• Journal of Life Science
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• v.19 no.8
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• pp.1177-1182
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• 2009

In order to establish in vitro infection model for research of plant pathogen based on tissue softening disease in napa cabbage, eighty independent bacterial strains were isolated from the softened napa cabbage tissues. Eight bacterial isolates were primarily screened with the generation of reproducible tissue softening disease to fresh napa cabbages within 24${\sim}$48 hours after inoculation. Through various microbiological biochemical and morphological examinations, three Gram (-) isolates which harbor independent biological properties were finally chosen, and named as RBI, RB2 and RB6. Collective results obtained from API 20E test and analyses of VITEK 2 COMPACT and nucleotide sequences of 165 rRNA of each isolate proposed that isolates RBI and RB2 are close to the Erwinia carotovora subsp. odorifera, and RB6 is close to the Erwinia carotovora subsp. carotovora. These isolates grew optimally at $30^{\circ}C$ with neutral pH culture condition. The isolates caused softening tissue disease with dose-dependent manner regardless of pre-surface damages of napa cabbage. Minimum dose to cause soft rot disease for RBI, RB2 or RB6 were $8.0{\times}10^8$ CFU/mt $10^9$ CFU/ml or $4.7{\times}10^6$ CFU/ml respectively. These isolates caused tissue softening disease to eggplant, paprika and napa cabbage out of 14 different tested vegetables, indicating that these isolates damages specific plant tissues. The bacterial isolates obtained in this research and in vitro plant infection model will be adapted in the understanding of the mechanism of pathogenesis by plant pathogen.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.127-128
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• 2003

본 연구는 퇴비화 과정 중 난용성 인산의 가용화와 무기태 인산의 변화를 알아보기 위하여 수행하였다. 비료와 퇴비중의 인산형태는 다른 성분들보다 토양에 흡착 또는 고정되거나 불용화 되는 양이 많아 작물의 흡수량이 적다. 시비된 인산의 흡수율은 낮고, 그 대부분은 난용화되기 때문에 토양에 축적되거나 세탈과 용탈에 의해 수질을 오염화시키는 주원인이 되고 있다. 퇴비화 과정중의 인산형태별 함량변화를 분석조사하여 작물에 시비되는 인산비료와 퇴비의 시용량을 적절하게 조절하여 인산의 과잉 시비량을 저감시키기 위한 연구이다. 돈분을 원료로 한 퇴비화 과정에서 단계별로 퇴비시료를 채취하여 총인산(T-P), 유효인산(Avail. -P)과 무기태인산분획별(Ca-P, Al-P, Fe-P)로 분석한 결과는 다음과 같다. 퇴적더미의 초기부피는 570L였으며, 약 2개월간의 퇴비화를 통해서 시료채취와 미생물등의 분해작용으로 최종부피는 430L정도로 감소하였다. 이는 초기의 부피보다 25% 감소하였다. 퇴적더미의 분해로 인한 용적밀도의 변화를 고려하면, 총인산 함량은 초기 약 17,500mg/kg에서 최종시료는 22,500mg/kg로 증가되었다. 또한 퇴비화가 진행됨에 따라 인산의 가용태가 증가하는 결과를 보였으며, 초기의 유효인산이 4,500mg/kg에서 최종시료에서는 8,900mg/kg으로 증가되었다. 그리고 무기태 인산분획별 인산의 형태별 변화를 조사한 결과, 퇴비화 과정 중 Ca-P의 경우 pH와의 중요한 상관관계를 갖고 있었다. 유기물분해를 통해 유리된 인산과 Ca은 난용태로 전환되는데, 초기의 약 10일 동안 Ca-P의 감소원인은 pH의 감소로 인한 Ca이 유리되는 정도가 낮기 때문인 것으로 해석된다. 초기 Ca-P형태의 인산함량은 11,900mg/kg으로 Fe-P와 Al-P보다 많았다. 또한 퇴비화가 안정화되어 부숙된 최종시료의 무기태 인산분획물 중 Ca-P는 18,000mg/kg로 증가하였으며, Ca-P>Al-P>Fe-P의 순 이었다. 그러나 Al-P와 Fe-P 형태의 무기태인산은 초기의 함량비율보다 다소 감소한 결과를 보였다. 결론적으로, 퇴비화과정 중 단계별 인산함량의 형태전환을 분석한 결과 총인산의 함량은 퇴비화가 안정화될수록 부피감소로 인한 인산함량이 증가하는 경향을 보였지만, 유기물질의 분해로 인한 원료내 인산의 형태가 불용태와 난용태에서 가용태 인산으로 전환되는 것을 도출하였다. 또한 무기태 인산분획물에서는 Ca-P 인산형태가 퇴비화가 진행될수록 증가한다는 결과를 얻었으며, Fe-P와 Al-P는 분해된 유기물의 킬레이트작용으로 감소되었다고 판단되며, 그 존재형태가 경쟁적임을 알 수 있었다. 따라서 화학비료와 퇴비의 시용이 병행될 경우에는 퇴비의 가용태 인산함량뿐만 아니라 무기태 인산의 함량을 분석한 후 인산질비료의 시비량을 조절해야할 것으로 판단된다.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.5
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• pp.555-561
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• 2005

