• Korean Journal of Plant Resources
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• v.26 no.1
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• pp.103-110
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• 2013

Lacquer has traditionally been used to varnish. Many reports have revealed that lacquer has anti-microbical, anti-cancer, and anti-inflammatory activities. However, urushiol and their derivatives were known as an allergen. Therefore, we expected that lacquer will be used as a good health-food source if its side effect was solved. Here we analyzed their allergy induced constituents by using the GC/MS and evaluated comparative concentration of lacquer, puerariae radix and their mixture extracts. Our results showed that lacquer extract has allergenic compounds and mixture extract with puerariae radix has relatively lower amount. However, lacquer/puerariae mixture extract has strong anti-allergenic effects on the RBL-2H3 cell and puerariae extract was blocked allergenic effect caused by allergenic constituents contained in lacquer.

• Applied Biological Chemistry
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• v.44 no.2
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• pp.133-135
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• 2001

• Journal of the Korean Applied Science and Technology
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• v.37 no.6
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• pp.1764-1772
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• 2020

This study is aimed at determining whether graviola (Annona muricata) beneficially influences immunoglobulin (Ig) E-mediated allergic reactions in human mast cells (LUVA cells). To examine the anti-allergic effect of graviola leaf extract (GLE), we treated antigen/IgE sensitized mast cells with extracts of various concentrations. In this study, the results of the in vitro model of IgE-mediated mast cell degranulation showed that GLE significantly inhibited the release of histamine, β-hexosaminidase, TNF-α, IL-4 and IL-6 in LUVA cells. Pretreatment with GLE suppressed the phosphorylation of antigen-induced Lyn and Syk, thus suppressing the downstream MAPKs pathways. The above results indicate GLE could suppress mast cell activation and allergic responses. Accordingly, it can be supported that GLE has the potential to be used as functional cosmetic material.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.7-7
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• 2018

Morinda 속 식물은 꼭두서니과(Rubiaceae)에 속하는 다년생 상록관목으로서 전세계 80여종이 분포하고 있다. 그중 노니는 2000년 전부터 동남아시아 지역에서 식품, 향신료, 음료, 자양강장제 등 다양한 분야에 사용되고 있으며 뿌리, 줄기, 잎, 열매의 식물체 전체 부위가 이용되고 있다. 특히 열매는 주스 등 다양한 제품으로 만들어지고 있다. 본 연구는 조직배양된 노니 부정근을 재료로 하여 항알러지의 효능평가를 수행하고 지표물질로 rubiadin을 선정하였으며 이물질에 대한 정제방법 및 구조분석을 통해 정성, 정량적 분석방법을 확립하였다. 항알러지 효능에 대한 측정 결과 노니 부정근 조추출물 $100{\mu}g{\cdot}mL^{-1}$에서 약 85%의 높은 발현 억제 효과를 보였고 이를 분획 추출한 후 분획물별로 histamine 및 $TNF-{\alpha}$ 발현 억제효과를 측정한 결과 헥산 및 메틸렌클로라이드층 분획물에서 효과가 우수하였다. 노니 부정근 조추출물이 함유한 유효성분들 중 항알러지 효과를 나타내는 지표물질을 선정하기 위하여 용매분획을 실시한 후 preparative LC로 분취하여 항알러지 효과가 높은 분획물을 얻었다. HPLC로 분취한 화합물을 GC/MS/MS, LC/MS-IT-TOF, UV/VIS spectrophotometer를 이용하여 최종적으로 rubiadin을 확인하고 이 화합물을 지표물질로 선정하였다. 이러한 결과는 노니 부정근의 고부가가치 기능성 소재로 이용되는데 도움이 될 것으로 기대한다.

• Journal of Life Science
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• v.21 no.12
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• pp.1721-1725
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• 2011

Mast cells are major effector cells associated with allergic responses. They are activated through the release of histamine, arachidonic acid, and proinflammatory cytokines. We investigated the effect of nodakenin, derived from the roots of Angelica gigas Nakai, on mast cell degranulation and on an allergic response in an animal model. We also investigated the effect of nodakenin on expression of multiple cytokines. Nodakenin suppressed the release of ${\beta}$-hexosaminidase, a marker of degranulation, as well as the expression of interleukin IL-4 and TNF-${\alpha}$ mRNA. Nodakenin inhibited the passive cutaneous anaphylaxis (PCA) reaction in ICR mice in a dose-dependent manner. These results suggest that nodakenin can inhibit mast cell degranulation through the inhibition of IL-4 and TNF-${\alpha}$ mRNA expression, and that nodakenin may potentially serve as an anti-allergic agent.

