• Journal of Nutrition and Health
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• v.40 no.4
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• pp.327-333
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• 2007

The present study was carried out to evaluate the physiological effects of mushroom supplementation on blood glucose levels, lipid profile, and antioxidant enzyme activities in subjects with type 2 diabetes mellitus. Subjects were randomized into either a control group or mushroom supplementation group. Mushroom supplementation was provided 3 times a day for 4 weeks. We found that total dietary fiber intake was about 2.5 times higher (30.3 g vs. 12.3 g) in subjects receiving mushroom supplementation than in the control group. Two groups maintained the same food intake and amount of activity, exercise during the supplementation. We observed no difference in age, height, weight, BMI (body mass index), blood pressure between the groups. Nutrient intake did not differ appreciably between the two groups, except for fiber intake, during the supplementation. Fasting blood glucose levels and 2-hour postprandial blood glucose levels were significantly lower in those ingesting mushroom than in controls. Furthermore, the concentrations of low-density lipoprotein cholesterol were decreased significantly in the mushroom supplementation group. Small changes were observed in the concentration of total cholesterol, triglyceride, high-density lipoprotein cholesterol of those supplemented with mushroom, but these changes were not statistically significant. Activities of superoxide dismutase and catalase with mushroom supplementation were higher than in controls, but and glutathione peroxidase activity was not affected. The levels of thiobarbituric acid reactive substance of mushroom group were lower than control group, but were not significant. We conclude that addition of mushroom influences glycemic control and may be effective in lowering blood lipids and improving antioxidant enzyme activities. Accordingly, such effects may reduce risk factors for cardiovascular disease in patients with type 2 diabetes. However, to confirm these effects and to make dietary recommendations for patients with type 2 diabetes, further studies are necessary.

• KSBB Journal
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• v.27 no.6
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• pp.367-374
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• 2012

Cigarette smoking (SM) is considered to be well known environmental toxin which contributes to the onset of various diseases. SM cause direct lungs damage, activate lungs inflammatory responses, and in some cases leads to the development of lung cancer. Cytokines in injured starfish (Asterina pectinifera) is the potential changes in its expression during the regeneration process. Especially, expression of TGF-${\beta}1$ has increased in arm cut starfish extract after eight days. Also, starfish including saponin like the ginseng. Saponin is widely used in the world because of some effective pharmacological activities. Therefore, the current study was designed to elucidate the pharmacological activities of starfish extract against cigarette smoking induced damage in cell line and pulmonary tissue. We investigate that the effect of eight days starfish extract after arm cut (8d) and intact starfish extract on cell line and mouse lung injury by SM. In cell proliferation analysis, although cigarette smoking extract (CSE) was co-treated, the higher proliferation ability is shown in 8d treatment than intact starfish extract. 8d and intact starfish extract was directly transported to pulmonary cells through respiratory organ by nebulizer inhalation. In this case of cigarette smoking, the pulmonary structure was damaged and functions become abnormal. However, 8d treated groups showed similar with the control group compared with SM group. Among them, 8d was proved to be more effective than intact starfish extract. These results demonstrate that 8d could more protect pulmonary structure and function than intact starfish extract against cigarette smoking by ginseng like saponin and regulation of inflammatory cytokines.

• The Journal of Internal Korean Medicine
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• v.29 no.1
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• pp.90-103
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• 2008

Objective : This study wascarried out to understand the effect of Sopyung-tang (SPT) on blood glucose & antioxidant enzyme activities in streptozotocin-induced diabetic rats. Methods : SD rats were separated into three groups, each with 20 rats. Except the normal group, the other two groups were intra-peritoneally injected with streptozotocin 6mg/kg. The experimental group was treated with SPT extract 500mgkg/day for 4 weeks. The normal and control groups were treated with saline 500mg/kg/day for 4 weeks. Changes of plasma glucose level and body weight were observed. After4 weeks, liver and kidney weight, antioxidant enzyme activities, and survival rate were observed with histological changes on liver, kidney and pancreas. Results : In the experimental group, body weight and survival rate increased, while plasma glucose level decreased significantly. Liver and kidney weight, XOD activity decreased in the experimental group compared to the control. GSH-px and CAT activities andinsulin-immunoreactive granules in ${\beta}-cells$ increased significantly in the experimental group compared to the control. Conclusions : This study shows that SPT might be effective for treatment of diabetes and its complications, as well as reduction of oxidative stress.

