• Title/Summary/Keyword: 형질 특성

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Expression and Characterization of Three Types of $\delta$-Endotoxin Genes in Transformant, Bacillus thruingiensis PT0529 (형질전환체, Bacillus thuringiensis PT0529내에서 세가지 내독소 단백질 유전자들의 발현 특성)

  • 박현우;제연호
    • Journal of Sericultural and Entomological Science
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    • v.37 no.2
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    • pp.176-180
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    • 1995
  • To characterize expression and formation of three type crystal proteins in transformant, Bacillus thruingiensis PT0529 was analysed by transmission electron microscope and SDS-PAGE according to growth. The results showed that the introduced crystal protein genes, rcyIVD and cytA, were well expressed at earlier stage than resident crystal proteins were also expressed with their own morphology. However, resident crystal protein of B. thuringiensis PT0529 was smaller than that of wild type B. thuringiensis NT0423, suggesting that resident crystal protein production was interfered with introduced two type crystal protein genes.

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Study on the Physico-chemical Properties of Rice Grains Harvested from Different Regions (재배환경이 다른 쌀의 이화적적 특성에 관한 연구)

  • Kwang-Ho Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.2
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    • pp.234-242
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    • 1987
  • Rough rice samples of four rice varieties were collected from twenty five locations through the country just after 1986 rice growing season. Various characteristics related to rice grain quality were observed to clarify the degree of locational variation of physico-chemical properties, and cooking and eating quality of rice grains. Grain weight, grain shape, degree of translucency and chalkiness of rice grain, amylogram properties of rice flour, water uptake during cooking, and cooked rice appearance were different between varieties tested. High degree of locational variation were found in following characteristics, degree of translucency and chalkiness of rice grain, water uptake during cooking, cooked rice appearance and amylogram properties. Eating quality of cooked rice indicated by sensory score showed different tendency of locational variation between rice varie-ties tested, and locations produced rice grains showing better eating quality were not coinside with among varieties tested. Grain weight, degree of translucency and chalkiness of rice grain, and cooked rice appearance of rice samples showing better eating quality were quite different to rice grains showing poor eating quality. Rice having better eating quality of a japonica variety, Chucheong, showed higher value of peak and final viscosity, viscosity after cooling, consistency and set back on amylograph compared with those of poor eating quality rices, and break down value of better rice was lower than that of poor rice. However, a Tongil type variety, Taebaek, did not show any consistent difference between better and poor rices. Rice samples from six locations in Chucheong and four locations in Taebaek showed special properties on amylogram compared with other rices collected in this study.

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Studies of distribution of Eriocaulaceae on the Korean Peninsula (한반도 곡정초과(Eriocaulaceae)의 분포 연구)

  • Gil, Hee-Young;Chang, Kae Sun;Choi, Kyung
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.70-70
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    • 2018
  • 한국산 곡정초과 식물은 Nakai(1911)가 6분류군을 정리한 이래 지금까지 14분류군이 발표된 바 있다. 하지만 분류학적 형질이 총포, 포, 꽃받침, 꽃잎, 종자 등 미세형질에 집중되어 있고, 환경에 따라 식물체의 영양형질 변이가 커서 분류학적으로 어려운 분류군에 속하여 연구가 거의 이루어지지 않았다. 따라서 본 연구는 한반도 곡정초과의 분류학적 연구에 앞서 각 분류군의 과거 표본 기록 및 문헌 자료를 이용해 한반도내 분포 정보를 정리하여 분류군별 분포 범위를 제공하고 그 특성을 연구하고자 한다.

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Fine structural Changes in the Ele Epidermis According to Sea Water Adaptation. I Epithelial Cell (염분적응에 따른 뱀장어 표피의 미세 구조적 변화 I . 상피세포)

