• Analytical Science and Technology
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• v.36 no.6
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• pp.332-339
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• 2023

This study aimed to verify the hypothesis that urea denatures hemoglobin in the blood, thereby exposing active sites of enzymes and enhancing the chemiluminescence of the blood-luminol reaction. When blood was pretreated with urea, higher concentrations of pretreatment urea or longer pretreatment times resulted in enhanced chemiluminescence in the blood-luminol reaction, supporting the above hypothesis. However, the chemiluminescence was enhanced when blood was treated with luminol mixed with an 8 M urea solution, although the fact that the time for urea to denature hemoglobin was shorter compared to when blood was pretreated with urea and followed by luminol. In addition, the chemiluminescence was enhanced when a transition metal without hemoglobin was reacted with urea-containing luminol. Based on these results, it is anticipated that urea not only denatures hemoglobin but also plays a role in the luminol-hydrogen peroxide reaction.

• Journal of Life Science
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• v.18 no.8
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• pp.1169-1172
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• 2008

The purpose of this study was to establish blood reference values for miniature pigs from various growth ages before pre-puberty. 12 miniature pigs from two swine farms were sampled by jugular venipuncture. Routine hematological and biochemical determinations were performed using whole blood and serum. For the variables examined in each age group the means and the standard deviations were calculated. In hematological values, there are significant changes (p<0.05) in RBC, hematocrit and platelet values in each group. In RBC and hematocrit values, 4-week group shows the lowest level, whereas in platelet values, 4-week group shows the highest level. In biochemical values, there are significant chnges (p<0.05) in glucose and GGT in each group, and in other biochemical values there are no significant changes during growing periods. The values presented here may be useful for knowing the normal values for miniature pigs and also screening the normal pigs in the early days of their life and during the developemental stages.

• Proceedings of the Korean Society of Veterinary Service Conference
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• 1997.05a
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• pp.249-250
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• 1997

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.2
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• pp.71-76
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• 2019

There have been no reports on the laboratory screening of congenital torticollis in equine medicine. The purpose of this study was to evaluate the blood chemistry of a newborn female foal with congenital torticollis. The filly was born after a normal parturition period, and the clinical pathology results were as follows: biochemistry of the plasma showed significantly higher levels of CK, LDH, and Na than those in a normal foal. However, Cl level was remarkably lower than that of the control. In conclusion, the cells in the neck muscles influenced the clinical pathological value in the neonate. A disorder of the muscles as well as the bone problem, such as the cervical vertebrae, may have caused congenital torticollis. To our knowledge, this is the first report detailing the clinical pathology results in a newborn foal with torticollis. These laboratory results can be used as a reference for interpretation of pathology results in foals.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.11a
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• pp.145-153
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• 2002

유해 화학 물질류에 의해 오염된 해양 환경 시료의 환경독성 수준을 평가하기 위하여 다양한 화학물질에 대해 민감성이 우수한 생물학적 독성평가기법을 개발 하고자하였다. 지속성 유기오염 물질 중 다환 방향 족 탄화수소(PAHs)를 처리한 광어(Conger myriaster)와 개조개(Saxidomus pupurata)의 DNA 손상정도를 single cell gel electrophoresis assay(comet assay)를 통해 분석하였다. PAHs 중 광양만에서 높은 농도로 검출되는 benzo(a)pyrene을 농도별(0, 10, 50, 100 ppb)로 처리한 후 2일과 4일에 광어의 혈액세포와 개조개의 근육세포를 채취해 comet assay를 실시하였다. benso(a)pyrene에 대한 DNA 손상정도를 처리된 농도와 생물종에 따라 다르게 나타났는데 광어의 혈액세포는 2일에 가장 DNA 손상정도가 높았고, 4일에는 회복되는 경향을 나타냈다. 개조개의 근육세포는 시간이 지나면서 DNA 손상정도가 증가하는 경향을 보였다. 이와 같은 결과는 comet assay 기법이 유해 화학물질로 오염된 해양생물 종의 환경독성을 검색하는 유용한 수단이 될 수 있음을 보여준다.