A new graphical method was developed to separate two distinctive decay rate coefficients($k_1$ and $k_2$) at their respective degradable substrate fractions($S_1 and $S_2$). The mesophilic batch reactor showed $k_1$ of $0.151\;day^{-1}$ for wasted activated sludge(WAS), $0.123\;day^{-1}$ for thickened sludge(T-S), $0.248{\sim}0.358\;day^{-1}$ at S/I ratio of $1{\sim}3$ for sorghum and $0.155{\sim}0.209\;day^{-1}$ at S/I ratio $0.2{\sim}1.0$ for swine waste, whereas their long term batch decay rate coefficients($k_2$) were $0.021\;day^{-1}$, $0.001\;day^{-1}$, $0.03\;day^{-1}$ and $0.04\;day^{-1}$ respectively. At least an order of magnitude difference between $k_1$ and $k_2$ was routinely observed in the batch tests. The portion of $S_1$, which degrades with each $k_1$ appeared 71% for WAS, 39% for T-S, 90% for sorghum, and $84{\sim}91%$ at S/I ratio of $0.2{\sim}1.0$ for swine waste. Ultimate biodegradabilities of 50% for WAS, 40% of T-S, $82{\sim}92%$ for sorghum, and $81{\sim}89%$ for swine waste were observed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.5
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• pp.515-528
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• 1994

China Coastal Water(CCW) appeared in the neighbouring seas of Cheju Island has been analyzed and the results are as follows: The water with salinity below $32.20\%0$ in the neighbouring seas of Cheju Island in summer is thought CCW. During the period from April 1985 to August 1986, salinity of more than $34.00\%0$ was appeared in the Cheju Strait during January to mid May, but it decreased gradually from late May. CCW with salinity below $32.20\%0$ appeared in the Cheju Strait in early July and disappeared in October. The mean thickness of CCW in the Cheju Strait was about 25m and CCW had an influence of up to about 50m depth. And stratification structure of the Cheju Strait in summer was halocline between the surface and 50m depth and homogeneous salinity layer below 50m depth. It seems that although CCW appears in the wide area of neighbouring seas surface of Cheju Island in summer, the core of this CCW which has the lowest salinity flows into the Cheju Strait from the western seas of Cheju Island. This CCW flows out the east through the central part of the Cheju Strait and then seems to be transported further in the form of eddy.

• Journal of the Korean Society of Hazard Mitigation
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• v.6 no.3 s.22
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• pp.69-76
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• 2006

An hydraulic and hydrologic analysis procedure was proposed to reduce the inundation damage of highway in urban stream, that could contribute the EAP and Traffic control planning of Dongbu highway in the Jungrang stream basin which is one of the representative urban area in Korea. We performed the HEC-HMS runoff analysis, and the UNET unsteady flow modeling to decide the inundation reaches and their characteristics. The high inundation risk areas were of Emoon railway bridge and the Wollueng bridge, which are inundated in the case of 10 year and 20 year frequency flood respectively. We also analyze the inundation characteristics under the various conditions of the accumulation rainfall and the duration. Flood elevation at the Wolgye-1 bridge exceed over Risk Flood Water Level(EL.17.84 m) when the accumulation rainfall is over 250 mm and shorter duration than 7 hr. When neglecting backwater effect from the Han river, inundation risk are highly at the reach C2(Wolgye-1 br. ${\sim}$Jungrang br., left bank), C1(Wolgye-1 br. ${\sim}$Jungrang br., right bank), D(Jungrang br. ${\sim}$Gunja br.) in order, but when consider the effect, the inundation risk are higher than the others at the reach D2(Jungrang br. ${\sim}$Gunja br., left bank) and E(Gunja br. ${\sim}$Yongbi br.), which are located downstream near confluence.

• Journal of the Korea Society for Simulation
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• v.20 no.1
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• pp.79-85
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• 2011

The problem of evacuating pedestrians from a room or channel under panic conditions is of obvious importance in daily life. In recent years, several computer models have been developed to simulate pedestrian dynamics. Understanding evacuation dynamics can allow for the design of more comfortable and safe pedestrian facilities. However, these models do not take into account the type and state of mind of pedestrians. They deal with pedestrians as particles and the state of mind as a social force, which is represented by conservative and long-range interactions between individuals. In this study, I used the lattice model proposed in my previous study to explore the evacuation behavior of pedestrians with morality. In this model, three types of pedestrians are considered: adults, children, and injured people. Collisions between adults and children result in injured people. When the number of injured people continuously in contact with each other reaches a given value k, the injured people are removed from the lattice space. This situation is the same as that in which pedestrians start stepping over injured people. This behavior was interpreted as the morality of pedestrians. Simulations showed that the evacuation showed down and eventually became jammed owing to the injured people acting as "obstacles" in relation to the morality k.

