• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.796-800
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• 2014

A feeding trial was conducted to investigate the growth and body composition of juvenile sea cucumber Apostichopus japonicus in integrated culture with abalone Haliotis discus hannai or rockfish Sebastes schlegeli. Triplicate groups of sea cucumber averaging $1.2{\pm}0.05g$ were cultured alone or with abalone or rockfish for 12 weeks. Survival of sea cucumber was not affected by co-culturing (P>0.05). Weight gain of sea cucumber cultured with rockfish was significantly higher than that of sea cucumber cultured alone (P<0.05), and did not differ from that of those cultured with abalone (P>0.05). These findings indicate that co-culturing sea cucumber with rockfish effectively improves the growth of sea cucumber.

• Journal of the Korean Applied Science and Technology
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• v.34 no.2
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• pp.338-348
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• 2017

Protein hydrolysates derived from plants and animals having antioxidant, suppression of hypertension, immunodulatory, alleviation of pain, and antimicrobial activity has been known as playing important role like hormone. This study was performed to optimize the hydrolysis of protein of sea of cucumber by a flavourzyme. The ranges of processes were the reaction temperature of 40 to $60^{\circ}C$, pH 6 to 8, and enzyme concentration 0.5 to 1.5%(w/v). As a result, the optimization of process was determined at temperature of $48-50^{\circ}C$, pH of 7.0-7.2, and enzyme concentration of 1.0-1.1%(w/v), and degree of hydrolysis was 43-45 at above conditions. The molecular weight of hydrolysate was distributed to 500-3,500 Da and showed typical peptides. Inhibition concentration ($IC_{50}$) of peptides of DPPH radical scavenging activity, Superoxide anion radical scavenging activity, Hydroxy radical scavenging activity, $Fe^{2+}$ cheating activity was 1.25, 3.40, 10.3, and 22.11 mg/mL, respectively. Therefore, we expect that those products are useful as functional food ingredients.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.331-336
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• 2011

The present study investigated survival and growth of the abalone, Haliotis discus hannai and sea cucumber, Stichopus japonicus co-cultured and supplied with abalone formula feed (AFF) and dry sea tangle (DST) in indoor tank. During the experimental period from April 20 to July 15 in 2011, we used abalone with shell length of $28.54{\pm}1.23mm$ and total weight of $2.92{\pm}0.26g$. The sea cucumber used in the experiment weighed $3.0{\pm}0.2g$ in wet weight. For the experiment, 3 types of feeds including AFF, DST and AFF-DST mixed (1:1) group were supplied to abalone and sea cucumber. During the experiment, survival of abalone reached over 97.0% and 87.0% for sea cucumber in three experimental tanks. Survival of the abalone was found to be higher in DST group, while it was lower among sea cucumber, although the survival was not significantly different among three experimental group. Growth of abalone fed with AFF was significantly higher, while the growth rate of sea cucumber was highest in the tank fed with AFF+DST mixed diet (P < 0.05).

• Applied Biological Chemistry
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• v.24 no.2
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• pp.126-131
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• 1981

General composition, amines, and flesh tissue of boiled-dryed abalone and sea cucumber were compared with those of the freeze-dried abalone and sea cucumber. No significant differences were found in the general composition between the boiled-dried and freeze-dried, but the slight amount of soup flowed out from the boiled-dried. A specific feature is that 28.24% of ash was found in the boild-dried sea cucumber and 33.99% of ash in the freeze dried one in order of Na, K, Mg, Ca. Such contents of ash are considered to be due to salt and soft bone of samples. In the changes of amines in abalone and sea cucumber, trimethylamine oxide (TMAO) showed no great difference between raw samples and the dried ones, but showed the decrease of 43% to 54% in the boiled-dried ones, compared with raw ones. Trimethylamine (TMA) was richer in the boiled-dried than in the dried. Dimethylamine (DMA) which has tendency of causing cancer in the human body showed 14 times of content in the freeze boiled-dried abalone, 18.5 times in the boiled-dried sea cu cumber and also showed 4.7 to 6.7 times of increase in the freeze dried, respectively compared with raw ones. The muscular change of abalone was not found in at freeze dried samples, but a considerable change in sea cucumber.