• Analytical Science and Technology
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• v.35 no.6
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• pp.242-248
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• 2022

The effect of ethylenediamine (EDA) on the reaction of luminol or Bluestar with blood and bleaches was studied. For this purpose, blood, chlorine bleach, and oxygen bleach were applied to filter paper, treated with EDA-containing luminol or Bluestar, and the changes in chemiluminescence intensity were observed. It was found that the chemiluminescence intensity of the luminol (or Bluestar)-blood reaction did not change with the increasing concentration of EDA. However, the chemiluminescence intensity of the luminol (or Bluestar)-chlorine bleach reaction decreased and the chemiluminescence intensity of the luminol (or Bluestar)-oxygen bleach reaction increased, with increasing EDA concentration. Thus, it was found that when EDA was added to luminol (or Bluestar), which is a blood-sensitive reagent, EDA suppressed the false-positive reaction induced by chlorine bleach and induced a false-positive reaction with oxygen bleach. Consequently, the addition of EDA is not recommended for enhancing bloodstains with luminol or Bluestar.

• Journal of the Korean Chemical Society
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• v.36 no.6
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• pp.947-954
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• 1992

Polyethylene glycol (PEG) grafted poly (γ-benzyl L-glutamate) (PBLG) was synthesized by the substitution reaction of PBLG and PEG having primary amino groups at both ends. PEG-g-PBLG films containing hydroxyl group were also prepared by the substitution reaction of PEG-g-PBLG film and ethanolamine (EA). Adhesion of platelets and activation of plasma proteins on the copolypeptide films were studied. The results showed that platelets are less adhered and activated on the PEG-g-PBLG than on other polypeptides and plasma recalcification time (PRT) on the PEG-g-PBLG was longer than that on other polypeptides. These results were consistent with those of blood clotting time and thrombus formation on the polypeptides. As a results, PEG-g-PBLG surfaces showed better blood compatibility than PBLG or PEG-g-PBLG-EA surfaces.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.264-265
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• 2000

수계환경의 오염 실상을 파악하기 위해서 어류의 혈액화학적 변화는 수중의 오염물질에 의해서 그 구성이 변동되므로, 수계환경의 오염수준을 감시하는 유용한 수단으로 활용되고 있다(Khattak and Hafeez, 1996). PCBs(Polychlorinated biphenyls)는 열에 안전하고 전기 절연성이 좋은 특성을 가지고 있어 1929년부터 미국의 Monsanto사에서 Arochlor란 이름으로 시판되어 상업적으로 이용되기 시작하였다(Hutzinger. et al., 1986). (중략)

• Polymer(Korea)
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• v.33 no.1
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• pp.84-90
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• 2009

Nitinol alloy (TiNi) has been widely used in vascular stents. To improve the blood compatibility of Nitinol alloy, its surface was chemically modified in this study. Nitinol was first coated with gold, then chemisorbed with L-cysteine (C/N), and followed by grafting of zwitter ionic poly(ethylene glycol) (PEG) (PEG-$N^+-SO_3{^-}$) to produce TiNi-C/N-PEG-N-S. The zwitter ionic PEG grafted on the Nitinol surface was identified by ATR-FTIR, ESCA and SEM. The hydrophilized surface was proven by the decrease of water contact angle. In addition, from the blood compatibility tests such as protein adsorption, platelet adhesion, and blood coagulation time, the surface-modified TiNi alloy exhibited a better blood compatibility as compared to the untreated Nitinol control. These results indicated a feasibility of synergistic effect of hydrophilic PEG and antithrombotic zwitter ion.

• Journal of the korean veterinary medical association
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• v.19 no.1
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• pp.62-70
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• 1983

This experiments was conducted in order to exermine the effect of fowl gizzard mucouse membrane dry powder on blood picture and its chemical value of mice (d d Strain). The data were obtained from 20 heads each female and male, which were allotted for 4 t