• Title/Summary/Keyword: 화분 분석

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The Effects of Soybean Cultivation on Soil Microorganism Activity (콩 재배가 토양 미생물 군집 활성도에 미치는 영향)

  • Bak, Gyeryeong;Lee, Gyejun;Kim, Taeyoung
    • Korean Journal of Environmental Agriculture
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    • v.38 no.2
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    • pp.76-82
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    • 2019
  • BACKGROUND: For sustainable agriculture, there are various agricultural practices including low input. Over the last few decades high input of chemical fertilizer and compounds results in environmental pollution and deterioration of soil fertility. Soybean (Glycine max L.) is well known eco-friendly crop due to their symbionts. Soybean has a relationship with nitrogen fixation bacteria called rhizobia. In this research work, we investigated effects of soybean cultivation on soil microorganism activities. METHODS AND RESULTS: Experiments were conducted in pots and potato cultivation was used as reference. Soil chemical properties were analyzed considering soil nutrient over cropping period. For the soil microbial community analysis, dehydrogenase activity analysis (DHA) analyzed along with denaturing gradient gel electrophoresis. The results showed that higher soil organic matter in the soybean cultivation soil than in the potato cultivation soil. Available $P_2O_5$ concentration increased gradually in both pots but showed higher value in the potato cultivation soil. DHA value implying microbial activities showed higher value in the soybean cultivation soil over all cropping period. CONCLUSION: The cause of high microbial activity in the soybean cultivation soil was considered to the effects of some specific microorganisms related to soybean cultivation. Therefore, the availability of soybean cultivation for sustainable agriculture should be encouraged in terms of microorganism community activity in soil.

Past Vegetation of Moor in Mt. Daeam in Terms of the Pollen Analysis (대암산 습원의 이탄의 화분분석에 의한 식생변천에 관한 연구)

  • Chang, Nam-Kee;Yung-Pok Kim;In-Hye O;Yung-Hi Son
    • The Korean Journal of Ecology
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    • v.10 no.4
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    • pp.195-204
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    • 1987
  • Pollen analysis of moor peat of Daeam Mountain revealed vegetational history around Daeam Mountain. The pollen stratigraphy might be zoned into three, Zone I, Zone II and Zone III for the past 2, 200 years. The time required for amount of the accumulated peat in the moor was estimated in terms of the balance of the accumulation and decay of organic carbon of the deposit peat. Zone I(770~2, 200 yr BP) had Pinus and Quercus as main elements. Gramineae decreased while Typhaceae, Cyperaceae and spores such as Polypodiaceae increased. Zone II(300~770 yr BP) showed warming trend which is suggested by high pollen concentrations of Quercus, Juglans, Carpinus, Ulmus and by a more diverse flora of deciduous borad-leaved trees than Zone I. Pinus decreased in this zone. It suggests that the overall environment became milder than Zones I and III. Zone III(earlier than 300 yt BP) was predominantly Quercu and Pinus with amount of nonarboreal species such as Artemisia, Gramineae and Chenopodiaceae, that was grown under dry conditions. It suggests that overall environment vecome humid by comparison with the present time and it is surmised the course of boggy ground formation by a retrogressive succession.

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Investigation of Antidiabetic Medicinal Plants using an Oriental Medicinal Database (동양의학 데이터베이스를 이용한 당뇨병에 효능을 가진 생약재의 탐색)

  • Kim, Dong-Hyun;Kang, Young-Gu;Kim, Hong;Chae, Hee-Jung
    • KSBB Journal
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    • v.19 no.2
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    • pp.125-131
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    • 2004
  • Various medicinal plants were examined as resources for antidiabetic biomaterials, using an oriental medicinal database. The prescription frequency and dosage of medicinal plants in 131 antidiabetic prescriptions were analyzed. Total prescription score of each medical plant was summed up from prescription table and score table. High-scored medicinal plants have been widely studied in the previous research on the diabetic treatment. Consequently, dangui, hwangryun, maekmoondong, ginseng, chick, saengjihwang, omija, bokryung, Jimo and cheonhwaboon were assumed to have high antidiabetic activities.

