Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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The comparative study on physiological activity of White ginseng, Red ginseng and Black ginseng extract

백삼, 홍삼, 흑삼 추출물의 생리활성 비교 연구

  • 장아영 (중부대학교 한방건강관리학과) ;
  • 승윤철 (중부대학교 한방건강관리학과) ;
  • 지중구 (중부대학교 한방건강관리학과)
  • Received : 2016.04.08
  • Accepted : 2016.05.20
  • Published : 2016.05.28
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Abstract

This study presents a comparative study for differences in efficacy and ingredient of white ginseng(WG), red ginseng(RG), and black ginseng(BG) using anti-oxidative activity test and anti-inflammatory activity test. In the results of cytotoxicity test for WG, RG, and BG, the survival rate of all cells was more than 95%. In the total polyphenol analysis, DPPH, ABTS radical scavenging test, and ROS production test, BG showed higher anti-oxidant activity than WG and RG. RG and WG showed higher inhibition activity of NO production and $PGE_2$ production, respectively. As results of the test for the effect on reduction of inflammatory cytokine production, WG and RG were effective on reduction of IL-$1{\beta}$ production, and BG was effective on reduction of IL-6. In the case of TNF-${\alpha}$ production, there was no difference among samples. This study could be useful basic data for the development of functional food and the fabrication of safe cosmetic.

이 연구에서는 백삼(WG), 홍삼(RG) 흑삼(BG) 추출물의 효능차이를 비교하고자 항산화 및 항염 활성을 측정하였다. 세포독성 측정 결과 WG, RG, BG 모두 95% 이상의 세포 생존율을 나타내었고, 페놀함량, DPPH, ABTS 라디컬 소거능, ROS 생성량을 조사한 결과 WG와 RG에 비해 BG가 높은 항산화 활성을 보였다. NO생성 저해활성은 RG가, $PGE_2$생성 저해활성은 WG가 높았다. 염증성 사이토카인 생성량 감소에 미치는 영향을 측정한 결과, IL-$1{\beta}$ 생성량의 경우, WG와 RG가, IL-6 생성량의 경우 BG가 가장 큰 것으로 확인하였다. TNF-${\alpha}$ 생성량은 WG, RG, BG 모두 대조군과 비교하여 차이가 없었다. 이상의 분석 자료가 건강기능 식품과 기능성 화장품 원료 개발 등 전 임상단계의 기초적 자료로 가치가 있을 것으로 판단된다.

