• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.202-212
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• 2004

The present study was conducted to compare of pollen morphological characteristics for five Haploxylon species in Korea using light microscopy(LM). The results are as follows; 1. Highly significant (P＜0.01) interspecific difference was observed in five Haploxylon species for their pollen morphological parameters. 2. The discreminant analysis based on the pollen morphological parameters demonstrated that the classification ratio of Haploxylon was 68.8 ％ ranging from 72.8 ％ of Pinus pumila to 62.2 ％ P. koraiensis. 3. The relationship among the species based on their pollen morphological parameters showed that P. koraiensis and P. pumila in Haploxylon were most closely related while P. pumila and P. bungeana were least related.

• KSBB Journal
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• v.17 no.6
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• pp.582-585
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• 2002

In an effort to use plant biotechnology for the production of biopharmaceuticals, pollens collected from lily (Lilium longiflorum) were grown in vitro and transformed with a PCR-amplified 1.7 kb cDNA encoding human tissue plasminogen activator (tPA) using Agrobacterium via a vacuum infiltration process. Western blotting showed that transgenic lily pollen tubes selected on kanamycin for 16 hrs expressed a tPA protein with a size similar to the human standard, suggesting their possible use as a disposable host for rapid foreign protein production.

• Proceedings of the KGS Conference
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• 2003.11a
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• pp.34-39
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• 2003

밀양강 중류부 충적평야의 화분분석 결과를 통하여 홀로세 후기 동안의 식생환경을 복원하고 한반도 남부지방의 농경의 기원과 확산에 대해 검토하였다. 경상남도 밀양시 산외면 금천리 단장천 충적평야에서 화분분석을 행하였다. 고고학 유적발굴을 위해 절개한 트랜치 T6(14.75-15.90m a.s.l. : 두께 1.15m)과 T9(17.00-15.60m a.s.l. : 두께 1.4m)의 노두 단면 중에서 비교적 유기질이 풍부한 실트층 및 토탄층에서 5cm등간격으로 채취하여 목본화분(AP: Arboreal Pollen)이 200개 이상이 될 때까지 검경하였다. (중략)

• The Korean Journal of Quaternary Research
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• v.20 no.1 s.26
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• pp.39-50
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• 2006

Jeonjangri site of Geochang area is located in the Geochang Basin, and lies on the river terrace of upstream part of Hwang River. Fluvial deposits are well distributed at the northern and southern walls of trench 2(district 2) in the Jeonjangri archeological site. This study aims to interpret the occurrences of fluvial sedimentary deposits on the basis of grain size analysis and palynological analysis in the representative sections of Jeongjangri site. The sedimentary profile shows that the upper units are typified by paleosols with soil wedge formed at about $25,000{\sim}30,000yr$ B.P, and the lower units are characterized by reddish brown muddy sands, organic muds and sand/gravel downwards in the profile. Particularly palynological study on the organic muds of southern wall section showed a result that lower unit is dominant with grass vegetation, and upper unit with Alnus-Quercus-Pinus vegetation. The former is interpreted to be formed at $60,000{\sim}50,000yr$ B.P (stadial), while the latter at $80,000{\sim}70,000yr$ B.P. In general broad-leaved/coniferous mixed forests are mostly dominant in Jeongjangri site and the climate was presumed to be cool temperate at that time.

• Korean Journal of Food Science and Technology
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• v.21 no.3
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• pp.404-408
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• 1989

This study was conducted to confirm amino acids from the filterate of pollen treated by purified enzyme of bee larva gut for improving pollen food. The larva gut enzyme were purified by ammonium sulfate, ethanol and Sephadex gel filtration. The filterate of pollen treated with enzyme purified by ammonium sulfate showed the highest amount of amino acids. The amount of amino acids of the filterate from which the pollen was fermented with natural contaminants is 5.5 times as much as the pollen which was not fermented. The filterate of pollen treated with enzyme of bee larva gut contained eighteen different amino acids as follows : aspartates, glutamate, serine, glycine, histidine, arginine, threonine, alanine, proline , tyrosine, valine, methionine, cysteine, isoleucine, leucine, phenylalanine, lysine and trytophane.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.8
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• pp.1353-1358
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• 2004

This study was conducted to increase crude protein and reducing sugar contents in pollen extracts by proteases. Four commercial neutral proteases (Alcalase 2.4L, Protamex, Flavozyme and Protease A) and two alkaline proteases (Protease S and Protease P) were used to prepare acorn and Darae pollen extracts. Contents of moisture, ash, crude protein and crude fat of acorn pollen were 5.2%, 2.7%, 6.2% and 22.3%, respectively, while those of Darae pollen were 5.4%, 2.8%, 1.8% and 27.8%, respectively. Contents of crude protein and reducing sugar in pollen extracts were increased by proteases. Alcalase 2.4L was the most effective in increasing protein contents while Protease A in increasing reducing sugar contents. It is suggested the use of proteases is one of the potential methods for increasing the contents of crude protein and reducing sugar in preparation of pollen extracts.