The Geomorphic Characteristics and Late-Holocene Paleoenvironmental Reconstruction in Sejong-Si Based on the Pollen Analysis of the Right Bank of Geumgang (세종시 금강 우안(右岸)지역의 지형특성과 홀로세 후기 고환경복원)

  • Moon, Youngrong;Yoon, Soon-Ock;Hwang, Sangill
    • Journal of The Geomorphological Association of Korea
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    • v.24 no.4
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    • pp.1-12
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    • 2017
  • This study tried to reconstruct paleovegetation environments during the late Holocene with pollen analysis from archeological trenches at the right bank of Geumgang in Sejong-si. The lower hills was dominantly covered with pine trees, while alder trees and hygrophytes extended on the floodplain since 2,000yr BP. The pollen composition of Alnus dominance in the floodplain during the times is different from the general charateristics of Korean peninsula. Such a phenomenon is thought to be influenced by the fluvial process on the floodplain in the middle reaches of Geumgang around Sejong-si. Mihocheon and some tributaries join to Geumgang around Sejong-si, and the channel of Geumgang becomes narrow around the boundary between Sejong-si and Gongju-si. This narrow channel could attribute to the formation of extensive floodplain around Sejong-si. Moreover, human impacts such as agriculture appear continuously since 1,800yr BP.

New Taxonomic Treatment of Lycoris koreana Nakai (백양꽃(Lycoris koreana Nakai)에 대한 분류학적 재검토)

  • Lee, Jong-Won;Chung, Kyong-Sook;Kang, Shin-Ho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.21-21
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    • 2019
  • 백양꽃(Lycoris koreana)이 속해 있는 상사화속(Lycoris)은 종간 잡종이 쉽게 일어나 많은 자연교잡종을 가지고 있어 식물의 종분화와 진화를 연구하는데 흥미로운 분류군이다. 그러나 이런 사유로 명백한 분류학적 차이가 있음에도 불구하고 대상종의 분류학적 위치의 설정에 문제가 발생되어 계급의 설정에 혼란을 가져오기도 한다. 특히 한국산 상사화속의 종 분류는 그동안 많은 형태학적, 분계학적, 화분학적, 세포학적, 분자생물학적 연구가 이루어져 있음에도 아직도 도감과 많은 문헌에서 오류와 오동정의 문제점을 가지고 있다. 특히 백양꽃에 대하여서는 Nakai에 의해 신종으로 설정된 이래 일본에 분포하는 동 속 식물과의 관계 분석의 오류로 계급의 설정에 현재까지 다양한 문제가 발생되고 있다. 백양꽃은 전라남도 백양산에 분포하고, 포가 자주색이고, 꽃의 크기가 작으며, 화사와 화주가 적색 또는 암적색인 특징으로 Nakai에 의해 신종으로 설정되었다. 이후 Nakai와 Lee는 L. koreana 가 한국에만 분포하는 것으로 보고 한국 특산종으로 취급하였다. 그러나 백양꽃의 실체에 대하여 많은 논의가 있었으나 Lee and Oh는 L. radiata로 동정하였고, Yang은 L. koreana는 한국에 분포하지 않고 L. sanguinea만이 분포하는 것으로 보고하였다. Koyama는 L. sanginea에 대하여 L. sanguinea와 L. kuishiana를 비교하여 수술이 화관 밖으로 돌출되어 있는 점 외에는 L. koreana와 차이가 없음을 들어 각각 L. sanguinea var. sanguinea, L. sanguinea var. kuishiana 그리고 L. sanguinea var. koreana로 재설정 하였고 이는 한국과 일본학자들에 의해서도 거의 받아들여지고 있으나 본 연구를 통하여 L. koreana의 종(species)으로의 계급 부여와 한국특산식물 설정의 타당한 결과를 도출하였다.

