• Title/Summary/Keyword: 혼합 추출물

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Bacterial Chemotaxis to Extracts, Exudates, Solutions in Vitro and Soil (In vitro 및 토양에서 추출물, 삼출물, 용액에 대한 세균의 화학주성)

  • Lee Min Woon;Kim Sung Ill;Shim Jae Ouk;Shin Hyun Sung;Kim Gwang Po
    • Korean Journal Plant Pathology
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    • v.2 no.3
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    • pp.165-173
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    • 1986
  • Accumulation of Pseudomonas sp., P. fluorescens and Erwinia carotovora in 60 min treatment was greater in extracts from soil, exudate from ginseng root and solutions than distilled water. In bacterial movement toward rubber tube soil from chamber, accumulation of P. fluorescens in response to soil supplemented with soil extracts, exudate and solutions was generally greater in soil extracts compared to control and other solutions, but Pseudomonas sp. and E. carotovora were not much response to supplemented extracts, exudate and solutions. Accumulation of the bacteria in capillaries containing various exudates from fungal propagules with not attracted to the exudates. For an accumulation of bacteria in rubber tubes containing soil inoculated with fungal propagules, the Pseudomonas sp. was not attracted in soil inoculated by the organisms as attractant but P. fluorescens and E. carotovora to fungi were attracted to F. solani, F. oxysporum and mixed organism Alternaria panax did not affect on bacterial movement except E. carotovora. The organic matter conten in Kangwha and Kimpo soil were low in diseased and healthy soil. The K content was especially high in Kimpo healthy soil. Bacterial population in Goesan and Kangwha were more abundant than other soil. The number actinomycetes was populated abundant in healthy soil of Goesan and diseased soil of Poonggi.

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Recovery Effect of Garlic Extract and Vitamin B group Enhanced Diet on Swimming Fatigue (수영부하 피로에 대한 마늘추출물 및 비타민 B군 강화 급이의 회복 효과)

  • Kang, Min-Jung;Shin, Jung-Hye;Sung, Nak-Ju
    • Journal of Life Science
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    • v.21 no.6
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    • pp.875-883
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    • 2011
  • The purpose of this study was to investigate the effects of a diet supplemented with garlic extract and vitamin B complex on fatigue recovery from swimming load test in rats. Sprague-Dawley rats were divided into a non-swimming control group (Normal) and swimming load test groups. Swimming load test groups were re-divided into swimming control group (Control), 1% of 60 brix garlic extract fed group (SG), vitamin B complex twice mixture fed group (SV) and 1% of 60 brix garlic extract and vitamin B group twice mixture fed group (SGV). Concentration of plasma lipids and glucose were determined in the rats post-swimming. Concentration of plasma glucose, total lipid, total cholesterol and triglyceride were significantly increased in the control group compared to the non-swimming control group (p<0.05), and these levels were shown to be significantly lower in the SG, SV and SGV groups than in the control group. The SGV group showed the lowest level, however, there was no significant difference between the SG and SV groups. There was no significant difference in HDL-cholesterol concentrations between the normal group and control group, but its concentration was the highest in the SG ($45.95{\pm}5.52$ mg/dl) group. LDL-cholesterol concentration was the highest in the control group, but it was decreased significantly in the SGV group. In conclusion, this study demonstrates that a diet supplemented with 1% 60 brix garlic extract and vitamin B group twice mixture has beneficial effects on fatigue recovery in swimming load tests.

Weight Loss Effect of Wax Gourd (동아의 체중조절 효과)

  • 홍석산;이승환;김철용;권석형;황보식
    • The Korean Journal of Food And Nutrition
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    • v.15 no.4
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    • pp.289-294
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    • 2002
  • This study attempted to evaluate the effectiveness of function of Wax Ground for obesity and the related disease of adult people can be developed by the combination of the 17 food materials screened in this study. The experiments using Sprague-Dawley male rats show that seventeen kinds of food material inhibited the increase of body weight gain, energy intake, fat pads weight, liver triglycerides content, atherogenic index, and blood glucose concentration induced by cafeteria diet. Oral administration of wax gourd decreased most body weight gain, energy intake, fat pads weight, and blood glucose concentration. Liver triglycerides content was the lowest in the group administered mulberry stem extract and the group administered pine leaves showed the lowest atherogenic index. The combined administration of wax gourd, mulberry stem extract and pine leaves improved more the obesity and related symptoms than the individual administrations.

