• Korean Journal of Plant Resources
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• v.21 no.5
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• pp.357-361
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• 2008

The photosynthetic characteristics of three Ligularia species, L. fischeri, L. fischeri var. spiciformis and L. stenocephala under-tree cultivated in Dunnae-myeon, Hoengsung-gun were measured and compared. Total mean photosynthetic rate increased with increasing the light intensity up to 2,000 PPFD. Mean photosynthetic rate of L. fischeri increased to maximum value of $21.1{\mu}mol\;CO_{2}m^{-2}s^{-1}$ with increasing the light intensity up to 1,600 PPFD, but it was decreased at 2,000 PPFD. The highest photosynthetic rate was shown in L. fischeri var. spiciformis, followed by L. fischeri, and L. stenocephala. was the highest $27.39{\mu}mol\;CO_{2}m^{-2}s^{-1}$ among three species, followed by that of The differences in photosynthetic rates were significantly different among the species each measuring date. L. fischeri var. spiciformis was more adaptive species at the low elevated under-tree cultivated sites, such as, Sanchemaeul Dunnae-myeon Hoeyngseung-gun than L. fischeri and L. stenocephala.

• Journal of Korean Society of Forest Science
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• v.99 no.1
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• pp.131-135
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• 2010

This study was carried out to establish a proper cultivation condition and diagnose the drought-tolerance of three wild edible greens belonging to genus of Ligularia by using pressure-volume curves methods. The result of the original bulk osmotic pressure at maximum turgor ${\Psi}_{\circ}{^{sat}}$ was -0.8 MPa in Ligularia fischeri and L. stenocephala, which was somewhat lower than the value, -0.7 MPa, in L. fischeri var. spiciformis. In addition, the values of the osmotic pressure at incipient plasmolysis ${\Psi}_{\circ}{^{tlp}}$ in L. fischeri and L. stenocephala, both of which were -0.9 MPa, These were slightly lower than that of -0.8 MPa in L. fischeri var. spiciformis. On the other hand, it appeared that the values of maximum bulk modulus of elasticity $E_{max}$ of L. fischeri and L. stenocephala were approximately two times higher than that of L. fischeri var. spiciformis. However, There was a distinct difference between the values of the relative water contents in these three species. Therefore, Ligularia spp, occurring incipient plasmolysis in the high water contents, have a relatively low property of drought-tolerance, suggesting that growth of those Ligularia spp. are appropriate for relative moisture forest.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.12
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• pp.1758-1763
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• 2012

This study was conducted to compare fragrance and volatile chemicals of essential oils in Gom-chewi (Ligularia fischeri) and Handaeri Gom-chewi (Ligularia fischeri var. spicifoprmis). Essential oils were extracted by steam distillation of leaves of Gom-chewi (GC) and Handaeri Gom-chewi (HGC), after which samples were collected by solid-phase micro extraction and the compositions of the essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). The yields of the essential oils in GC and HGC were 0.12% and 0.04%, respectively, and the threshold levels of the essential oils in GC and HGC were 0.01% and 0.1%, respectively. There were 19 constituents of the essential oil of Gom-chewi: 14 carbohydrates, 4 alcohols, and 1 acetate, and the major constituents were L-${\beta}$-pinene (36.02%), D-limonene (25.64%), ${\alpha}$-pinene (24.85%) and ${\beta}$-phellandrene (5.39%). In the essential oil of HGC, 25 constituents were identified: 17 carbohydrates, 4 alcohols, 3 acetates, and 1 N-containing compound, and the major constituents of HGC were D-limonene (39.74%), L-${\beta}$-pinene (35.43%) and ${\alpha}$-pinene (11.94%). The minor constituents of HGC were ${\rho}$-cymene, ${\gamma}$-muurolene, ${\gamma}$-cadinene, germacrene D, ingol 12-acetate and butyl 9,12,15-octadecatriene and nimorazole were not identified in the GC essential oil. Overall, the results showed that the fragrance and chemical compositions of essential oils in GC and HGC differed, suggesting that both essential oils could be used for the development of perfumery products.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.17-17
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• 2021