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A Study on the Neoasozine Residues in Rice Grain by Neutron Activation Method (방사화(放射化) 분석법(分析法)에 의한 미곡(米穀)중 네오아소진 잔류분(殘留分)에 관한 연구(硏究))

  • Kim, Yong-Hwa;Lee, Koon-Ja;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.13 no.1
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    • pp.20-24
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    • 1981
  • Residues of neoasozine in rice grain were determined by neutron activation and colorimetric techniques. Twice application of the chemical before flowering did not lead to any increased residue level while 4-times application resulted in significant increase in the residue level up to 0.54-0.75 mg $As_2O_3/kg$. The partition ratio of arsenic residues into polished rice grain and bran was 73 : 27 in 100% polishing while most of the residues in the bran was transferred to oil cake fraction during solvent extraction, reaching up to 2.9 mg $As_2O_3/kg$. The neutron activation technique was advantageous because of its high sensitivity and the smaller sample amounts required for analysis.

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Genetic Stability Analysis of in vitro Regenerated Wolly Grass (Imperata cylindrica 'Rubra') Based on Inter-Simple Sequence Repeats (ISSR) Markers (ISSR 표지에 의한 기내재생 홍띠(Imperata cylindrica 'Rubra')의 유전적 안정성 분석)

  • Ye-Jin Lee;In-jin Kang;Chang-Hyu Bae
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.54-54
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    • 2020
  • 지구온난화에 따라 농업부문 신재생에너지의 중요성이 증대되고 있으며, 화본과 식물은 바이오에너지작물의 중요한 소재를 제공하고 있다. 화본과 식물의 기내대량증식연구의 일환으로 홍띠식물의 기내 재생 식물체의 유전적 안정성에 대한 기초자료를 제공할 목적으로 기내배양으로 재분화시킨 홍띠(Imperata cylindrica 'Rubra') 재분화 식물체 중 녹색체 재생식물체를 대상으로 ISSR 표지를 사용하여 유전적 안정성을 조사하였다. 재분화식물체는 MS (Murashige and Skoog, 1962)배지에 생장조절제를 첨가한 배지에서 배양하였다. 생장점 부위를 적출하여 캘러스를 유도하고(0.1 mg/L 2,4-D와 2 mg/L BA), 캘러스 증식(0.1 mg/L 2,4-D와 0.05 mg/L BA), 신초 재분화( 0.01 mg/L NAA와 2 mg/L BA) 후 MS배지에서 식물체를 양성하고 순화시켰다. 배양은 26±2℃, 25 µmol/m2/s, 14h/10h (day/night) 광조건 하에서 실시하였다. 재분화식물체는 홍띠 및 녹색 재분화식물체 2 종류로 나타났는데, 이는 생장점에서는 홍띠가 분화되었음에도 불구하고 생장점 주변조직에서 유래한 녹새체가 분화된 후 우세하게 자라서 녹색재생체가 우점하는 것으로 추정된다. ISSR 분석은 대조구로 모식물체 홍띠를(8개체), 재분화식물체는 녹색체 중, 1년간 노지포장에서 재배중인 녹색체(10개체)와 실험실내 화분에서 재배중인 시료를(10개체) 사용하였다. ISSR 밴드패턴을 비교한 결과, 재분화체는 실내포트 재배식물체 10.3%, 노지1년 재배식물체 8.3%로 대조구의 4.1%보다 유전적 다형성 비율이 2배 이상 높게 나타났다. 또한 재분화식물체들의 유전적 유사도를 평가하고 군집분석을 실시하였다.