• The Journal of Korean Medicine
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• v.24 no.3
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• pp.65-71
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• 2003

목적 : 정천탕(定喘湯, JCT)이 IgE/mast cell로 유포되는 천식(喘息)등의 알러지성 질환에 대한 효과를 실험적으로 검증하고자 하였다. 방법 : IgE의 혈중농도와(in vivo) 분비 량을 측정하였으며(in vitro), 알러지 관련 유전자의 발현을 RT-PCR로 검색하였고, JCT의 수용성 복합 추출물이 compound 48/80로 유발되는 mast cell line(IC-2)의 histamine의 방출에 미치는 직접적인 영향을 측정하였다(in vitro). 결과 : 정천탕은 ovalbumin으로 유발시킨 마우스의 혈중 IgE량과 IL-4와 CD-40로 유도시킨 spleen B 임파구의 IgE 생성량도 현저하게 감소시켰다. 또한 INF-r의 발현은 증가시킨 반면, IL-4와 IL-6의 발현과 mast cell의 histamine 방출은 억제하는 것으로 나타났다. 결론 : 정천탕의 항알러지 효과를 실험적으로 확인 할 수 있었으며 임상적으로는 천식등 알러지성 질환에 사용 할 수 있을 것으로 사료된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.124-124
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• 2018

본 연구는 국내 생약중 도라지를 포함, 인삼, 당귀, 생강, 마 5가지를 선별하여 각 원물별로 항산화 활성 가장 높은 기준으로 증숙시킨 조건의 흑도라지, 흑삼, 흑당귀, 흑생강, 흑마를 확보하였다. 증숙시킨 5가지 생약은 다시 혼합 추출(BMHE; Black Mixed Herbal Extract)하여 증숙하지 않은 생약 혼합 추출물(MHE; Mixed Herbal extract)과 항산화, 항염 및 항알러지 활성을 비교하여 증숙공정이 생약의 생리활성에 미치는 긍정적인 영향을 바탕으로 피부향장 소재로 활용가능성을 확인하고자 연구하였다. 실험은 MHE와 BMHE를 각각 50% Ethyl alcohol로 추출하여 감압농축하여 생리활성을 평가하였으며, MHE와 BMHE 추출물에 대한 세포생존률을 확인한 결과 $1,000{\mu}g/ml$의 농도에서 각각 89.4%와 95.3%의 세포생존률을 보임에 따라 $1,000{\mu}g/ml$ 이하의 농도에서 HME와 BMHE 모두 세포독성이 거의 적은 것을 확인하였다. DPPH radical scavenging effects 항산화 활성에 대하여 MHE와 BMHE을 비교한 결과 농도 의존적으로 항산화 활성을 보였으며 $1,000{\mu}g/ml$의 농도에서는 각각 66.8%와 74.2%의 라디칼 소거능을 확인하였다. NO assay로부터 항염활성을 비교한 결과에서도 MHE와 BMHE 모두 농도 의존적으로 NO의 함량 감소시켰으며 $1,000{\mu}g/ml$로 처리한 농도에서는 59.5%와 64.7%의 억제율을 각각 보임에 따라 항염 효과도 우수한 것을 확인하였다. 항알레르기 효능 실험 중 하나인 5-LO 억제효능을 측정하여 ME와 BME의 항알러지 효능을 측정한 결과 상기의 결과와 마찬가지로 농도 의존적으로 5-Lipoxygenase를 억제하였으며, $1,000{\mu}g/ml$의 농도로 MHE와 BMHE를 처리하였을 시 5-Lipoxygenase 효소활성에 대하여 각각 76.2%와 83.5%의 높은 억제율을 확인 하였다. 이는 양성대조군인 EGCG를 $200{\mu}g/ml$의 농도로 처리한 9.10%에 근접한 억제율을 보임에 따라 항알러지 효과가 우수한을 확인할 수 있었다. 실험 결과를 바탕으로 BMHE가 MHE보다 항산화, 항염, 항알러지 활성이 우수한 것을 확인 할 수 있었으며, 이는 증숙기법이 생약의 생리활성을 높여주는 것은 물론 더 나아가 본 연구를 통해 확보된 5가지 흑도라지, 흑삼, 흑당귀, 흑생강, 흑마는 향후 피부향장 소재로 활용이 용이할 것으로 사료된다.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.2
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• pp.103-114
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• 2017