곰취는 주로 쌈용, 장아찌, 나물용 등으로 이용되고 있으며 주로 자생 곰취의 일종인 '곤달비' 라고 하는 종이 많이 재배되고 있다. 일반 곰취에 비해 곤달비는 향이 진하지 않고 씁쓸한 맛이 적어 인기가 많은 종이다. 또한 농가에서는 엽수가 많아 수량이 높기 때문에 재배를 선호하게 된 종이기도 하다. 그러나 곤달비는 수확후 여름을 지나면서 고온건조기에 흰가루병과 식물체가 위축되는 로젯현상으로 잎이 작고 진록색으로 되거나 고사하여 다음해의 수량을 저하시키는 원인이 되고 있다. 이렇게 2~3년 재배를 하면 연작장애와 더불어 결주가 생기기 시작하고 수량이 급격히 감소하는 문제가 발생되는 실정이다. 이러한 문제점을 해결하기 위하여 흰가루병에 저항성이면서 세력이 우수한 한대리곰취와 일반곰취를 교배하여 흰가루병에 강하고 수량성이 뛰어난 계통을 선발하여 그 동안 '쌈마니', '다목이', '곰마니', '수마니', '그린베어' 등 5품종을 육성한 바 있다. 이번에는 용도 다양화를 위하여 잎이 매우 커서 김밥처럼 곰취 밥말 이용을 선발하여 품종을 만들고자 하였다. '대마니' 품종은 2007년 7월에서 9월까지 인공교배를 하여 종자를 얻었으며 2008년 3월에 파종하여 육묘후 포장에 정식하여 생육관리를 하였다. 2013년 생육 및 수량특성 조사에서 일반적으로 많이 재배되는 '곤달비' 종보다 우수한 7033-1번 개체를 선발하였다. 선발된 7033-1번 개체는 2014년부터 2020년까지 노지포장에서 증식되면서 고농G-7호로 계통화 되었으며 2019년부터 2년간의 생육특성 검정을 통하여 최종 선발하였다. 그리고 2020년 농작물직무육성 품종선정위원회에서 신품종으로 선정되어 '대마니(Daemani)'로 명명되었다. '대마니' 품종의 특성은 엽병귀의 색이 연녹색을 띠고 엽병에 털이 있으며 잎 뒷면에 털은 없으나 광택이 없고. 엽맥밀도는 보통이다. 쌈용, 데침쌈용, 묵나물용으로 이용이 가능하다. 특히 엽장과 엽폭이 39cm와 49cm로 '잎이 커서 밥말이용으로 적당하다. 주당 엽수는 54개로 '쌈마니' 품종보다 매우 적다. 주당 수량은 4,961g 으로 '쌈마니' 1,362g 보다 3배 정도 많았다. 추대시는 8월 28일, 개화시는 9월 16일로 '쌈마니' 품종보다 약 6일 정도 늦었다. 흰가루병 저항성 정도는 '쌈마니' 품종의 중도 저항성에 비하여 전혀 병에 감염되지 않는 강한 저항성을 보였다. 이와 같은 특성의 흰가루병 저항성 품종이고 잎이 큰 '대마니' 품종이 농가에 보급되어 출하된다면 밥말이용 등 용도다양화를 통한 새로운 소비창출이 가능할 것으로 기대된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.58-58
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• 2018