• Journal of the Korean Geographical Society
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• v.40 no.5 s.110
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• pp.571-583
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• 2005

This paper presents a paleoenvironmental study on Hoya San Nicolas, a maar lake in Valle de Santiago in Central Mexican Bajio. Maar lake sediments have been widely used for high-resolution reconstruction of paleoenvironment. Many different paleoenvironmental proxy data such as stable isotopes, pollen, and sediment chemistry were produced in this study. These data help to reveal paleoenviromental changes throughout the whole period covered by sediment materials from this study site. The evidence indicates that during ca. 11,000 - ca. 8,900 cal yr B.P. there was dry climate; during ca. 8,900 - ca. 7,000 cal yr B.P. it was wetter; during ca. 7,000 - ca. 4,000 cal yr B.P. drier; during ca. 4,000 cal yr B.P - the present wetter. Prominent dominance of Pinus pollen during ca. 11,000 - ca. 8,900 cal yr B.P. and during ca. 7,000 - ca. 4,000 cal yr B.P. may reflect very low lake levels resulting in poor preservation of pollen. Pinus pollen, the most resistant pollen type, may have been able to survive severe deterioration due to arid climate, but other pollen types may not. Due to likely droughts in these periods, a sedimentation gaps are probably present in the core.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.178-182
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• 2005

To identify the seedlings from controlled pollination between one paternal tree and three maternal trees of Japanese red pine, cpSSR markers of the paternally inherited haploid genome were analyzed in two year old 114 seedlings of full sib families. Individual specific DNA fingerprint like haplotypes of the parental trees were determined by PCR with three cpSSR primers. Haplotypes of the 114 seedlings were also identified by PCR with the same primers. On the basis of the comparison of cpDNA haplotypes of the 114 seedlings with those of the parental trees, 14 seedlings revealed to have distinguished haplotypes from those of the paternal tree. It was tentatively concluded that they were generated via pollination with the non-paternal trees. A seedling of Gangwon30 revealing non-paternal haplotype might have been generated via self pollination with the pollens of maternal tree through improper emasculation or contamination during artificial pollination. DNA fingerprint like cpSSR profiles observed in this study could be successfully applied to the various plant forensic analyses, such as identification of siblings of individual trees, asexually reproduced ramets of a specific clone, vegetatively propagated individuals via tissue culture, and pure full sib progenies.

• Journal of Food Hygiene and Safety
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• v.38 no.4
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• pp.202-210
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• 2023

To analyze the pesticide residues in commercial bee pollen products in South Korea, 61 samples were collected and screened for 339 pesticides. Results revealed that approximately 34% (>LOQ) of samples were contaminated with at least one pesticide. The pesticide residue detection rates of domestic and imported samples were 31% and 44%, respectively. Furthermore, the pesticide residue detection rate of online distribution (60%) was higher than that of offline distribution (27%). Fifteen pesticides were discovered in bee pollen, and pendimethalin, chlorfenvinphos, chlorpyrifos, and fluazinam were detected in 7, 6, 3, and 2 order of frequency, respectively. Even though its concentration was low, chlorfenvinphos which is banned in food crops in the United States, European Union, and Korea, was detected in bee pollen samples commonly. Therefore, continuous investigation of pesticide residues in bee pollen products and their acceptance criteria is required for safety.

• KSBB Journal
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• v.23 no.5
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• pp.460-462
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• 2008

Pollens grains collected from fully dehisced lily (Lilium longiflorum) anthers were given wounds by means of shaking in the presence of aluminum oxide particles. They were transformed by infiltration with Agrobacterium cells harboring a synthetic DNA encoding signal peptide-fused epidermal growth factor (EGF). After incubation for 24 hr in vitro, the pollen culture showed that EGF mRNAs and proteins were successfully expressed in the analysis of cDNA blot hybridization and immuno-blotting.