• Journal of Digital Convergence
• /
• v.14 no.5
• /
• pp.459-471
• /
• 2016

This study presents a comparative study for differences in efficacy and ingredient of white ginseng(WG), red ginseng(RG), and black ginseng(BG) using anti-oxidative activity test and anti-inflammatory activity test. In the results of cytotoxicity test for WG, RG, and BG, the survival rate of all cells was more than 95%. In the total polyphenol analysis, DPPH, ABTS radical scavenging test, and ROS production test, BG showed higher anti-oxidant activity than WG and RG. RG and WG showed higher inhibition activity of NO production and $PGE_2$ production, respectively. As results of the test for the effect on reduction of inflammatory cytokine production, WG and RG were effective on reduction of IL-$1{\beta}$ production, and BG was effective on reduction of IL-6. In the case of TNF-${\alpha}$ production, there was no difference among samples. This study could be useful basic data for the development of functional food and the fabrication of safe cosmetic.

• Journal of the Korea Convergence Society
• /
• v.8 no.12
• /
• pp.425-430
• /
• 2017

This study was conducted to measure the antimicrobial activity and antiseptic effect of Smilax china root extracts against Propionibacterium acnes and to develop natural antimicrobial extracts as an alternative for synthetic preservatives, which have recently been controversial. Extracts were obtained from dried Korean Smilax china root at room temperature using two solvents, distilled water and 95% EeOH, separately. According to the results of this study, the antimicrobial effect of Propionibacterium acnes 3314 and Propionibacterium acnes 3320 against Propionibacterium acnes was outstanding. In particular, the clear zone of the extract using distilled water showed the highest activity with 16.61 mm. As for the result of the collection of contaminated skin sample, the clear zone was 24.48 mm. This indicates that Smilax china root extracts show a high activity against skin flora as well as a high antimicrobial activity against Propionibacterium acnes. In conclusion, it is confirmed that Smilax china root extracts can be used as raw materials for cosmetics that have antimicrobial activity and antiseptic effect, and it is expected that Smilax china root extracts will be used as basic materials for the development of future natural preservatives.

• Food Science and Preservation
• /
• v.9 no.3
• /
• pp.345-350
• /
• 2002

This study was carried out to determine the effect of gamma irradiated Puerariae radix extract on color removal, antioxidative, DPPH radical scavenging and antimicrobial efftcts. Puerariae radix were extracted with methanol and acetone and irrdiated 10, 20 and 30 kGy with gamma ray. Hunter color L-value increased by irrdiation in a dose dependent manner, resulting in brighter color. But a and b values decreased by irradiation in a dose dependent manner. Antioxidant activities of the Puerariae radix extract in soybean oil emulsion were higher in methanol extract than acetone extract. Scavenging effect of Puerariae radix extracts on DPPH radical with methanol was not changed by irradiation but acetone decreased Acetone extract from Puerariae radix showed antimicrobial activities in B. subtilis, B, natto, B, megaterium S, aureus, Sal, typhymurium and E. coli methanol extract also had the antimicrobial activities but weaker for Sal. typhymurium and E. coli. Results suggested that Puerariae radix extracts have a potential as a natural food preservatives and cosmetic raw material.

• 한국생물공학회:학술대회논문집
• /
• 2003.04a
• /
• pp.475-479
• /
• 2003

Glycosaminoglycans(GAGs) from sea squirt, Styela clava was extracted with sodium phosphate at $105^{\circ}C$ for 2 hr and deprotein with trichloroacetic acid or hydrochloride. This GAGs was mainly constituted of galactose, glucosamine, glucose, mannose and galactosamine, and was phenylalanine, threonine, glutamic acid and aspartic acid. Mineral contents was mainly constituted 3.0mg% sodium, 1.6mg% potassium and 1.2mg% phosphorus and heavy metal was not detected. At pharmaceutical and cosmetic code of GAGs, protein and sulfate contents should included each range $14.0{\sim}22.0%$, $35.0 {\sim}45.0%$. After 5.0% trichloroacetic acid(w/v) and 10.0% HCl(v/v) treatment, protein and sulfate contents of GAGs was contained each 35.1%, 35.4% and each 22.0%, 18.5%.