  • 박인식;김진정조운복박상옥
    • The Korean Journal of Zoology
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    • v.38 no.1
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    • pp.26-37
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    • 1995
  • 뱀장어, Anguilla joponicu의 표피를 구성하는 주종 세포인 상피세포는 80남 정도의 많은 당김세사를 함유하고 있어서 표피의 골격 유지에 중요한 역할을 하고 있다 회유행동 특성에 의해. 성숙된 뱀장어는 바다로 나가게 되고 표피는 급격한 환경변화를 서게 되는데 그 현상들을 살펴보면 먼저 상해반응으로 세포 내의 파립 형질내세망의 내강이 확장되는 현상과 다양한 크기의 공포의 증가로 인해 상피세포들 사이의 공간이 확장되며 일부 세포에서는 괴사 또는 변성되는 형태인 다층층판구조를 갖기도 한다. 이에 대한 능동적 대처로 부착반쪽으로 모이는 당김세사들이 일정한 방향성을 갖게 되며, 상피세포 사이의 연접부위에 부착반의 수가 증가되며 미토콘드리아. 형질내세망 등 세포소기 관이 발달되고, 분비과립의 증가 등 분비양상이 증가되고, 능동적인 염배출과 연관된 핵상부의 중앙축을 따라 미토콘드리아 및 과립 형질내세망이 풍부한 세포도 나타났다. 이와 같은 변화는 염분농도의 증가에 따른 환경적요인에 의해 일어나는 상피세포의 기능적 방어기작이라고 사료된다.

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A Study on the Morphological Characteristics of Leaves and Fruit of Cudrania tricuspidata in Korea (국내 자생 꾸지뽕나무 잎과 열매의 형태적 특성에 관한 연구)

  • Kwon, Yong-Seok;Park, Bo-Ram;Lee, Sol;Yu2, Han-Chun;Baek, So-Jin;Oh, Chan-Jin
    • Korean Journal of Plant Resources
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    • v.27 no.4
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    • pp.337-343
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    • 2014
  • This study was conducted to classify the variation in leaf and fruit morphology among natural grown 16 Cudrania tricuspidata stands. As results of the analysis in C. tricuspidata stands total 134 Species were found and as results of the analysis in leaf morphology, it was grouped as Group IV. By the analysis of variance in length, width, and weight of fruits were differed in 15 C. tricuspidata stands. The length, width, and weight of fruits were higher in Jinan and Sunchang, than Gochang, Gimje, and Goheung. By the cluster analysis of 3 fruit morphology were classified as 3 Groups. According to the results of this study C. tricuspidata leaves and fruit morphological characteristics for each group were compared by analysis there were differences between the morphological characteristics and the characteristics of the fruit did not match.

Estimation of Growth Curve Parameters for Body Weight and Measurements in Castrated Hanwoo (Bostaurus Coreanae) (한우 거세우의 체중 및 체형에 대한 성장곡선 모수 추정)

  • Choi, Te-Jeong;Seo, Kang-Seok;Kim, Si-Dong;Cho, Kwang-Hyun;Choi, Jae-Gwan;Hwang, In-Ho;Choi, Ho-Sung;Park, Chul-Jin
    • Journal of Animal Science and Technology
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    • v.50 no.5
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    • pp.601-612
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    • 2008
  • This study was conducted to figure out how the shape of Hanwoo changes over time, examine the rank correlations between the carcass traits which are the selection traits and parameters of growth curve, and determine the correlation between body shape and carcass. Body weight, body measurements and carcass traits were measured from 161 castrated Hanwoo, and 12 growth traits and 5 carcass traits were investigated in total. The logistic model(Nelder, 1961) used for the estimation of growth curve parameters and growth characteristics at inflection point were calculated by these growth curve parameters. The value of this parameter was greatest for pinbone width, which suggests that it is an early ripening trait, while it was lowest for chest girth, suggesting it to be a late ripening trait. The rank correlations of chest depth, chest width, and hip width with backfat thickness steadily increased from 6 to 24 months, while the rank correlations of other traits decreased after 18 months until 24 months of age. Only phenotypic records were analyzed in this study, but for examine the genetic changes over growth phase in Hanwoo, if another additional genetic analysis like as estimation of genetic parameters should achieve, body measurements may be useful traits in proven bull selection.

Physiological responses to drought stress of transgenic Chinese cabbage expressing Arabidopsis H+-pyrophosphatase (애기장대 H+-pyrophosphatase 발현 형질전환 배추의 건조스트레스에 대한 생리적 반응)

  • Jeong, Mihye;Kang, In-Kyu;Kim, Chang Kil;Park, Kyung Il;Choi, Cheol;Han, Jeung-Sul
    • Journal of Plant Biotechnology
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    • v.40 no.3
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    • pp.156-162
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    • 2013
  • Plant tolerance to drought is a beneficial trait for stabilizing crop productivity under water deficits. Here we report that genetically engineered Chinese cabbage expressing Arabidopsis $H^+$-pyrophosphatase (AVP1) shows enhanced physiological parameters related to drought tolerance. In comparison with wild type plants under soil water deficit stress created by cessation of irrigation, soil water potential in pot with AVP1-expressing plants was more rapidly decreased that might lead to increased relative water content in leaves, while both genotypes had indistinguishable wilting phenotypes. Transgenic plants subjected to drought treatment also exhibited higher photosystem II quantum yield in addition to lower electrolyte leakage and $H_2O_2-3,3^{\prime}$-diaminobenzidine content when compared to wild type plants.

