• Korean Journal of Food Science and Technology
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• v.32 no.2
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• pp.477-480
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• 2000

This study was undertaken to investigate immunochemotherapeutic activity against tumor growth and cytotoxic activity against tumor cell lines. Phellinus linteus (PL), which was artificially cultured in Morus alba, prolonged significantly the survival rate of mice intraperitoneally implanted with sarcoma 180 and inhibited solid tumor growth on mice subcutaneously implanted with sarcoma 180. The acetone precipitate of water extract of PL was better than its water extract. However, PL showed little cytotoxic activity against tumor cell lines.

• Korean Journal of Food Science and Technology
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• v.54 no.2
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• pp.134-140
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• 2022

Kelp belongs to the brown algae family and has been reported to exert anti-cancer effects on some cancer types, however studies have not been reported on the anti-cancer effects of kelp extracts on melanoma. In this study, the anti-cancer effects of kelp extract in B16-F0 cells were investigated, and the underlying molecular mechanisms were assessed. Kelp extract was found to inhibit the proliferation of B16-F0 cells, induce cytotoxicity, inhibit cell colony formation, and induce DNA fragmentation and apoptosis. The molecular mechanism was found to involve kelp extract increasing the expression of cytochrome-c and activated caspase-9 in the intrinsic apoptotic pathway. In addition, kelp extract upregulated the expression of Fas-associated protein with death domain and activated caspase-8 in the extrinsic apoptosis pathway. Activation of caspase-9 and caspase-8 by kelp extract induced activation of caspase-3 and cleaved poly adenosine diphosphate-ribose polymerase, consequently inducing apoptosis. These data suggest that kelp extract represents a potential therapeutic agent for melanoma.

• Food Science and Preservation
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• v.21 no.6
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• pp.878-884
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• 2014

Increased antimutagenic and in vitro anticancer effects were observed by the fermentation process during Kochujang manufacturing. In order to confirm the increased functionality, wheat grain, first fermented wheat grains (FFWG), second fermented wheat grains (SFWG), final fermented wheat grains (FiFWG), red pepper powder (RPP), and commercial Kochujang (CK) were prepared. Kochujang manufactured with final fermented wheat grains and red pepper powder were further fermented for 15 days and 30 days. The antimutagenic effects were determined by counting the number of revertants in Salmonella Typhimurium TA100 against N-methyl-N'-nitro-nitrosoguanidine (MNNG, 1.0mg/mL). The final fermented wheat grains (52% inhibition) showed higher antimutagenic effects than the wheat grain (34%), and the commercial Kochujang showed the highest antimutagenic effects (55%). We tested the inhibitory effect on the growth of HT-29 human colon carcinoma cells and AGS human gastric adenocarcinoma cells by using MTT (3-(4,5-dimethyl-thiazol)-2,5-diphenyltetrazolium bromide) assay. The results showed that increased fermentation process continually increased the growth inhibitory effect on both cancer cells. The further fermentation for 15 days of the Kochujang product also increased inhibitory growth of the AGS cancer cells. In conclusion, the methanol extract from fermented wheat grains and commercial Kochujang showed sequentially increased antimutagenic and in vitro anticancer activity, and thus the final commercial Kochujang revealed the highest effect.

• KSBB Journal
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• v.16 no.6
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• pp.547-553
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• 2001

Glycoprotein from the water extract of dried root barks of slippery Elm was investigated for its anticancer immunostimulating activity, The glycoprotein contained molecular weight 15,000 to 500,000 Da, total carbohydrates 55.8 to 72.1%), total uronic acid 30.0 to 30.5%, and total proteins 5.0 to 6.1%. The anticancer immunostimulating activities were examined for both in vitro bioassays such as immune cell proliferation assay, mixed lymphocyte reaction (MLR), direct mitogenicity, T-dependent antibody production, and in vivo bioassays such as septic shock test and anticancer activity test in B16 melanoma transplanted mouse model. In vivo assay, the glycoprotein at the concentration of 3 mg/kg showed the best result that median survival time increased to about 140% in contrast to control groups.

• KSBB Journal
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• v.16 no.6
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• pp.554-561
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• 2001

Immunostimulating exopolysaccharides with anticancer activity produced by Enterobacter sp. SSYL(KCTC 0687BP), isolated from Chinese elm(Ulmus sp.) were investigated. The exopolysaccharide contains molecular weight 100,000 to 1,000,000 Da and total carbohydrates 43.0 to 70.8%, total uronic acid 7.1 7o 12.4%, and total proteins 15.4 to 20.6%. Compositions and contents of sugars in the exopolysaccharides are 10-30% glucose, less than 1% fructose, 10-15% galactose, 8-12% fucose, and 40-70% glucuronic acid. The anticancer immunostimulating activities were examined and proved with regard to both in vitro and in vivo bioassays. In vivo assay, the glycoprotein at the concentration of 0.3 mg/kg showed the best result that median survival time in creased to ca. 138.1% in contrast to control groups.