Keywords

References

  1. KY Nam," The comparative understanding between red ginseng and white ginsengs, processed ginsengs (Panax ginseng C.A. Meyer)", J. Ginseng Res. Vol. 29, pp. 1-18, 2005. https://doi.org/10.5142/JGR.2005.29.1.001
  2. Jae Eul Choi, Ki Yeul Nam, Xiangguo Li, Bok Young Kim, Hang Sik Cho and Kuang Bo Hwang., "Changes of Chemical Compositions and Ginsenoside Contents of Different Root Parts of Ginsengs with Processing Method", Korean J. Medicinal Crop Sci. Vol. 18, No. 2, pp. 118-125, 2010.
  3. Lee Jeehyun, Shen Guinan, Kim Euikeom, Shin Hyunjoong, Myung Changseon, Oh Hanjin, Kim Donghee, Roh Seongsoo, Cho Won, Seo Youngbae, Park Yongjin, Kang Cheolwoo, Song Gyuyong. "Preparation of Black Ginseng and its Antitumor Activit" ,Korean J. Oriental Physiology & Pathology., Vol. 20, No. 4, pp. 951-956, 2006.
  4. Gutfinger T., "Polyphenols in olive oils", Journal of the American Oil Chemists Society. Vol. 58, No. 11, pp. 966-968. 1981. https://doi.org/10.1007/BF02659771
  5. Boo, H. O., Lee, H. H., Lee, J. W., Hwang, S. J., and Park, S. U., "Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars",Korean J. Medicinal Crop Sci, Vol. 17, pp. 15-20, 2009.
  6. Jang E. K., Seo J. H. and Lee S. P., " Physiological activity and antioxidative effects of aged black galic(Allum sativum L.) extrac",. Kor. J. Food Sci. Thechnol. Vol. 40, pp. 443-448, 2008.
  7. Deuk Sang Kwon, "Changes in Ginsenoside Contents, Antioxidant and Anticancer Activities According to Steaming Condition in Ginseng. dissertation", Dankook University. 2012
  8. Ji Yeon Lee, Na Yeon Kim, Hye Lim Oh, Kun Jong Lee, Kee Heun Yang, Eun Soo Doh, Mee Ree Kim, "Antioxidant Activity of Rehmanniae Radix Preparata Prepared from Dried Root through Steaming-Drying Cycles", J. East Asian Soc. Dietary Life., Vol. 21, No. 6, pp. 838-843, 2011.
  9. Min-Ho Shin, "Comparison of ginsenosides and Anti-oxidant about Traditional Red Ginseng and High-hydrostatic Pressure Red Ginseng", dissertation. Kunsan National University. 2011.
  10. Rhee SG., "Cell signaling. H2O2, a necessary evil for cell signaling, "Science. Vol. 312, pp. 1882-1883, 2006. https://doi.org/10.1126/science.1130481
  11. Rhee SG, Bae YS, Lee SR, Kwon J., "Hydrogen peroxide: a key messenger that modulates protein phosphorylation through cysteine oxidation", Sci STKE. 2000:pe1. 2000. https://doi.org/10.1126/stke.2000.18.pe1
  12. Kalyanaraman B., "Teaching the basics of redox biology to medical and graduate students: Oxidants, antioxidants and disease mechanisms", Redox Biol. Vol. 1, pp. 244-257, 2013. https://doi.org/10.1016/j.redox.2013.01.014
  13. Bishop-Bailey D, Calatayud S, Warner TD, Hla T, Michell JA, "Prostaglandins and the regulation of tumor growth", J Environ Pathol Tox Oncol, Vol. 21, pp. 93-100, 2002.
  14. Son JH, Kim HJ, Park TS, Jung MS, "Study on the anti-oxidant and anti-inflammatory activities of sarcocarp and calyx of persimmon (Cheongdo Bansi)", J Appl Biol Chem., Vol. 54, pp. 71-78, 2011. https://doi.org/10.3839/jabc.2011.013
  15. Delgado AV, McManus AT, Chambers JP., "Production of tumor necrosis factor-alpha, IL-1${\beta}$, IL-2 and IL-6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides. Vol. 37, No. 6, pp. 355-361, 2003. https://doi.org/10.1016/j.npep.2003.09.005
  16. Jirik FR et al, " Bacterial lipopolysaccharide and inflammatory mediators augment IL-6 secretion by human endothelial cells", J Immunol. Vol. 142, No. 1, pp. 144-147, 1989.
  17. Chen F, Castranova V, Shi X., "New insights into the role of nuclear factor kappa B in cell growth regulation", Am J Pathol. Vol. 159, No. 2. pp. 387-397, 2001. https://doi.org/10.1016/S0002-9440(10)61708-7
  18. Lee AK et al,. "Inhibition of lipopolysaccharideinducible nitric oxide synthase, TNF-${\alpha}$, and COX-2 expression by sauchinone effects on I-${\kappa}$B${\alpha}$ phosphorylation, C/EBP and AP-1 activation", British Journal of Pharmacology. Vol. 139, pp. 11-20, 2003. https://doi.org/10.1038/sj.bjp.0705231
  19. Lee EH, Kim NK, Hwang CY, Kim HM. "Activation of inducible nitric oxide synthase byYongdam-SaganTang in mouse peritoneal macrophages", J Ethnopharmacol. Vol. 60, pp. 61-69, 1998. https://doi.org/10.1016/S0378-8741(97)00132-3