Flavonoid Acetylated Glucosides of the Needles of Pinus densiflora (솔잎의 후라보노이드 아세틸화 배당체)

  • Lee, Sang-Keug;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.29 no.4
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    • pp.48-52
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    • 2001
  • The needles of Pinus densiflora, grown in the campus of Kangwon National University were collected, extracted with acetone-$H_2O$ (7:3, v/v) and freeze dried to give 10.4 g of ethylacetate soluble powder. A portion of the freeze dried powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol and ethanol, and ethanol-hexane mixture as eluents. The extractives contained a large amount of flavan compounds in addition to a small amount of flavonoid derivatives such as kaempferol-3-O-glucopyranoside, and quercetin-(6"-O-acetyl)-glucopyranoside which has not been isolated from this species yet. Some spectrometric analyses such as NMR and FAB-MS were performed to identify the structures of the isolated flavonoid derivatives.

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Dangerous Abandoned Object Extraction Model Using Area Variation Characteristics (면적의 변화 특성을 이용한 위험 유기물 형상 추출 모델)

  • Kim, Won
    • Journal of the Korea Convergence Society
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    • v.11 no.8
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    • pp.39-45
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    • 2020
  • Recently the terrors have been attempted in the public places of the nations such as United states, England and Japan by explosive things, toxic materials and so on. It is understood that the method in which dangerous objects are put in public places is one of the difficult types in detection. While there are the cameras recording videos for many spots in public places, it is very hard for the security personnel to monitor every videos. Nowadays the smart softwares which can analyzing videos automatically are utilized to detect abandoned objects. The method by Lin et al. shows comparatively high detection rates for abandoned objects but it is not easy to obtain the shape information because there is a tendency that the number of the pixels decreases abruptly along the time goes due to the characteristics of short-term background images. In this research a novel method is proposed to successfully extract the shape of the abandoned object by analysing the characteristics of area variation. The experiment results show that the proposed method has better performance in extracting shape information in comparison with the precedent approach.

Separation and Performance lest of Wnitening Agent in Rhodiola Sachalinensis (홍경천에 포함된 미백성분의 분리 및 성능검사)

  • 최두영;안소영;이승기;한정선;김은철;이향복;신정현;김은기;노경호
    • KSBB Journal
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    • v.19 no.3
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    • pp.169-173
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    • 2004
  • The Rhodiola Sachalinensis 5 g were mixed and extracted with methanol 150 $m\ell$ at the room temperature for 12 h. The effluents were collected and grouped into the two. Un this experimental condition, the mobile phase composition were linearly changed as follows; water/methanol : 90/10 - 30/70 (vol. %, for 5 min), 30/70 - 10/90 (vol. %, for 15 min) and an analytical column (3.9 ${\times}$ 25 em, 15 $\mu\textrm{m}$ particle size, and 300 ${\AA}$ pore size) was utilized. The performance of the extracted Rhodiola Sachalinensis as a whitening agent was not favorable, so it classifies the Rhodiola Sachalinensis extractions with two fractions and collects each fraction for whitening agent assay. For the in-vivo melanin production ratio assay that used melanin-a cell in 10 ppm concentration, it was 58.6%, the first fraction of the effluents collected between 1.0 and 4.0 min, while it was 60% between 10.4 and 17.6 min for the second fraction, which were more efficient than that of arbutin, 45.6%.

Food Quality Comparison of Dried Persimmons (Diospyros kaki THUNB) When using Medicinal Plant Extracts and Food Additives during Drying Process (약용식물 추출물 및 식품첨가제가 곶감 식품학적 품질 비교)

  • Kim, Ki-Ho;Kim, Kyung-Min
    • Current Research on Agriculture and Life Sciences
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    • v.32 no.1
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    • pp.10-17
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    • 2014
  • This study presents an eco-friendly persimmon drying system to satisfy consumer preferences and provide a popular food for both the domestic and international markets. The most effective antimicrobial compounds were developed from a combination of plant extracts (18.18% clove buds, 9.90% cinnamon, 9.09% licorice, 4.55% cnidium, 4.55% seed of grapefruit, and 54.54% apple vinegar). The dried persimmons were evaluated as regards their moisture and sugar content, weight, hardness, and color value. During the drying process, the overall moisture content of the persimmons increased, along with the sugar content. The hardness was almost the same for each region and decreased on an average of 0.5~0.86 after 6 weeks. As regards the chromaticity, ${\Delta}E$ decreased during the drying process, while L-value became darker and a-value showed a dark red color over time.

