenchymal stem cells (aAT-MSCs) was determined with informed consent. $1{\times}10^6$ aAT-MSCs suspended in PBS and 0.6 mL of hyaluronic acid were injected in the right stifle joint postoperatively. Osteoarthrosis scores and the lameness grade for the right and left stifle joints were 19 and 13, and 0 and 0 19 months after treatment, respectively, and 14 and 15, and 0 and 0 five years after treatment, respectively. This case report shows radiographical evidence of a decrease in osteophytes and subchondral cystic lesions on the stifle joint with OA after aAT-MSCs injection.

• Journal of KIISE:Computing Practices and Letters
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• v.5 no.4
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• pp.416-425
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• 1999

복잡한 무결성 제약 조건을 효율적으로 확인하기 위해 제약 조건들을 룰 베이스(rule base)에 저장하고 별도의 룰 관리 시스템과 제약 조건 관리 시스템을 통해 제약 조건을 확인하는 기법이 많은 연구자들에 의해 연구되고 발표되었다. 그러나 제약 조건 관리 시스템이 실행시간에 응용 프로그램을 항상 모니터링하고 있다가 데이타의 수정이 요청될 때마다 개입하여 프로세스를 중단시키고 관련 제약 조건을 확인하는 기존의 방법들은 처리 시간의 지연을 피할 수 없다. 본 논문은 컴파일 시간에 제약 조건 확인 코드를 응용 프로그램에 미리 삽입할 것을 제안한다. 응용 프로그램 자체 내에 제약 조건 확인 코드가 삽입되기 때문에 실행 시간에 다른 시스템의 제어를 받지 않고 직접 제약 조건의 확인 및 데이타베이스의 접근이 가능해져서 처리 시간의 지연을 피할 수 있을 것이다. 이를 위해 어떤 구문이 제약 조건의 확인을 유발하는 지를 추적하였고, 컴파일러가 그러한 구문을 어떻게 전처리 과정에서 검색하는지 그리고 그러한 구문마다 어떻게 해당 제약 조건 확인 코드를 삽입할 수 있는 지를 객체지향1) 데이타베이스 언어인 Objectivity/C++에 대해 gcc의 YACC 코드를 변경함으로써 보여 주었다.Abstract To cope with the complexity of handling integrity constraints, numerous researchers have suggested to use a rule-based system, where integrity constraints are expressed as rules and stored in a rule base. A rule manager and an integrity constraint manager cooperate to check the integrity constraints efficiently. In this approach, however, the integrity constraint manager has to monitor the activity of an application program constantly to catch any database operation. For each database operation, it has to check relevant rules with the help of the rule manager, resulting in considerable delays in database access. We propose to insert the constraints checking code in the application program directly at compile time. With constraints checking code inserted, the application program can check integrity constraints by itself without the intervention of the integrity constraint manager. We investigate what kind of statements require the checking of constraints, show how the compiler can detect those statements, and show how constraints checking code can be inserted into the program, by modifying the GCC YACC file for Objectivity/C++, an object-oriented database programming language.

• Food Science and Preservation
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• v.23 no.7
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• pp.977-988
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• 2016

The volatile flavor compounds in five Jeju citrus fruit varieties (Cheonhyehyang, Hallabong, Jinjihyang, Hwanggeum hyang and Redhyang) were extracted by simultaneous distillation-extraction (SDE) using solvent mixture of n-pentane and diethyl ether (1:1, v/v) and analyzed by using gas chromatography-mass spectrometry (GC-MS). The number of aroma compounds were identified with : 104 (4,939.77 mg/kg) in Cheonhyehyang, 98 (3,286.38 mg/kg) in Hallabong, 105 (3,317.56 mg/kg) in Jinjihyang, 102 (4,293.39 mg/kg) in Hwanggeumhyang, and 108 (4,049.94 mg/kg) in Redhyang. The detected main volatile compounds were; limonene, sabinene, ${\beta}$-myrcene, ${\alpha}$-pinene, ${\beta}$-pinene, linalool, 4-terpineol, ${\alpha}$-terpineol, (E)-${\beta}$-ocimene and ${\gamma}$-terpinene. Among the identified volatiles compounds, ethyl-benzene, nonanol, 1-p-menthen-9-al, (E)-isocarveol, methyl salicylate, ${\alpha}$-terpinen-7-al, perilla alcohol, and ethyl-dodecanoate were detected in Cheonhyehyang. only Furthermore, ${\beta}$-chamigrene and ${\alpha}$-selinene were in Hallabong only; 3-hydroxybutanal, (E)-2-nonenal, isoborneol, octyl acetate, (E)-2-undecenal, ${\beta}$-ylangene and guaia-6,9-diene in Jinjihyang. ${\rho}$-Cymenene, ${\beta}$-thujone, selina-4,11-diene and (E,E)-2,6-farnesol in Hwanggeumhyang only; and ${\rho}$-cymen-8-ol, bornyl acetate, carvacrol, bicycloelemene, ${\alpha}$-cubebene and 7-epi-${\alpha}$-selinene in Redhyang only. This study confirmed the differences in composition and content of volatile aroma components in five varieties of Jeju citrus fruits.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.14 no.2
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• pp.134-139
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• 2021

Currently, the autonomous vehicle market is commercializing a level 3 autonomous vehicle, but it still requires the attention of the driver. After the level 3 autonomous driving, the most notable aspect of level 4 autonomous vehicles is vehicle stability. This is because, unlike Level 3, autonomous vehicles after level 4 must perform autonomous driving, including the driver's carelessness. Therefore, in this paper, we propose the Emergency-notification and Driver-response Confirmation System(EDCS) for an autonomousvehicle safety that notifies the driver of an emergency situation and recognizes the driver's reaction in a situation where the driver is careless. The EDCS uses the emergency situation delivery module to make the emergency situation to text and transmits it to the driver by voice, and the driver response confirmation module recognizes the driver's reaction to the emergency situation and gives the driver permission Decide whether to pass. As a result of the experiment, the HMM of the emergency delivery module learned speech at 25% faster than RNN and 42.86% faster than LSTM. The Tacotron2 of the driver's response confirmation module converted text to speech about 20ms faster than deep voice and 50ms faster than deep mind. Therefore, the emergency notification and driver response confirmation system can efficiently learn the neural network model and check the driver's response in real time.

• Journal of Venture Innovation
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• v.7 no.1
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• pp.41-57
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• 2024

This study analyzed the relationship between the level of the venture environment, the benefits of the venture business verification system, internal capabilities, and management performance through the actual data of domestic venture companies collected in the Venture Business Precision Survey. As a result of the empirical analysis, the level of venture infrastructure did not affect the company's capabilities, but it was found that the venture company confirmation system had a positive effect. In the case of corporate competency, it was found that it had a positive effect on technological performance, but had no effect on sales performance. However, the research results confirmed that internal competency mediates the relationship between the venture environment level and the benefits of the venture business confirmation system and business performance. This study has the implications of confirming a series of processes leading to competency and performance in the environment using data from actual venture companies, and confirming the importance of corporate competency. In addition, since we confirmed the effect of the environmental factors of the venture environment level and the venture business confirmation system on the internal performance, if the process is introduced in the later reorganized venture business confirmation system, we will examine the factors that can enhance the performance of venture businesses. A more precise check is expected.