• Applied Chemistry for Engineering
• /
• v.24 no.2
• /
• pp.126-131
• /
• 2013

BGsome coating on the surface of illite powder was attempted to impart skin affinity and improve various characteristics of the powder for makeup cosmetics. BGsome is a type of hydrated liquid crystalline vesicles prepared by the hydration of 1,3-butylene glycol (BG) dissolved lecithin. BGsome droplets were coated through wet process and the coated powders were analyzed by TGA, SEM, and particle size measurements. Effect of BGsome coating on the physical properties, such as flow-ability, dispersibility, spreadability, and adhesion, were examined. The repose angle of BGsome-coated illite was decreased, which means that BGsome coating improves the flowability of the powder. Dispersibility, spreadability, and adhesion of the coated illite were also improved remarkably. The result of adhesion tests showed that the BGsome-coated illite was evenly adhered onto artificial leather surface with almost no lumps. The flowability, dispersibility, spreadability and adhesion of face powders containing the BGsome-coated illite were also improved.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.04a
• /
• pp.124-124
• /
• 2003

해양 심층수는 저온 안정성, 부영양성 및 인공물질에 오염되지 않은 해양수로서 오늘날 많은 분야에서 사용 가능성이 대두되고 있다. 해양 심층수를 음료나 식품, 화장품 등에 이용하였을 경우 나타나는 효과의 일부는 미네랄 특성과 관계가 있는 것으로 알려져 있으며, 심층수를 원료로 한 음료수에는 혈행개선 효과가 있으며, 심층수를 생수로 섭취하였을 경우 혈중 콜레스테롤치을 유의적으로 낮추는 것으로 알려져 있으며, 간장의 인지질 값도 낮춘다는 연구보고가 있다. 또한 해양심층수는 아토피성 피부염에 효과가 있으며, 식품의 맛을 개선하는 것으로도 알려져 있다. 우리나라의 대표적인 음식인 김치는 배추, 무 둥을 식염으로 절여 각종 채소류 및 향신료를 첨가한 후 젖산발효를 적절하게 시켜 숙성한 채소발효식품으로 세계적인 음식으로서 각광받고 있다. 이 중 무는 천일염이나 정제염에 절여서 깍두기나 동치미 등에 사용되기도 하며, 김치의 재료로 배추 다음으로 이용율이 높은 김치소재이다. 무는 절임여하에 따라서 김치의 맛, 품잘, 미생물의 번식속도, 저장성 등이 변화하며, 절임이 중요한 품질요소로 알려져 있다. 소금의 종류 및 소금의 사용방법에 따른 무의 품질특성의 변화에 대한 연구들이 있으나 해양 심층수염을 이용하여 무를 절임시 품질특성에 대한 연구는 이루어져 있지 않는 실정이다. 이에 본 실험에서는 해양 심층수염에 무의 절임에 따라 무의 절임 특성 변화를 관찰하였다. 해양 심층수염과 천일염을 이용하여 무를 절임할 경우 수축율, 염도, 물성의 특성 변화를 관찰하였다. 해양 심층수염을 가지고 무를 절임할 경우 특성 변화를 살펴본 결과 수축률은 절임 8시간 이후에 해양 심층수염으로 절임한 경우가 천일염으로 절임한 경우보다 큰 것으로 나타났으며, 해양 심층수염이 천일염보다 더 높은 염도를 나타내며 절임되었다. 젤리강도는 절임시간이 증가함에 따라 감소하는 경향을 나타내었으며, 절임 염도가 높을수록 젤리강도는 낮아지는 경향을 나타내었다. 무의 연도(Softness)는 심층수염이 천일염보다 높은 값을 나타냄으로서 해양 심층수염에 절일 때 더 부드러운 물성을 나타냄을 볼 수 있었다. 견고성은 해양 심층수염에 절임하는 것이 천일염에 절임하는 것보다 더 높은 값을 나타났다. 해양 심층 수염이 천일염보다 빨리 무 조직에 손상을 줌으로 절임을 단축시키지만 절임시간이 연장되면 오히려 무 조직의 손상을 완화시키는 것으로 나타났다. 이러한 차이는 김치맛과 발효에도 영향을 미치는 품질 요인이 될 수 있을 것으로 판단되며, 해양 심층수염을 이용하여 김치발효시 특성 변화에 대해 더 구체적인 연구가 필요하리라 사료된다.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.04a
• /
• pp.147-148
• /
• 2003