'다목이'는 일반 곰취를 모본으로 한대리곰취를 부본으로 교배하여 병에 강하고 수량성이 뛰어난 특성을 가진 곰취 신품종으로 고령지농업연구소에서 육성하였다. 경관농업은 청정 농산물의 생산, 가공과 서비스가 연계된 농업의 6차산업화를 대표하는 농업형태이다. 곰취를 이용한 산채류 경관농업을 구현하기 위해 '다목이'와 자생식물의 혼식을 통한 친환경 생산기술을 개발코자 하였다. 인공상토로 채운 화분에 곤달비와 삼백초, 백리향, 라벤더 등 국내 외 자생식물을 혼식하여 각 식물의 지상부와 지하부 생육을 조사하였다. 삼백초, 라벤더, 이질풀, 웜우드, 어성초는 '다목이'와 혼식하여 지상부 생체중 214.1~362.4g, 지하부 생체중 171.0~258.5g으로 생육이 대등하거나 양호한 결과를 보였다. 반면에 생강과 박하는 '다목이'의 생육량을 지상부 생체중 90.9~123.6g, 지하부 생체중 54.0~110.3g으로 현저히 감소시켰다. 백리향과 좀씀바귀, 제충국, 옥잠화는 '다목이'에 의해 생육이 극도로 저하되거나 고사하였다. 배초향은 '다목이'와 서로의 생육이 감소된 것으로 나타났다. 따라서 '다목이'와의 혼식은 경관농업의 목적에 따라 삼백초, 라벤더, 이질풀, 웜우드, 어성초가 적합한 것으로 생각되었다.

• The Korean Journal of Food And Nutrition
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• v.32 no.4
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• pp.284-293
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• 2019

This study investigated the volatile flavor composition of essential oils from Ligularia fischeri and Ligularia fischeri var. spiciformis. The essential oils obtained from the plants were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). 99.63% volatile flavor compounds were identified in the essential oil from the L. fischeri. The major compounds were (E)-3-hexenol (30.73%), longiverbenone (13.23%), viridiflorol (12.39%), ${\gamma}$-muurolene (7.32%), limonene (6.12%), and caryophyllene (${\beta}-4.24%$). 99.76% volatile flavor compounds were identified in the essential oil from the L. fischeri var. spiciformis. The major compounds were ledol (42.81%), (E)-15-heptadecenoic acid (33.91%), ${\beta}$-bisabolol (3.23%), viridiflorol (3.08%), and cis-${\alpha}$-farnesene (2.60%). Although the two plants are very similar, the chemical composition of the essential oils was significantly different in quality and quantity. In the case of L. fischeri., it has high contents of monoterpene and sesquiterpene. (E)-3-hexenol, longiverbenone, ${\alpha}$-phellandrene, and ${\alpha}$-myrcene were regarded as the characteristic odorants of L. fischeri, but they were not identified in L. fischeri var. spiciformis. Ledo, (E)-15-heptadecenoic acid, and ${\beta}$-bisabolol were regarded as the characteristic odorants of L. fischeri var. spiciformis, but they were not identified in L. fischeri. The ratio of limonene, ${\gamma}$-muurolene and viridiflorol can be used as an indicator to distinguish between these two plants.

• Journal of Forest and Environmental Science
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• v.26 no.3
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• pp.209-217
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• 2010

The volatile aroma of fresh leaves is one of main factor in taste of all the edible green plants. The volatile aroma in almost edible green leaves are suggested as essential oil compounds. Ligularia fischeri, Synurus deltoides, Ligularia fischeri var. spiciformis and Aster scaber are one of the favourable edible green plants in Korea. In this study, volatile aroma compounds from Ligularia fischeri and Ligularia fischeri var. spiciformis species were analyzed by the SPME/GC/MSD method. Ligularia fischeri had 78 volatile aroma compounds such as D-limonene(20.28%), ${\alpha}$-pinene(dextro, 14.15%), L-${\beta}$-pinene(12.85%), 3-carene, ${\beta}$-cubebene(10.39%), etc. Ligularia fischeri var. spiciformis had 83 volatile aroma compounds such as D-limonene(36.97%), ${\beta}$-cubebene(13.95%), L-${\beta}$-pinene(13.38%), ${\alpha}$-pinene(dextro, 4.76%), caryophylle-ne(3.33%) etc. Conclusively, the commom volatile aroma compounds in Ligularia fischeri and Ligularia fischeri var. spiciformis leaves were D-limonene, ${\alpha}$-pinene, L-${\beta}$-pinene, ${\beta}$-cubebene, Caryophyllene, ${\alpha}$-farnesene, terpinolen. However, the composition and amount of volatile aroma compounds were very different between the two species.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.638-647
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• 2010