Gnaphalium affine D. DON (GA) has been used as a vegetable as well as a folk medicine in East Asia. The antioxidant and anti-complementary activity of GA extract (GAE) has also been reported. However, little is known about its anti-inflammatory and anti-allergic effect and mechanism of action. In this study, we evaluated the inhibitory effects of GAE on the production of inflammatory mediators such as NO, $PGE_2$, TLR4, eotaxin-1 and histamine. Our results suggest that GAE inhibits the production of NO and $PGE_2$ by inhibiting transcriptional activation via the involvement of iNOS and COX-2. The LPS-induced expression of Toll-like receptor 4 (TLR4) was also attenuated. In addition, GAE inhibited A23187-induced histamine release from MC/9 mast cells. It also inhibited the production of eotaxin-1 induced by IL-4. Collectively, these results suggest that GAE may have considerable potential as a cosmetic ingredient with anti-inflammatory and anti-allergic properties.

• Journal of Life Science
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• v.28 no.10
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• pp.1170-1178
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• 2018

The anti-inflammatory effect of brown alga Ecklonia cava is well known, and several phlorotannins have also been reported. In this study, major active components for anti-allergy and anti-inflammation were identified by NMR and MS analysis, and the levels of effectiveness were compared. Six major phlorotannins-phloroglucinol, eckol, eckstolonol, triphlorethol-A, phlorofucofuroeckol A, and dieckol-were isolated from the ethyl acetate fraction of E. cava. In order to analyze the major active substances in E. cava, antioxidant, anti-inflammatory, and anti-allergic effects were evaluated for the six separate substances. Antioxidant capacities of each phlorotannin were evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, where phlorofucofuroeckol A and triphlorethol-A had the highest radical scavenging capacity in respective radical scavenging assays. Phlorofucofuroeckol A exhibited the highest inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells among phlorotannins tested. Dieckol inhibited the release of ${\beta}-hexosaminidase$, a marker for the release of histamine in mast cells, in a dose-dependent manner in antigen-stimulated RBL-2H3 mast cells. Additionally, no cytotoxicities were observed at 1 and $2{\mu}g/ml$ in both phlorofucofuroeckol A and dieckol. These results suggest that phlorofucofuroeckol A and dieckol may play a key role in allergic inflammatory reactions.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.47 no.2
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• pp.99-105
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• 2021

This study was conducted to identify the efficacy related to skin barrier functions such as anti-inflammatory, anti-allergic, cornified envelope formation and the alleviation effect of keratinocyte damage caused by S. aureus using the callus extract, which was induced and extracted from the leaves of Gynostemma pentaphyllum in Ulleungdo Island. In order to confirm the anti-inflammatory effect of the G. pentaphyllum callus extract on the skin, the expression of inflammatory cytokines was investigated in primary epidermal keratinocytes (HEKa) activated with PAR-2 agonist, and the G. pentaphyllum callus extract showed IL-8, IL-25, and TSLP production inhibitory effect. β-Hexosaminidase assay using RBL-2H3 cells was performed to confirm the anti-allergic efficacy, and G. pentaphyllum callus extract showed the effect of inhibiting the release of β-hexosaminidase. In addition, G. pentaphyllum callus extract showed the cornified envelope formation effect in HaCaT cells, and through the co-culture experiment with HaCaT cells and S. aureus, it showed alleviation effect of keratinocyte damage caused by S. aureus. Therefore, G. pentaphyllum callus extract is considered to be a useful cosmetic material for improving skin barrier with anti-inflammatory, anti-allergic and alleviation effect of keratinocyte damage caused by S. aureus.