최근 새로운 바다 자원으로서 “해양 심층수”가 주목받고 있다. 해양 심층수(深層水, deep sea water)는 태양광이 도달하지 않는 수심 200 m 이상 깊이의 물로 수온이 2$^{\circ}C$ 이하로 연중 거의 변화가 없는 저온안정성과 무기 영양염을 다량 포함하는 특징이 있어 심층수의 활용도는 매우 높다. NaCl만 제거하면 훌릉한 생수가 되기 때문에 심층수를 원료로 한 식품, 화장품, 의약품 개발에 대한 관심이 높아지고 있다. 미국, 일본 등지에서는 10여년전부터 심층수를 이용한 에너지, 수자원, 생수 및 식품 산업이 활발히 진행되어졌다. 현재 식품 산업분야로는 두부제조, 빵, 청주, 간장, 된장 등의 발효식품에 대한 연구가 진행되어지고 있다. 심층수를 적당하게 첨가하면 알코올 농도가 높아지는 발효강화작용이 있다. 새로운 건강 먹거리를 갈망하는 현대인들에게 해양 심층수를 이용한 발효식품은 매우 환영받을 것으로 사료된다. 이에 본 연구는 해양 심층수의 다양한 자원성을 효율적으로 활용하기 위해 심층수의 청정성과 풍부한 미네랄을 함유한 심층수의 농도에 따른 효모의 증식도를 알아봄으로서 적응력 강한 효모를 조사하였다. 실험에 사용한 해양 심층수는 淸凉飮料水(日本ナチュラルヘルス株式會社, 深透水 1550) 사용하였다. 효모균주는 경북과학대 전통식품 연구소에서 보유하고 있는 9종의 효모를 사용하였으며, 심층수 경도 250, 500, 1000의 심층수와 1차 증류수(대조군)에 sucrose 10% 첨가한 당용액을 사용하였다. 또한 당의 적응력을 알아보기 위해 sucrose 10, 15, 20% 첨가 당용액을 사용하여 Sacch.cerevisiae kluyvery DJ97 효모의 증식도를 알아보았다. 그 결과, Saccharomyces cerevisiae 12호 균주가 심층수 경도 500인 시험군에서 증식력이 높았으며, Saccharomyces cerevisiae 901 균주는 경도 1000인 시험군이 대조군에 비해 증식력이 높게 나타나 효모균주의 종류와 심층수의 첨가량에 따라 적응력이 차이가 있었다. 당농도와 심층수 첨가량에 따른 효모 균주의 발효력을 알아본 결과 심층수 경도 200의 당 10% 첨가군에서 가장 잘 증식하는 것으로 나타나, 알코을 효모균주의 발효력을 높이는데는 최적의 농도가 있음을 알 수 있었다. 따라서 심층수를 이용한 다양한 발효식품에 대한 연구가 요구되어진다.

• Applied Chemistry for Engineering
• /
• v.29 no.5
• /
• pp.524-532
• /
• 2018

In this study, two types of amino acids-based anionic biosurfactants such as potassium cocoyl glutamate (CTK) and sodium cocoyl glutamate (CTN) were synthesized from coconut oils and the structure elucidation of CTK and CTN was carried out by using FT-IR, $^1H-NMR$ and $^{13}C-NMR$ spectrophotometries. Measurements of interfacial properties such as static and dynamic surface tensions and emulsification activity showed that both CTK and CTN were surface-active and effective in lowering the interfacial free energy. In particular, the CTK surfactant was found to be more efficient in reducing the interfacial free energy since the larger number of CTK molecules was preferentially adsorbed at the air-water interface due to the higher hydrophobicity and larger mobility of CTK than those of using CTN, indicating possible uses in cosmetics and household products formulation.

• Applied Chemistry for Engineering
• /
• v.19 no.2
• /
• pp.179-184
• /
• 2008

The separation and purification of succinate are necessary for the succinic acid production by a fermentation process. Among the purification processes, desalination of succinate is inevitable. In this work, electrodialysis was selected as a desalination method and its operating parameters affecting the degree of desalination and energy consumption were examined. Commercialized electrodialysis apparatus was used in this work and its optimum operating parameters were determined by using design of experiment (DOE) method. Voltage, concentration of succinate, and pH were selected as main parameters. Among them, voltage seemed to be the most important one. The final conversion of succinate to succinic acid was calculated when the operating parameters were optimized. Finally, the effect of impurities, such as corn steep oil, yeast extract, and dextrose on the electrodialysis efficiency was also studied.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.32 no.1
• /
• pp.137-142
• /
• 2003

This study was carried out to examine the effects of gamma irradiation on the color removal, antioxidation, DPPH radical scavenger, and antimicrobial activity of Schizandra fructus extracts by different solvents. Schizandra fructus was extracted by hot water, ethanol, acetone and methanol, and the extracts were irradiated 10, 20 and 30 kGy with gamma rays. Hunter color L-value was increased by irradiation in a dose-dependent manner, resulting in brighter color for all solvents used. The a and b values were decreased by irradiation in a dose-dependent manner. Antioxidant activity of the ethanol extract of Schizandra fructus against soybean oil was the highest. The free radical scavenging activity was the strongest in hot water extract. All solvent extracts from Schizandra fructus had the strongest antimicrobial activities to B. subtilis, B. natto, B megaterium, S. aureus, Sal. typhymurium and E. coli. These results suggested that Schizandra fructus extracts have a strong potential as natural food preservatives and cosmetic raw materials. Furthermore, irradiation may not influence adversely on biological activites of the extracts when irradiated up to 30 kGy.