Analysis of Chemical Constituents of Agastachis Herba and in silico Investigation on Antidiabetic Target Proteins of its Major Compounds (곽향의 성분 분석 및 주요 성분들의 in silico 항당뇨 타겟 단백질 탐색)

  • Choi, Jongkeun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.4
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    • pp.483-492
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    • 2021
  • Agastachis Herba (AH) to treat anorexia and nausea and its antidiabetic efficacy was recently reported. This study examined the antioxidant activities and chemical constituents of AH and predicted the target proteins of each compound using in silico approaches. The results showed that EC50 values of AH methanol extract for DPPH and ABTS radical scavenging were 78.6 ㎍/mL and 31.0 ㎍/mL, respectively. Compared to the EC50 values of ascorbic acid (9.9 ㎍/mL, 5.2 ㎍/mL), the AH methanol extract possessed excellent antioxidant activities. Rosmarinic acid, tilianin, agastachoside, and acetin were confirmed as the major compounds of extracts by qualitative analysis performed with HPLC-PDA-MS/MS. The antidiabetic target proteins of these compounds were predicted by applying a structural similarity and inverse docking methodology using a DIA-DB server. The resulting target proteins were PPAR-γ, DPP IV, glucokinase, α-glucosidase, SGLT2, aldose reductase, and corticosteroid 11-beta-dehydrogenase, some of which have already been proven experimentally as target proteins. Therefore, the in silico methods can be considered valid. Finally, AH were extracted with various solvents to determine the optimal conditions for the extraction of active components. Methanol among organic solvents and 80% ethanol in ethanol-water mixtures were identified as the most effective solvent for the extraction.

Screening for Antioxidative Activity of Crude Drugs (항산화성(抗酸化性) 생약(生藥)의 선발(選拔))

  • Kim, Seung Yeol;Kim, Jin Hwan;Kim, Seung Kyeom
    • Korean Journal of Agricultural Science
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    • v.19 no.1
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    • pp.103-114
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    • 1992
  • Screening for antioxidative activities of 180 species of crude drugs were performed on their methanol extracts. More than 45% of those showed some effect on oxidative stability of linoleic acid, and 44 species seemed to have rather strong antioxidative activity. Selected these samples of the active crude drugs were further examined in their methanol extracts with methyl linoleate emulsion system. especially 11 species revealed strong antioxidative activity. These 11 species were then successively extracted with ethyl acetate and petroleum ether, and their antioxidative activity was determined. The ethyl acetate and petroleum ether extracts of Epimedium Koreanum NAKAI, Psoralea Corylifolia L., Syringa Dilata NAKAI and Prunus mume Sieb, et Zucc. showed much more effective than the others in stabilizing methyl linoleate. Scutellaria baicalensis George. Glycyrrhiza glabra L. were only effective in the methanol extract.

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A Study of Optimal Lotion Manufacturing Process Containing Angelica gigas Nakai Extracts by Utilizing Experimental Design and Design Space Convergence Analysis (실험 설계와 디자인 스페이스 융합 분석을 통한 Angelica gigas Nakai 추출물을 함유한 로션 제조의 최적 공정 연구)

  • Pyo, Jae-Sung;Kim, Hyun-Jin;Yoon, Seon-hye;Park, Jae-Kyu;Kim, Kang-Min
    • Journal of Convergence for Information Technology
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    • v.12 no.3
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    • pp.132-140
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    • 2022
  • This study was conducted to identify the optimal lotion manufacturing conditions with decursin and decursinol angelate of Angelica gigas Nakai extraction. Lotion was confirmed that it had viscosity (5,208±112 cPs), assay (99.71±1.01%), and pH (5.62) for 3 months. The optimization of manufacturing conditions of mixing 4 for lotion formulation were made by 22+3 full factorial design. Mixing temperature (40-80℃) and mixing time (10-30 min) were used as independent variables with three responses(assay, pH, and weight variation) as critical quality attributes (CQAs). The model for assay and weight variation identified a proper fit having a determination coefficient of the regression equation (about 0.9) and a p-value less than 0.05. Estimated conditions for the optimal manufacturing process of lotion were 61.93℃ in mixing temperature and 15.85 min in mixing time. Predicted values at the mixing temperature (60℃) and mixing time (20 min) were 100.69% of assay, 5.57 of pH, and 98.07% of weight variation. In the verification of the actual measurement the obtained values showed 100.29±0.98% of assay, 5.57±0.02 of pH, and 98.27±0.89% of weight variation, respectively, in good agreement with predicted values.