• Journal of the Korean Society for Railway
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• v.20 no.4
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• pp.521-528
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• 2017

In this study, vertical loading tests were conducted to investigate the nonlinear behaviors of the fastening systems that have generally been used in the concrete track of domestic railway lines. Nonlinear load-displacement curve models were derived based on the test results. The uplift forces generated in the fastening systems were evaluated by applying the derived nonlinear models as well as the existing linear models. The influence of the factors on the maximum uplift force of the fastening system was analyzed through a parameter study on the distance between neighboring sleepers, the horizontal distance between the center of the bearing and the nearest fastening system from the deck end, and the height of the bridge girder. From the evaluation results it is known that, for economical track and bridge design, due to deck end deformation, it is necessary to consider the nonlinear behavior of the fastening system in the calculation of the uplift force of the fastening systems.

• Journal of the Korea Concrete Institute
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• v.23 no.1
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• pp.3-12
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• 2011

The structural behavior of continuous reinforced concrete deep beams is mainly controlled by the mechanical relationships associated with the shear span-to-effective depth ratio, flexural reinforcement ratio, load and support conditions, and material properties. In this study, a simple indeterminate strut-tie model which reflects characteristics of the complicated structural behavior of the continuous deep beams is presented. In addition, the reaction and load distribution ratios defined as the fraction of load carried by an exterior support of continuous deep beam and the fraction of load transferred by a vertical truss mechanism, respectively, are proposed to help structural designers for the analysis and design of continuous reinforced concrete deep beams by using the strut-tie model approaches of current design codes. In the determination of the load distribution ratio, a concept of balanced shear reinforcement ratio requiring a simultaneous failure of inclined concrete strut and vertical steel tie is introduced to ensure a ductile shear failure of reinforced concrete deep beams, and the primary design variables including the shear span-to-effective depth ratio, flexural reinforcement ratio, and concrete compressive strength are implemented after thorough parametric numerical analyses. In the companion paper, the validity of the presented model and load distribution ratio was examined by applying them in the evaluation of the ultimate strength of multiple continuous reinforced concrete deep beams, which were tested to failure.

• Journal of the Korean Electrochemical Society
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• v.9 no.2
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• pp.89-94
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• 2006

Nafion/poly(ether(amino sulfone)) acid-base blend polymer electrolyte membranes were prepared and their proton conductivity and dimethyl ether permeability were investigated. Characteristics of direct dimethyl ether fuel cell (DDMEFC) performance using prepared blend membrane were studied. The increase of amine groups in the base polymer in composite membranes resulted in the decrease in dimethyl ether permeability. The proton conductivity of the blend membranes gradually increased as increasing temperature. The conductivity of Nafion/PEAS-0.6 (85:15) blend membranes was measured to be $1.42\times10^{-2}S/cm\;at\;120^{\circ}C$ which was higher than that of the recast Nafion. The performance of direct dimethyl ether fuel cell (DDMEFC) using the Nafion/PEAS blend membranes was higher than that using $Nafion^(R)115$ membrane. Enhanced performance of direct dimethyl ether fuel cells using Nafion/PEAS blend membrane was explained by reducing dimethyl ether (DME) crossover through the electrolyte membrane and maintenance of the proton conductivity at high temperature.

• Journal of the Korean Geotechnical Society
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• v.20 no.9
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• pp.133-144
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• 2004

Pre-reinforcement ahead of a tunnel face using long steel or FRP (Fiberglass Reinforced Plastic) pipes in NATM(New Austrian Tunnelling Method), known as the RPUM(Reinforced Protective Umbrella Method) or UAM (Umbrella Arch Method), is the promising method to sustain the stability of a shallow tunnel face and reduce the ground settlements. In addition, horizontal reinforcing of the face is recently emphasized to improve the stability of the face. However, the characteristics on longitudinal arching around the face have not yet been established quantitatively with the RPUM (crown-reinforcing) and/or the face horizontal reinforcing. In this study, therefore, the behavior of cohesionless soil around the face reinforced by the reinforcing member representing the RPUM and horizontal reinforcing is investigated through two-dimensional laboratory model tests. A series of tests were carried out on various conditions by changing lengths and angles of the reinforcing members. Based on the vertical pressure around the face, the characteristics of longitudinal arching have been found for the case of the non-reinforced and the reinforced.