• Korean Journal of Head & Neck Oncology
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• v.31 no.2
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• pp.21-28
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• 2015

서론: 국소 진행성 두경부암 환자에서 선행 항암요법 후 동시 항암화학방사선요법은 원격 전이를 줄이고, 국소병변을 줄여 방사선 치료의 효과를 높이거나, 기관의 기능을 보존할 목적으로 시도된다. 선행 항암요법의 약제로 는 docetaxel, cisplatin, fluorouracil (DPF) 삼제요법이 가장 효과적인 것으로 알려져 있다. 선행 항암요법 후 동시 항암화학방사선요법과 표준치료인 동시화학방사선요법을 비교한 3상 연구들이 모두 선행 항암요법이 더 낫다는 결과를 보여 주지 못하였지만, 이 연구들은 충분한 환자를 모집하지 못하고 조기 종료된 불완전한 연구라는 한계가 있었다. 이에 저자들은 DPF 선행 항암요법 후 동시 화학방사선요법과 표준치료인 동시 화학방사선요법을 비교하는 메타분석을 시행하였다. 대상 및 방법: 체계적 문헌고찰을 통해 국소진행성 두경부암 환자를 대상으로 시행된 DPF 선행 항암요법 후 동시화학방사선요법과 현재 표준치료인 동시화학방사선요법을 비교한 5개의 3상 연구 결과를 분석하였다. 대상환자는 862 명이었고, 분석 결과 DPF 선행 항암요법 후 동시화학방사선요법은 표준치료와 비교하였을 때 반응률, 2년 및 3년 생존율, 2년 및 3년 무진행 생존율, 점막염 및 빈혈 발생 빈도에서 통계적으로 유의한 차이가 없었다. 하지만, 완전관해율과 3~4도의 백혈구감소증 및 혈소판 감소증의 빈도는 선행 항암요법 시행군에서 더 높았다. 결론: 국소진행성 두경부암의 치료에서 DPF 선행 항암요법 후 동시 항암화학방사선요법을 시행하는 것은 표준치료인 항암화학방사선요법에 비해 생존율 개선을 보이지 못하였다. 선행항암치료를 추가하는 것이 특정 환자군에서 효과가 있을지에 대해서는 추가적인 연구가 필요하다.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.557-563
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• 2011

Platycodon grandiflorum (Korean name, Doraji) has been widely used in traditional herbal medicine as an expectorant for pulmonary disease and a remedy for respiratory disorders in Asia. Here, we investigated the effects of BtOH extract saponin fraction of P. gradiflourm (PGS) on phagocytosis and anti-tumor activity with related cytokine productions in RAW264.7 macrophage cells. The results showed that PGS increased phagocytosis, anti-tumor activity, TNF-${\alpha}$ and nitric oxide (NO) production without direct tumor cell cytotoxicity. To further investigate whether NO is involved in anti-tumor and phagocytic activities of PGS, cells were co-treated with specific iNOS inhibitors, L-NIL (N6-(1-iminoethyl)-L-lysine, dihydrochloride), to block NO production. PGS decreased anti-tumor activity in L-NIL-treated cells, whereas phagocytic activity was not inhibited under the same conditions, indicating that the anti-tumor activity by PGS appears to be conducted by NO. These findings suggest that P. grandiflorum could be used a potential nutrition therapeutic agent for cancer patients.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.224-224
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• 1994

N-arylsulfonylimidazolenes를 항암효과가 우수한 화합물로 설정하여 이들 유도체를 합성하고 이들의 세포독성을 측정한바 있다. 그 이유는 2번 위치의 alkoxy기에 의하여 alkylating agent로서 작용할 수 있다는 개념과 생체 반응 후 생성될 것으로 예상되는 N-arylsulfonylimidazolidinone이 sulofenur와 유사한 작용을 갖을 것으로 기대 되었기 때문이다. 이에따라 N-arylsulfonylimidazolidinone 유도체의 항암효과의 측정이 관심의 대상이 됨에 따라 이들 유도체를 합성하고 이들의 세포독성을 관찰함으로써N-arylsulfonylimidazolidinone 유도체들의 구조 활성 상관관계를 관찰하고자 하였다. 합성은 출발물질을 N-arylsulfonyl-2-alkoxyimidazoline에서 시작 몇단계를 거쳐 목적하는 화합물을 합성 하였다.

• Korean Journal of Plant Resources
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• v.23 no.5
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• pp.399-405
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• 2010

Rosa rugosa has been used as a folk medicine with various pharmacological properties for a long time in Asia. Recently, it has been reported that the extract of fractions from different parts of Rosa rugosa have various pharmacological effects on diverse diseases including diabetes, inflammatory diseases and tumor. We investigated effects of fructus extracts of Rosa rugosa(RRF) and semen extracts of this herb(RRS) on macrophage to evaluate the possibilities as a biological response modifier. We showed increased effects on tumoricidal activity, phagocytic activity, TNF-$\alpha$ and NO production in RRF-treated groups without direct tumor cell cytotoxicity. RRS-treated groups increased direct tumor cell cytotoxicity at high dose without tumoricial activity except increasing of TNF-$\alpha$ release. These results provide further possibilities for the beneficial immunomodulating effects of RRF on immune system with relatively larger safety margin rather than RRS.

• Food Science and Preservation
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• v.13 no.1
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• pp.83-87
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• 2006

Gastrodia elata Blume is a major imp0l1ant medicinal resource in Korea. In order to confirm the biological activities of Gastrodia elata Blume, we carried out various in vitro assays. Of them, anti-oxidant and anti-tumor activities were detected from assays. The prototype of Gastrodia elata Blume extracts was used for 1he evaluation of DPPH, FRAP, hydroxyradical scavenging assay as anti-oxidant assays, as well as anti-tumor asctivities as wound assay and invasion assay. As a result, the prototype of Gastrodia elata Blume extracts showed potent anti-oxidative activity and anti-tumor activity in vitro. These above results suggest that 1he Gastrodia elata Blume extracts could have potential to alleviate oxidation process, cell motility activity, and tumorigenesis.