The genetic relationships in 5 species of $Ligularia$ were investigated using RAPD (Randomly Amplified Polymorphic DNA) and ITS (Internal Transcribed Spacer) sequences analyses. In RAPD analysis, sixty three of 196 arbitrary primers showed polymorphism. The amplified fragments ranged from 0.2 to 1.6 kb in size. The dendrogram was constructed by the UPGMA clustering algorithm based on genetic similarity of RAPD markers. A total of 16 accessions were classified into 5 major groups corresponding each species at the similarity coefficient value of 0.77. In the ITS sequence analysis, the size of ITS 1 was varied from 248 to 256 bp, while ITS 2 was varied from 220 to 222 bp. The 5.8S coding region was 164 bp in lengths. Forty nine sites (10.2%) of the 478 nucleotides were variable, and the G+C content of ITS region ranged from 49.4 to 53.5%. In the ITS tree, five species of $Ligularia$ were monophyletic, and $L.$ $taquetii$ was the first branching within the clade. $Ligularia$ $intermedia$ formed a clade with $L.$ $fischeri$ var. $spiciformis$ (BS=79), and $L.$ $stenocephala$ and $L.$ $fischeri$ were also claded. Two data sets were congruent, except of the position of $L.$ $fischeri$ var. $spiciformis$.

• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.625-629
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• 2016

A new Gomchwi cultivar ‘Gommany’ was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2006 to 2011 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. Petiole trichome existed, but light of leaf back did not. Density of leaf vein was 5 degree in a newly developed cultivar ‘Gommany’. Plant height, leaf length, leaf width and petiole length were 47.7, 16.3, 21.2, and 31.4 ㎝, respectively in the 3 year of growth characteristics. Plant size was also higher than that of ‘Gondalbi’. Bolting and flowering time were Aug. 5 and Sept. 10, respectively. In contrast, Bolting time of ‘Gondalbi’ showed 25 days quite earlier bolting time compared to ‘Gommany’, Which led to earlier flowering time. ‘Gommany’ showed higher number of leaves (138) per plant compared to ‘Gondalbi’ (130). Furthermore, yield was 97% higher in ‘Gommany’ (1,575 g/plant) than in ‘Gondalbi’ (798 g/plant). ‘Gommany’ showed higher leaf thickness (0.69㎜) than ‘Gonalbi’ (0.46 ㎜), and consequently showed more hardness in leaf characteristics (24.3 ㎏/㎠) compared to ‘Gondalbi’ (23.0㎏/㎠). ‘Gommany’ showed higher resistance in the susceptibility of powdery mildew compared to ‘Gondalbi’. ‘Gommany’ cultivar was registered as a novel variety with number 90 in The Minister of the Korea Forest Service on December 2015.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.339-345
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• 2021

'Gondalbi' (Ligularia stenocephala (Maxim.) Matsum. & Koidz.) is the most cultivated Gomchwi species because of higher yield and low in aromatic flavor and bitter taste, But 'Gondalbi' is susceptible to powdery mildew disease and leaf shriveling after harvest in Summer. To improve powdery mildew disease resistance and post-harvest leaf shriveling problem in 'Gondalbi', 'Handeari-gomchwi' resistant to powdery mildew disease and having higher yield potential used as a paternal trait donor. Powdery mildew disease resistance and post-harvest leaf shriveling as well as agronomic performance of a new variety, 'Greenbear' were tested under field and green house conditions in Pyungchang, Korea from 2007 to 2016. Expression of both maternal and paternal characteristics in 'Greenbear' including purple colored petiole ears, glossy leaf and paternal, petiole trichome, absent at the back of a leaf were confirmed. Plant size and flowering time of 'Greenbear' were similar to check line, 'Gommany', while leaf number per plant and yield were higher in 'Greenbear'. 'Greenbear' has thinner leaves (0.66mm) compared to 'Gommany (0.69 mm)', and hardness appeared slightly higher in 'Greenbear(25.1 kg/cm²)'. The resistance to powdery mildew disease of the 'Greenbear' variety was slightly lower than that of the 'Gommany' variety, but somewhat showed high resistance.