Characterization of Transgenic Tall Fescue Plants Overexpressing NDP Kinase Gene in Response to Cold Stress (NDP Kinase 유전자를 과발현시킨 형질전환 톨 페스큐 식물체의 저온 스트레스에 대한 내성 특성)

  • Lee, Sang-Hoon;Lee, Ki-Won;Kim, Kyung-Hee;Yun, Dae-Jin;Kwak, Sang-Soo;Lee, Byung-Hyun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.4
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    • pp.299-306
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    • 2009
  • Oxidative stress is the main limiting factor in crop productivity. To solve global environmental problems using the plant biotechnology, we have developed on the oxidative stress-tolerant transgenic tall fescue plants via Agrobacterium-mediated genetic transformation method. In order to develop transgenic tall fescue (Festuca arundinacea Schreb.) plants with enhanced tolerance to multiple environmental stresses, nucleotide diphosphate kinase gene under the control of CaMV35S promoter were introduced into genome of tall fescue plants. Proteomic analysis revealed that transgenic tall fescue not only accumulated NDP kinase 2 protein in their cells, but also induced several other antioxindative enzyme-related proteins. When leaf discs of transgenic plants were subjected to cold stress, they showed approximately 30% less damage than wild-type plants. In addition, transgenic tall fescue plants showed normal growth when transgenic plants were subjected to $4^{\circ}C$ for 3 days treatments. These results suggest that transgene is important in ROS scavenging by induction of antioxidative proteins, and could improve abiotic stress tolerance in transgenic tall fescue plants.

Gene Expression in The Fifth Generation of TMV Resistant Transgenic Tobacco Plane at Elevated Temperature (TMV 저항성 형질전환 연초식물체 제 5 세대에서 유전자 안정성 및 고온조건에서의 유전자 발현)

  • 이기원;박성원;이청호;박은경;김상석;최순용
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.4
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    • pp.245-250
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    • 1998
  • Tobacco mosaic virus(TMV) coat protein cDNA was transformed to Nicotiana tabacum cv. NC82 and the transgenic tobacco plants resistant to TMV infection were isolated in the next generation. The expression of TMV coat protein cDNA and genetic stability of the fifth generation of TMV resistant transgenic tobacco plants at the higher temperature were investigated. The TMV coat protein cDNA was amplified by genomic PCR in all the TMV resistant transgenic tobacco plants. The TMV coat protein expressed in the transgenic tobacco plants was detected at very low level by immunoblot hybridization. Even in tansgenic plants that showed the viral symptom only on very late sucker growth (delay type plants), the coat protein expression in the suckers was much less than that of susceptible tobacco infected with TMV. The TMV coat protein expressed in the transgenic tobacco plants was below 0.01% of total protein. Transcription and expression of the coat protein cDNA in delay type plants were observbed at high temperature (38$^{\circ}C$), and TMV replication was suppressed at both 28$^{\circ}C$ and 38$^{\circ}C$. This indicates that unlike the resistance conferred by 'N' gene. TMV resistance of transgenic tobacco plant won't break down at high temperature.

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Growth and Yield as Affected by Vinyl Mulching and Sowing Time in Cassia tora L. (비닐피복과 파종기 이동에 따른 결명의 생육 및 수량)

  • 권병선;박희진;임준택;신동영
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.4
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    • pp.315-319
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    • 1990
  • In order to determine the optimum sowing date of Cassia tora L., ecological characters, components and yield were investigated under vinyl-mulching and non-mulching condition. Emergence date and maturation date of vinyl-mulching condition were earlier than those of non-mulching condition. especially with maturation date being about 30 days earlier, Under vinyl-mulching condition, length of stem and pod were longer, number of branches, pods and seeds per plant were increased, stem diameter was thicker, and weight of 1000 grains was heavier. Seed yield per 10a under vinyl-mulching was twice as high as that of non-nulching condition. As sowing date was delayed, the maturation date was progressively delayed. At sowing date of April 10, plants showed the longest in length of stem and pod, the largest in number of pods per plant and number of grains per pod, and the highest in weight of 1000 grains and seed yield. Judging from the results, optimum sowing of Cassia tora L. appeared to be April 10.

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