• Journal of Aquaculture
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• v.22 no.1
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• pp.118-121
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• 2009

This experiment was conducted to evaluate the effects of different substrates in rearing tank on growth and body composition of juvenile sea cucumber Apostichophus japonicus. Sea cucumber (average weight 3.6) were randomly distributed at a density of 10 juvenile per tank into nine tanks providing three different substrates including free-substrates, sand and wave-shaped plastic sheet as (shelter). Juveniles were fed with a commercial feed once in every 2 days for 8 weeks. At the end of the experiment, survival of sea cucumber was not affected by the presence of substrates (P>0.05). Weight gain and specific growth rate of sea cucumber reared in the tanks with sand were significantly higher than those of sea cucumber reared in the tanks with shelter and without substrate (P<0.05). Crude protein and crude lipid of whole body in sea cucumber reared in free-substrate tanks were significantly higher than those of juvenile reared in the tanks with other substrates (P<0.05). Higher ash content was obtained in sea cucumber reared in the tanks with sand compared to that of juvenile reared in the tanks with other substrates (P<0.05). The results of this study suggest that growth and proximate composition of whole body of sea cucumber was significantly affected by substrates. Among materials tested, sand may be a proper substrate for sea cucumber culture.

• 식품문화 한맛한얼
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• v.3 no.2
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• pp.182-186
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• 2010

• Journal of Aquaculture
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• v.12 no.1
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• pp.39-45
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• 1999

In order to obtain the basic biological data for artificial seedling production of sea cucumber, Stichopus japonicus, the influence of water temperature and air dry for spawning induction, egg development and larvae rearing was investigated during the period from April, 1995 through September, 1995. Spawning induction rate by the water temperature was 6.0~17.5% and air dry was responsed 1.4~4.0%. Number of eggs spawned of Stichopus japonicus were $50~500\times10^4$ individuals, the fertilization and hatching rate were ranged 84.0~96.0%, 71.4~84.6% respectively. The fertilized egg of Stichopus japonicus appeared mean diameter of $154{\mu}m$. At a constant water temperature of $23^{circ)C$, it become 4 tell stage from fertilization after 2 hours 10 minutes, hatching larvae after 14 hours half, auricularia larvae after 3 days, doliolaria larvare after 11 days and pentactula larvae after 15 days ready for settlement. The suitable food in the larvae reared for 17 days after fertilization were shown the best growth and survival in the larvae food of Chaetoceros calcitrans. Optimum density for larvae rearing were maintained of the larval density lower than 2 individuals/ml.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.5
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• pp.605-611
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• 2012

Sea cucumber, $Stichopus$ $japonicus$, is used not only as an outstanding tonic food but also as a traditional medicine for the treatment of asthma, hypertension, rheumatism, anemia, and sinus congestion. The purpose of this study was to examine sea cucumber as an anti-gastritis and anti-gastric ulcer in HCl-ethanol-induced gastric and $H.$ $pylori$-infected animal models. Thirty 7-week-old SD rats and Mongolian gerbils were divided into normal (Nor, n=6), control (Con, 60% HCl-ethanol+water, n=6), groupI (DSCI, 60% HCl-ethanol+sea cucumber 30 mg/kg, n=6), groupII (DSCII, 60% HCl-ethanol+sea cucumber 100 mg/kg, n=6), and group III (DSCIII, 60% HCl-ethanol+sea cucumber 300 mg/kg, n=6). Sea cucumber significantly suppressed gastric lesions and ulcers in the 60% HCl-ethanol-induced gastric model. Especially, 100 mg/kg of sea cucumber showed significantly inhibitory effects. In histopathological analysis of the $H.$ $pylori$ model, we found that sea cucumber augmented the eradication rates of $H.$ $pylori$ and attenuated gastric ulcer formation. Our results suggest that sea cucumber has inhibitory effects on gastritis and gastric ulcers. In addition, sea cucumber can be applied for the treatment of $H.$ $pylori$.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.5
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• pp.1407-1416
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• 2016

This study was conducted to investigated the effects of stocking density on growth and survival of sea cucumber, Apostichopus japonicus in the indoor multiple tank for 12 weeks. There were six treatments(stocking densities) in this experiment, i.e. 1, 2, 3, 4, 5 and 10 individuals per 10 L plastic aquarium($W41{\times}H24{\times}D11cm$, represented as D1, D2, D3, D4, D5 and D10, respectively). Each treatment had ten replicates. The results showed growth variation of sea cucumber, Apostichopus japonicus decreased with the increase of stocking densities. After the end of the experiment, no significant differences(P>0.05) between D1~D5 treatment group whereas significant differences(P<0.05) between D1 and D10 treatment group. Survival rate showed D1~D10 were 90%, 90%, 100%, 95%, 94% and 72%, respectively. Especially, significant differences(P>0.05) between D1~D5 and D10 treatment group. The results indicated optimum of stocking density for sea cucumber, Apostichopus japonicus within five individuals per 10 L plastic aquarium($0.05individuals/m^2$).

• Proceedings of the Malacological Society of Korea Conference
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• 2000.11a
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• pp.6-6
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• 2000