• The Korean Journal of Food And Nutrition
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• v.33 no.5
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• pp.459-472
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• 2020

The purpose of this study was to evaluate the physicochemical components and antioxidant activities of wheat sprout powder prepared by the enzymatic hydrolysis and lactic acid bacteria treatment. The four kinds of pre-treatment were: no treatment (WP), treated with enzyme (WPE), treated with lactic acid bacteria (WPL), and treated with enzyme and lactic acid bacteria (WPEL) were applied to the wheat sprout powder. The WPEL had higher total free amino acid and essential amino acid content than the other samples. As for the volatile aroma of the wheat sprout powder, 29 types of compounds were identified in the WP and WPL, 28 types in the WPE, and 27 types in the WPEL, respectively. The total polyphenols and flavonoids contents, in the wheat sprout powder was enhanced with the enzyme and the lactic acid bacteria pre-treatment. The WPEL had highest DPPH radical scavenging activities. The overall acceptability was the highest at 6.24 points in the WPEL. Based on these observations, it was confirmed that the enzyme and lactic acid bacteria pre-treatment could improve the antioxidant activities and active component of the wheat sprout powder.

• Applied Chemistry for Engineering
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• v.23 no.5
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• pp.496-500
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• 2012

Arthrospira platensis (A. platensis) is an economically important filamentous microalgae. The isolation of mutants by chemical or physical mutagen is a clue for the strain improvement. In this study, effects of ultraviolet-B (UV-B) radiation on A. platensis were investigated. Cells (or microalgae) were exposed to UV-B (15 Watt, 254 nm) for 1, 3, 5, and 10 min, and resulting mutants were designated UM1, UM3, UM5, and UM10, respectively. Especially, the lipid content of UM5 mutant was considerably increased by 8~11 fold compared to that of wild types. Moreover, the carotenoid content and antioxidant enzyme (peroxidase and superoxide dismutase) activity were increased. It was shown that UV-induced mutants can accumulate bioactive compounds, which will be useful for the industrial production of valuable products.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.5
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• pp.391-399
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• 2010

This study was conducted to improve the antioxidative activity of instant noodles containing enzymatic extracts from Ecklonia cava (EEC). EEC has relatively better antioxidative activity than extracts from other indigenous plants in Jeju Island. The EEC (2.5 mg/mL) had 82.5% for the hydroxy radical, 78.4% for the DPPH radical, and 64.9% for the superoxide anion radical scavenging activities, and 65.2% for the cell viability (100 ${\mu}g/mL$). According to the texture of the dough, the DPPH free radical scavenging of uncooked instant noodles, sensory evaluation of cooked instant noodles, and turbidity of the cooking drip, the optimal EEC concentration was 1.8% for the instant noodles. The major amino acids in the instant noodles with EEC were glutamic acid (24.2%), proline (10.2%), valine (10.0%), and isoleucine (12.3%). The zinc and iron in the instant noodles were enhanced by adding 1.5-1.8% EEC. The antioxidant activity of instant noodles with EEC was 75.4% for the hydroxy radical, 74.1% for the DPPH radical, and 51.2 % for the superoxide anion radical scavenging activities.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.874-881
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• 2014

A laboratory experiment was conducted to determine chronic effects of waterborne copper exposure on rock bream Oplegnathus fasciatus using a panel of enzymes. The activities of the following biochemical biomarkers were determined at different concentrations of $CuSO_4$ for 10 and 20 days: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in plasma; antioxidant enzymes including glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in liver and gills; and acethylcholinesterase (AChE) in brain and muscle. After exposure to two $CuSO_4$ concentrations (200 and $400{\mu}g/L$), the activities of plasma ALT in the fish showed a tendency to increase with AST and LDH, depending on $CuSO_4$ concentration. Additionally, GSH levels and SOD activities significantly increased, depending on $CuSO_4$ concentrations in liver and gills. This involved the inactivation of reactive molecules formed during oxidative stress, which could provide protection against oxidative damage induced by $CuSO_4$. However, GPx and AChE activities significantly decreased with $CuSO_4$ in liver and gills. In conclusion, these enzymes may represent convenient biomarkers for monitoring heavy metal pollution in coastal areas. Such chronic exposure studies are necessary for improving our understanding of complementary or deleterious effects of pollutants, and for developing metal toxicity biomarkers.