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Two-Year Estimates of Mating System in Seed Orchard of Pinus densiflora Revealed by cpSSR and nSSR Markers (안면도 소나무 채종원 교배양식 추정모수의 연간비교)

  • Kim, Young Mi;Hong, Yong Pyo;Park, Jae In
    • Journal of Korean Society of Forest Science
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    • v.104 no.4
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    • pp.578-587
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    • 2015
  • Nuclear SSR (nSSR) and chloroplast SSR (cpSSR) markers were analyzed to assess the parameters of mating system in seed orchard, such as outcrossing rates, the number of potential pollen contributors, paternal contribution rates, degree of pollen contamination, and biparental inbreeding ($t_m-t_s$). In 2006, 2007, seeds were collected from the seed orchard of Pinus densiflora, established in 1977 at Anmyeon island. Estimates of outcrossing rates ranged from 94.9 to 100% (mean 98.9%) in 2006 and from 91.2 to 100% (mean 97.7%) in 2007 on the basis of the analysis of cpSSR haplotypes and from 90.3 to 100% (mean 95.9%) in 2006 and from 81.6 to 100% (mean 95.3%) in 2007 on the basis of the analysis of nSSR genotypes. By cross checking of both DNA markers, mean cumulative outcrossing rates of 100% and 98.9% were estimated in each year. Mean contamination rates were estimated as 48.9% and 42.4%, respectively. On the basis of cpSSR haplotype observed in each seed, paternal contribution rates (the number of pollen contributors) were estimated as 0.458 (mean 16.2) in 2006 and 0.512 (mean 14.8) in 2007. In conclusion, considering pretty high level of outcrossing rates observed in a seed orchard, there might be little to be influenced by inbreeding depression for genetic potential of the seeds induced by selfing. Estimates of the mating system parameters obtained from the two reproductive years were not statistically different, which revealed stable genetic quality of seeds produced in different years. Observed results from this study may provide useful information for the management and establishment of the seed orchard of the progressive generation.

The chemical speciation and analysis of trace elements in sediment with neutron activation analytical method(NAA) and atomic mass spectrometry (중성자 방사화분석법과 원자질량분석법을 이용한 침전물 시료속에 존재하는 미량원소들의 화학종 분류 및 분석)

  • Nam, Sang-Ho;Kim, Jae-Jin;Chung, Yong-Sam;Kim, Sun-Ha
    • Analytical Science and Technology
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    • v.16 no.5
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    • pp.358-367
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    • 2003
  • In this research, first of all, the analytical methods for the determination of major elements in sediment have been developed with ICP-MS (Inductively Coupled Plasma Mass Spectrometry). The analytical results of major elements (Al, Ca, K, Fe, Mg) with Cool ICP-MS were much better than those with normal ICP-MS. The analytical results were compared with those of NAA (Neutron Activation Analysis). NAA were a little superior to ICP-MS for the determination of major elements in sediment as a non-destructive trace analytical method. The analytical methods for the determination of minor elements (Cr, Ce, U, Co, Pb, As, Se) have been also developed with ICP-MS. The analytical results by standard calibration curve with ICP-MS were not accurate due to the matrix interferences. Thus, the internal standard method was applied, then the analytical results for minor elements with ICP-MS were greatly improved. The analytical results obtained by ICP-MS were compared with those obtained by NAA. It showed that the two analytical methods have great capabilities for the determination of minor elements in sediments. Accordingly, the NAA will play an important role in analysis of environment sample with complex matrix. ICP-MS also will play an important role because it has a great capability for the determination of Pb that could not be determined by NAA.

The Study for the Optimal Location of Fire Stations in Seoul (서울시의 소방서 최적입지에 관한 연구)

  • Kim, Geun-Young;Kang, Sung-Il
    • Journal of the Korean Society of Hazard Mitigation
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    • v.2 no.1 s.4
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    • pp.153-159
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    • 2002
  • Disasters are the phenomena that we have to prevent the occurrence in order to keep the safety to human lives and properties, and if occurred, we have to minimize the economic, social, and mental costs of the occurred disasters or incidents. This research analyzes the optimal location of fire stations in terms of served population maximization in Seoul. This research introduces "the maximal covering location problem(MCLP)," one of the optimization techniques, as the primary research method. This research also applies a geographic information system into spatial distribution analyses of existing fire stations and observed fire incidents. Results from the analyses show that the existing location of fire stations and branches need to be improved. The dispatch location of fire engines should be reconsidered for rapid services of fire fighting.