• Journal of Life Science
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• v.25 no.6
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• pp.615-623
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• 2015

This study investigated the effects of dissolved cement powder on Zacco koreanus by analyzing morphophysiological changes. The gills exposed to dissolved cement powder developed abnormal shapes in their secondary lamellae and increased numbers of mucous cells after long-term exposure. Additionally, clubbing, edema, and exfoliation of the epithelial cells were observed in the secondary lamellae. In the kidney tissue, the space in Bowman’s capsule was widened, and in the integument tissue the arrangement of the dermis was irregular due to the thinned epidermis. These results suggest that long-term exposure to cement powder can significantly affect morphological change, resulting death. The activities of antioxidant enzymes and LDH tended to increase commensurately with the duration of cement exposure. It was concluded that up-regulated proteins were the stress proteins involved in myofibrillar-protein production and down-regulated proteins were involved in glycolysis and energy metabolism after the integument’s exposure to cement. According to these results dissolved cement powder is a serious threat to the survival of fish because it causes morphological changes and weakens physiological activity in Z. koreanus tissues.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.23-23
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• 2010

전북 서해안 새만금 지역에 조성되는 간척지 면적 28,300ha의 30%인 8,400ha 정도가 농업용지로 사용되는 계획이 확정됨에 따라 해당 용지를 효율적으로 사용 할 수 있는 다양한 활용 방안이 요구되고 있다. 대단위 농업지구가 들어설 예정지인 새만금 광활 간척지는 네덜란드와 유사하게 토양의 대부분이 미사질 양토로 이루어져있어 적용 가능한 화훼류를 적절히 선발하고 적합한 재배기술을 도입한다면 대규모 화훼제배 단지로서의 성장 가능성이 충분할 것으로 판단된다. 따라서 전북 농업기술원에서는 2004년부터 7년에 걸쳐 새만금 간척지의 농업적 활용도 제고와 경관농업 육성을 통한 관광자원화 가능성을 확인하기 위하여 72종의 원예작물 적응시험을 실시하였으며 22종의 자생식물과 튤립, 아이리스, 수선화, 히아신스, 참나리 등 5종의 구근 화훼류를 적합 화훼류로 선발하였다. '08~09년에는 내염성과 저온 및 풍해 저항성이 있는 아펠톤, 골든아펠톤, 네그리타, 프랑소와즈, 키스네리스 등을 간척지적용 가능 주요 튤립 품종으로 선발 하였는데 선발된 품종들을 대상으로 높은 염농도 하에서의 체내축적 무기성분을 조사한 결과, K와 Mg은 증가되는 경향이었으나 Ca은 감소되었으며, Cl이온은 현저하게 증가하였다. 또한 튤립체내의 산도 및 전기전도도와 항산화효소의 활성이 증가하였는데 스트레스 반응결과 유해산소가 체내에 축적되고, 이를 중화시키기 위하여 수소이온을 소모함으로써 체내의 산도는 상승된 것으로 추정되며 염분함량이 높을수록 삼투압에 의하여 뿌리에서 흡수는 양분이 적은 반면 잎 줄기의 증산작용은 동일하기 때문에 상대적으로 체내의 농도가 높아져 전기전도도가 상승된 것으로 판단된다. 식물 스트레스의 지표물질로 사용되고 있는 proline 함량도 토양내의 염도가 상승되면서 증가되었으며 엽록소 함량은 감소되는 경향을 보였다.

• Environmental Analysis Health and Toxicology
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• v.21 no.3 s.54
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• pp.245-253
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• 2006

We reported previously that glucagon decreased alpha- and pi-class glutathione S-transferases (GSTs) and microsomal epoxide hydrolase (mEN) protein levels in primary cultured rat hepatocytes. The present study examines the effects of Protein kinase A (PKA) inhibitor, KT5720, on the glucagon-mediated decrease in expression of GSTs and mEN. To assess cell viability. lactate dehydrogenase release and MTT activity were examined in hepatocytes treated KT5720. Cell viability was significantly decreased in a concentration dependent manner after incubation with KT5720 at the concentrations of 1 $\mu$M or above for 24 h, which was inhibited by the cytochrome P450 inhibitor SKF-525A. In contrast, another PKA inhibitor H89 (up to 25 $\mu$M) was not toxic to hepatocytes. The glucagon-mediated decrease in expression of alpha- and pi-class GSTs and mEH was completely inhibited by 25 $\mu$M H89 and attenuated by 0.1 $\mu$M KT5720. This study demonstrates that KT5720 may cause cytotoxicity in rat hepatocytes through cytochrome P450-dependent bioactivation. The present study implicates PKA in mediating the inhibitory effect of glucagon on expression of alpha- and pi- class GSTs and mEH.